establishment of immortalized human cell lines
Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
Ngày tải lên :
19/02/2014, 05:20
... profiles of HO-1 and HO-2 in eight human cell lines by northern and western blot analyses (Fig 1A,B) Expression of HO-1 5334 A B C Fig Expression profiles of HO-1 and HO-2 in various human cell lines ... expressed in all eight cell lines (Fig 1A,B) We also measured the heme content of these cell lines: it was about twofold higher in YN-1 and K562 erythroleukemia cells than in the other cell types (Fig ... expression levels of HO-1 Effects of HO-1 or HO-2 short interfering RNA (siRNA) on the expression of HO-1 Fig Effects of SA and SnPP on cellular heme content in human cell lines YN-1 erythroleukemia,...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
Ngày tải lên :
19/02/2014, 06:20
... expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of hypoxia ... certain human cell types under hypoxia Experimental procedures Cell culture and hypoxia study The human cell lines used were KG1 myeloid cells, K562 erythroid cells, and Jurkat T-lymphocyte cells ... expression levels of HO-2 mRNA and protein in five out of six cell lines, with the exception of KG1 myeloid cells A Hypoxia decreases the expression levels of HO-2 protein in YN-1 and K562 cells To confirm...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
Ngày tải lên :
16/03/2014, 06:20
... capacity of cells The human cancer cell lines chosen included one epidermoid carcinoma cell line, three mammary tumour cell lines and one human pancreas adenocarcinoma All five cell lines exhibited ... 4), as expected for a protein secreted as a result of the cellular production of cystatin C Cellular levels of cystatin C after incubation of the cells in medium containing lm cystatin C in a timescale ... 2008 FEBS 4573 Internalization of cystatin C U Ekstrom et al ¨ Fig Internalization of cystatin C in cancer cell lines measured by flow cytometry Five human cancer cell lines were used: MCF-7, MDAMB-453,...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
Ngày tải lên :
23/03/2014, 07:20
... cytotoxicity against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro ... (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) A 72 ... HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Ngày tải lên :
23/03/2014, 09:21
... propagated in 1% of initially transfected cells [4] EBV plasmid replication has been demonstrated for a large variety of human cell lines; primate and canine cell lines may also be used [5] It has been ... for full establishment of doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular backgrounds we use continuous expression of the ... bottleneck in stable transfection of cells arises from the low frequency of stable plasmid integration into genomic DNA At best, only 0.001% of cells generate clones Expansion of the few survivors takes...
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Báo cáo Y học: Conditionally immortalized adrenocortical cell lines at undifferentiated states exhibit inducible expression of glucocorticoid-synthesizing genes pot
Ngày tải lên :
31/03/2014, 15:20
... functionally undifferentiated cell layer analogous to that observed in rats Establishment of immortalized adrenocortical cell lines A number of cell lines were derived from primary cells prepared from ... induce detectable levels of P450aldo mRNA in the cell lines under current experimental conditions (data not shown) Steroidogenesis of the cell lines The ability of the cell lines to produce steroids ... precursor cell lines for the zona fasciculata cells The available evidence does not support the hypothesis that these cell lines represent bipotential progenitor cell lines or stem cell- like lines...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
Ngày tải lên :
15/02/2014, 04:20
... principal component for the cell lines (PC1, PC2, and PC3), two of the three AD cells lines were close, whereas the third cell line was close in PC2 to the other two cell lines, but separate in PC1 ... of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and compared to a normal control cell ... close LC cell lines and three SC cell lines were different in PC1, with a negative value for LC and a positive value for SC The 86-2 cell line (LC) was different in PC3 from the SC cell lines Subtypes...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
Ngày tải lên :
18/06/2014, 15:20
... of 12 0.051; out of 20 0.017; out of 59 0.062; out of 16 0.039; out of 25 0.165; out of Also available online at http://ccr.coriell.org/Sections/Support/Global/QCgenotype.aspx?PgId=412 Page of ... Location C Figure Human Recombinant GM-CSF Accelerate Migration of Fibroblasts deriving from Location C Full lines indicate initial wound area; dotted lines demarcate migrating front of cells GM – CSF ... to normal healthy fibroblasts Page of (page number not for citation purposes) Journal of Translational Medicine 2008, 6:75 and track cell lines through all of the operations necessary to establish...
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Báo cáo khoa học: " Radiosensitization of colorectal carcinoma cell lines by histone deacetylase inhibition" pdf
Ngày tải lên :
09/08/2014, 10:21
... development [15] Methods Cell lines and experimental treatments The origin of the human colorectal carcinoma cell lines is delineated previously [18] The HCT116 and SW620 cell lines were purchased ... that cell cycle arrest of breast carcinoma cell lines at the G2/M boundary comprises repression of the gene for Plk1, PLK [7-9] A variety of pharmacological compounds, designed to target cell ... nM) of the HCT116 and SW620 cell lines for a period of 0–24 hours resulted in cell cycle responses highly different from the irradiated phenotypes In the HCT116 cells, complete depletion of G1...
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Báo cáo y học: " Integrated analysis of breast cancer cell lines reveals unique signaling pathways" pot
Ngày tải lên :
14/08/2014, 21:20
... basal cell lines are far more invasive than the luminal cell lines [43] Clustering of the 'mixed' group of cell lines is strongly driven by the three Src modules (Figure 4) Src is one of the ... utility of symbolic systems modeling for the identification of key cell signaling events in the context of cancer Materials and methods Cell lines The complete panel contains 51 breast cancer cell lines ... expression across the panel of cell lines Proteins that showed a highly variable expression pattern across the panel of cell lines were considered present in some cell lines and absent from others...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
Ngày tải lên :
16/03/2014, 13:20
... clusters of G: GT-G1 has one cluster of four guanines, GT-G2 has two clusters of four guanines, GT-G3 has one cluster of seven guanines and one of four guanines The human T-lymphoblast CCRF-CEM cell ... complex (CRC) (B) UV crosslinking of G-rich GT oligomers Three micrograms of total nuclear extract of CCRF-CEM cells were incubated in buffer C in the presence of ng of the indicated 5¢-end 32P-labelled ... (lane 5) Discussion A series of guanosine-rich phosphodiester oligodeoxynucleotides strongly inhibits proliferation in a number of human tumour cell lines and the presence of FEBS Journal 273 (2006)...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... for measurements of intracellular fluorescence Dead cells were excluded by electronically gating data on the basis of forward- vs side-scatter profiles; a minimum of · 104 cells of interest were ... hepatoma-derived Hep1q0 cells [16] ROS When DCF signal was assessed as a direct index of ROS, all of the q0 cells had a reduced intracellular fluorescence compared to q+ cells Bone-derived q0 cells had the ... intracellular oxidants under standard growth conditions Yet, in the absence of glucose (stress conditions), intracellular levels of ROS in bone-derived q0 cells are 2.5 times those of muscle q0 cells...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
Ngày tải lên :
17/03/2014, 23:20
... per cell) , characteristic of leukemic or activated T cells HLA-DR was abundant on all cell lines (‡ · 105 copies per cell) Surface density of class I HLA was low on MT-1 cells (% · 104 per cell) , ... headgroups of glycosphingolipids occurring at high density in rafts All these properties may be characteristic of human T cell lines of leukemia/lymphoma origin, as a recent study in mouse cell lines ... HLA-DR molecules in the plasma membrane of human T cell lines of Ó FEBS 2002 Compartmentation of IL-2 receptor (Eur J Biochem 269) 1205 Fig Disruption of lipid rafts by cholesterol depletion...
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Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines ppt
Ngày tải lên :
28/03/2014, 14:20
... gynecological cancer cell lines Methods Cell culture All the cell lines used were described in Table [20-45] and obtained from Cell Lines Service (Eppelheim, Germany) Briefly, all cells were cultured ... in at least one of the cell lines For MMP-8, -12 and -21 only very weak mRNA expression could be observed in single cell lines Nine MMPs, which were present in most of the cell lines, were chosen ... the expression of the other MMPs in endometrial cancer cell lines According to our results, the Ishikawa cell line showed the broadest range of mRNA and protein expression of most of the MMPs analyzed...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
Ngày tải lên :
18/06/2014, 19:20
... subsets of MDSC have been identified that will help elucidate the role of these cells in the ontogeny, spread, and treatment of cancer Page of 20 Methods Cell Lines and Cell Culture Tumor cell lines ... characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines Journal of Translational ... expression of immune modulatory cytokines for groups of MDSC-inducing and non-inducing human cancer cell lines [16] These studies suggested multiple mechanisms of MDSC induction amongst tumor cell lines, ...
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Báo cáo khoa học: " Loratadine dysregulates cell cycle progression and enhances the effect of radiation in human tumor cell lines" pot
Ngày tải lên :
09/08/2014, 10:20
... the percentage of cells in the G2/M phase of the cell cycle Since Page of 12 the G2/M phase of the cell cycle is one of the most sensitive to radiation [17], this could explain some of the increased ... (arrow) Effect of Loratadine on In Vitro Cell Cycle Progression HT29 cells treated with loratadine (75 μM) for 24 hours Loratadine was then washed off and cells were irradiated (8 Gy) Cell cycle ... loratadinetreated cells In contrast to cells exposed to radiation alone, the loratadine-treated irradiated cells had returned to a more normal cell cycle distribution within 18 hours of the removal of loratadine...
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Báo cáo khoa học: " The membrane targeted apoptosis modulators erucylphosphocholine and erucylphosphohomocholine increase the radiation response of human glioblastoma cell lines in vitro" pdf
Ngày tải lên :
09/08/2014, 10:20
... sensitizes human malignant glioma cell lines to radiation-induced cell death ErPC sensitizes human malignant glioma cell lines to radiation-induced cell death Induction of apoptosis and clonogenic cell ... http://www.ro-journal.com/content/1/1/6 viable cells [%] 100 80 60 T98G A172 U87MG 40 20 24h 48h time 72h Figure cell induced death Human malignant glioma cell lines are resistant to radiationHuman malignant glioma cell lines are ... concentrationdependent cell death in all cell lines with T98G and A172 cells being more sensitive than U87MG cells at all time points (Fig 1C–E) Human malignant glioma cell lines are resistant to...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
Ngày tải lên :
09/08/2014, 23:20
... pluripotent stem cells However, less is known about the conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows ... the establishment of cellular differentiation programs To investigate the role of DNA methylation in these cellular programs, several studies have reported the comparisons of methylation profiles ... pairs of cell lines, and identified between 1.4 and 2% of the genome that is significantly differentially methylated at CG sites Of these regions, 6% are overlapping between the three cell lines...
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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt
Ngày tải lên :
10/08/2014, 05:21
... expression profile of immortalized hMSCs is similar with that of ESCs To gain insight into the convergence of 3A6 toward ESCs, we compared the expression profile of 3A6 with various normal human tissues, ... http://www.jbiomedsci.com/content/17/1/64 Page 11 of 13 Figure The expression profile of 3A6 immortalized hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic ... co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated beneath the layer of 3A6 cells (Figure 3C) Previously,...
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