ester made from methanol and formic acid
Tài liệu Báo cáo " Obtaining Essential Oil and Shikimic Acid from Star Anise Fruit (Illicium verum Hook) " ppt
... and shikimic acid from star anise fruits. With this method, obtaining yield of essential oil and shikimic acid respectively are 9.5% and 5.6% (essential oil content and shikimic acid content ... well as shikimic acid obtained by methods N0 1, N0 2 and N0 3 were shown at table 1 and table 2. Table 1. Obtaining simultaneously essential oil and shikimic acid from star anise fruits ... shikimic acid that it more simple and economic than Payne and Edmonds method. In this study, we used methanol : water (8:2) extraction to remove impurities from the crude shikimic acid, while...
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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
... and E. coli Rosetta(DE3)were purchased from Novagen (Madison, WI, USA).Restriction enzymes, modifying enzymes, and PCR enzymeswere from TaKaRa Bio (Kyoto, Japan). Crotalus atroxphosphodiesterase ... plates containing 50 lgÆ mL)1ampicillin and 30 lgÆmL)1chloramphenicol, and incubatedat 30 °C and 42 °C.Plasmid constructions, overproductions and purificationsPlasmids for overexpression ... PIPES as an internal standard, and 2 lgofprotein samples in a final volume of 100 lL. The reactionmixture was incubated for 10 min at 37 °C and transferredto 400 lL cold methanol. The contents...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... gum, and beechwood xylan were from Sigma (St. Louis,Mo.). Potato pectic galactan was from Megazyme Interna-tional (Bray, Ireland). Taq polymerase was from Gibco BRL(Breda, The Netherlands). ... agent.MaterialsD-Xylose,D-glucose,D-fructose,D-galactose,D-mannose, and lactose were obtained from Merck (Darmstadt,Germany).D-Glucuronic and D-galacturonic acid were from Fluka (Buchs, Switzerland). Mellibiose, raffinose,stachyose,L-arabinose, ... amino acid sequence of GALA revealed veryhigh similarity to the endogalactanases of A. tubingensis(95.7% amino acid sequence identity) and A. aculeatus(78.9% amino acid sequence identity) and...
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Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf
... GLCafter acid hydrolysis and was carried out for lamininpurified from starch-fed control (SFC) and starch-feddiabetic (SFD) groups as a representative.The results of composition analysis and GLCrevealed ... neutral sugars like mannose and galactose and amino sugar as N-acetylglucos-amine, along with sialic acid which accounted for 22 and 35% of total carbohydrate content in SFC and SFD groups, respectively.Purification ... dietary fiber and butyric acid were effective inbringing about changes in oligosaccharide moieties.Materials and methodsMaterialsStreptozotocin, PNGase-F, 2AA and Sepharose 4B were from Sigma-Aldrich...
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Báo cáo khoa học: Characterization of surface n -alkanes and fatty acids of the epiphytic lichen Xanthoria parietina, its photobiont a green alga Trebouxia sp., and its mycobiont, from the Jerusalem hills pot
... hexane-1,6-dioic acid, dimethyl ester; 2, tetradecanoic acid, methyl ester; 3, hexadecanoic acid, methyl ester; 4, octadecanoic acid, methyl ester; 5, dehydroabietic acid, methyl ester. Ó FEBS 2003 Alkanes and ... and octadecanoic acid (18 : 0), and also very long-chain n-alkanes C22–C34.Dehydroabietic acid was found in both lichen and mycobi-ont. The occurrence of different amounts of n-alkanes and fatty ... fatty acids: 9,12,15-18:3,9,12-18:2 ,and9 -18:1,aswellaslightn-alkanesC8–C15. The major saturated fatty acids obtained from themycobiont are: tetradecanoic (14 : 0), hexadecanoic (16 : 0)and...
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Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt
... humans.ResultsGlycans from seven urine samples from six galacto-sialidosis patients, five amniotic fluid samples from five mothers carrying a fetus suffering from galacto-sialidosis, and two ascitic ... 2.3U3, U4 Urine from patient MO (Lyon, France) NDU5 Urine from patient BO, 127 days old (Lyon, France) 1.2U6 Urine from patient B07 ⁄ 0175 (Amsterdam, The Netherlands) 1.6U7 Urine from patient ... (Deventer, The Netherlands).Acetonitrile was obtained from Biosolve (Valkenswaard,The Netherlands). All solutions were prepared using water from a Milli-Q synthesis system from Millipore BV(Amsterdam,...
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GENOTYPING OF CLOSTRIDIUM PERFRINGENS ISOLATED FROM CATTLE AND PIGS WITH DIARRHEA IN HANOI AND SURROUNDING AREAS, VIETNAM
... test result. Also, H2S production properties of isolates from cattle and pigs with and without diarrhea were 85.33%, 86.67%, 82.89%, and 66.67%, respectively. Table 2. Prevalence of C. perfringens ... C. perfringens isolates from diarrheic cattle, 57.34%, 41.33%, and 1.33% belonged to type A, type D, and type C, respectively; whereas all C. perfringens isolated from fecal samples of healthy ... isolates were originated from diarrheic cattle. The percentage of cpb2 and both cpe / cpb2 genes positive C. perfringens isolated from diarrheic pigs were 45.39% and 17.11%, respectively....
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Detection of bacteria and enteric viruses from river and estuarine sediment
... river from various contamination sources settle on river and estuarine sediment, and that the sediments may protect the bacteria from rapid decay as suggested by Gerba and McLeod (1976) and Anderson ... composition of silt and clay. The low recovery rate was also because of the inhibition of nucleic acids extraction and PCR by humic substances extracted from sediment by high pH as Tsai and Olson (1992), ... (Wait and Sobsey, 1983; Lewis et al., 1985). Moreover, it is reported that humic substances which are extracted from soil and sediment inhibit nucleic acids extraction (Zhou et al., 1996) and...
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