enzymology of microsomal glutathione s transferase

... mass of some yeast (Y lipolytica) GSTs, however, is 110 kDa [32] Thus GSTs can vary widely in their subunit size The larger molecular mass of turtle GSTs may arise as a result of post-translational ... lower specific activities of GSTs in liver from anoxic turtles (using either CDNB or cumeme hydroperoxide as substrates) suggest a possible specific suppression of GST activity during anaerobiosis, ... directed against thiol groups, is a nonspecific inhibitor of all classes S- hexylglutathione shows highest to lowest inhibition of a, l and p GST isozymes, respectively [38] Both Peak and GSTs showed...

Ngày tải lên: 23/03/2014, 15:20

... conditions as given in Fig Ó FEBS 2002 Purification of R decussatus GST isoforms (Eur J Biochem 269) 4363 Table Biochemical characteristics of R decussatus GST isoforms Results are expressed as means ... class consensus sequences from mammals, respectively The N-terminal sequence of subunit of R decussatus exhibits a high similarity with the corresponding consensus mu GST class from mammals DISCUSSION ... antisera specific of mammalian pi-class GST Studies of aquatic species such as salmonids [26], Bufo bufo [27] and M edulis [28], showed that their GSTs belong to the pi class The GST isoforms 1-1...

Ngày tải lên: 31/03/2014, 09:20

... regions of SmGSTO presented significant divergences from the known GSTO, at the primary structure level Indeed, the GSTO class represents a particular class of the GST superfamily which possesses specific ... Construction of a homology model of SmGSTO SmGSTO structure was built using the human GSTO 1–1 structure as template in the SWISS-MODEL modeling Ó FEBS 2002 Omega-class GST from Schistosoma mansoni ... blot analysis was performed using S mansoni cercariae purified DNA as described [26] Alignments and phylogenetic analysis of S mansoni GSTs Sm26GST, Sm28GST and SmGSTO amino acid sequences were aligned...

Ngày tải lên: 21/02/2014, 01:21

... GSTU2 This assumption is also supported by the analysis of gene expression, which showed that the GSTU1 gene is induced by cadmium sulfate, CDNB, cyhalothrin, and cold stress, whereas GSTU2 is ... that observed for GSTU2 [11] In the present work, sitedirected mutagenesis was used to evaluate the effects of the single mismatched residues on the kinetic properties of isoforms GSTU1 and GSTU2, ... engineered GSTs showing altered substrate specificity and enhanced activity towards xenobiotics Results and Discussion Wild-type (GSTU1 and GSTU2) and mutant GSTs were expressed and purified as described...

Ngày tải lên: 15/03/2014, 09:20

... at least six classes of cytosolic GSTs in insects [2] The majority of GSTs are in the Delta and Epsilon classes, and the remaining enzymes are in the Omega, Sigma, Theta and Zeta classes The ... specificities of purified cockroach GST compared with those of other insect GSTs Values are the means ± SE from three separate experiments Substrate specificities of Delta class GSTs from D melanogaster ... Peroxidase activities of GSTs are of particular importance to insects, because they not possess selenium-dependent glutathione peroxidase A survey of reported GST-mediated peroxidase activity across...

Ngày tải lên: 23/03/2014, 09:21

... plates Wells containing single cells were marked, and growth in these wells was monitored daily Expression of hGSTA4-4 protein was ascertained by Western blot analysis Site-directed mutagenesis of ... cells with antibodies to cleaved caspase-3 (data not shown) and loss of fluorescence due to extrusion of cytoplasm These cells could be easily distinguished from the rounded, transformed cells As ... with hGSTA4-4 into smaller rounded immortalized cells which grow rapidly in suspension is surprising but it seems to be consistent with numerous previous studies suggesting that 4-HNE is involved...

Ngày tải lên: 30/03/2014, 13:20

... cells With this assay, we determined the effectiveness of a set of antisense PS-ODNs directed against the glutathione S- transferase Mu1 (GSTM1) and Mu2 (GSTM2) isoforms of the rat GSTs play an important ... tested AS-ODNs) DISCUSSION Most of currently available screening assays for the selection of effective AS-ODNs are based on cell-free assays, e.g RNAse H digestion screens and oligonucleotide scanning ... C-terminal fusion constructs The newly developed screening assay was used for the selection of effective AS-ODNs against rat GSTM1 and GSTM2 out of a set of 15 PS-ODNs Some ODNs were designed to specifically...

Ngày tải lên: 31/03/2014, 15:20

... apoptotic response of chondrocytes to HNE is associated with decreased Bcl-2 expression and increased Bax expression This suggests that chondrocytes need to modulate the synthesis of at least several ... milligram of proteins Statistical analysis Results were expressed as the mean ± standard error of the mean of eight specimens, and assays were performed in three independent experiments Statistical ... promote GSH/GSSG exchange It is, however, also possible that HNE decreased the GSH pool through inhibition of GSH synthesis In any case, pre-treatment of cells with antioxidants, such as cysteine,...

Ngày tải lên: 09/08/2014, 13:21

... association of GSTs with the risk of urothelial carcinoma according to various cutoff points of arsenic exposure level List of abbreviations As: arsenic; GST: glutathione S- transferase; GSTO: Glutathione ... risk, respectively in one hospital-based casecontrol study [33] A study in Bangladesh also showed an joint effect of arsenic exposure with GSTOs on Asinduced skin lesions [20] Based on these ... association of GSTO1 with urinary arsenic metabolite pattern remains inconclusive because several studies did not show a significant association [28-30] The inconsistent results among these studies...

Ngày tải lên: 10/08/2014, 05:21

... bands from the tissues but not from sputum supernatants These results can be related to many reasons one of those being proteolysis of GST alpha in the tissues Another, even more likely reason is ... Statistical methods The statistical analyses were performed with the SPSS for Windows software (SPSS, Chicago, IL, USA) Continuous data were compared using analysis of variance (ANOVA) http://respiratory-research.com/content/9/1/80 ... males irrespective of smoking [18] Most of these studies on GST polymorphisms highlight the importance of these enzymes in protection against the oxidative stress induced by cigarette smoke This...

Ngày tải lên: 12/08/2014, 14:20

... moderate/intense in 8/16 non-smokers, 9/22 Statistical methods The statistical analyses were performed with the SPSS for Windows software (SPSS, Chicago, IL, USA) Continuous data were compared using analysis ... (not shown) Western blot analysis for GSTO1-1 showed decreased levels of GSTO11 in the supernatants of COPD patients compared to nonsmokers (p = 0.023) (Figure 5A) These results suggest that GSTO1-1 ... Figure cigarette role of GSTO oxidative stress Suggested role of GSTO (glutathione transferase omega) in cigarette smoke induced oxidative stress Enzymes maintaining GSH homeostasis are important in...

Ngày tải lên: 12/08/2014, 15:20

... analyses stratified by SE dose (0, or copies) rather than SE positivity, there were marked differences in the associations of composite risk factors of GSTM1 status and SE dose with ACPA positivity ... positivity were also examined in separate analyses Patients were then categorized on the basis of the presence of risk factor pairings (GSTM1-SE, GSTM1smoking and smoking-SE), and associations ... to ACPA-positive disease and not apply to seronegative disease [2] Because of the limited sample sizes in subgroups of interest, our study did not include analyses of interactions of distinct HLA-DRB1...

Ngày tải lên: 12/08/2014, 15:22

... Thạc s Nuôi trồng thủy s n Đại học Cần Thơ Edwards, C.A., Fisher, S. W., 1991 The use of cholinesterase measurement in assessing the impact of pesticides on terrestial and aquatic invertebrates ... Richardson, R.J., Saunders, D.R., Sheets, L.P., Sultatos, L.G , Wallance K.B., 1998 Common mechanism of toxicity: A case study of organophosphorus pesticides J Toxic Sci 41, 8-20 Murty, A .S. , 1988 ... Modra, M Slais , Z Svobodova Effects of subchronic exposure to Successor® 600 on common carp Cyprinus carpio Neuroendocrinology Letters Volume 30 Suppl 2009, p 230-235 Koesomadinata, S and R Djajadiredja.,...

Ngày tải lên: 11/03/2014, 05:21

... change of -0.8 ml/yr We assessed the impact of genetic GST deficiency on lung function decline sepa- rately for never smokers and persistent smokers; associations observed in ever-smokers were similar ... ever smokers), as well as BHR and age, was assessed by including according multiplicative interaction terms in the regression models Trend tests for the combination of GSTT1 and GSTM1 genotypes ... negative subjects No association of GST genotype with FVC was observed in male BHR positive subjects In BHR positive women again no statistically significant GST genotype/lung function associations...

Ngày tải lên: 12/08/2014, 16:20

... analysis 49 2.10 Statistical analysis 50 RESULTS 51 3.1 Glutathione S- transferase pi (GST-pi) expression 52 3.2 Total glutathione S- transferase (GST) activity 55 v 3.3 Bcl-2 expression 59 3.4 Association ... adaptations allows it to modulate the severity of the onslaught and survive Introduction 30 Figure Defence against apoptosis 1.7 Glutathione S- transferase pi (GST-pi) Glutathione S- transferases (GSTs) ... angiogenesis; and (6) tissue invasion and metastasis (Hanahan et al., 2000) Acquisition of the “hallmark capabilities” sets the scene for tumour expansion Apoptosis is a process of physiologically...

Ngày tải lên: 16/09/2015, 17:14

... discussion The Law has been translated into English by several translators An assessment of the English version of this law is thus desirable 2 Significance of the study The results of this study ... House s model for translation quality assessment in assessing English translations of Vietnamese legal documents This is done through the application of the model in assessing the English version of ... different views on equivalence will be discussed but there shall be no separate discussion of translation assessment; instead, it is implied that assessment of a translation is based on the respective...

Ngày tải lên: 07/11/2012, 14:36

... kết thúc Ví dụ: "fax" kết thúc chữ "x" âm /s/ "like" kết thúc chữ "e" âm /k/Ngoại lệ: s từ kết thúc -ed dùng làm tính từ phát âm /Id/: * aged * blessed * crooked * dogged * learned * naked *...

Ngày tải lên: 18/09/2013, 06:10

... Parkinson s Disease Parkinson s disease (also known as Parkinson disease or PD) is a degenerative disorder of the central nervous system that often impairs the sufferer s motor skills and speech ... minimum of bias and confounding Systematic reviews summarize the existing evidence from groups of RCTs Assessing the methodologic quality of primary studies refers to aspects of study design, performance, ... patients with chronic depression (Linde et al., 2005a) However, extracts of St John s wort had similar effectiveness as standard anti-depressants, such as selective serotonin reuptake inhibitors (SSRIs)...

Ngày tải lên: 25/10/2013, 05:20

... INTRODUCTION of Some Linguistic Features of Barack Obama s English Speeches” 1.2 AIMS AND OBJECTIVES 1.2.1 Aims This study aims at analyzing the stylistic features of Barack Obama s English speeches (ESs) ... features of Barack Obama s speeches In addition, descriptive method is used to give a detailed description of some stylistic features of Obama s speeches 3.2 DESCRIPTION OF SAMPLES All sources of ... Microsoft words… 3.4 DATA COLLECTION This research is restricted to most common-used stylistic devices in Obama s speeches Data for this research are collected from different types of speeches of...

Ngày tải lên: 26/11/2013, 13:21