essential diagnostic and therapeutic considerations

... integrins, and VE-cadherin, and the angiostatic factors are angiostatin, endosatstin and thrombospondin (Distler 2003) Angiogenic factors such as angiopoietins and (Ang and Ang 2), and VEGF orchestrate ... which has no known ligand, may form heterodimer with EGFR in response to ligands including EGF and TGF-α and that this heterodimer is essential during cardiovascular development and angiogenesis (Camenisch ... ligand, is found on a number of cells including EC, macrophages, B-cells and vascular SMC The interaction between CD40 and its ligand causes severe inflammatory responses, matrix degradation and...

Ngày tải lên: 27/06/2014, 17:20

... BKD, HAG, NW, WS, FG and RTS conceived and designed the study BKD, DK, CK, and TC performed experiments BKD, DK, RS, HAG, NW, WS, FG and RTS analyzed and interpreted data JH, IR and WMK, were involved ... the right ventricular wall (RV) and that of the free left ventricular wall and ventricular septum (LV+S) Histology and Pulmonary Vascular Morphometry Lung histology and vascular morphometry were ... septum weight ratio (RV/(LV+S)) and (C) systemic arterial pressure (SAP) are shown Double immunostaining for von Willebrand factor and a-smooth muscle actin, and elastica staining were performed...

Ngày tải lên: 12/08/2014, 13:22

... Committee and the King Faisal Specialist Hospital and Research Centre’s Research Advisory Council Results Of the 24 consecutive neonates treated with both HFOV and INO, 18 (75%) responded and (25%) ... HFOV and INO in reducing the need for ECMO has been studied Kinsella et al [8] combined HFOV and INO in the treatment of infants with hypoxic respiratory failure and PPHN who were ECMO candidates ... the responders, and the remaining responded gradually during the INO treatment However, OI and a/A ratio remained almost the same throughout the treatment in the non-responders and the non-survivors...

Ngày tải lên: 12/08/2014, 18:20

... experiments performed by Foitzik and colleagues [39] and Wang and colleagues [40], as well as with clinical findings published by Beger and colleagues [24] and Ammori and colleagues [41], who found ... < 0.001) and mesenteric lymph nodes of the small bowel (p = 0.010) and colon (p = 0.001) In contrast, therapeutic antibiotic treatment on-demand only reduced pancreatic superinfection and infection ... WH, RL and JW played a pivotal role in planning and carrying out the experiments KW-S and MK performed bacterial and mycological analysis of the experimental animals SF, WH, TH, LS, MB and JW...

Ngày tải lên: 13/08/2014, 11:23

... mg/kg) and hexamethonium bromide (20 mg/kg; SigmaAldrich NV/SA, Bornem, Belgium) Haemodynamic and blood gas measurements were performed at the following time points: baseline, and 1, 5, 10 and 30 ... Materials and methods This investigation conforms to the Guide for the Care and Use of Laboratory Animals, published by the US National Institutes of Health (Publication No 85-23, revised 1996) [17] and ... sufentanil (3 μg/kg per hour) and pancuronium (0.2 mg/kg per hour) Mechanical ventilation with a mixture of oxygen and room air was adjusted to achieve normocapnia and normoxia, as controlled with...

Ngày tải lên: 13/08/2014, 11:22

Ngày tải lên: 19/02/2014, 00:20

Ngày tải lên: 09/03/2014, 12:20

... to 0.156 and generally had fairly large standard deviations (ranging from 0.250 to 0.532) Table shows the results for the mean level and random effects main effects only models (models and 2) For ... for the mean level model and 38% for the random effects models, respectively) and both models resulting in two predictive errors greater than 0.10 In both mean and random effects models the predictions ... functioning and QoL The content of the measure was derived directly from PH patients and all scales have been shown to have good face and content validity, reliability, reproducibility and construct...

Ngày tải lên: 18/06/2014, 19:20

... Dysfunction of cellular components of the arterial wall and of inflammatory and haemostatic systems are interrelated predicted survivals of 69% and 57%, respectively Although abnormal liver function ... contrast, the prostanoids improve function and survival in PAH, and may also be antiproliferative In the healthy endothelium, prostacyclin exerts vasodilatory and antithrombotic properties Epoprostenol, ... proliferation, reduced collagen deposition and inhibition of NF-ΚB activation [5,12,13] Since there are commonalities in the pathophysiology of PAH and RP, particularly in the context of connective...

Ngày tải lên: 09/08/2014, 06:23

... TW, YJC, GMH, JA and XRH carried out the RT-PCR, Western blot, and enzymatic activity assays XHZ and FQW participated in the conception, design and coordination of the studies and critically reviewed ... hypertensive rats J Clin Invest 2001, 107:703-15 Borland JA, Kelsall C, Yacoub MH, Chester AH: Expression, localisation and function of ACE and chymase in normal and atherosclerotic human coronary arteries ... minced and heated in 0.1 M HCl at 100°C for 10 min, then homogenized After centrifugation at 15,000 g for 30 min, the supernatant was lyophilized and redissolved in 400 μl assay buffer, and the...

Ngày tải lên: 12/08/2014, 11:21

... histopathological analysis and cultured the PASMCs MX and HYD carried out the RT-PCR and Western blotting assays MQD, PTZ, WN and MLL participated in directing the experimental techniques and coordination ... expression of p27kip1 and Skp-2 in rat lung tissue and cultured PASMCs Aiming to know whether p27 kip1 and Skp-2 were involved in chronic hypoxia-induced pulmonary hypertension and artery remodeling, ... of p27kip1 and Skp-2 in rat lung tissue and cultured PASMCs To further investigate whether p27kip1 and Skp-2 were regulated at transcriptional level, the mRNA levels of p27kip1, Skp-2 and b-actin...

Ngày tải lên: 12/08/2014, 13:22

... software on LSR II and analyzed with FlowJo v8.8.6 Measurement of right ventricular hypertrophy The ventricles and septum of the animals were collected and the wet and dry ventricle and septal weight ... exposure and femurs and tibias of the rats were dissected sterilely After cutting each end of the femurs and tibias to expose marrow, we placed each bone into a 1.5 ml sterile eppendorf tube and centrifuged ... Resource and Research Center at University of Missouri (Columbia, MO) and used as bone marrow cell donors CXCR4 inhibitor and hypoxic pulmonary hypertension Rats were placed in a hypoxia chamber and...

Ngày tải lên: 12/08/2014, 13:22

... TNF-a and IL-6 (Figure 3) Hydrogen-rich saline treatment decreased MDA and 8OHdG concentrations and increased SOD activity in serum and lung tissues Concentrations of MDA and 8-OHdG in serum and ... 8-OHdG and MDA levels, and SOD activity in serum and lung tissue Hydrogen-rich saline treatment (5 ml/kg once daily for or weeks) significantly decreased the 8-OHdG (A and B) and MDA (C and D) ... histological analyses, and helped to draft the manuscript XJS and XMZ conceived and designed and coordinated the study, analyzed the data, and wrote the manuscript JJH performed the analyses and participated...

Ngày tải lên: 12/08/2014, 13:22

... from (D and G) sham-operated animals, (E and H) BLEO (bleomycin-treated mice), and (F and I) AM treated animals show that abundant extracellular matrix (ECM) deposition, alveolar thickening, and ... RDP, MG and EM designed methods and experiments, carried out the laboratory experiments, analyzed the data, interpreted the results and wrote the paper ET and VM co-designed the dispersal and colonization ... controlled environment and provided with standard rodent chow and water Animal care was in compliance with Italian regulations on protection of animals used for experimental and other scientific...

Ngày tải lên: 12/08/2014, 13:22

... Research and Development, Sandwich, UK Authors’ contributions DK, HAG, NW, WS, FG and RTS conceived and designed the study DK, BK and HL performed experiments DK, BK, HL, BKD, HAG, NW, FG, WS and ... NW, FG, WS and RTS analyzed and interpreted data AS and TC were involved in interpretation of data DK and RTS drafted and finalized the manuscript BKD, AS, TC, WJ, RPB and ND were involved in revising ... echocardiographic (echo) measurements (Figure 4) RV/(LV+S) and RVWT-echo (r = 0.88, P < 0.0001); RVSP and RVWT-echo (r = 0.81, P < 0.0001) and RVWT invasive and RVWT-echo (r = 0.81, P < 0.0001) showed the...

Ngày tải lên: 12/08/2014, 13:22

... (desmin and a-SMA) [12] MFLCs were Factor VIII and desmin negative and vimentin and a-SMA positive [12] Pulmonary arterial fibroblast-like cells were Factor VIII, desmin and a-SMA negative, and vimentin ... von Willebrand factor (Factor VIII) andaSMA ELCs were positively stained for the endothelial cell (EC)-specific marker (Factor VIII) and the mesenchymal-specific marker (vimentin) and negative ... anti-von Willebrand factor, anti-vimentin and anti-desmin) for hour at room temperature, and then with secondary antibodies (antimouse IgG conjugated with Alexa-594 fluorescent dye and anti-rabbit...

Ngày tải lên: 12/08/2014, 14:20

... VCAM-1, ET-1 and MCP-1 mRNAs in IL-6-/- and IL-6+/+ during normoxic and hypoxic conditions Lung expression of ICAM-1, VCAM-1, ET-1 and MCP-1 mRNAs in IL-6-/- and IL-6+/+ during normoxic and hypoxic ... mix (10 nmol/L), and 100 ng random primers in a total volume of 12 μL was incubated for minutes at 65°C and chilled on ice Then, μL of 1st Strand Buffer, μL of DTT (0.1 mol/ L), and 40 U of ribonuclease ... IL-6+/+ and IL-6-/Hemodynamic response to acute hypoxia in IL-6+/+ and IL-6-/- mice Individual and mean (horizontal line) right ventricular systolic pressures (RSVP) in normoxic IL-6+/+ and IL-6-/-...

Ngày tải lên: 12/08/2014, 14:20

... http://respiratory-research.com/content/10/1/110 Figure B) eNOS, (C and D) iNOS, (E and hypertension (A, C, E) and from control patients of similar age (B, D, F) stained for (A and Lungs from infants with pulmonary F) ... infants with pulmonary hypertension (A, C, E) and from control patients of similar age (B, D, F) stained for (A and B) eNOS, (C and D) iNOS, (E and F) nNOS (A) Cardiac patient small pulmonary ... iNOS and (C) nNOS in lungs from infants with pulmonary hypertension and from conIntensity of staining for (A) eNOS, (B) iNOS and (C) nNOS in lungs from infants with pulmonary hypertension and...

Ngày tải lên: 12/08/2014, 14:20

... scleroderma model and nasal tolerance and the immunofluorescence experiments, and drafted the manuscript; WRT contributed to conception and design of the original study and analysis and interpretation ... (A, B and C, respectively) Panels G, H and I show the differential gene expression of the mRNA for collagens I, III and V, respectively, in lung tissue of control and immunized animals and after ... histological, immunohistochemical and molecular data; DMA and ERP contributed to the acquisition of histologic and morphometric data and performed the statistical analysis; RK, CCO and MHK contributed to...

Ngày tải lên: 12/08/2014, 14:20