establishment of a gene expression programme in the hindbrain

... 5'-gcccagctatgaactccttct-3' and 3'-tgaagaggtgagtggctgtct-5'; INDO: 5'-ggtcatggagatgtccgtaa-3' and 3'-accaatagagagaccaggaagaa-5'; GTSE1: 5'acgtgaacatggatgacccta-3' and 3'-gttcgggaaccggattattta-3'; ... CDC2: 5'-ggtcaagtggtagccatgaaa-3' and 3'-ccaggagggatagaatccaag-5'; and MKI67: 5'-ccccaaccaaaagaaagtctc-3' and 3'-gactaggagctggagggctta-5' Histopathology and immunohistochemistry on paraffinembedded ... initiation of adalimumab therapy Samples were loaded in triplicate, and results are the mean and standard error of the mean of gene expression, relative to the mean gene expression in a standard sample...

Ngày tải lên: 09/08/2014, 14:20

... Inflammatory pain Tissue injury initiates an inflammatory response that induces pain This type of pain is known as inflammatory pain Inflammatory pain is due mainly to the action of prostaglandins ... microarray-based approaches to examine gene expression and miRNA changes in the brainstem and prefrontal cortex in a mouse facial carrageenan injection model of orofacial pain At the brainstem level, increased ... et al., 2005) The ascending pain pathway involves main pathways: the lateral and the medial pain system (de Leeuw et al., 2005) The lateral pain system also known as the neospinothalamic tract...

Ngày tải lên: 10/09/2015, 08:34

... neuron in the sensory ganglion of cranial nerves VII, IX and X OROFACIAL PAIN The diagnosis and treatment of facial pain remains a great challenge for oral and maxillofacial surgeons The pain syndromes ... from the spinal trigeminal tract terminate in the caudal third of the spinal trigeminal nucleus (pars caudalis), convey general somatic afferent information from the face, oral cavity and dura mater ... trigeminal neuralgia, atypical facial pain, and temporomandibular joint pain (Claeys et al., 1992) Facial pain has many causes, including idiopathic factors, trigeminal neuralgia, dental problems,...

Ngày tải lên: 07/10/2015, 09:55

... separated into two groups (Fig 3b), one composed of aphidicolin-glycinate and floxuridine, and the other, of cyclocytidine and cytarabine (Ara C) The alkylating agents separated into several clusters ... a marker for clinical decisions regarding L-asparaginase therapy as well as a closer look at the use of Lasparaginase therapy for solid tumours Discussion We have described the pharmacological ... collected in parallel for RNA, DNA and protein For RNA, the interval from incubator to stabilization of the preparation was kept to

Ngày tải lên: 30/03/2014, 13:20

... CA, USA) Gene expression profiling Total RNA was extracted using Trizol reagent and the RNA quality was tested with the Agilent Bioanalyzer 2000 (Agilent Technologies, Santa Clara, CA) The RNA ... Baccini A: CCA: An R Package to Extend CanonicalCorrelation Analysis Journal of Statistical Software 2008, 23(12):1-14 Takakura S, Mitsutake N, Nakashima M, Namba H, Saenko VA, Rogounovitch TI, Nakazawa ... regulation of transcription, and small GTPase mediated signal transduction To visualize the functions of these miRNA targeted genes, a binary (red indicate participate in the functional category and...

Ngày tải lên: 18/06/2014, 15:20

... atypical AP treatment may increase levels of liver enzymes such as alanine aminotransfeaminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), and alkaline phosphotase ... and interpretation of data, drafting the manuscript JS, IL and JD acquisition of data, analysis and interpretation of data RY and MW interpretation of data, revisions of the manuscript Additional ... up-regulated and the other half are down-regulated in the nuclear protein category These genes include many transcription factors and DNA binding proteins that are critical for regulating a cascade of...

Ngày tải lên: 11/08/2014, 17:20

... nucleotides of the 5′ end of the genomic RNA (ATAAATG) and the genomic sequence around the 5′ termini of sgRNAs (underlined, CP [TTTTATAAA], p21 [TCTTAGAAA], p2 0A [ATTGAATGA], p20B [AAGTATATT]) were ... whereas that specific to p21 sgRNA was located on the stem of the SL structure and it was in the loop in the case of p20B sgRNA The integrity of these secondary structural features were maintained ... underlined) in (a) and by an arrow in (b), termination codon of the preceding ORF is underlined and marked with an asterisk and the translation initiation codon is in bold and marked with an arrow...

Ngày tải lên: 12/08/2014, 04:20

... 5'-GAAGCACTGAAGAATCTGAAGAG-3'; antisense: 5'-TCCAACACCAAGTAAGACCATC-3'), and βActin (sense: 5'-CGGCATTGTCACCAACTG-3'; antisense: 5'-CGCTCGGTCAGGATCTTC-3'), were synthesized by Genechem (China) ... and in the interpretation of data, and revised the manuscript critically Additional material Additional File Expression of GFP in AMs obtained from BALF (× 200) AMs obtained from BALF at days after ... carried out the experiments, participated in the experimental design and in the interpretation of data, and drafted the manuscript YC conceived of the study, participated in the analysis of data, helped...

Ngày tải lên: 12/08/2014, 14:20

... from the dataset were overlaid onto a global molecular network developed from information contained within the IPA database, and networks of genes in the dataset were then algorithmically generated ... and endothelial cells in a paracrine fashion Furthermore there is an increase in expression of "anti-inflammatory" cytokines which may be important in preventing an over-exuberant innate immune ... the number of significantly expressed genes they contain, based on a Pvalue that indicates the likelihood of the genes in a network being found together due to chance A score of indicates a in...

Ngày tải lên: 12/08/2014, 14:20

... few of them were not retained 35x 5a The size of the plant PG family and the patterns of PG duplication in Arabidopsis indicate that the PG family expanded in both Arabidopsis and rice after their ... regionsshowing a the RT-PCR metazoan, blocks inPGs in Matchingmetazoa, listfrom generated tags and blocks from bacteria,usedand a and plants.analysisfor alignment sorted for Figuretheir data matching patterns ... the RT-PCR analysis Additional data file is summary of expression tags including a list of the PG cDNAs from Arabidopsis and a list of the PG cognate ESTs from Arabidopsis Additional data file lists...

Ngày tải lên: 14/08/2014, 17:22

... produce inbred strains At the time of the study, 43 out of the 53 strains investigated had more than 40 generations of inbreeding The animals were bred in Pasteur's animal facility until weaning and ... conceived the experimental design, participated in the data analysis and interpretation, and contributed to the writing and editing of the manuscript All authors read and approved the final manuscript ... Additional data files The following additional data are available Additional data file is a table listing accession numbers for M spretus promoters obtained from GenBank Bank here data file Accession...

Ngày tải lên: 14/08/2014, 20:22

... library of canonical pathways that were most significant to the data set The significance of the association between the data set and the canonical pathway was measured in ways: 1) a ratio of the ... p-value determining the probability that the association between the genes in the dataset and the canonical pathway is explained by chance alone 28 2.7 Real-Time PCR Real-time PCR amplification ... by other factors including unfavorable anatomy The rabbit model is the one exception to this and has been successful in tPA therapy development leading to tPA as the standard of care in human...

Ngày tải lên: 02/10/2015, 17:13

... Molecular characterization of a 500 kDa bc1 sub-complex in the yeast deletion strains lacking Qcr9p, ISP or Bcs1p BN ⁄ PAGE analysis of a yeast mutant strain in which the gene encoding the Qcr9p ... PAGE analysis of the mitochondrial membranes isolated from all these deletion strains and from a wild-type strain In the DQCR9, DISP and DBCS1 strains, a protein band of approximately 500 kDa ... however, the interaction between the bc1 subunits was hypothesized only indirectly by assaying the steady-state levels of the remaining subunits in the mitochondrial membranes of yeast strains in which...

Ngày tải lên: 18/02/2014, 08:20

... levels of identity to sequences of 2-aminomuconate deaminases [6,8,27] or to any other sequences available in FASTA and BLAST database programs at the DNA Data Bank of Japan Recently, we reported the ... enzyme activity In contrast, the deaminase from strain 10d contained an FAD-like cofactor, similar to D-amino acid oxidases [25–27], as indicated by the absorption peak of the purified enzyme at 266 ... metacleavage pathway of 2-aminophenol in Pseudomonas sp strain AP-3 (A) Proposed pathway of 4-amino-3-hydroxybenzoic acid in Bordetella sp strain 10d (10) I, 4-amino-3hydroxybenzoic acid; II, 2-amino-5carboxymuconic...

Ngày tải lên: 19/02/2014, 16:20

... amino acids) [19], peptides consisting of amino acids 7–22 of parabutoporin (an a- helical part having the four amino acids LAKK identical to mastoparan) and of the first 28 amino acids of the opistoporins ... parabutoporin, this could explain the higher antibacterial activity on Gram-negative bacteria for parabutoporin compared to opistoporin Also with magainin analogs, an increase in antibacterial activity ... Gramnegative and Gram-positive bacteria and their activity was compared with the activity of melittin and mastoparan (Table 1) Parabutoporin inhibits the growth of all Gram-negative bacteria...

Ngày tải lên: 21/02/2014, 15:20

... Malacca The Straits of Singapore The Straits of Singapore The Straits of Malacca The South China Sea The South China Sea The Straits of Singapore The Straits of Malacca The South China Sea The Straits ... consists of a federation of 11 states in Peninsular Malaysia and the states of Sabah and Sarawak in the north of Kalimantan Kuala Lumpur, the national capital, Labuan UNEP/SCS – National Report Malaysia ... aim of the national report is to review existing information on the use of, and threats to, the Malaysian coastal and marine resources off the Straits of Malacca and the adjacent waters of the Andaman...

Ngày tải lên: 06/03/2014, 15:21

... methylated alditol acetates obtained in sugar- and methylation analyses correspond to the detector response of the GLC-MS Permethylation of dephosphorylated OS was performed in the same way as in the ... shown) The structures of the other Hep3-glycoforms were obtained in an analogous manner (data not shown) and for all glycoforms the hexoses were found to be members of a linear chain attached ... abundance was estimated from the area of molecular ion peak relative to the total area (expressed as percentage) Peaks representing less than 3% of the base peak were not included in the table Observed...

Ngày tải lên: 08/03/2014, 02:21

... Australian intelligence community ANAO Australian National Audit Office AQMI Al-Qaida pour le Maghreb Islamique [al Qaeda for the Islamic Maghreb] ASALA Armenian Secret Army for the Liberation of Armenia ... least because it was instrumental in creating an independent Catholic state out of the world’s largest Islamic polity; and the post-9/11 war against al Qaeda.3 At the same time, globalization and ... furthering the interests of another country at the expense of Australia’s interests.9 The end of the Cold War, the rise of al Qaeda, preparations for the 2000 Olympic Games in Sydney, the 9/11 attacks,...

Ngày tải lên: 15/03/2014, 21:20

... translation (see below), amyH was amplified using chromosomal DNA of H hispanica as template, and primers AmyH-T 7a (atatcatATGAATCGACCCCGAATTACC GGCAG) and AmyH-T7b (atataagcttGTCTCCGTGGCG TGCCAGCTTACTG), ... Quickchange mutagenesis were AmyKKfor (CCGGCAGTAAGCAGGCGTCTaagaaaACC GTTCTGAAAGGAATCG) and AmyKKrev (GGCCGTC ATTCGTCCGCAGAttctttTGGCAAGACTTTCCTTAGC) (bold letters indicate the nucleotides encoding the ... plus NADH as indicated Lane contains the translation reaction of preAmyH, reactions in lanes 2–4 were performed using preAmyH dialysed against a buffer containing 2.5 M KCl, and reactions in lanes...

Ngày tải lên: 23/03/2014, 06:20

... domains, and applied the result to the study of an interesting asymptotic behavior of the quadratic functions As a matter of fact, we reformulate 1.1 and related inequality in the spaces of generalized ... 2 Journal of Inequalities and Applications in the spaces of generalized functions Also, we obtain the general solution and prove the Hyers-Ulam stability of 1.1 in the spaces of generalized functions ... Hyers-Ulam stability of the functional equations that have the quadratic property,” Journal of Mathematical Analysis and Applications, vol 222, no 1, pp 126–137, 1998 17 L Hormander, The Analysis of...

Ngày tải lên: 22/06/2014, 02:20