ester made from methanol and formic acid

Tài liệu Báo cáo " Obtaining Essential Oil and Shikimic Acid from Star Anise Fruit (Illicium verum Hook) " ppt

Tài liệu Báo cáo " Obtaining Essential Oil and Shikimic Acid from Star Anise Fruit (Illicium verum Hook) " ppt

... and shikimic acid from star anise fruits. With this method, obtaining yield of essential oil and shikimic acid respectively are 9.5% and 5.6% (essential oil content and shikimic acid content ... well as shikimic acid obtained by methods N 0 1, N 0 2 and N 0 3 were shown at table 1 and table 2. Table 1. Obtaining simultaneously essential oil and shikimic acid from star anise fruits ... shikimic acid that it more simple and economic than Payne and Edmonds method. In this study, we used methanol : water (8:2) extraction to remove impurities from the crude shikimic acid, while...

Ngày tải lên: 12/02/2014, 17:20

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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

... and E. coli Rosetta(DE3) were purchased from Novagen (Madison, WI, USA). Restriction enzymes, modifying enzymes, and PCR enzymes were from TaKaRa Bio (Kyoto, Japan). Crotalus atrox phosphodiesterase ... plates containing 50 lgÆ mL )1 ampicillin and 30 lgÆmL )1 chloramphenicol, and incubated at 30 °C and 42 °C. Plasmid constructions, overproductions and purifications Plasmids for overexpression ... PIPES as an internal standard, and 2 lgof protein samples in a final volume of 100 lL. The reaction mixture was incubated for 10 min at 37 °C and transferred to 400 lL cold methanol. The contents...

Ngày tải lên: 19/02/2014, 18:20

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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... gum, and beechwood xylan were from Sigma (St. Louis, Mo.). Potato pectic galactan was from Megazyme Interna- tional (Bray, Ireland). Taq polymerase was from Gibco BRL (Breda, The Netherlands). ... agent. Materials D -Xylose, D -glucose, D -fructose, D -galactose, D -mannose, and lactose were obtained from Merck (Darmstadt, Germany). D -Glucuronic and D -galacturonic acid were from Fluka (Buchs, Switzerland). Mellibiose, raffinose, stachyose, L -arabinose, ... amino acid sequence of GALA revealed very high similarity to the endogalactanases of A. tubingensis (95.7% amino acid sequence identity) and A. aculeatus (78.9% amino acid sequence identity) and...

Ngày tải lên: 21/02/2014, 01:21

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Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf

Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf

... GLC after acid hydrolysis and was carried out for laminin purified from starch-fed control (SFC) and starch-fed diabetic (SFD) groups as a representative. The results of composition analysis and GLC revealed ... neutral sugars like mannose and galactose and amino sugar as N-acetylglucos- amine, along with sialic acid which accounted for  22 and 35% of total carbohydrate content in SFC and SFD groups, respectively. Purification ... dietary fiber and butyric acid were effective in bringing about changes in oligosaccharide moieties. Materials and methods Materials Streptozotocin, PNGase-F, 2AA and Sepharose 4B were from Sigma-Aldrich...

Ngày tải lên: 22/03/2014, 17:20

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Báo cáo khoa học: Characterization of surface n -alkanes and fatty acids of the epiphytic lichen Xanthoria parietina, its photobiont a green alga Trebouxia sp., and its mycobiont, from the Jerusalem hills pot

Báo cáo khoa học: Characterization of surface n -alkanes and fatty acids of the epiphytic lichen Xanthoria parietina, its photobiont a green alga Trebouxia sp., and its mycobiont, from the Jerusalem hills pot

... hexane-1,6-dioic acid, dimethyl ester; 2, tetradecanoic acid, methyl ester; 3, hexadecanoic acid, methyl ester; 4, octadecanoic acid, methyl ester; 5, dehydroabietic acid, methyl ester. Ó FEBS 2003 Alkanes and ... and octadecanoic acid (18 : 0), and also very long-chain n-alkanes C 22 –C 34 . Dehydroabietic acid was found in both lichen and mycobi- ont. The occurrence of different amounts of n-alkanes and fatty ... fatty acids: 9,12,15- 18:3,9,12-18:2 ,and9 -18:1,aswellaslightn-alkanes C 8 –C 15 . The major saturated fatty acids obtained from the mycobiont are: tetradecanoic (14 : 0), hexadecanoic (16 : 0) and...

Ngày tải lên: 23/03/2014, 17:21

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Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt

Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt

... humans. Results Glycans from seven urine samples from six galacto- sialidosis patients, five amniotic fluid samples from five mothers carrying a fetus suffering from galacto- sialidosis, and two ascitic ... 2.3 U3, U4 Urine from patient MO (Lyon, France) ND U5 Urine from patient BO, 127 days old (Lyon, France) 1.2 U6 Urine from patient B07 ⁄ 0175 (Amsterdam, The Netherlands) 1.6 U7 Urine from patient ... (Deventer, The Netherlands). Acetonitrile was obtained from Biosolve (Valkenswaard, The Netherlands). All solutions were prepared using water from a Milli-Q synthesis system from Millipore BV (Amsterdam,...

Ngày tải lên: 29/03/2014, 09:20

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GENOTYPING OF CLOSTRIDIUM PERFRINGENS ISOLATED FROM CATTLE AND PIGS WITH DIARRHEA IN HANOI AND SURROUNDING AREAS, VIETNAM

GENOTYPING OF CLOSTRIDIUM PERFRINGENS ISOLATED FROM CATTLE AND PIGS WITH DIARRHEA IN HANOI AND SURROUNDING AREAS, VIETNAM

... test result. Also, H 2 S production properties of isolates from cattle and pigs with and without diarrhea were 85.33%, 86.67%, 82.89%, and 66.67%, respectively. Table 2. Prevalence of C. perfringens ... C. perfringens isolates from diarrheic cattle, 57.34%, 41.33%, and 1.33% belonged to type A, type D, and type C, respectively; whereas all C. perfringens isolated from fecal samples of healthy ... isolates were originated from diarrheic cattle. The percentage of cpb2 and both cpe / cpb2 genes positive C. perfringens isolated from diarrheic pigs were 45.39% and 17.11%, respectively....

Ngày tải lên: 29/08/2013, 08:14

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Detection of bacteria and enteric viruses from river and estuarine sediment

Detection of bacteria and enteric viruses from river and estuarine sediment

... river from various contamination sources settle on river and estuarine sediment, and that the sediments may protect the bacteria from rapid decay as suggested by Gerba and McLeod (1976) and Anderson ... composition of silt and clay. The low recovery rate was also because of the inhibition of nucleic acids extraction and PCR by humic substances extracted from sediment by high pH as Tsai and Olson (1992), ... (Wait and Sobsey, 1983; Lewis et al., 1985). Moreover, it is reported that humic substances which are extracted from soil and sediment inhibit nucleic acids extraction (Zhou et al., 1996) and...

Ngày tải lên: 05/09/2013, 10:15

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