equipment test methods and raw materials

... PB-Nr: S05 Supply and stock of raw materials flow/process documentation/competence Issed by Hr Bauer Date of release Version 00 Page 2/5 PB-Nr: S05 Supply and stock of raw materials Supplier ... release Version 00 Page 4/5 PB-Nr: S05 Supply and stock of raw materials Applicable documents Factory regulations Handling dangerous materials Filling raw material tanks R03.01 - stocks regulations ... business with the purchase Mark With raw materials to the easier discoverableness on the bundles or the pallet by means of spraying colour, marker the internal raw material designation one applies...

Ngày tải lên: 25/01/2014, 00:20

... fibres: Part 1-40: Measurement methods and test procedures Attenuation Part 1-41: Measurement methods and test procedures Bandwidth Part 1-42: Measurement methods and test procedures Chromatic ... 1-43: Measurement methods and test procedures Numerical aperture Part 1-44: Measurement methods and test procedures Cut-off wavelength Part 1-45: Measurement methods and test procedures Mode ... Measurement methods and test procedures Monitoring of changes in optical transmittance Part 1-47: Measurement methods and test procedures Macrobending loss Part 1-48: Measurement methods and test...

Ngày tải lên: 04/04/2014, 11:52

... Kawatta, Mayorca, Rubi and Westin; Pineapple and Tarocco A and Oliverlands, Cadenera and Homosassa, respectively; while Torregrosa, Jaffa, Biondo, Finike, Sanguinea, Moro and Early Oblong are the ... packers, Food Industrial Processes – Methods and Equipment shippers, wholesalers, retailers and consumers frequently have different perspectives regarding to quality and often place different emphasis ... etc.) and since they change during handling research data can give us information on the waya product should be handled postharvest Inherently, the demand of fruit quality, physically and chemically...

Ngày tải lên: 28/06/2014, 09:20

... research topics in raw materials, processes and mechanical and other properties of conventional and advanced ceramic materials, included in this volume, are: The evaluation of local raw materials for ... gases and nanoscale oxides and the evaluation of perovskite structures ceramics for sensors and ultrasonic applications Section II includes six topics in raw materials, processes and mechanical and ... tensile and shear strength, fracture toughness and ductility, hardness and abrasion resistance and elasticity/plasticity Mechanical properties are important in structural and building ceramics, and...

Ngày tải lên: 29/06/2014, 12:20

... within one 5°C increment for Methods C and D and there should be no measured difference for Methods E and F The reproducibility for methods D and E is three 5°C increments, and for Method F it is two ... test period The standard test period is 24 h This test period was evaluated by interlaboratory testing, 16.1.2 The test period must be reported as indicated in 15.1 12.1.4 At the end of the test ... Test Program Results for ASTM G48 Standard Test Method for Pitting and Crevice Corrosion Resistance of Stainless Steels and Related Alloys by Use of the Ferric Chloride Solution - Methods E and...

Ngày tải lên: 13/01/2016, 17:51

... a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA) All these techniques have advantages and disadvantages Solution methods ... remaining tissue From: Methods in Molecular Biology, Vol 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A Corfield © Humana Press Inc., Totowa, NJ Davies and Carlstedt To isolate...

Ngày tải lên: 08/10/2012, 10:25

... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide...

Ngày tải lên: 08/10/2012, 10:25

... core protein specificity), and lower sensitivity than slot-blot and immunoassay methods Chap- Detection and Quantitation of Mucins 47 ter (2) discusses these assays and they are mentioned here ... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... mucins, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ Carraway, K L (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins...

Ngày tải lên: 08/10/2012, 10:25

... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4) This is a rapid and simple method for ... significance by methods that compare nonparametric distributions such as the Mann-Whitney U -test Values for thickness should therefore be expressed in terms of medians and upper and lower quartiles...

Ngày tải lên: 08/10/2012, 10:25

... Einerhand, A W C., and Dekker, J (1995) Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3, and more Biochem Soc Trans 23, 814–818 11 Van Klinken, B J W., Einerhand, A W C., Büller, H A., and Dekker, ... PhosphorImager The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references (1,4–11) The precursor ... Strous, G J and Dekker, J (1992) Mucin-type glycoproteins Crit Rev Biochem Mol Biol 27, 57–92 Van Klinken, B J W., Dekker, J., Büller, H A., and Einerhand, A W C (1995) Mucin gene structure and expression...

Ngày tải lên: 08/10/2012, 10:25

... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... gel-forming mucins isolated from other epithelial secretions (respiratory and cervical) and from intestinal cell lines (HT-29 and PC/AA) In this example the electrophoretic mobility of the reduced ... a periodic acid-Schiff (PAS) assay and with a variety of lectins and mucin- or carbohydrate-specific antibodies (for a discussion of the relative merits and drawbacks of these analytical tools,...

Ngày tải lên: 08/10/2012, 10:25

... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... (Fullerton, CA) SW 40Ti swing-out rotor, and finally unloading and analysis of the tube contents Measure out 18 mL of M GuHCl into one chamber of the gradient maker and an equal volume of M GuHCl into ... into 0.5-mL fractions, and these were analyzed for reactivity with antisera for the MUC5AC (᭹) and MUC5B (᭺) mucins The MUC5B mucin is more polydisperse than the MUC5AC mucin and has molecules of...

Ngày tải lên: 08/10/2012, 10:25

... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine ... Research Council, Macquarie University Research Grants, National Health and Medical Research Council, and GBC Scientific Equipment (Dandenong, Victoria, Australia) Jun Yan acknowledges financial support...

Ngày tải lên: 08/10/2012, 10:25

... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal ... PerSeptive Biosystems and Beckman supply this product Quantitation of glycopeptides is quite difficult, and we recommend that 10% of the sample be first sequenced using the standard program prior...

Ngày tải lên: 08/10/2012, 10:25

... solution for h, rinse twice in MeOH for each, and air-dry overnight The pins are now ready for ELISA testing 3.3 ELISA Testing Procedure (see Notes and 12) Antibody binding to peptides on pins ... and methanol (two times) The efficiency of the cleaning procedure should be tested by repeating steps and Absorbance levels above background indicate that antibody remains bound to the pins and ... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and...

Ngày tải lên: 08/10/2012, 10:25

... lysates and media Nonspecific bands may be present and can be eliminated from consideration using the pSVL vector control lanes for comparison (Fig 2A,B, lanes and 3) The sizes of specific bands ... polymerase buffer, 2.5 mM MgCl2, 400 µM dNTPs, 200 ng of primers and or and 4, ng of template DNA (with only primers and 4), and U of Taq polymerase Perform a PCR program in a suitable thermal ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates Add µg each of primers and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for and 72˚C for...

Ngày tải lên: 08/10/2012, 10:25

... gas Program the oven temperature from 150 to 230°C and 3°C/min and then 230 to 250°C at 5°C/min Use injector and detector temperatures of 240 and 250°C, respectively Use an injection volume of ... Nakamura, M., and Ohkura, Y (1987) Fluorometric high performance liquid chromatography of N-acetyl- and N-glycolyl neuraminic acids and its application to their microdetermination in human and animal ... 164 Michalski and Capon Fig HPLC separation of sialic acid quinoxalinones obtained after mild acid hydrolysis of BSM Ac, acetyl; Lt, lactyl; Gc, glycolyl Methods 3.1 Release and Identification...

Ngày tải lên: 08/10/2012, 10:25

... (100 × cm) in a water jacket at 55°C and HPLC pump with refractive index and fluorescence detectors GlycoSepH™ and GlycoSepC™ HPLC column (Oxford GlycoSciences) Methods 3.1 Alkaline Borohydride Degradation ... 6.0, and load the sample carefully and directly to the center of the column, drop wise (see Note 3) Centrifuge for at 1100g and collect the excluded O-Glycanase O-Linked Chain Release and Fractionation ... (3 × 0.5 cm column) and elute with three column vol of HPLC-grade water Collect the effluent and eluent and then pool (2.5-mL volume) Load onto a PD10-Sephadex GM25 column and elute with HPLC-grade...

Ngày tải lên: 08/10/2012, 10:25

... was introduced in the 1970s and rapidly received a large application for analyzing the sequence of N-acetyllactosamine- and oligomannosidic-type glycans Originally, and before the development ... appearence of novel methods of ionization such as fast atom bombardment ionization (FAB), electrospray ionization (ESI), and matrixassisted laser desorption ionization (MALDI) Methods 2.1 Nuclear ... method was developed by Vliegenthart and colleagues during the 1970s and essentially applied to the structure determination of N-glycans of the Nacetyllactosamine and oligomannoside type (16) More...

Ngày tải lên: 08/10/2012, 10:25

... hygroscopic and must be thoroughly dried before weighing and making up as standards Standards of mM each of 2-, 3-, and 6-sulfo-Nacetylglucosamine in water may be aliquoted and stored at –20°C, and used ... 212 Harrison and Packer Materials 2.1 Solvents and Reagents Water, carbonate-free (see Note 1), and sparged with a constant flow of argon 0.1 M NaOH, made up with decarbonated water, and similarly ... chloride (Cl–), sulfate (HSO4–), and phosphate (H2PO3–) ions (see also Note 11) Chromatogram displays 10 nmol each of phosphate and sulfate, and trace amounts of chloride and acetate Note the difference...

Ngày tải lên: 08/10/2012, 10:25