epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells pdf
Regulation of gene expression by esrrb in embryonic stem cells
Ngày tải lên :
12/09/2015, 08:19
... Examples of pluripotent stem cells include the embryonic stem (ES) cells, embryonic germ (EG) cells and embryonal carcinoma (EC) cells Multipotent stem cells can give rise to cells of certain ... OF FIGURES LIST OF PUBLICATIONS LIST OF ABBREVIATIONS CHAPTER I INTRODUCTION 1.1 Sources and properties of pluripotent stem cells 1.1.1 Mouse embryonal carcinoma cells 1.1.2 Mouse embryonic stem ... properties of pluripotent stem cells Pluripotent stem cells can be isolated from various embryonic sources For instance, EC cells can be derived from teratocarcinomas, while ES cells and EG cells...
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Establishment of autologous culture systems for human embryonic stem cells
Ngày tải lên :
11/09/2015, 10:00
... Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff ... types of cells which are in the closely related families (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
Ngày tải lên :
11/09/2015, 10:14
... vitro system to study complex events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic ... PUBLICATIONS 161! vii SUMMARY Human embryonic stem cells (hESC) are pluripotent stem cells that have the unique ability to differentiate into cells of the three germ line lineages Hence, they ... variety of cells that make up the central nervous system including dopaminergic neurons, motor neurons, glial cells, astrocytes, oligodendrocytes, neural crest stem cell cells and retinal cells...
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Báo cáo y học: " Regulation of Sox9 activity by crosstalk with nuclear factor-κB and retinoic acid receptors" pptx
Ngày tải lên :
09/08/2014, 10:22
... compared with cells transfected with reporter alone (compare Figure 7b and Figure 4b; P < 0.001) To determine the effect of NF-κB activation by caMEKK1 on RAR function, cells were co-transfected with ... were analyzed by densitometry using Kodak Digital Science software (Eastman Kodak, Rochester, NY, USA) Data were analyzed by paired t-tests, or by analysis of variance followed by Tukey's multiple ... of NF-κB was not associated with changes in nuclear levels of NF-κB p65 or RARα We next evaluated the possibility that RAR activation changes the binding of NF-κB to DNA Nuclear extracts of cells...
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THE DIRECT REPROGRAMMING OF SOMATIC CELLS: ESTABLISHMENT OF A NOVEL SYSTEM FOR PHOTORECEPTOR DERIVATION
Ngày tải lên :
24/08/2014, 12:37
... as embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) or neural stem cells [14–17, 19–21] These protocols are often based upon culturing the stem cells in culture medium with ... neuralization of somatic cells induced by combinations of known pro-neural genes, and finally, the induction of photoreceptor-like phenotypes in somatic cells 2.1 Selection of candidate genes While many of ... The Direct Reprogramming of Somatic Cells: Establishment of a Novel System for Photoreceptor Derivation Major Professor: Jason S Meyer Photoreceptors are a class of sensory neuronal cells that...
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Báo cáo khóa học: Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody pdf
Ngày tải lên :
16/03/2014, 16:20
... spectra were externally calibrated with the molecular mass of a mixture of standard peptides Results Comparison of ligand/Gb3 binding by RELISA The binding of VT1, VT2, and mAb anti-Gb3 (38.13) ... detection system (Fig 6) Conjugation of VT1B with FITC significantly Basis for lack of VT1B renal type B glomerular staining The basis of the differential VT1 and anti-Gb3 binding of the type ... above, and stained with mouse anti-SGC SULF1 (2 lgÆmL)1) followed by incubation with HRP-conjugated goat anti-mouse Ig (1 : 500) Each step consisted of a 30 incubation followed by TBS washing SULF1...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
Ngày tải lên :
23/03/2014, 07:20
... FEBS 1625 Epigenetic study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and ... DNA methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from ... apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells DNA methylation...
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Báo cáo khoa học: PKCd-dependent cleavage and nuclear translocation of annexin A1 by phorbol 12-myristate 13-acetate pdf
Ngày tải lên :
23/03/2014, 21:20
... ANX-1 As ANX-1 is cleaved by treatment with PMA in epithelial A549 cells [7], we first examined whether PMA induces the cleavage of ANX-1 in HEK 293 cells Cells were treated with 10 nM PMA for 30 ... form of ANX-1 was detected in PMAstimulated HEK 293 cells, whereas only the intact form of ANX-1 was found in control cells ANX-1 was partially cleaved by the treatment of PMA and most of ANX-1 ... Immunostaining was performed by incubation with anti-ANX-1 monoclonal antibody (0.05 lgÆmL)1) for h After washing the cells with NaCl/Pi three times, the cells were incubated with TRITC-conjugated goat...
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Báo cáo Y học: Thermodynamics and kinetics of the cleavage of DNA catalyzed by bleomycin A5 A microcalorimetric study pdf
Ngày tải lên :
24/03/2014, 04:21
... cleavage of calf thymus DNA by a mixture of BLM-A5, Fe2+ and O2 with those of five control systems (a) A reaction system containing 21.5 lM BLM-A5, 20 lM Fe2+ and 15.2 lM calf thymus DNA saturated with ... UV-vis spectra of BLM-A5 Figure 3A compares the UV and visible spectrum from 250 to 500 nm of BLM-A5 after the cleavage of calf thymus DNA by a mixture of BLM-A5, Fe2+ and O2 with those of the five ... the catalytic efficiency (represented by k2/Km) of BLM-A5 is of the same order of magnitude as that of lysozyme but several orders of magnitude lower than those of TaqI restriction endonuclease,...
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báo cáo hóa học:" Association between neuroserpin and molecular markers of brain damage in patients with acute ischemic stroke" pdf
Ngày tải lên :
20/06/2014, 03:20
... This research was carried out in accordance with the Declaration of Helsinki of the World Medical Association (2000) and approved by the Ethics Committee of the participating hospital Informed consent ... probability of entry P < 0.05) Due to a lack of linearity, the decrease of neuroserpin levels at 24 hours was categorized by ROC analysis Results were expressed as adjusted odds ratios (OR) with the ... volume treated with tPA [6], which is in line with the results of the present manuscript, where a greater decrease in neuroserpin serum levels within the first 24 hours was correlated with lower...
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Báo cáo toán học: " Synthesis and characterization of CuO nanowires by a simple wet chemical method" pdf
Ngày tải lên :
20/06/2014, 21:20
... obtained Thus, the presence of a small amount of TG can render the nanowires of CuO welldispersed, as seen clearly in the micrograph of CuO with TG The average diameter of the CuO nanowires was ... prepared (a) without TG and (b) with TG Figure XRD pattern of the as-synthesized CuO nanowires with TG Figure The FTIR spectrum of the as-synthesized CuO nanowires in the presence of TG Figure ... The morphology of the CuO samples without TG shows the formation of CuO flowers consisting of individual nanowires, whereas when the same synthesis is carried out in the presence of organic molecules...
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Báo cáo hóa học: " Research Article Capacity of Wireless Ad Hoc Networks with Opportunistic Collaborative Communications" pdf
Ngày tải lên :
22/06/2014, 22:20
... allowed by the MH protocol with no spatial reuse Here, for convenience of analysis, we restate the problem of maximizing Rsd in the following equivalent way Find (i) the sequence of M + (with ≤ ... capacity region achievable by OST is larger than that obtained by MH This conclusion is obtained by exploiting the results in [6] and by casting the optimal MH problem of [2] in a suitable framework ... Section 3, the capacity region of OST is larger than that of MH due to the power gain that node can capitalize upon by collaborating with both nodes and while communicating with through OST Considering...
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Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx
Ngày tải lên :
07/08/2014, 23:22
... bloc ceramics with pores precultured with cells in osteogenic medium or vacuumed for uniform loading and retention of cells [13,34] In the present study, β-TCP granules were mixed with cells immediately ... implantation Canine UCB-MSCs were suspended with 500 μl of normal saline prior to mixing with 700 mg of β-TCP (group CM) The same volume of normal saline mixed with β-TCP was prepared as the control ... only one of six interfaces in group C by 12 weeks (Fig 2: proximal interface of C1) In group CM, continuity was established at three of six interfaces by 12 weeks (Fig 2: proximal interface of CM1,...
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Báo cáo y học: "Anti-Sa antibodies and antibodies against cyclic citrullinated peptide are not equivalent as predictors of severe outcomes in patients with recent-onset polyarthritis" pdf
Ngày tải lên :
09/08/2014, 06:22
... role of anti-Sa and of anti-CCP antibodies, relative to RF, in patients with polyarthritis evaluated and treated early after the onset of disease, irrespective of the fulfillment or not of diagnostic ... erosion component of the SvH score of at least The predictive ability of anti-Sa, anti-CCP, and RF in the early identification of patients with each outcome was also studied by using the area ... minimized by the strict exclusion of patients with diseases known to have a good prognosis (such as crystal-induced arthritis and monoarthritis) and by a retention rate of 90% at 30 months, without...
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Báo cáo y học: "The NFI-Regulome Database: A tool for annotation and analysis of control regions of genes regulated by Nuclear Factor I transcription factors" pdf
Ngày tải lên :
10/08/2014, 09:22
... binding sites, aligned by ATG as The height of the bar indicates the number of binding sites of each type within the bin of 1000 bp Bins can be adjusted in size Proposed uses of the database In its ... [23] This functionality is currently provided by the software layer of the NFI-Regulome database and by taking advantage of the features provided by the underlying database we define these constraints ... are presented at the top of the page with the number of TFs and TF binding sites (TFBS) and a list of the sites and their locations In detail, every site is listed along with its location, sequence,...
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Báo cáo y học: "Are acupoints specific for diseases? A systematic review of the randomized controlled trials with sham acupuncture controls" pdf
Ngày tải lên :
13/08/2014, 15:21
... effect of the drug “Huperzine A” on “NMDA Receptor” (drug target) A collection of linked RDF statements forms a directed acyclic graph (DAG) Such collections of statements represent the knowledge of ... resource as a class, e.g Herb A hierarchy of classes can be defined using the rdfs:subClassOf construct For example, “Huperzia serrata” is a subclass of “Herb” Most of the RDFS components are included ... to properties (e.g the “inhibit” property can have a minimum cardinality of one Page of and cardinality with a maximum of a positive integer) While OWL is semantically richer than RDF or RDFS,...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor FISH ... expression of SSEA-3 was indicated by green fluorescence under a wavelength of 490 nm (FITC), while positive expression of TRA-1-81 was indicated by red fluorescence under a wavelength of 570 nm ... Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: somatic...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
Ngày tải lên :
07/03/2014, 03:20
... proportion of positive cells was classified into five groups: 0, no tumor cells exhibiting IR; 1, < 25% of the tumor cells exhibiting IR; 2, 25–50% of the tumor cells exhibiting IR; 3, 50–75% of the ... activation of P-gp in gastric cancer cells transfected with PrPC Inhibition of the PI3K/Akt pathway by its specific inhibitor or Akt siRNA reverses PrPC-induced MDR in gastric cancer cells, partly by ... calculated relative to the amount of b-actin protein promoter in gastric cancer cells decreased with or without PrPC transfection by adding LY294002 or co-transfection with Akt siRNA However, SGC7901...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
Ngày tải lên :
07/03/2014, 12:20
... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... interaction of RNA Pol II with the b-globin gene locus in mouse embryonic fibroblasts (MEFs) and OP9 stromal cells (OP9) These cells were used in our subsequent studies to support the growth of undifferentiated ... differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells to gen-...
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Báo cáo khoa học: Ets-1/ Elk-1 is a critical mediator of dipeptidyl-peptidase III transcription in human glioblastoma cells pdf
Ngày tải lên :
29/03/2014, 09:20
... observed that expression of DPP-III mRNA in CLR cells was twofold higher than in CLDR cells (P £ 0.002) Treatment of CLR cells with U0126 decreased the DPP-III mRNA level by threefold (P £ 0.03), ... (CLR) cells exhibited approximately twofold higher levels of pElk-1 (P £ 0.002) compared with cells stably transfected with the empty vector (Chang liver DRas; CLDR) (Fig 8A) Transfection of pAAS-1 ... fibroblast) cells After 48 h of transfection, cells were washed three times with ice-cold NaCl ⁄ Pi, lysed and luciferase activity was assayed in the cell lysates Cells transfected with pGL3-Basic...
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