epigenetics of solid cancer stem cells

... P63+ basal /stem cells were transformed into AR+ or ARcancer cells but rarely into ChgA+ neuroendocrine cancer cells Conventional human prostate cancer cells include malignant cells of luminal, ... basal/ stem cells Patch1 and Smo were found highly expressed in the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells in regions of ... biology of the prostate: stem cell biology Urology 2003, 62:11-20 Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development of prostate...

Ngày tải lên: 10/08/2014, 05:21

... According to the hierarchical model of cancer, the origin of the cancer stem cells may be long-lived somatic stem cells Therefore, markers of “normal” stem cells are being sought with the expectation ... neoplastic mechanism of liver cancer stem cells Conclusions This work provides an in vivo model for the study of mechanisms of neoplastic transformation of liver cancer stem cells by separately ... carcinogenesis and differentiation of normal stem cells strongly suggest that miRNA may be involved in the transformation of normal stem cells into cancer stem cells Therefore, screening for differences...

Ngày tải lên: 10/08/2014, 10:20

... Page 13 of 13 18 Seo DC, Sung JM, Cho HJ, Yi H, Seo KH, Choi IS, Kim DK, Kim JS, ElAty AMA, Shin HC: Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells Mol Cancer ... markers of cancer stem cells Their expression in tumor cells could correlate with their aggressive biological behavior, drug resistance and poor prognosis, which are common characteristics of LCNEC ... et al.: Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung An FFPE proteomic study Journal of Clinical Bioinformatics 2011 1:23...

Ngày tải lên: 10/08/2014, 09:22

... al Journal of Experimental & Clinical Cancer Research 2010, 29:174 http://www.jeccr.com/content/29/1/174 cancer stem cells http://www.mirbase.org/ Thus, the identification of cancer stem cell-related ... regulatory factor in cancer cells, even cancer stem cells Another example is miR21, which has been reported to have extensive roles and is expressed in embryonic stem cells [29], neuronal cells [30] and ... microarrays for BCSC cells Table S1-MiRNAs microarray- based miRNAs expression profile of MCF-7 cells (signal value ≥800) The table shows the miRNAs expression profile of MCF-7 cells obtained through...

Ngày tải lên: 10/08/2014, 10:20

... by comparison of the relative strength of selection with non -stem cells could furthermore provide a method of testing the relative strength of selection of stem cells and non -stem cells and thereby ... size of tumour, because of the much smaller number of stem cells, clonal evolution is predicted to be faster in stem cells compared to non -stem cells What are the mechanisms by which one cancer ... representing a tumour of a given size The number of stem cells is calculated by assuming one stem cell per 57 000 non -stem cells [28] A greater proportion of stem cells would result in a less dramatic difference...

Ngày tải lên: 13/08/2014, 16:20

... primitive cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells ... comprising less than 0.1% of the tumor mass, has many of the properties of cancer stem cells [4] In particular, self renewal, extended lifespan (compared to normal stem cells) , a high invasive ... cultures of cancer stem cells is likely to be important for maintaining gene expression patterns similar to cancer stem cells in vivo Genome Biology 2008, 9:R83 Genome Biology 2008, Cancer BPH...

Ngày tải lên: 14/08/2014, 08:21

... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... and m-FISH (Figure 3) of metaphase spreads Hence, the results of this study could have useful implications for the salvage of hESC culture during infrequent occurrences of incubator break-down...

Ngày tải lên: 31/10/2012, 16:57

... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact that RNA ... both hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed...

Ngày tải lên: 07/03/2014, 12:20

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for ... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

Ngày tải lên: 22/03/2014, 09:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... expression profiling We assessed global hES gene expression profiles on separate passages of cells from different hES cell lines, EB samples at different time points, and types of adult cells, HUVEC, ... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of...

Ngày tải lên: 18/06/2014, 15:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

Ngày tải lên: 18/06/2014, 19:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... mean fat cells size and fat cells area would be measured with imaging process software Image-Pro Plus 5.1 (Media Cybernetics Inc., Silver Spring, MD) Fat cells density = marrow fat cells number...

Ngày tải lên: 20/06/2014, 00:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

Ngày tải lên: 20/06/2014, 04:20

... Origin of cancer stem cells Self-renewal and differentiation potentials are the features of stem cells Origin of cancer stem cells Self-renewal and differentiation potentials are the features of stem ... stem cells, progenitor cells, mature cells, and cancer cells The genetic program controlling self-renewal and differentiation plays a key role in the genesis of cancer stem cells (Figure 1) Cancer ... hypothesis is that cancer stem cells are caused by transforming mutations occurring in multi-potential stem cells, tissue-specific stem cells, progenitor cells, mature cells, and cancer cells described...

Ngày tải lên: 20/06/2014, 07:20

... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult-derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... brain originate from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the ... progenitor or stem cells locally would represent a strategy to promote regeneration of the diseased and injured nervous system Alternatively, new neuronal cells are generated at sites of degeneration...

Ngày tải lên: 08/08/2014, 17:20

... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle-derived stem cells, or satellite cells A recent ... involvement of Wnts is complicated by their pleiotropic effects Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo reconstitution of ... is the homing of stem cells to sites of injury and subsequent wound healing Although some tissue repair may be accomplished by the division of indigenous differentiated cells, such cells are most...

Ngày tải lên: 09/08/2014, 10:20

... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... suppression of chordin led to an increase in the bioavailability of endogenously produced BMP-2 to drive the differentiation of Page of (page number not for citation purposes) Effect of chordin ... extrapolated to cells from a large population of donors because there is consistently a significant and large increase in expression of osteogenic markers, as a result of chordin knockdown in cells from...

Ngày tải lên: 09/08/2014, 10:23

... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8 ... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... cartilage, and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in the...

Ngày tải lên: 09/08/2014, 14:22