esrom function in the growth cone
Commissural axon pathfinding at intermediate targets in the zebrafish forebrain
Ngày tải lên :
14/09/2015, 10:34
... about the extracellular environment into signaling networks within the growth cone These networks in turn regulate the cytoskeletal and adhesive dynamics of the growth cone, translating the extracellular ... afferents in the zebrafish embryo By lipophilic dye tracing, we find that axons innervating the habenula derive primarily from a region in the lateral diencephalon containing calretinin-expressing ... originating in the forebrain In other species, midbrain neurons have been shown to innervate the habenula via the fasciculus retroflexus (Stacker et al., 1993) The presence of such ascending...
- 120
- 286
- 0
Identification and characterization of proteins that interact with zonula occludens proteins
Ngày tải lên :
16/09/2015, 08:31
... carboxylate-binding loop The last four residues in the C-terminus of the peptide ligand interact directly with the peptide-binding groove The main chain atoms of the βB strand form hydrogen bonds with the ... domains are 50-70 amino acid domains that bind to PXXP sequences in other proteins More recently, the SH3 domain in MAGUKs has been shown to interact with the guanylate kinase (GK) domains The ... function Several lines of evidence contributed to this line of thinking Immunostaining with antibodies against occludin in chick intestinal epithelia showed that it exclusively localized to the...
- 223
- 378
- 0
Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
Ngày tải lên :
18/02/2014, 06:20
... exclusively in the pollen Siliques were stained at the base and at the tip, with overall very weak staining of the fused carpels In the root, the central cylinder was stained, whereas the primary ... proRAP2.4:GUS lines showed GUS expression in 3-dayold seedlings in cotyledons and in the central cylinder of the root, but not in the lower parts of the hypocotyl (a) Rosette leaves were stained in the ... RAP2.4 in planta, and also that CUL3a can assemble with other proteins either in the nucleus or the cytoplasm Discussion This work provides novel information, including the first description linking...
- 12
- 657
- 0
Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
Ngày tải lên :
19/02/2014, 06:20
... position of the more intensively oxidized polypeptide in the assay The bracket underlines the domain (molecular mass range 10–40 kDa) of the oxyblots that contains the greatest changes in protein oxidation ... of the initiator ATG in exon (Ex1) of the 67 exon-containing htt gene [5] Pathogenesis in HD correlates with the cleavage of mutated htt and the release of an N-terminal fragment bearing the ... performed to analyze the EGFP-containing inclusions Figure 1F shows that the expression of Hsp70 together with Hdj-1 (Hsp40) did decrease the average size of the EGFP-containing inclusion bodies...
- 18
- 721
- 0
Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... (p0), the peptide (0.1–2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the subphase was monitored for the ... the permeability of the IM of E coli, · 106 cells were mixed with lm SYTOX Green in NaCl ⁄ Pi for in the dark After addition of peptide, the increase in fluorescence, owing to the binding of the ... erythromycin, the antibiotic that gives the best synergy with the peptide, as indicated by the lowest FIC (Table 2) Erythromycin is a hydrophobic molecule that inhibits protein synthesis by blocking...
- 18
- 494
- 0
Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
Ngày tải lên :
23/03/2014, 13:20
... of proteinase K, indicating that they are indeed formed by the interaction of proteins and DNA (data not shown) UV cross-linking assays were carried out to estimate the size of the proteins responsible ... was used In lane 1, the reaction was done in the absence of proteins In subsequent lanes, the reactions were done with 75 mM fraction as the protein source and in the presence of increasing concentration ... all proteins present in the loaded protein fraction were recovered in the column flow-through, indicating that the proteins eluted in the affinity columns associated specifically with the telomeric...
- 14
- 469
- 0
Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
Ngày tải lên :
20/02/2014, 01:20
... Nop53p interacted in the two-hybrid system independently of the tag, but the interaction was stronger when Nop17p was fused to the DNA binding domain (BD-Nop17p; Fig 1) Further protein interaction ... function in the conversion of uridine into pseudouridine [17–23] In addition to the core snoRNP proteins, other proteins have been found to be associated with the snoRNPs and to participate in ... test the interaction of Nop53p with other proteins (A) Test for positive interactions between Nop53p and other proteins fused to the Gal4p activation domain (AD), or to the lexA DNA binding domain...
- 14
- 505
- 0
Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
Ngày tải lên :
08/03/2014, 08:20
... abundant in the fractions containing highest NR/ GS inhibitory activity (Fig 2) The NR/GS inhibitor behaved on Superose 12 gel filtration as a 55-kDa protein (not shown) The intact proteins and ... spinach leaves [6], or cauliflower (Fig 1) In contrast to 14-3-3 proteins, the ÔinterferingÕ protein inhibited both phosphorylated and dephosphorylated NR (Fig and data not shown) A protein inhibitor ... Similar to NRI, we found that our interfering protein bound to Con-A (Fig 2) The inhibition of NR by the interfering protein could be prevented by addition of either EDTA, ATP, or AMP directly...
- 7
- 457
- 0
Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
Ngày tải lên :
15/03/2014, 00:20
... from the X-ray structure of the C-terminal domain of Ure2p and integrates the finding that the N-terminal domain of Ure2p is flexible An enlargement of the region of Ure2p involved in the interaction ... comes from the identification of cross-linked peptides [16–23] The interfaces involved in protein–protein interactions can be also identified from the changes in the intensity of monolinked peptides, ... cross-linking approaches allow: (a) the identification of surface areas involved in protein-protein interactions within protein complexes; (b) the characterization of the distance constraints within...
- 12
- 510
- 0
Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx
Ngày tải lên :
16/03/2014, 06:20
... and their associated proteins are conserved from yeast to humans, and function in the sorting of ubiquitinated cargoes into intraluminal vesicles that are generated by inward budding of the endosomal ... evolution of the penta-EF-hand Ca2+-binding proteins Biochim Biophys Acta 1600, 51–60 Maki M & Shibata H (2007) The penta-EF-hand protein ALG-2 and its interacting ptoteins Calcium Binding Proteins 2, ... 20 min, quenched in 50 mm NH4Cl in NaCl ⁄ Pi for 15 min, and permeabilized in 0.1% (w ⁄ v) Triton X-100 in NaCl ⁄ Pi for After blocking with 0.1% (w ⁄ v) gelatin in NaCl ⁄ Pi for 30 min, the...
- 11
- 412
- 0
Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx
Ngày tải lên :
16/03/2014, 11:20
... peptide of nine amino acid residues, DWEDRYYRE, of the murine ⁄ rat prion protein (amino acids 144–152) was incorporated into an immunogenic surface loop of the L1 major capsid protein of bovine papillomavirus ... grafting or blood transfusions has raised further concern [4,5] The exact pathogenetic mechanism of prion diseases has remained uncertain In the most accepted ‘proteinonly’ hypothesis, the causative ... proteins To determine whether PrP–VLPs retain, at least in part, the antigenic surface structures of wild-type L1–VLPs, the immunoreactivity of intact particles was examined by ELISA, using a...
- 12
- 322
- 0
Báo cáo khoa học: The Rab5 effector Rabaptin-5 and its isoform Rabaptin-5d differ in their ability to interact with the small GTPase Rab4 doc
Ngày tải lên :
16/03/2014, 18:20
... pull-down experiments Interestingly, both the deletion found in the Rabaptin-5d polypeptide chain and the Rab4 binding site of Rabaptin-5 are located in the N-terminal portion of protein However, some ... discrepancy exists in the literature concerning the position of the Rab4-binding site While Vitale et al mapped it between amino acids and 135, by analyzing protein–protein interactions in yeast two-hybrid ... Rabaptin-5d corresponding to amino acids 140–294 of Rabaptin-5, which contains the deleted region, was unable to bind Rab4 In addition, the first 251 amino acids of the Rabaptin-5c isoform, bearing...
- 10
- 411
- 0
Báo cáo Y học: The inhibitory region of troponin-I alters the ability of F-actin to interact with different segments of myosin pot
Ngày tải lên :
17/03/2014, 10:20
... to in uence the surface activity of actin towards other actin binding proteins The site of binding of the inhibitory region is the N-terminal of actin that is structurally linked to the C-terminal ... tropomyosin on the inhibitory region of TnI The binding affinity of the myosin loop peptide hcM398–414 to F-actin and F-actin–tropomyosin was initially determined from the changes in intrinsic tryptophan ... how the actin-binding properties of peptides representing regions of the myosin molecule are affected by the interaction of actin with peptides incorporating the inhibitory domain of TnI The...
- 13
- 524
- 0
Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
Ngày tải lên :
17/03/2014, 23:20
... CGATCGGGGCGGGGCGAGC-3¢ (underlined bases denote the functional core of the MREs and the Sp1 binding element, and the mutated bases are indicated by italic type) Western blot analysis The MREa-binding proteins purified ... determine whether the MREa– protein complex displays sequence specificity Binding of the HepG2 nuclear proteins to MREa was inhibited by the addition of a molar excess of unlabelled MREa to the binding ... another band of 82 kDa when the MREa-protein band from the EMSA was excised from the native gel and resolved by SDS/PAGE (unpublished data) We purified the MREa-binding proteins using the avidin–biotin...
- 11
- 628
- 0
Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
Ngày tải lên :
18/03/2014, 01:20
... black shading; gray shading shows identity The number of residues corresponds to that of ablomin The underline in ablomin, tri in, latisemin, and tigrin shows the amino-acid residues determined by ... nonreducing conditions) (C) Primary structure of tigrin The residues determined by peptide sequencing are underlined We examined the effects of ablomin and tigrin on high K+- or caffeine-induced ... measuring the effect of the proteins, all the H-T solutions contained the indicated concentrations of proteins RESULTS Identification, isolation and cloning of tigrin and ablomin During the isolation process...
- 8
- 472
- 0
Báo cáo khoa học: Polyamines interact with DNA as molecular aggregates Luciano D’Agostino1 and Aldo Di Luccia doc
Ngày tải lên :
23/03/2014, 21:21
... arrangement of polyamines In Fig 3, the NMR-spectra of the single polyamines (putrescine, spermidine and spermine) dissolved in D2O (A) or in D2O phosphate buffer (B) are shown The Fig In vitro aggregation ... production of the 4800 NAP in the h following gastrin treatment These data indicate that the rapid formation of 4800 NAP occurs only in conditions of efficient polyamine metabolism assuring the maintenance ... phenomenon The NAPs are rich in spermine and spermidine The appearance of these two polyamines in the nucleus is crucially important for the induction of cell mitosis [1–4] Because of their high...
- 9
- 282
- 0
Báo cáo sinh học: " Soilborne wheat mosaic virus (SBWMV) 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing" docx
Ngày tải lên :
18/06/2014, 22:20
... a mutation in the TBSV P19 ORF eliminating expression of a protein that suppresses RNA silencing [10,42] The plasmid pTBSV.GFP contains GFP inserted into the TBSV genome replacing the viral CP ... Since there are viruses not included in the Pfam report that encode CRPs, this study was undertaken to determine if there is a larger CRP family containing related viral proteins Further examination ... [25] The SBWMV 19K protein is a CRP because it contains nine Cys residues [20] Seven of these Cys residues are conserved in all furovirus proteins and are located in the Nterminal half of the...
- 11
- 356
- 0
Báo cáo hóa học: " The ability of flagellum-specific Proteus vulgaris bacteriophage PV22 to interact with Campylobacter jejuni flagella in culture" pptx
Ngày tải lên :
20/06/2014, 01:20
... infected Proteus vulgaris were identified from drainage water samples in the Moscow region Bacteriophages that infect P vulgaris, as in the case of other bacteria, have been utilized for typing ... cells was determined to be × 10-9 ml/min Phage PV22 did not produce lytic cells in medium containing C jejuni strains At the same time, it was demonstrated by a spot test that the growth of C jejuni ... co-principle investigator for funding with collaborator B S Seal at the Poultry Microbiological Safety Research Unit, ARS, USDA in Athens, GA, USA who completed writing and final editing of the manuscript...
- 5
- 260
- 0
Báo cáo sinh học: " Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production" doc
Ngày tải lên :
06/08/2014, 18:20
... on the same blots, to allow estimation of the fraction of the intracellular proteins that were recovered in the virions To examine the potential role of the amino terminus of endophilin in incorporation, ... between the matrix and capsid domains [15-17] In lentiviruses, the L domains have distinct motifs - PTAPP in HIV-1 [18,19] and YXXL (in the single-letter amino-acid code where X is any amino acid) in ... Endophilin 237 306 V 368 SH3 ∆SH3 V+SH3 N125 N156 ++++ + +/− − Identifying binding domains in endophilin for Mo-MuLV Gag using the yeast two-hybrid system To determine the region in endophilin sufficient...
- 17
- 225
- 0