enough to pack but not too much

... to increase pH to a value of 6.25 (A) Log of fluorescence intensities corresponding to tryptophan emission (j) or Oregon green emission (h) is plotted vs pH (B) The maximum wavelength of tryptophan ... chromatography on a PD10 column (Pharmacia) in 0.1 M NaHCO3 buffer pH 8.6 Immunological techniques The ELISA technique [32], was used to monitor interaction of gelsolin domains, peptides to gelsolin ... disrupts interdomain bonds but not those formed through the G2–6 interface Methods Proteins and peptides Rabbit skeletal muscle actin was isolated from acetone powder [23] and stored in buffer G (2...

Ngày tải lên: 23/03/2014, 21:20

... of Tanzania] Ideally, countries with resources too limited to expand care to all patients with CD4 counts of less than 350 cells/mm3 would be able to identify and target other atrisk populations ... chose not to include weight in these initial models as previous studies suggested that the inclusion of weight in the Cockcroft-Gault formula, but not in the simplified MDRD formula, led to significant ... disease): and (2) time to overall mortality We specifically chose to use times to meeting criteria for starting cART, rather than actual times to starting cART, as treatment may not have been initiated...

Ngày tải lên: 20/06/2014, 08:20

... is not well understood Specifically, the rare combinations of mutations have not been analyzed in depth As novel nucleoside reverse transcriptase inhibitors (NRTI) continue to evolve and be employed ... was calculated according to our previously published protocols [14] To confirm the ratios of peak heights observed, we performed population cloning in Topo TA cloning vector PCRR2.1 (Carlsbad, ... background of K65R leads to an RT which is much more stable as compared to RT with the K65R+L74V mutations In order to validate the reversion observed in sequence-chromatograms, we performed population...

Ngày tải lên: 11/08/2014, 21:21

... makes little contribution to Newtonian resistance in mice and thus R ≈ Raw [33] Page of 18 (page number not for citation purposes) Respiratory Research 2005, 6:142 http://respiratory-research.com/content/6/1/142 ... order to characterise the primary inflammatory and cytokine response to RSV infection, we chose the appropriate kit to measure innate immune responses This included tumour necrosis factor alpha ... significant in adult mice at d (p < 0.05), but did not reach significance in weanling mice Cytokines and mediators in BALF Cytometric bead assay IL-12 p70 was not detectable in BALF from adult mice...

Ngày tải lên: 12/08/2014, 18:20

... histones with variant histones Steve Henikoff (Fred Hutchinson Cancer Center, Seattle, USA) used chromatin-affinity purification of histone variant H3.3 in combination with tiling microarrays to ... factors share about 20% of their respective sets of target genes, including many Hox genes Genes bound by all three factors can be active or repressed Jaenisch suggested that an autoregulatory ... factor and epidermal growth factor these precursors can be expanded into neural stem cells that no longer express Sox1 and that can be propagated indefinitely without losing their potential to...

Ngày tải lên: 14/08/2014, 16:20

... genotype seems to induce apoptosis with faster kinetics and to suppress transformation more efficiently than the Pro/Pro genotype.[15] It was shown that p53 Pro/Pro exhibits a lower ability to ... were not enough report support us to make meta-analysis in current research Studies with a larger sample size, especially including smoking or another environment factors will be helpful to confirm ... susceptibility factor The p53 tumor suppressor pathway is well-known to be crucial in maintaining genomic integrity and preventing cells from oncogenic transformation When a cell is exposed to genotoxic...

Ngày tải lên: 25/10/2012, 11:40

... were more polite than today  …………………………………………………………………… It is colder today than it was yesterday …………………………………………………………………… Our hotel was cheaper than all the others in the town ………………………………………………………………………...

Ngày tải lên: 25/09/2013, 22:10

... symmetryequivalent contributors) is not the most useful indicator, because it does not take into account the multiplicity of measurements More elaborate versions of Rmerge have been proposed [32,33], but they ... is not unusual to see R-factors of 1–2% An important parameter that was introduced into crystallographic practice in 1992 is free R [41] Rfree is calculated analogously to normal R-factor, but ... percent Moreover, if H atoms are not included, their contribution is represented completely by the parent atom and its position tends to refine to the ‘center of gravity’ of both atoms As a result the...

Ngày tải lên: 16/03/2014, 06:20

... epitope but not the T cell epitopes may avoid a deleterious cellular immune response Prior reports have suggest that this may be true as shorter Aβ fragments conjugated to T cell helper epitopes ... and applied to Aβ1–40-coated ELISA plates Immunohistochemistry and image analysis Immunohistochemistry was performed on 12 µm paraffin sections using the ELITE ABC method (Vector Laboratories, Burlingame, ... peptide in a novel manner The use of the adjuvant LT(R192G) was not enough to overcome tolerance as seen with the lack of a immune response to Aβ1–15 peptide Therefore, we constructed a novel immunogen,...

Ngày tải lên: 19/06/2014, 22:20

... NP40, 100 mM KCl, mM dithioerythritol, mM EDTA) containing a mixture of protease inhibitors IgG antibodies from sera to be analyzed (SjS and controls) were coupled to protein A-agarose beads (Biozym, ... of apoptotic extracts (to increase the amount of antigenic fodrin cleavage product) did not improve the suitability of these techniques for the detection of anti-α-fodrin antibodies (data not shown) ... and anti-La autoantibodies have been reported to be 60–75% and 30–50%, respectively [6–9], while the specificity of particularly anti-La autoantibodies is generally considered to be reasonably...

Ngày tải lên: 09/08/2014, 01:23

... inhibitors (NRTIs) being a staple of most regimens [3,4] In addition to NRTIs, which are both competitive inhibitors and chain-terminators, the nonnucleoside reverse transcriptase inhibitors (NNRTIs) ... HIV-1 RT shown to confer resistance to AZT (T215Y/M41L) and ddI and ddC (L74V) were sensitive to inhibition by RT1t49 (Table 3) In contrast, mutations shown to confer resistance to multiple NRTIs, ... The observed defect in Figure appears to be due to a loss in substrate affinity and not due to defect in the RNase H catalytic activity of these mutant RTs To determine whether these mutant RTs...

Ngày tải lên: 10/08/2014, 05:20

... number not for citation purposes) Results U83A efficiently displaces human chemokines from CCR5 signalling chemokine receptor in model system to compare to regulatory receptors In order to investigate ... activities on regulatory receptors and to compare them to those on signalling receptors, U83A binding competition was screened against chemokines also relevant for regulatory receptor specificity ... cascades leading to cell polarisation, cytoskeletal changes, and chemotaxis We have shown previously that U83A is able to block signalling receptor function by stopping their endocytosis via clathrin...

Ngày tải lên: 11/08/2014, 08:22

... possible Variables contributing to the recovery of mental health To determine the variables that contributed to the recovery from depression, anxiety and PTSD symptomatology for the physically/psychologically ... primary factors In contrast, cessation of physical IPV was not a significant predictor of recovery from anxiety Finally, the variables that contributed significantly to the change in PTSD symptoms ... understanding the factors that contribute to women’s responses to IPV that allow them to become free of the violence is of relevant importance Some studies have started to assess this issue [33,67,68],...

Ngày tải lên: 11/08/2014, 16:22

... throw myself into an area where it's impossible to get heroin The best I can is one day without There use an element of control I suppose, but it's not enough to break free I don't want to die Either ... want to die, but it doesn't make me not want to use If it did I wouldn't use any more, because I've dropped a few times It hasn't frightened me off enough I know if I die, I'll just go to sleep ... to talk freely of their experiences and opinions General discussion topics of interest were listed on a checklist to ensure all interviewees views were sought on each topic Discussions were not...

Ngày tải lên: 11/08/2014, 18:20

... from the tomato Sgt1 gene (TC85297) identified a potato EST, TC112395 (1461 bp), which showed 98% sequence similarity to the tobacco and tomato Sgt1 genes and 90% sequence similarity to the Arabidopsis ... (TIGR) potato database [26] using a sequence from the tobacco Rar1 gene (AF480487) identified a potato EST, TC121848 (1187 bp), which showed 96% sequence similarity to the tobacco and tomato Rar1 ... particle" tool in the ImageJ software [49] This tool scans the image selection until it finds the edge of an object, corresponding to fluorescing sporangia or mycelium on the leaf surface The tool...

Ngày tải lên: 12/08/2014, 05:20

... the autoimmune but not inflammatory response to cartilage type II collagen leading to chronic arthritis development Arthritis Research & Therapy 2011 13:R54 Submit your next manuscript to BioMed ... synthetic cysteine protease inhibitors are believed to have a protease inhibitory spectrum similar to that of cystatin C These studies, however, were not able to directly assess the physiological ... development of an autoimmune B and T cell response to type II collagen, which in turn leads to the early development of a chronic CIA, rather than having an effect on the inflammatory effector phase Our...

Ngày tải lên: 12/08/2014, 15:22

... in DAS28 rheumatoid arthritis remission but doi:10.1186/ar3393 Cite this article as: Yoshida K, et al.: Response to ‘Pain persists in DAS28 rheumatoid arthritis remission but not in ACR/EULAR ... Tokyo, 104-8560, Japan 3Division of Allergy and Rheumatology, St Luke’s International Hospital, 9-1 Akashicho, Chuo-ku, Tokyo, 104-8560, Japan not in ACR/EULAR remission: a longitudinal observational ... in patients with rheumatoid arthritis, and comparison with the DAS28 based on erythrocyte sedimentation rate Ann Rheum Dis 2009, 68:954-960 Inoue E, Yamanaka H, Hara M, Tomatsu T, Kamatani N:...

Ngày tải lên: 12/08/2014, 17:22

... affinity to ConA or DS lectin due to the lack of oligosaccharide modification of this BST-2 mutant Glycosylation of BST-2 is not required for inhibition of virus release and sensitivity to Vpu To assess ... sensitive to Vpu to varying degrees (Fig 6) In particular the C13 and C3A mutants were highly sensitive to Vpu similar to wt BST-2 while expression of the C12 and C23 mutants appeared to be less ... bifunctional crosslinker to physically link otherwise fully detached virions to the cell surface or to each other [24] Such a mechanism is very attractive but would be difficult to explain without...

Ngày tải lên: 12/08/2014, 23:22

... does not bind to the viral Env protein, but to a cellular factor incorporated into the viral envelope For viruses produced in the kidney-derived cell line 293T, this factor was Page of 18 found to ... that CLEC-2, in contrast to DC-SIGN, does not capture HIV-1 Env Instead, CLEC-2 seemed to recognize a cellular factor expressed on 293T cells, and binding to this factor did not depend on recognition ... interaction was not inhibited by mannan and was not enhanced by expression of the viral Env protein In agreement with these results, soluble HIV-1 Env protein bound specifically to DC-SIGN but not to CLEC-2...

Ngày tải lên: 12/08/2014, 23:23

... with an increased sensitivity to PRO 542 but decreased sensitivity to Q4120 and BMS378806 HIV-1 R5 macrophage-tropism also correlated with sensitivity to 2G12 but not with b12, TAK779, SCH350581 ... was reported to inhibit gp120 binding to CD4 [39-41] and subsequent conformational changes [42] BMS378806 is believed to bind into a deep hydrophobic channel of unliganded gp120 close to and underneath ... sensitivity to 2G12 Summary of correlations between macrophage-tropism and sensitivity to inhibitors Table and Figure show that R5 macrophage-tropism correlates with sensitivity to inhibitors that...

Ngày tải lên: 13/08/2014, 06:20