epidermidis and e coli
PHOTOREACTIVATION OF ENTEROHEMORRHAGIC E. COLI, VRE AND P. AERUGINOSA FOLLOWING UV DISINFECTION
Ngày tải lên :
05/09/2013, 08:40
... may also be affected by water transparency, and the e ect may be estimated by similar method used for estimation of UV dose decreases in water The difference between inactivation and photoreactivation ... e ect of water transparency on UV disinfection e ciency This minus e ect is usually estimated by considering UV dose decreases in the target water UV dose decreases are estimated by determining ... suspended about 15 cm above the liquid surface A beaker containing the UV-treated bacterial suspension mixed with a magnet bar was placed on a magnetic stirrer Samples were taken after timed intervals...
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E. coli Shiga Toxin Methods and Protocols doc
Ngày tải lên :
23/03/2014, 07:20
... Oligonucleotide Probes Access to cloned stx and stx genes and their respective nucleotide sequences enabled the development of DNA and oligonucleotide probes for the detection of STEC (reviewed in ref ... STEC-related disease can be particularly problematic when patients present late in the course of disease, because the numbers of STEC in feces may be extremely low and hence undetectable even ... (Stx) in the gut lumen have been described (1,2), and these are likely to be most effective when adminstered early in the course of disease, before serious systemic sequelae develop Also, the clinical...
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Báo cáo khoa học: Alternative substrates for wild-type and L109A E. coli CTP synthases Kinetic evidence for a constricted ammonia tunnel doc
Ngày tải lên :
23/03/2014, 13:20
... of these molecules Results and discussion Previously, we reported that replacement of the highly conserved Leu109 residue in E coli CTPS by alanine yields an enzyme that has kinetic properties ... utilize alternative substrates We show that replacement of Leu109 by alanine in E coli CTPS causes the enzyme to discriminate between nascent NH3 and the bulkier analogue NH2OH based on size but ... (see above) The ionic strength was maintained at 0.30 M in all assays by the addition of KCl All kinetic parameters were determined in triplicate and average values are reported The reported errors...
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Báo cáo khoa học: Redox-sensitive loops D and E regulate NADP(H) binding in domain III and domain I–domain III interactions in proton-translocating Escherichia coli transhydrogenase potx
Ngày tải lên :
31/03/2014, 09:20
... regarding important residues involved in the ecI–ecIII interface, the results revealed unexpected redox-dependent changes of the ecI–ecIII interface suggested to be relevant for the overall reaction ... be able to react these with thiol-specific reagents, all selected residues were mutated to cysteines in the cysteine-free E coli transhydrogenase Reverse reaction DISCUSSION Forward reaction were ... mutants were estimated from curves obtained when monitoring the decrease in fluorescence intensity as ecIII enzymes were treated with glutathione and glutathione reductase (see Materials and methods)...
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e. coli, shiga toxin methods and protocols
Ngày tải lên :
10/04/2014, 23:03
... putative STEC accessory virulence genes, such as eae and EHEC-hlyA have been described (5,6) These serve as markers for the locus of enterocyte effacement (LEE) pathogenicity island and From: Methods ... free Shiga toxin (Stx) in the gut lumen have been described (1,2), and these are likely to be most effective when adminstered early in the course of disease, before serious systemic sequelae develop ... laboratories Detection of stx Genes 3.1 Hybridization with DNA and Oligonucleotide Probes Access to cloned stx and stx genes and their respective nucleotide sequences enabled the development of DNA and...
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Báo cáo khoa học: " Pharmacokinetics and dosage regimen of ceftriaxone in E. coli lipopolysaccharide induced fever in buffalo calves" pot
Ngày tải lên :
07/08/2014, 18:21
... reweighted after selecting the model to obtain better estimates of kinetic parameters The dosage regimen of ceftriaxone was also determined based on the kinetic data The priming (D) and maintenance ... sample was diluted to the extent that its zone of inhibition came in linear range (preferably in the range of the zone of inhibition of the reference concentration) In this experiment, the reference ... to be most effective against the majority of sensitive Gram-positive and Gram-negative pathogens, the convenient and suitable dosage regimen of ceftriaxone in the febrile buffalo calves after...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
Ngày tải lên :
12/08/2014, 04:21
... Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and ... could be internalized by antigen presenting cells and presented to T and B cells There are some instances in which RTB has been used successfully as a carrier fused to other molecules For example, ... the chimeric protein retained heightened immunogenicity based on the adjuvant properties of the genetically linked RTB Indeed, the presence of RTB markedly enhanced immune responses in mice to p24...
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Báo cáo khoa học: "Transfer of immunoglobulins through the mammary endothelium and epithelium and in the local lymph node of cows during the initial response after intramammary challenge with E. coli endotoxin" ppt
Ngày tải lên :
12/08/2014, 18:22
... 50:26 experiment At the day before surgery, California Mastitis Test (CMT) results of left hind quarters were negative, and those of the other quarters were negative or showed the lowest degree of ... release and/ or selective mechanisms influencing the transfer However, these studies have not made it possible to investigate the transfer through each of the two barriers, the mammary endothelium ... over time when repeated sampling is desirable, it appears more suitable to examine interstitial fluid that can be sampled frequently after it has entered the collecting vessels of the peripheral...
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Báo cáo y học: "Dramatic increase of third-generation cephalosporin-resistant E. coli in German intensive care units: secular trends in antibiotic drug use and bacterial resistance, 2001 to 2008" pot
Ngày tải lên :
13/08/2014, 20:22
... imipenem and meropenem therapy [31] In contrast, the increase in 3GC-resistant E coli has now affected most ICUs and is therefore unlikely to be a problem caused by the selection pressure of ... pathogens [15] These routine data were reported to the National Reference Centre of the Surveillance of Nosocomial Infections Ethical approval and informed consent were thus not required Statistical ... corresponds to seven isolates from two separate ICUs Figure 4a, b shows the pooled mean and the median of carbapenem use and 3GC resistance in E coli Resistance started to increase in parallel...
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Báo cáo sinh học: "Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score" ppt
Ngày tải lên :
14/08/2014, 13:21
... The detailed selection strategy and phenotypes of selected heifers are described by Kühn et al [27] In brief, three sires were selected from the German Holstein population based on the discrepancy ... marker haplotypes and thus, indirectly, the inherited paternal QTL alleles were inferred, and eleven heifers were selected from the pool of daughters Six Brand et al Genetics Selection Evolution 2011, ... Dams and dam sires of the heifers were preselected for high (low susceptible heifers) and low relative estimated breeding values (high susceptible heifers) to increase the probability that the heifers...
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Immune modulation by experimental filarial infection and its impact on e coli induced sepsis
Ngày tải lên :
26/11/2015, 10:00
... one day before E coli challenge Macrophage depletion prevented the L.s.-mediated protective effect on sepsis Naùve and L.s.-infected animals developed a more severe hypothermia when injected with ... actively secreted molecules and molecules that are released passively at events like molting or release of microfilariae, all filaria-released molecules are in general termed excretory/secretory products ... insect vector These larvae migrate to species-specific sites (see below), where they molt twice into adult worms Female and male adult worms mate and females release first stage larvae, termed...
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Hoàn thiện quy trình biến nạp đoạn dna vào tế bào vi khuẩn E. coli DH5
Ngày tải lên :
27/10/2012, 10:53
... Site OD Optical Density PCR Polymerase Chain Reaction RE Restriction Enzyme RNA Ribonucleic acid RFLP Restriction fragment length polymorphism SDS Sodium dodecyl sulfate TAE Tris Acetate EDTA ... base pair CFU Colony Form Unit DNA Deoxyribonucleic acid dNTP 3’- Deoxyribonucleoside- 5’- triphosphate ETDA Ethylenediamine tetraacetic acid IPTG Isopropyl-β-D-thiogalactoside MCS Multiple cloning ... Taq Thermus aquaticus Kb kilobase dATP deoxyadenosine triphosphate dGTP deoxyguanosine triphosphate dCTP deoxycytidine triphosphate dTTP deoxythymidine triphosphate Tris- HCL (hydroxymethyl)...
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Thử nghiệm sản xuất kháng huyết thanh kháng vi khuẩn E. coli
Ngày tải lên :
29/10/2012, 14:14
... DANH SÁCH CÁC CHỮ VIẾT TẮT APC Antigen-presenting cell (Tế bào trình diện kháng nguyên) E coli Escherichia coli Ig Immunoglobulin (Globulin miễn) IL Interleukine KHT Kháng huyết KN Kháng nguyên ... lại nhƣ lipopolysaccharide Escherichia coli, flagellin Salmonella, vỏ polysaccharide vi khuẩn Pneumococcus, dextrans, levans polyglutamic acid Bởi chúng trình tự polimer lặp lại nên phân tử gắn ... 44oC Lên men đƣờng glucose, lactose, manitol, sorbitol, phản ứng -galactosidase dƣơng tính Indol dƣơng tính, methyl red dƣơng tính, Voges-Proskauer âm tính, citrate âm tính Không sử dụng phenylalanin,...
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Các phương pháp phát hiện E.Coli trong thực phẩm
Ngày tải lên :
30/10/2012, 10:39
... EIEC (Enteroinvasive E. coli) : E. coli xâm nhập ruột EAEC (Enteroaggregative E. coli) : E. coli ngưng tập ruột DAEC (Diffusely adherent E. coli) : E. coli bám dính phân tán EHEC (Enterohaemorrhagic E. coli) : ... (ExPEC-extraintestinal pathogenic E. coli) - Các loại E. coli gây bệnh đường ruột (IPEC) biết gồm: EPEC (Enteropathogenic E. coli) : E. coli gây bệnh đường ruột ETEC (Enterotoxigenic E. coli) : E. coli ... 47 [Type text] Page [Type text] [Type text] [Type text] Chương I: TỔNG QUAN VỀ VI KHUẨN ESCHERICHIA COLI I SƠ LƯỢC VỀ VI KHUẨN ESCHERICHIA COLI (E. COLI) Escherichia coli Theodore Escherich (1857-1911),...
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Xây dựng quy trình biến nạp đoạn DNA vào tế bào vi khuẩn E.coli DH5α
Ngày tải lên :
31/10/2012, 09:35
... axit nucleic cần cho vào môi trƣờng phản ứng mồi axit nucleic DNA polymerase I(từ E. coly) chuỗi polypeptide với ba hoạt động enzym: DNA polymerase (tổng hợp) 5’ exonuclease 5’ 3’, exonuclease (thủy ... hợp DNA polymerase 5’ 3’ hoạt động thủy giải exonuclease 3’ 5’ 2.5.2.2 Terminal transferase Enzym đƣợc ly trích từ tuyến ức bê Terminal transferase xúc tác phản ứng gắn deoxynucleotide vào đầu 3’OH ... động (I có promoter riêng) để tạo repressor; repressor nhận biết lyên kết với operator, cản lyên kết RNA polynerase promoter Khi có lactose (và glucose), lactose lyên kết với repressor làm biến...
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Nghiên cứu tìm kiếm vaccine hiệu quả cho việc phòng tiêu chảy trên heo sau cai sữa do E. coli (81 trang)
Ngày tải lên :
03/11/2012, 09:10
... them by IMViC test, then serotype with F4, F5, F6 antiserum Second, selecte E coli isolates of serotype F4 or F5 to produce the autovaccine Third, set up the experiment to evaluate the efficiency ... auto-vaccine (the experiment was repeated in times): The first group: litters (28 piglets, from 14 to 20 days aged), vaccine injection one times The second group: litters (28 piglets, from 14 ... auto-vaccine was evaluated by antiody titers, medium daily gain and the side effects Result: The auto-vaccine made a high antibody titers and long immunization The auto-vaccine did not bring neither the...
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Khảo sát tình hình nhiễm E.coli và Coliforms trong nước uống
Ngày tải lên :
06/11/2012, 09:47
... - EAEC (Enteroaggregative E coli) , E coli kết tập ruột - EHEC (Enterohemorrhagic E coli) , E coli gây xuất huyết ruột - EPEC (Enteropathogenic E coli) , E coli gây bệnh đƣờng ruột - ETEC (Enterotoxigenic ... Brilliant green agar BGBL : Brilliant Green Lactose Bile Salt CFU : Colony Forming Unit EMB: Eosin Methylene Blue E coli: Escherichia coli LT: Heat Labile ST: Heat stable MR-VP: Methyl Red- Voges Proskauer ... Coliforms khơng có nguồn gốc từ phân 2.4 Sơ lƣợc E coli 2.4.1 Đại cƣơng Vi khuẩn Escherichia coli (E coli) thuộc [11]: Lớp: Schgzomycetes Bộ: Eubacteriales Họ: Enterobacteriaceae Tộc 1: Escherichiae...
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Xác định vai trò gây bệnh của vi khuẩn E.coli , C.perfringens trong hội chứng tiêu chảy ở lợn từ sơ sinh đến 60 ngày
Ngày tải lên :
06/11/2012, 11:22
... Adherence Enteropathogenic Escherichia coli Brain-heart infusion Cng s Colonial Forming Unit Edema disease Edema disease pathogenic Enterohaemorrhagic Escherichia coli Eosin Methylene Blue Agar ... Agar Enteropathogenic Escherichia coli Enterotoxigenic Escherichia coli Heamolysin Heamolysin Khỏng nguyờn Heat-Labile enterotoxin Nh xut bn Polymerase Chain Reaction Shiga-like toxin Shiga-like ... Enteropathogenic E coli (EPEC), Adherence Enteropathogenic E coli (AEEC) v Verotoxingenic E coli (VTEC) (Lờ Vn To, 1997a [48]) Trong ú, cỏc chng vi khun thuc nhúm ETEC v VTEC thng gõy bnh tiờu...
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