estrogen related receptor amp 945 and osteopontin gene expression
Báo cáo khoa học: Estrogen-related receptor a and PGC-1-related coactivator constitute a novel complex mediating the biogenesis of functional mitochondria potx
Ngày tải lên :
06/03/2014, 09:22
... Visconti A, Minervini F & Caruso MG (2005) Oestrogen receptor -related receptor a (ERRa) and oestrogen receptors (ERa and ERb) exhibit different gene expression in human colorectal tumour progression ... the expression of antioxidant protection genes and mitochondrial function Biochem Biophys Res Commun 357, 231–236 Ariazi EA, Clark GM & Mertz JE (2002) Estrogenrelated receptor a and estrogen- related ... FTC-133, XTC.UC1 and RO 82 W-1, the expression of PRC and ERRa was correlated with the mitochondrial mass, the expression of mitochondrial genes and the activity of the COX and CS enzymes ERRa...
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Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx
Ngày tải lên :
18/02/2014, 16:20
... agonist and its binding to the receptor is indispensable 4-OHT exhibited reasonable receptor binding affinity for both the (275Ala)-ERRc and (316Ala)-ERRc receptors (Table 2) and, in the reporter gene ... (275Ala)-ERRc and (316Ala)-ERRc mutant receptors, and even for (Ala, Ala)-ERRc Discussion Differential capacity of Glu275 and Arg316 to interact with the ligand Fig Biological activity of the ERRc and ... selecting and binding the ligand for receptor activation Of course, each individual NR should bind a specific ligand in a manner that differs from that by which other NRs bind their ligand, and thus...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
Ngày tải lên :
23/03/2014, 06:20
... oligonucleotide and analyzed the expression of Hox genes and cyclins As shown in Fig 6E, the knockdown of MLL1 down-regulated the expression of HoxA5, HoxA7 and HoxA10 genes Notably, HoxA5 expression ... the cell cycle (10 and 20 h), correlating with the expression of the gene (Fig 6C,D) The correlation of promoter occupancy of MLL1, H3K4 trimethylation and RNAP II with Hox gene expression indicates ... of MLL1 and H3K4 trimethylation in differential Hox gene expression during the cell cycle To further confirm the importance of MLL1 in the regulation of HoxA5, HoxA7 and HoxA10 genes and cell...
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Báo cáo khoa học: "Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks" ppsx
Ngày tải lên :
07/08/2014, 18:21
... ihsigeN ,J rehtnyG ,R erooM ,P iksokaknoH 21 191-971 ,8 ,9891 AND seneg tnereffid morf debircsnart sANRm 054P owt fo noitaluger :ANDc 054P emorhcotyc elbicudni latibrabonehp nekcihc a fo ecneuqeS ... fo emulov noitcaer lanif a ni tuo deirrac saw RCP slocotorp eht gnisu tuo deirrac saw sisehtnys ANDc dnarts tsriF ANR fo noitartnecnoc lµ/gµ htiw detcudnoc saw RCP-TR evitatitnauQ RCP-TR evitatitnauQ ... eht sa tes saw snoitidnoc gnilcycomrehT lm 52 fo emulov lanif eht ekam ot ebut hcae ni dedda saw ANDc fo elpmas lm eerhT ebut RCP hcae ni seititnauq lm 22 ni detouqila dna deraperp saw selpmas...
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Báo cáo y học: "ExpressionPlot: a web-based framework for analysis of RNA-Seq and microarray gene expression data" doc
Ngày tải lên :
09/08/2014, 23:20
... Friedman1,2,3* and Tom Maniatis4 Abstract RNA-Seq and microarray platforms have emerged as important tools for detecting changes in gene expression and RNA processing in biological samples We present ExpressionPlot, ... performs alignment and statistical analyses, and a web-based front end, which allows users to explore and further compare the completed analyses Compared to Galaxy and GenePattern, ExpressionPlot’s ... measures of quality control, plot changes in gene expression and RNA processing, browse hyperlinked tables of changed genes and splicing events, generate read plots from a genomic view, compare...
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báo cáo khoa học: "Antiproliferative factor decreases Akt phosphorylation and alters gene expression via CKAP4 in T24 bladder carcinoma cells" docx
Ngày tải lên :
10/08/2014, 10:20
... sense strand) and a scrambled doublestranded negative control siRNA (5’-AAUUCUGUAUG CUACCUGUAGAA-3’ sense strand) were prepared by preincubating single-stranded sense and antisense strands prepared ... qRT-PCR Gene expression was determined using SYBR® Green based real-time RT-PCR, QuantiTect® primers and reagents (Qiagen) and a Roche 480 LightCycler Samples were tested in triplicate runs, and ... protein expression and phosphorylation in cells electroporated in the presence of no siRNA (Lanes and 2), CKAP4 siRNA (Lanes and 4), or scrambled non-target (NT) siRNA (Lanes and 6), and treated...
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báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells" pdf
Ngày tải lên :
10/08/2014, 10:21
... methylation status and mRNA expression of FHIT and PTEN in Page of Hela cells and FHIT and CHFR in Siha cells in our study, even though both of these two genes might achieve high mRNA expression through ... and 38.04%, respectively Furthermore, the relative mRNA expression level of PTEN was higher than other genes Except for FHIT and CHFR, the mRNA expression levels of CCNA1, PAX1, PTEN, SFRP4 and ... lines, and suggested that they acted synergistically in inhibiting CpG island hypermethylation of tumor suppressor genes Rhee et al Figure Effects of DNMT1 silencing on gene methylation and mRNA expression...
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báo cáo khoa học: " The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression in vitro" ppsx
Ngày tải lên :
11/08/2014, 20:20
... the individual primers immediately before PCR amplification and SYBR Green fluorescence measurement for quantification of gene expression Samples were amplified in 96-well microplates in an IQ5-Cycler ... bisphosphonates on cyclin D1 gene expression Time-course experiments were performed to determine the effects of zoledronate, ibandronate and clodronate on cyclin D1 gene expression As shown in figure ... with ibandronate, zoledronate and clodronate did not significantly influence the gene expression of cyclin D1 during the first days Zoledronate, however, caused a decreasing cyclin D1 gene expression...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
Ngày tải lên :
12/08/2014, 02:20
... Differential chemokine, chemokine receptor, cytokine and cytokine receptor expression in pulmonary adenocarcinoma: diffuse down-regulation is associated with immune evasion and brain metastasis Int J ... (Figure 3, Table 3) In general, H5N1/2004 showed a higher capacity in inducing CXCL-10/IP-10 and CCL-5/ RANTES as compared with that of H1N1 and H9N2; Table Cytokine/chemokine mRNA expression during ... plasmids cloned with the M gene were used for standard curve construction The b-actin gene was used as an endogenous control for normalization [56] Cytokine/chemokine mRNA expression profile Total...
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Báo cáo y học: "Effects of short-term glucocorticoid treatment on changes in cartilage matrix degradation and chondrocyte gene expression induced by mechanical injury and inflammatory cytokines" doc
Ngày tải lên :
12/08/2014, 18:20
... Figure Effects of DEX on iNOS gene and protein expression and nitrite production A iNOS relative gene expression Values at the y-axis represent the expression level of each gene normalized to that ... sulfate (Sigma) as the standard [38] Page of 15 Gene expression studies: RNA extraction and real-time PCR To examine the effects of DEX, injury and TNFa on chondrocyte gene expression, bovine cartilage ... controls or TNFa-treated samples Effects of DEX, TNFa and mechanical injury on chondrocyte gene expression Real-time qPCR was performed to determine bovine chondrocyte gene expression responses to...
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Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
Ngày tải lên :
13/08/2014, 05:21
... A549 and NCIH292 cells were harvested at 3, 5, and 14 days, followed by DNA and RNA extraction, as described below Samples of the culture supernatant were collected at and days after infection and ... samples Transcripts of inducible nitric oxide synthase (iNOS) and IL12 p40 were detected in control A549 cells, and expression levels were not different between samples (Figure 2C) Cytokine gene ... Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cells Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in...
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Báo cáo y học: " Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+ T cells" doc
Ngày tải lên :
13/08/2014, 09:20
... regulatory subunit may be generally involved in constitutive gene expression in resting and activated CD4+ T cells, whereas Cyclin T1 may play a larger role in the expression of genes induced by T cell ... (see Methods) To generate a hierarchic illustration of the GO categories in biological process, nonredundant gene lists generated by GeneSifter and modified by removal of redundant genes were analyzed ... functions for HIV-1 gene expression as inferred from infections with the Tat+ and Tat- reporter viruses We therefore wished to investigate the global effects of prostratin on cellular gene expression...
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Báo cáo y học: "Induction of Bim and Bid gene expression during accelerated apoptosis in severe sepsis" potx
Ngày tải lên :
13/08/2014, 11:23
... score, correlated with pro-apoptotic Bim, Bid and Bax gene expression (Table 2) The severity of illness (SAPS II score) correlated with Bim and Bak gene expression (Table 2) Furthermore, mRNA expression ... on the one hand the BH-3-only pro-death gene expression of Bim and Bid was massively upregulated Furthermore, the mRNA of the pro-apoptotic Bak was increased On the other hand, the genes of the ... controls (a) The expression of BH3-only members Bim and Bid was upregulated, (b) the pro death-group Bak and Bax was regulated heterogeneously and (c) the anti-apoptotic Bcl-2 and Bcl-xl were...
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Báo cáo y học: "Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes" docx
Ngày tải lên :
13/08/2014, 14:20
... detectable in the samples treated with medium (Lane 1), DMSO (0.1%; Lane 2), AC (6.25, 12.5 and 25 μM; Lanes to 5), antiCD3/CD28 Ab (Lane 6), DMSO and anti-CD3/CD28 Ab (Lane 7), and AC and anti-CD3/CD28 ... CsA on IL-2, IFN-g and cell proliferation in T lymphocytes activated with anti-CD3/CD28 Ab To determine whether AC decreased NF-AT activation, gene expression of IL-2 and IFN-g and cell proliferation ... (2.5 μM), an NF-AT inhibitor, into T lymphocytes and analyzed gene expression of IL-2 and IFN-g as well as cell proliferation While IL-2 (P = 0.001) and IFN-g mRNA (P = 0.002) were significantly...
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IDENTIFICATION OF PUTATIVE TARGETS OF NKX2-5 IN XENOPUS LAEVIS USING CROSS-SPECIES ANNOTATION AND MICROARRAY GENE EXPRESSION ANALYSIS
Ngày tải lên :
24/08/2014, 12:29
... selected genes 89! Figure 3.9 – Standard curves for qPCR profiled genes 92! Figure 3.10 – qPCR expression profiles of selected genes (normalized to ODC) 98! Figure 3.11 – Expression ... up to a hundred thousand probes, potentially covering all known genes for an organism When multiple samples are compared, global changes in gene expression can be measured and 13 Figure 1.3 – ... annotated genes and input from the Xenopus laevis research community at large Gene Ontology The Gene Ontology project provides a common vocabulary to describe the characteristics of genes and their...
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DEVELOPMENTAL DIFFERENCES AND ALTERED GENE EXPRESSION IN THE TS65DN MOUSE MODEL OF DOWN SYNDROME
Ngày tải lên :
24/08/2014, 12:33
... bone formation (PROVOT and SCHIPANI 2005) Several genes and other molecules are known to regulate or affect both osteogenesis and mandibular development For example, Hox genes are thought to coordinate ... Dlx3, Dlx5, and Dlx5 are expressed in the mandibular processes, and Dlx5 and Dlx6 are also expressed in developing bones, cartilage, and teeth and may play an important role in mandibular formation ... a gene ontology number and then identifiers for each gene in the category Selecting the identifier for a particular gene would bring up all known processes related to that particular gene and...
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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" doc
Ngày tải lên :
20/06/2014, 04:20
... amylin, and calcitonin gene -related peptide on osteoclast development Bone 2001, 29:162-168 29 Chen FP, Hsu T, Hu CH, Wang WD, Wang KC, Teng LF: Expression of estrogen receptors alfa and beta mRNA and ... capsaicin and resiniferatoxin Biochem J 1995, 307(Pt 3):707-712 Wimalawansa SJ: Calcitonin gene -related peptide and its receptors: molecular genetics, physiology, pathophysiology, and therapeutic ... osteoblasts and osteoclasts [12,13] RANKL and OPG are members of a ligand -receptor system that directly regulates osteoclast differentiation and bone resorption, and both are produced and secreted...
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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" pot
Ngày tải lên :
20/06/2014, 07:20
... amylin, and calcitonin gene -related peptide on osteoclast development Bone 2001, 29:162-168 29 Chen FP, Hsu T, Hu CH, Wang WD, Wang KC, Teng LF: Expression of estrogen receptors alfa and beta mRNA and ... capsaicin and resiniferatoxin Biochem J 1995, 307(Pt 3):707-712 Wimalawansa SJ: Calcitonin gene -related peptide and its receptors: molecular genetics, physiology, pathophysiology, and therapeutic ... osteoblasts and osteoclasts [12,13] RANKL and OPG are members of a ligand -receptor system that directly regulates osteoclast differentiation and bone resorption, and both are produced and secreted...
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Redox regulation of estrogen receptor alpha and sodium hydrogen exchanger 1 gene expression by hydrogen peroxide
Ngày tải lên :
10/09/2015, 15:49
... 16 1.3 ESTROGEN RECEPTOR 17 1.3.1 Estrogen and estrogen receptors: Introduction 17 1.3.2 Estrogen receptor and genomic signaling 20 1.3.3 Membrane and cytoplasmic ... in benign and precancerous breast disease, suggesting that amplification of ERα gene is a common and early genetic alteration in proliferative breast disease and breast cancer Gene amplification ... through the production of ROS (Richard et al., 2000) 16 1.3 ESTROGEN RECEPTOR 1.3.1 Estrogen and estrogen receptors: Introduction Estrogens are steroid hormones produced primarily in the ovaries...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
Ngày tải lên :
16/02/2014, 15:20
... its role on the expression of reporter gene bearing an ARE in its 3¢ UTR We showed that overexpression of ASF ⁄ SF2 specifically down-regulated the expression of an ARE reporter gene Moreover, ... interacting candidates and were treated with arsenite (1 mM for 30 min) Cells were fixed and stained with mouse anti-HA and goat anti-TIAR sera Secondary Alexa 594-coupled donkey anti-mouse (red) and FITC-coupled ... between the nucleus and the cytoplasm Genes Dev 12, 55–66 ` 31 Gui JF, Tronchere H, Chandler SD & Fu XD (1994) Purification and characterization of a kinase specific for the serine- and arginine-rich...
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