et a receptor expression and function after sah

... et al 1994; Airaksinen et al 1999; Manie et al 2001; Airaksinen and Saarma 2002) It is also essential for ureteric branching in kidney formation (Towers et al 1998; Sariola and Saarma 1999) and ... sites adaptor proteins such as Grb10, Grb7, Shc and Src (Pandey et al 1995; Asai et al 1996; Pandey et al 1996; Airaksinen et al 1999; Encinas et al 2004) The Ret gene was mapped to human chromosome ... Schmidt-Kastner et al 1994; Gash et al 1995; Opacka-Juffry et al 1995; Martin et al 1996; Shults et al 1996) Further studies have demonstrated a neurochemical and behavioral improvement in unilateral...

Ngày tải lên: 05/10/2015, 22:31

... strands of the shRNA1 and shRNA2 designed from Caveolin-1 cDNA are GCAAGTGTACGACGCGCAC and AACCAGAAGGGACACACA, respectively As negative controls (scrambled shRNAs), shRNAcontrol1 (TAGCGACTAAACACATCAA), ... shRNAcontrol1 (TAGCGACTAAACACATCAA), shRNAcontrol2 (TATA GCGACTAAACACATCAA) and shRNAcontrol3 (AAAG AGCGACTTTACACACTT) were designed All constructs were verified by sequencing shRNA-expressing lentiviruses ... biochemical data The results are consistent with the findings of Garcia-Marcos et al [14] and Bannas et al [15] They observed, in rat submandibular glands and mouse lymphoma cells, that the P2X7 receptor...

Ngày tải lên: 19/02/2014, 00:20

... 153–208 37 Kawahara T, Kohjima M, Kuwano Y, Mino H, Teshima-Kondo S, Takeya R, Tsunawaki S, Wada A, Sumimoto H & Rokutan K (2005) Helicobacter pylori lipopolysaccharide activates Rac1 and transcription ... the Noxo1b and Noxo1c cDNAs, the following primer, ‘d’, 5¢-CCGCGT TCTCCCAAAGCT-3¢ and primer ‘c’ were used 5¢-GA AATCCCATCACCATCTTCCA-3¢ (forward primer) and 5¢-CCTTCTCCATGGTGGTGAAGAC-3¢ (reverse ... contained KOD-plus DNA polymerase (Toyobo, Osaka, Japan), 0.3 lm each primer, and lL of the first-strand cDNA from different human tissues (Human MTC panels I, II, and Fetal MTC panel; Clontech) as...

Ngày tải lên: 19/02/2014, 06:20

... 5¢-GTGGGCCGCTCTAGGCACCAA-3¢ and 5¢-CT CTTTGATGTCACGCACGATTTC-3¢; for HPRT, 5¢-GT AATGATCAGTCAACGGGGGAC-3¢ and 5¢-CCAGCA AGCTTGCAACCTTAACCA-3¢; for GAPDH, 5¢-ACTCC ACTCACGGCAAATTC-3¢ and 5¢-CCTTCCACAATGC CAAAGTT-3¢; ... 5¢-TCTGATGAGACCGTCACTGC-3¢ and 5¢-TC TCCTGGCTCTCTTTGGAA-3¢; for PAM, 5¢-CCTGG GGTCACACCTAAAGA-3¢ and 5¢-TGTAAGGACACAC CGGAACA-3¢; for Y1 receptor, 5¢-CTCGCTGGTTCTCA TCGCTGTGGAACGG-3¢ and 5¢-GCGAATGTATATCT ... 5¢-GCGAATGTATATCT TGAAGTAG-3¢ [15]; for Y2 receptor, 5¢-TCCTGGATTCC TCATCTGAG-3¢ and 5¢-GGTCCAGAGCAATGACTG TC-3¢ [15]; and for Y5 receptor, 5¢-CGCTTCCATCTCAA GCAGA-3¢ and 5¢-AAGTCGTCTACGCTGCCTCT-3¢...

Ngày tải lên: 15/03/2014, 09:20

... mechanism, and function Cell 2004; 116: 281–297 Tili E, Michaille JJ, Gandhi V, et al miRNAs and their potential for use against cancer and other diseases Future Oncol 2007; 3:521-537 Calin GA and ... nematodes to 11 members in mice and humans Let-7 miRNAs negatively regulate Ras, and are typical examples of miRNAs that are frequently downregulated in solid cancers such as lung, breast, gastric, ... or enhance translation [7, 8] In silico bioinformatics analyses has helped a lot in predicting the potential targets of miRNAs Elucidating miRNA-target genes and their mechanism of action has given...

Ngày tải lên: 08/08/2014, 17:20

... AILIM/ICOS: its expression and functional analysis with monoclonal antibodies Hybrid Hybridomics 2001, 20:293-303 Harigai M, Hara M, Fukasawa C, Nakazawa S, Kawaguchi Y, Kamatani N, Kashiwazaki ... erythematosus Arthritis Rheum 1999, 42:1644-1648 Yokoyama H, Takabatake T, Takaeda M, Wada T, Naito T, Ikeda K, Goshima S, Takasawa K, Tomosugi N, Kobayashi K, et al.: Up-regulated MHC-class II expression ... co-stimulatory receptor is essential for Tcell activation and function Nature 2001, 409:97-101 Tafuri A, Shahinian A, Bladt F, Yoshinaga SK, Jordana M, Wakeham A, Boucher LM, Bouchard D, Chan VS,...

Ngày tải lên: 09/08/2014, 07:20

... 5'-CCAAGGCCAACCGCGAGAAGATGAC-3' and β-actin reverse primer 5'-AGGGTACATGGTGGTGCCGCCAGAC-3' (GenBank accession number: M10277) Annealing temperatures were 55°C for JAM-C and 60°C for β-actin The absence ... days after intra-articular mBSA injection Synovial inflammation was significantly reduced in anti-JAM-C antibody-treated mice (d) Circulating levels of serum amyloid A (SAA) on days and after ... transduction interactions [7] JAM-C was initially described as an adhesion molecule localized at interendothelial contacts and as an integrin ligand mediating interactions between vascular cells and leukocytes...

Ngày tải lên: 09/08/2014, 10:20

... Abnormal serological data, haematuria, proteinuria, red blood cell casts 60 18 13 SS group 22 F Abnormal serological data, haematuria, proteinuria 60 14 23 F 25 Abnormal serological data, haematuria, ... serological data, fever, erythra 50 9 10 F 1.3 Abnormal serological data, GN 65 12 12 11 F 0.42 Abnormal serological data, erythra, pyuria 60 10 10 12 F 4.08 Abnormal serological data, haematuria, proteinuia ... Abnormal serological data, fever, defluvium, erythra 60 10 10 17 F 12 Abnormal serological data, fever, pleurisy, haematuria, proteinuia, pyuria 60 20 20 18 F Abnormal serological data, haematuria,...

Ngày tải lên: 09/08/2014, 14:22

... in the data analysis and interpretation, and assisted in manuscript preparation TM, HK, AN, PEL, and NM assisted in data interpretation and manuscript preparation All authors read and approved ... authors declare that they have no competing interests Authors' contributions TN designed the study, and carried out data analysis, interpretation, and manuscript preparation KT and YK participated in ... hours Afterward, the cells were stained with monoclonal antibody (mAbs) to CD19 and inducible costimulator-ligand (ICOSL), B cell-activating factor receptor (BAFF-R) or transmembrane activator and...

Ngày tải lên: 09/08/2014, 14:22

... 5’-GAAGTCATGCATGTCACGTTTCTC-3’ and 5’GACTCTTCAAAAGCCACTCATCTCT-3’ for TAC3; 5’-TACGGCGAAGCTGTGCATT-3’ and 5’-TCACACAAGGCCCACACTGA-3’ for TAC4; 5’-GTAGGGCAGGAGGAAGAAGATGT-3’ and 5’-CAAGGTGGT CAAAATGATGATTGT-3’ for TACR1; ... of data, performed the statistical analysis and drafted the manuscript EN participated to the contractile function studies and to the analysis and interpretation of data AB and PAR participated ... 5’-GAGGCCGATGACGCTGTAG-3’ and 5’-CAAGACGCTCCTC CTGTACCA-3’ for TACR2; 5’-ATATACCTGTCCACCGCAATGG-3’ and 5’-CGCTTCCAGAACTTCTT TCCTATC-3’ for TACR3 Expected amplicon sizes were 91, 110, 90, 85, 84 and...

Ngày tải lên: 12/08/2014, 11:22

... Kasinska and Sussan Kianpour, the secretarial assistance of Marie Colbert, and Gordon Gaschler for discussion and preparation of the manuscript Additional technical assistance was provided by Jagbir ... determine the expression and relevance of the chitinases BRP-39 and AMCase in cigarette smoke-induced airway inflammation and Nikota et al Respiratory Research 2011, 12:39 http://respiratory-research.com/content/12/1/39 ... isoflurane (Abbott Laboratories, Saint-Laurent, Quebec, Canada) using a rodent anesthetic machine (Penlon Limited Abingdon, England) and inoculated intranasally with 25 μg of HDM extract (Greer Laboratories,...

Ngày tải lên: 12/08/2014, 13:22

... (Midland, TX) and were labeled with fluorescein isothiocyanate (FITC) at both the 59 and 39 ends PRL-1 (59-GGC CAA CAG AAA AGA AGT GCA CTG AGG TTT ACC CCA TCC AGG TCA-39) and PRL-2 (59-TGG CAA ATA ... their statistical and bioinformatic data analysis support; Dr Tom Barber for the use of his laboratory and equipment; and Dr Zhong-Yin Zhang and Chad Walls whose collaboration made a large portion ... Short hairpin RNA siRNA Small interfering RNA SS Staining score SSC Standard saline citrate Stat Signal transducer and activator of transcription TAE Tris-Acetate-EDTA TBS Tris-Buffered Saline...

Ngày tải lên: 24/08/2014, 11:44

... interfaces within neuronal nAChR subunit combinations (compare Fig 2A C) So far, a- conotoxins selectively targeting mammalian a3 b2 (a- MII, a- GIC) a6 b2 (a- MII, a- PIA), a3 b4 (a- AuIB) and a7 (a- ImI) ... perisynaptically, and a less abundant population of a3 * nAChRs which contain a5 and b4 subunits and in some cases b2 subunits, and is localized at postsynaptic densities [18,96] Functionally, a rapidly ... Presynaptic nAChR modulating dopamine release in the rat striatum (A) Nicotine acts at somatodendritic nAChR in the substantia nigra pars compacta and at presynaptic nAChR in the striatum (B) a- Conotoxin...

Ngày tải lên: 19/02/2014, 12:20

... rostral to bregma and mm caudal to lambda A modified Leur-Loc syringe hub (2.6 mm interior diameter) was placed over the exposed dura and sealed with cyanoacrylate adhesive Dental acrylic was applied ... significantly decreased alpha expression in sham but not injured animals, while diltiazem and diazepam significantly decreased expression in both sham and injured animals D: Gamma expression was normalized ... Diltiazem, an L-type VGCC blocker, and DZ, a GABAAR agonist, had statistically identical effects on the expression of GABAAR subunits α1, α3, and γ2, normalizing γ2 and significantly increasing...

Ngày tải lên: 10/08/2014, 05:21

... luminal and glandular epithelium [9], in addition to the Kalpokas et al Acta Veterinaria Scandinavica 2010, 52:66 http://www.actavetscand.com/content/52/1/66 formation of functional glands Endometrial ... Kalpokas et al Acta Veterinaria Scandinavica 2010, 52:66 http://www.actavetscand.com/content/52/1/66 Page of are mediated through specific intracellular receptors, namely, the alpha estrogen receptor ... change Also hypertrophy, nonseasonal endometrial hypoplasia, and lymphatic lacunae were evaluated Page of Statistical analysis All variables were subjected to analysis of variance using a mixed...

Ngày tải lên: 12/08/2014, 18:22

... 22:295-296 Haase M, Bellomo R, Devarajan P, Schlattmann P, Haase-Fielitz A, NGAL Meta-Analysis Investigator Group: Accuracy of neutrophil gelatinaseassociated lipocalin (NGAL) in diagnosis and prognosis ... Kuroyanagi Y, Mori T, Nagaoka K, Sasaki H, Maruyama T, Hayakawa K, Shiroki R, Kurahashi H, Hoshinaga K: Serum neutrophil gelatinase-associated lipocalin as a predictor of organ recovery from delayed ... data file shows the association between donor parameters and serum NGAL and U-NGAL concentrations Abbreviations ADH: antidiuretic hormone; AKI: acute kidney injury; CADI: Chronic Allograft Damage...

Ngày tải lên: 14/08/2014, 08:21

... TGTTACCAACTGGGACGACA-3’ 5’- GCTGCCCTTCCACATCTTCT-3’ 5’- CGCGATGCAGAACTACGAAA-3’ 5’- CAGCCTCAGCCGAAACTACA-3’ 223 282 156 2.2.9 Whole cell lysate preparation and Western blot analysis After treatment of pancreatic ... Journal of Pharmacology and Experimental Therapeutics and Journal of Cellular and Molecular Medicine Yung-Hua Koh, Ramasamy Tamizhselvi, and Madhav Bhatia Extracellular signalregulated kinase ... presented as nanograms per microgram of DNA 2.2.5 DNA assay Determination of DNA concentration in the samples was performed fluorometrically as described by Labarca and Paigen (Labarca and Paigen,...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 21/01/2014, 08:20

... national institution, and annual displays of activity and strength are now celebrated in every village in the country Prizes and medals are eagerly competed for; our great national games are prosecuted ... of bone came away, and I had the elbow-joint excised, and my arm is still stiff I attribute all this to the fact that I had rowed very hard at Henley and in the fours, and was in fact what is commonly ... the race injured a small vessel at the heart: previous to that he had been a particularly strong muscular man Eleven years after the Boat-Race he was suddenly taken ill and died in a few days."...

Ngày tải lên: 14/02/2014, 21:20

... TCCTGGCAATCGTGGTT CAA and ACCAGCTGGGCCAACATTTC; collagen III: TGGACAGATGCTGGTGCTGAG and GAAGGCCAG 3696 CTGTACATCAAGGA; alpha smooth muscle actin (a- SMA): AGCCAGTCGCCATCAGGAAC and CCGG AGCCATTGTCACACAC; ... 5¢-GCTGTGGCAGCTACCTATGTCTTG-3¢ and 5¢-AGGTCGTCATCATCCCACGAG-3¢; TNF -a: 5¢-AACTCGAGTGACAAGCCCGTAG-3¢ and 5¢-GTAC CACCAGTTGGTTGTCTTTGA-3¢; IL-10: 5¢-CAGACCC ACATGCTCCGAGA-3¢ and 5¢-CAAGGCTTGGCAA CCCAAGTA-3¢; collagen I: ... AGCCATTGTCACACAC; and glyceraldehyde-3-phosphate dehydrogenase: 5¢-GGCACAGTCAAGGCTGAGAATG-3¢ and 5¢-ATGGTGGTGAAGACGCCAGTA-3¢ Immunocytochemical staining for NF-jBp65 MSCs in IMDM supplemented with 10% fetal...

Ngày tải lên: 18/02/2014, 04:20