epigenome and dna methylation in oral squamous cell carcinoma

... negatively in uence tumor progression Soluble E-cadherin has been shown to disrupt cell cell adhesion in cultured epithelial cells [13] Transfection of E-cadherin cDNA into invasive carcinoma cells ... 27 Truncated P-cadherin in oral squamous cell carcinoma 120 kDa 120 kDa 50 kDa 50 kDa Beta akt C M Fig Truncated P-cadherin in cell lysates and supernatants of OSCC cell lines (A) Western blot ... CK19) and differentiating and terminally differentiated cells, respectively (CK10, involucrin) OSCC cell lines PCI 13, PCI 68 and PCI showed expression of all markers The cell lines PCI and PCI...

Ngày tải lên: 30/03/2014, 04:20

... staining for Oct4 and Sox2 expression in hypopharyngeal squamous cell carcinoma Brown grains represent a positive signal (3, 3-diaminobenzidine staining) The positive expression site of Oct4 and ... Huang CY, Lin SC, Liu CJ, Tsai TH, Chou SH, Chien CS, Ku HH, Lo JF: Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma Clin Cancer ... patients with hypopharyngeal squamous cell carcinoma, suggesting that Oct4 expression may be a useful indicator for predicting the prognosis of hypopharyngeal squamous cell carcinoma Acknowledgements...

Ngày tải lên: 18/06/2014, 16:20

... Characteristics of mutations in the p53 gene in oral squamous cell carcinoma associated with betel quid chewing and cigarette smoking in Taiwanese Carcinogenesis 2001, 22:1497-1503 Adelstein DJ, Li Y, Adams ... complex between DNA- dependent protein kinase and EGFR and then delayed the DNA repair [37-39] Oxaliplatin induced double-strand breaks [40] When cetuximab was combined with oxaliplatin, cetuximab ... blockade in squamous cell carcinomas: inhibition of damage repair, cell cycle kinetics, and tumor angiogenesis Clin Cancer Res 2000, 6:2166-2174 36 Nyati MK, Morgan MA, Feng FY, Lawrence TS: Integration...

Ngày tải lên: 18/06/2014, 19:20

... Characteristics of mutations in the p53 gene in oral squamous cell carcinoma associated with betel quid chewing and cigarette smoking in Taiwanese Carcinogenesis 2001, 22:1497-1503 Adelstein DJ, Li Y, Adams ... complex between DNA- dependent protein kinase and EGFR and then delayed the DNA repair [37-39] Oxaliplatin induced double-strand breaks [40] When cetuximab was combined with oxaliplatin, cetuximab ... blockade in squamous cell carcinomas: inhibition of damage repair, cell cycle kinetics, and tumor angiogenesis Clin Cancer Res 2000, 6:2166-2174 36 Nyati MK, Morgan MA, Feng FY, Lawrence TS: Integration...

Ngày tải lên: 20/06/2014, 03:20

... PCI1-1 and PCI 52 In this cell line, there was a significant staining by mAb 57B (Figure 11) Nearly all of the cells were stained in the Figure cell magnification) staining line PCI 1-1 hematoxylin ... counterstaining Tumour cell line PCI 68-1 with immunocytochemical MAGE-A staining and simultaneous hematoxylin counterstaining (400× magnification) No staining with MAGE-A Ab of this tumour cell line ... other tumour cell lines expressing only MAGE-A2, -A3 and -A6 In these examinations, further determination of the role of MAGE-A4 in the progression and prognosis of oral squamous cell carcinoma might...

Ngày tải lên: 11/08/2014, 20:20

... HR HPV DNA in Table HPV detection by HC-II assay and PCR Method in Clinico-pathogical parameter (OSMF grading according to Pindborg and T and N category according to UICC classification) in OSMF ... (PCR): Polymerase chain reaction; (OSMF): Oral submucous fibrosis; (HNSCC): head and neck squamous cell carcinoma; (HCII): Hybrid Capture; (OSCC): Oral squamous cell carcinoma Acknowledgements ... detection by HC-II assay and PCR Method in Clinico-pathogical parameter (OSMF grading according to Pindborg and T and N category according to UICC classification) in OSCC cases Clinico-pathogical Diagnosis...

Ngày tải lên: 12/08/2014, 01:22

... adenocarcinoma cell lines (Caco-2 and DLD1), a human cervix epithelioid carcinoma cell line (HeLa), a human lung adenocarcinoma cell line (A549), and human hematological malignant cell lines ... including development and cancer [16] EMT in cancer leads to the loss of cell- cell adhesion and cell polarity as well as altered cell- extracellular matrix interactions, resulting in invasion and ... modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox expression in esophageal squamous cell carcinoma cells Journal of Translational Medicine 2011 9:30 Submit...

Ngày tải lên: 18/06/2014, 19:20

... intensity of staining; no staining, weak staining, moderate staining and strong staining Since tumor cells stained uniformly across the samples we did not considered the fraction of tumor cells with ... EphA2 (A-D) and EphrinA-1 (E-H) showing cases with no staining (A, E), weak staining (B, F), moderate staining (C, G) and strong staining (D, H) In previous reports [28, 29] protein expression ... the cell cycle proteins p21, p27, p16, cyclin A, cyclin E and cyclin D3 in early squamous cell cervical carcinomas These studies may indicate that the mechanisms of expression EphA2 and EphrinA-1...

Ngày tải lên: 08/08/2014, 17:20

... staining Cells were dissociated and fixed in PBS containing 1% glutaraldehyde for h After washing in PBS, cells were stained with 200 μM Hoechst 33342, mounted on slides, and visualized using ... after BNCT, and this was maintained until 48 h An increase in the phosphorylation of cdc2 occurred at 12 h, indicating cell cycle arrest at the G2 checkpoint, and declined to the initial level ... provided cell lines and participated in the design of the study TO provided cell lines and participated in the design of the study YY conceived of the study and participated in its design and coordination...

Ngày tải lên: 09/08/2014, 10:20

... focus on the influences of age, gender, nicotine consumption, and tumour stage Methods In a cross-sectional study, 73 patients with oral squamous cell carcinomas of the tongue, gingiva, buccal ... manuscript, RS and SSY participated in the design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Competing interests The authors ... difficulties in understanding the questions, but did not influence the answers The EORTC-QLQ-C30 questionnaire is concerned with malignant diseases and their treatment in general, and contains 30 questions...

Ngày tải lên: 11/08/2014, 20:20

... Cytologic detection of esophageal squamous cell carcinoma and its precursor lesions using balloon samplers and liquid based cytology in asymptomatic adults in Linxian, China Acta Cytol 2008, 52:14-23 ... participated in the design of the study, helped analyze the data, and was involved in writing and editing the manuscript All authors read and approved the final manuscript Page of 16 17 18 19 20 Competing ... Kun-He Z, Bi-Ming L, Jiang C: Value of endoscopic methylene blue and Lugol’s iodine double staining and detection of GST -and telomerase in the early diagnosis of esophageal carcinoma World J...

Ngày tải lên: 18/06/2014, 16:20

... Dharamsi S, Thinh DH, Hong NT, Cuc TT, Thao NT: The oral squamous cell carcinoma and cultural oral risk habits in Vietnam Int J Dent Hyg 2010, 8:159-168 Marocchio LS, Lima J, Sperandio FF, Correa ... SO: Oral squamous cell carcinoma: an analysis of 1,564 cases showing advances in early detection J Oral Sci 2010, 52:267-273 Gao W, Guo CB: Factors related to delay in diagnosis of oral squamous ... squamous cell carcinoma J Oral Maxillofac Surg 2009, 67:1015-1020 Hendrikx AWF, Maal T, Dieleman F, Van Cann EM, Merkx MAW: Cone-beam CT in the assessment of mandibular invasion by oral squamous cell...

Ngày tải lên: 10/08/2014, 23:21

... Cancer stem cells in Head and Neck Squamous Cell Carcinoma In head and neck squamous cell carcinoma (HNSCC), Prince et al first isolated a highly tumourigenic subpopulation of cancer cells that ... population of cells with cancer stem cell characteristics in head and neck squamous cell carcinoma (HNSCC) The aim of this study is to investigate the expression of CD44s in squamous cell carcinoma (SCC) ... cancer on the decline in the developed world, a rising incidence suggests a continuing increase in the absolute numbers of cases to be treated in the coming decades.3 In India, incidence rates...

Ngày tải lên: 02/10/2015, 17:14

... expression using PAR-2 AS ODN in AGS cells Additionally, inhibition of PAR-2 activity using trypsin inhibitor increased cell death and DNA fragmentation in H pylori-infected AGS cells These results demonstrate ... and integrins in gastric epithelial cells [21,22] Trypsin activated PAR-2, which mediated the proliferation of various cells including pancreatic and gastric cancer cells, and smooth muscle cells ... oligonucleosome-bound DNA in the cell lysates using a sandwich ELISA (Cell Death Detection ELISAplus kit; Boehringer-Mannheim) The relative increase in oligonucleosome-bound DNA was determined at 405 nm and expressed...

Ngày tải lên: 18/06/2014, 16:20

... +2, +3 for increasing intensity and “continuity” of staining of the cell membrane[17] For quantification of Her3 staining, we scored two compartments of the cell - the cell membrane, using the same ... (DakoCytomation, Carpinteria, CA) at 95°C, for 10 and 40 min, for Her3 and Her2 staining respectively To minimize non-specific staining, the section was incubated with protein block (DakoCytomation) ... peroxidase kit (Dako) The stain visualized using 3,3’-diaminobenzidine plus (Dako) and was lightly counterstained with hematoxylin, dehydrated in ethanol, and cleared in xylene Appropriate negative...

Ngày tải lên: 18/06/2014, 22:20

... strong (3+), and 26% showed no TLR4 staining (Figure 1; Table 3) TLR4 staining (all scores) showed a diffuse and fine granular cytoplasmatic pattern Distinct membrane staining was observed in some ... (AB) The percentage of tumor cells showing a positive membranous/cytoplasmatic staining and the intensity of staining were assessed Cases with complete lack of staining were scored as negative, ... causing a threonine/isoleucine switch at amino acid location Thr399Ile (rs4986791) These polymorphisms alter the amino acid sequence of the TLR4 protein and affect the extracellular domain and...

Ngày tải lên: 18/06/2014, 22:20

... +2, +3 for increasing intensity and “continuity” of staining of the cell membrane[17] For quantification of Her3 staining, we scored two compartments of the cell - the cell membrane, using the same ... (DakoCytomation, Carpinteria, CA) at 95°C, for 10 and 40 min, for Her3 and Her2 staining respectively To minimize non-specific staining, the section was incubated with protein block (DakoCytomation) ... peroxidase kit (Dako) The stain visualized using 3,3’-diaminobenzidine plus (Dako) and was lightly counterstained with hematoxylin, dehydrated in ethanol, and cleared in xylene Appropriate negative...

Ngày tải lên: 20/06/2014, 04:20

... strong (3+), and 26% showed no TLR4 staining (Figure 1; Table 3) TLR4 staining (all scores) showed a diffuse and fine granular cytoplasmatic pattern Distinct membrane staining was observed in some ... (AB) The percentage of tumor cells showing a positive membranous/cytoplasmatic staining and the intensity of staining were assessed Cases with complete lack of staining were scored as negative, ... causing a threonine/isoleucine switch at amino acid location Thr399Ile (rs4986791) These polymorphisms alter the amino acid sequence of the TLR4 protein and affect the extracellular domain and...

Ngày tải lên: 20/06/2014, 04:20

... conventional determination of L1 in HEstain (hematoxylin-eosin) with nodal metastasizing Currently, for some malignant tumors (e.g breast carcinoma, carcinoma of uterine cervix, or esophageal carcinoma) ... someconventional ×400) by inflammatory vesselin in lymphatic vessel endothelium in Lymphangiosis carcinomatosa in lymphatic (HEsurrounded a) Lymphangiosis carcinomatosa in conventional staining (HE ×400); ... HUV was involved in the idea, histopathology, evaluation of staining, manuscript IN was involved in the evaluation of staining, results MS, RH, CV were involved in clinical data and details, discussion...

Ngày tải lên: 09/08/2014, 04:21