... library of yeast chromosomal DNA It was found that 4.6 and 5.1 kb overlapping fragments of chromosome XIII carrying SNO1 and SNZ1 were capable of complementing the defect of mutant K64 (Figs and ... homologues SNO2, SNO3, SNZ2 and SNZ3 complement the defect The relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the ... maximum of 5% of that of the intact complex described above This suggests that although Sno1p and Snz1p associate spontaneously, renaturation of either one or both of the proteins was incomplete and...
Ngày tải lên: 19/02/2014, 12:20
... scenario, the equal error rate of our scheme is 7%, while that of the reference scheme is 20%; and the minimum total error rate (i.e sum of false positive rate and false negative rate) is at 14% for ... component lengths False positives of AMS and RPM (noiseless) False positives of AMS and RPM (noisy) False positives of AMS and RPM by distance False positives of RPM 58 60 ... packet features and possibly features of web objects Flow logs preserve features and statistics of flows and possibly object features, too Identifying subnet structures and key servers of an organization...
Ngày tải lên: 11/09/2015, 09:58
Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... system is capable of producing off-resonance, mirror, and flat magnetic field configurations A mirror ratio of 1·1 and a maximum flat field of 1400 G has been obtained The measured values and the design ... varying the size of the solenoid coils, thickness of iron jacket, and amp-turns (NI) The optimum values of NI for the side and middle solenoid coils were obtained to be 12950 and 8250 respectively ... ion source uses the principle of adiabatic invariance for mirror reflection, and high magnetic field mode of operation to successfully generate and confine plasma Design of the electromagnet The magnetic...
Ngày tải lên: 22/12/2013, 08:58
Báo cáo khoa học: "Implementing a Characterization of Genre for Automatic Genre Identification of Web Pages" pot
... upgrade of existing genres and the creation of novel genres The change of genre repertoire and the creation of new genres were well illustrated by Crowston and Williams (2000) and Shepherd and Watters ... can be generated from specific needs and requirements of the web Crowston and Williams (2000) have traced this evolution through a manual qualitative survey of 1000 web pages Shepherd and Watters ... probabilities of being argumentative_persuasive, 0.7 of being instructional and so on Probabilities are interpreted in terms of degree or gradation For example, a web page with 0.9 probabilities 702 of...
Ngày tải lên: 23/03/2014, 18:20
Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes" pptx
... Figure KSHV of a region12 the divergent locus B of a complete sequence CODEHOP strategy to determine themacaque homolog ofof CODEHOP strategy to determine the complete sequence of a region of the ... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... the utility of this CODEHOP PCR primer strategy by identifying and characterizing14 previously unknown DNA polymerase sequences from members of the alpha, beta and gamma subfamilies of herpesviruses...
Ngày tải lên: 18/06/2014, 22:20
IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE
... am so grateful to you for believing in me and letting me pursue my dreams The person I am today is because of you To my dear uncle and my aunt Khem Kandel and Laxmi Kandel: your support and friendship ... experiments The Argonne National Laboratory is operated by the University of Chicago Argonne, LLC, for the United States Department of Energy Office of Biological and Environmental Research under Contract ... structure of ALDH3A1 with CB7 .72 Crystal structure of ALDH3A1 with CB25 81 G Expression and Activity of ALDH3A1 and ALDH1A1 in Cancer Cell lines .86 H Sensitization of tumor cells...
Ngày tải lên: 24/08/2014, 09:51
the identification and characterization of proteins required for endocytosis in the budding yeast saccharomyces cerevisiae
Ngày tải lên: 14/11/2014, 06:20
Identification and characterization of CIS regulatory elements for vertebrate developmental control genes
... cis-regulatory elements through comparisons of promoters and 5` flanking regions of γ-globin and ε-globin gene of several primates and other mammals [58] Thus alignment of orthologous nucleotide sequences ... accommodative of all our demands Thanks to Department of Biological Sciences, National University of Singapore for funding my studies through the NUS research scholarship and for taking care of all ... The completion of a number of vertebrate genome sequences with its unprecedented coverage and resolution, as well as the concurrent development of genomic alignment, visualization, and analytical...
Ngày tải lên: 14/09/2015, 08:40
Identification and characterization of a novel heart reactive autoantibody in systemic lupus erythematosus possible serological marker for early myocardial dysfunction
... in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden of SLE ... humor, kindness, and help Thanks to all staff of Deparment of Rheumatology and Immunology, Singapore General Hospital for their help and friendship ii Thanks to all staff of Department of Clinical ... THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT First of all, I would like to...
Ngày tải lên: 14/09/2015, 12:42
Identification and characterization of IFI30 as a glioblastoma specific promoter for glioma gene therapy
... kidney, adrenal gland and spleen It was also shown that HSV-Tk caused depletion of the enteric glia and this resulted in severe inflammation and hemorrhagic necrosis of the jejunum and ileum (Bush ... promoter is that of the survivin gene Survivin is a member of the Inhibitor of Apoptosis (IAP) protein family and plays a vital role in the survival of cancer cells and the progression of malignancy ... tissues and is over expressed among a wide range of cancers including cancers of the oesophagus, pancreas, bladder, central nervous system, lung, prostrate gland, breast, uterus, melanoma and soft...
Ngày tải lên: 22/10/2015, 21:20
Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to avoid hemolysis and incubation ... beginning of the test and also at 0.25, 0.5, 1, 2, 8, 20, and 23 h intervals, one of the samples was harvested and then centrifuged at 3000 for One hundred μl of the supernatants were separated for ... efficiency and cell recovery) [25] Loaded amount The total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin...
Ngày tải lên: 25/10/2012, 11:10
Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... diameter of the PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane is 0.25 µm, 0.12 µm and 0.16 µm, respectively It can be seen that the polymerization of styrene and the blending of PS and ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 645 2.5 Measurement of proton conductivity The proton conductivity of the PVDF-SPS membrane and Nafion-117 ... compartment (mol.L-1) VB is the volume of right reservoir (cm3) A and L are the membrane cross-sectional area and thickness, respectively α is the slope of the linear plot of methanol concentration in...
Ngày tải lên: 05/09/2013, 16:11
Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
... cellulase of Coptotermes formosanus had Km and Vmax values of 1.90 mg/ml and 148.2 U/mg protein, respectively, on CMC [65] Nakamura et al [66] reported a Km of 3.3 mg/ml CMC and a Vmax of 1100 ... Assistant Professor and was promoted to tenured Professor in 2006 He earned his B.S in Mathematics and Agricultural Engineering in India, a M.S degree in Food Engineering in Thailand, and completed ... SD School of Mines and Technology (Rapid City, SD, USA), and SD State University (Bookings, SD, USA) He holds a Masters degree in Chemical Engineering and a PhD degree in Biotechnology and has...
Ngày tải lên: 05/09/2013, 16:11
Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... here concentrate on applications of COFRADIC in studying selected post-translational modifications ) protein processing [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for ... autosampler, and can be purchased from a variety of companies; and, at least in our hands, HPLC solvent gradients and flow rates can nowadays be controlled such that the overall reproducibility of HPLC ... altered and unaltered peptides to the highest degree) , the specificity and quantitative nature of the COFRADIC sorting reaction are clearly crucial for efficient peptide sort- COFRADIC and protein...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... and D; lane 1) and HEK293 (C and D; lane 2) cells The positions of the molecular size markers (kDa) are indicated to the left and right of the (C) and (D), respectively, whilst the position of ... the basal and PMA up-regulation of TPb expression and may provide a platform to determine the relative contributions of differentially regulated expression of TPa and TPb to haemostasis and possibly ... Research Board and Enterprise Ireland We are grateful to Dr Harinder Singh, Department Molecular Genetics and Cell Biology, University of Chicago for the gifts of pcDNA3:HaOct1 and pcDNA3:HaOct2...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... 000 d.p.m.) and nuclear extracts (5 lg protein) were mixed in 0.02 mL of 25 mM Tris/HCl (pH 7.9), 65 mM KCl, mM MgCl2, 0.25 mM EDTA and 10% glycerol in the presence of 400 ng of dI-dC, and incubated ... the 5¢-flanking region and determination of transcription start sites of mouse integrin a3 subunit gene The nucleotide sequence of the 0.5 kb SalI/SacI fragment and a part of exon is shown in Fig ... stretch of the sequence between the SalI and SacI sites upstream of exon 1, and that putative suppressor elements are present between the PstI (approximately 2.5 kb upstream of the SacI site) and...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx
... α-helix and β-sheet exhibit the high degree -of- fold, Q, as well as high Young’s modulus On the other hand, some protein materials such as titin Ig domains and TTR have the low degree -of- fold, ... high degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif For deeper understanding the role of native ... materials and degree -of- fold Q It is shown that degree -of- fold Q is highly correlated with Young’s modulus of protein materials Fig Relationship between maximum hydrostatic stress of protein...
Ngày tải lên: 22/02/2014, 08:20
Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
... of OsCCD1 for the C5¢–C6¢ and C7¢–C8¢ double bonds depends on the nature of the substrate The cleavage of a sole double bond in the cyclic moiety of 3-OH-c-carotene and of monocyclic b-apocarotenals ... represents a major volatile of tomato fruits [46] and roses [47] Moreover, citral, the mixture of geranial and its cis-isomer neral, is a major component of the aroma of lemon grass and other lemon-scented ... contained 40 lg of purified enzyme eluate at substrate concentrations of 80 lm (lycopene and 3-OHc-carotene) or 40 lm (zeaxanthin and synthetic substrates) For the production of lycopene micelles,...
Ngày tải lên: 07/03/2014, 03:20