epithelial to mesenchymal transition in prostate cancer

... Masson's trichrome staining to detect collagen and elastin The staining was carried out using Masson trichrome staining Kit (Maxim-Bio, Fuzhou, China) according to the manufacturer's instruction Western ... arrowhead), βgal staining was not detected in terminal bronchioles, although small veins were positively stained (c) The thin layer of βgal staining was observed in the sub -epithelial areas of ... results in an apparent reduction of Ecadherin staining, an epithelial marker, concomitant with its redistribution from intercellular junction areas into the cytoplasm (Fig 5a, b) In contrast, the mesenchymal...

Ngày tải lên: 12/08/2014, 16:20

... Yang J, Dai C, Wu C, Liu Y: Role for integrin-linked kinase in mediating tubular epithelial to mesenchymal transition and renal interstitial fibrogenesis J Clin Invest 2003, 112(4):503-516 Masszi ... response of the epithelium to injury In asthma the chronic cycle of injury and repair is thought to lead to the deregulation of factors involved, resulting in airway remodeling [57] Our observations ... expression of mesenchymal markers in the A549 cancer cell line, without evidence of induction of EMT by IL-1β alone The inflammatory cytokine IL-1β is elevated in BAL fluid of symptomatic asthmatics...

Ngày tải lên: 12/08/2014, 14:20

... keratin 14 overexpression in IDC 110 Figure 29 Maspin immunostaining in normal breast epithelia 118 Figure 30 Maspin immunostaining in DCIS of the breast 119 Figure 31 Maspin immunostaining in ... allowed to dry at room temperature for minute before staining 3.2.2.2 Hematoxylin Staining The sections were stained in RNase-free Gill’s III hematoxylin for 30 seconds and dehydrated with increasing ... while mesenchymal markers are upregulated Downregulated E-cadherin Claudin Occludin Desmoplakin Cytokeratin Upregulated N-cadherin OB-cadherin/cadherin-11 Vimentin Fibronectin Osteonectin/SPARC...

Ngày tải lên: 05/10/2015, 21:24

... attached to a cannula with saline-diluted Gd-DTPA (Magnevist ® , Schering, Berlin, Germany) was inserted into the tail vein Dynamic T1-weighted (T1W) imaging was acquired by performing 20 minutes ... when using a BPNN, care should also be taken when training the network, in order not to under- or overtrain it [25] Since our RMSE function (Figure 4A) from training, testing and validating the ... in addition to treatment groups (binary encoded), were used as input variables For this simulation, the RMSE increased after 30 epochs, indicating overtraining Thus, the optimal training for the...

Ngày tải lên: 09/08/2014, 09:20

... arrowhead-indicated in (a)) In the pCX-shh-IG-injected prostates, as the prostates were induced into PIN and progressed into CaP status, these P63 + /CK14(low or -) cells appeared to be differentiated ... pCX-shh-IG injection (arrowheadindicated in (c) and (d), also magnified in the inlets) (C) The pCX-shh-IG-injected prostate tissue sections were stained strongly for Hedgehog protein in PIN and CaP, in ... with tumor initiation and progression in the pCX-shh-IG-injected prostate (arrow-indicated in (b), (c) and (d)), in contrast to the normal P63+ cells in the pCX-IG-injected prostate (indicated...

Ngày tải lên: 10/08/2014, 05:21

... This suggests that in addition to regenerating damaged alveolar epithelial lining [12], AECII may undergo epithelial- mesenchymal transition (EMT) to contribute to foci development in the disease ... and CGZ partially inhibited the maximum increase in N-cadherin expression in response to 2.5 ng/ml TGF-b1 (Figure 4B) The inhibitory effects of RGZ and CGZ were maintained in the presence of ... collected following centrifugation at 13,000 g (5 min, 4°C) and stored at -20°C Total protein concentration was measured via a spectrophotometer (Thermoplus, Finland) using the bicinchoninic acid (BCA)...

Ngày tải lên: 12/08/2014, 11:20

... contributes to nearly 25% of all prostate cancer cases The risk of getting prostate cancer increases nearly exponentially with increasing age This makes prostate cancer one of the most distinctive cancers ... Prostate Cancer 617 Chapter 25 Integrins in Prostate Cancer Invasion and Metastasis 619 Paulynn Chin Suyin, Joanne Louise Dickinson and Adele Frances Holloway Chapter 26 The Role of E-Cadherin-Catenin ... Prada Section Prostate Cancer Markers Chapter Testosterone Measurement and Prostate Cancer 157 Tine Hajdinjak Chapter Describing Prostate Cancer Dynamics: Second Look at PSADoubling Time and PSA-Specific...

Ngày tải lên: 22/03/2014, 22:20

... Cambridge, MA) Staining was scored on a four level scale (0 = no staining, = weak staining, = moderate staining, = intense staining) by a GU pathologist Results Expression Profiling of Ovarian Cancer Patients ... relocates to the spindle equator [4] Aurora-B kinase is known to regulate processes such as kinetochore and microtubule interactions [5-8] and cytokinesis [9,10] Aurora-C is expressed specifically in ... aneuploidy, possibly due to the inhibition of other family members such as Aurora-B Thus, in addition to inhibiting mitotic entry, VE-465 appears to induce apoptosis by causing catastrophic chromosomal...

Ngày tải lên: 18/06/2014, 15:20

... collection Urine, up to 200 ml volume, collected into sterile containers (Millipore), was brought to the laboratory for processing within 30 minutes Samples were collected mid to late morning, and ... produced by LNCaP -prostate cancer cell line Prostate cancer cell lines (LNCaP and DU145), as indicated, were maintained in culture as a source of positive-control prostate cancer exosomes (for ... of exosomes present Prostate Cancer cell lines produce typical exosomes, positive for prostate and cancer- associated antigens Two prostate cancer cell lines were maintained in culture, as a source...

Ngày tải lên: 18/06/2014, 15:20

... could exert cytotoxicity against AMACR-expressing prostate cancer cell lines in an HLA-A*2402-restricted manner, we examined their cytotoxic activity against prostate cancer cell lines that express ... Sato N: Aberrant expression and potency as a cancer immunotherapy target of inhibitor of apoptosis protein family, Livin/ML-IAP in lung cancer Clin Cancer Res 2005, 11:1000-1009 Sato T, Sato ... against HLA-A24-positive AMACR-expressing prostate cancer cell lines Figure Cytotoxic activities of AMACR peptide-specific CTLs against HLA-A24-positive AMACR-expressing prostate cancer cell lines...

Ngày tải lên: 18/06/2014, 15:20

... macrophage infiltration with angiogenesis and prognosis in invasive breast carcinoma Cancer Res 1996, 56(20):4625-4629 19 Pollard JW: Macrophages define the invasive microenvironment in breast cancer ... in colon cancer Clin Cancer Res 2007, 13(5):1472-1479 37 Nagorsen D, Voigt S, Berg E, Stein H, Thiel E, Loddenkemper C: Tumorinfiltrating macrophages and dendritic cells in human colorectal cancer: ... metastasis in colorectal adenocarcinoma based on the combination of risk factors including CD10 expression: multivariate analysis of clinicopathological and immunohistochemical factors Oncol...

Ngày tải lên: 18/06/2014, 16:20

... differentiated into macrophages, makes these cells prone to HIV infection Since HMM forms of A3G are reported to be enzymatically inactive, if A3G remains in HMM complex in prostate epithelial cells, ... prostate epithelial cell lines specifically in LNCaP cells [3,18] In addition, the prostate cancer cell line 22Rv1 has been shown to be chronically infected with XMRV and produces highly infectious ... replicates in prostate epithelial cancer cell lines since these cells lack or express undetectable levels of A3G [13-16] In this report, we demonstrate that prostate epithelial cell lines LNCaP...

Ngày tải lên: 18/06/2014, 18:20

... were seen with the JNK inhibitor, JAK3 inhibitor, piceatannol, Wortmannin, MET inhibitor and SIS3 However, it is interesting to note that the Akt inhibitor reversed the inhibitory effects of rhMDA-7 ... Nottingham, UK) and ABCAM (Cambridge, UK) ROCK inhibitor was from Santa-Cruz Biotechnologies, Inc (Santa Cruz, CA, USA), Akt inhibitor, SIS3 inhibitor, PLC-gamma inhibitor, JNK inhibitor, JAK inhibitor, ... of our continuing studies to investigate proteins interacting with TGase-4 using immunoprecipitation of proteins from the prostate gland, we identified a small panel of proteins that interacted...

Ngày tải lên: 18/06/2014, 19:20

... were seen with the JNK inhibitor, JAK3 inhibitor, piceatannol, Wortmannin, MET inhibitor and SIS3 However, it is interesting to note that the Akt inhibitor reversed the inhibitory effects of rhMDA-7 ... Nottingham, UK) and ABCAM (Cambridge, UK) ROCK inhibitor was from Santa-Cruz Biotechnologies, Inc (Santa Cruz, CA, USA), Akt inhibitor, SIS3 inhibitor, PLC-gamma inhibitor, JNK inhibitor, JAK inhibitor, ... of our continuing studies to investigate proteins interacting with TGase-4 using immunoprecipitation of proteins from the prostate gland, we identified a small panel of proteins that interacted...

Ngày tải lên: 20/06/2014, 03:20

... value concerning the primary detection and the staging of prostate cancer [27] In principle, signal-generation is based on an increased choline metabolism in prostate cancer leading to an increased ... [39] In such a geometrical setting it is defined as: TCP  e  n i SFi i SF i is the surviving fraction within the single subvolume with the running index i (ranging from to m = V/vi) Using the ... planning in prostate cancer Q J Nucl Med Mol Imag 2009, 53(2):245-268 28 Reske SN, Blumstein NM, Glatting G: [C-11]choline PET/CT imaging in occult local relapse of prostate cancer after radical prostatectomy...

Ngày tải lên: 09/08/2014, 08:22

... perifosine and the prototypic intravenously applicable ErPC3, in preclinical and clinical investigations is based on their ability to induce apoptosis in tumor cells and their ability to increase ... inhibitors of the PI3K/Akt pathway in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate cancer cell lines Up to now our data revealed that ErPC3 is a potent inhibitor ... serine 473 but phosphorylation on serine 473 seems to be independent of PDK1 Although several kinases, such as integrinlinked kinase, DNA-dependent protein kinase (DNAPK), and the mTOR/Rictor-complex...

Ngày tải lên: 09/08/2014, 09:20

... becoming one of the most significant issues in treatment decision-making, in general, and more so in prostate cancer [1] Thus late rectal and urinary damage became a major concern in prostate cancer; ... according to RTOG recommendations (treatment of only subaortic presacral LNs, contours of common iliac vessels starting at the L5/S1 interspace, external iliac contours stopping at the top of ... GI and GU toxicity observed in patients during their last inspection are shown In the cohort of patients included in the WP group, pretreatment GI toxicity of grade was found in the history of...

Ngày tải lên: 09/08/2014, 09:20

... Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival signal transduction pathways Int ... were in S-phase, 65% in G0/G1 and 20% in G2/M In the following, the S-phase was reduced to 5%, while G0/G1 increased to 73% The proportion of cells in G2/M was only minimal changes After incubation ... genistein pretreatment could be shown Furthermore, a significant increase in cleaved PARP protein was measured following combined genistein and radiation treatment, indicating increased apoptosis...

Ngày tải lên: 09/08/2014, 09:22