engineering requirements for reliable microdrop generation

Ngày tải lên: 16/10/2014, 14:08

Ngày tải lên: 28/06/2014, 09:20

... genealogic factors, and environmental components form the basis of the pediatric environmental history and are shown here Page of (page number not for citation purposes) Journal of Medical Case ... malformations caused by failed mesenchymal development during the first trimester of gestation CBEEC is a continuum, representing different levels of severity within the same spectrum [10] Before ... rare pediatric diseases However, it is not sufficient to meet the Bradford–Hill causal criteria The individual risk assessment for these patients is a complex process that requires specific diagnostic...

Ngày tải lên: 11/08/2014, 17:21

... children under the age of 17 were exposed to household ETS [15], and therefore an estimated 3.3 million children were at risk for adverse health effects from this exposure Evidence of the effects ... visit emergency rooms or ever hospitalized Early-onset asthma was defined as age of onset for asthma before years of age Late-onset asthma was onset after years of age Wheeze was defined as any ... child’s chest sounding wheezy or whistling Current wheeze was defined as wheezing for or more days out of the week for a month or longer in the previous year Bronchitis status was positive if subjects...

Ngày tải lên: 12/08/2014, 11:20

... Figure and PAR2 Effects of agonists for protease activated receptor (PAR)1 Effects of agonists for protease activated receptor (PAR)1 and PAR2 The agonists used for PAR-1 and PAR-2 are NH2S-F-L-L-R-N-C ... have fully active purified Der p1 was reduced by incubating it with 0.5 mmol glutathione for minutes before it was applied to the cell cultures Chymostatin (10 µg/ml, Sigma) was used as non-specific ... inhibitors were incubated for 15 minutes (37°C) with HDM, Der p and Der p containing medium Heat-treatment of media containing HDM extract, Der p and Der p was done at 65°C for 30 minutes After 24...

Ngày tải lên: 13/08/2014, 13:22

... which explained the apparent contradiction mentioned before Among genes with Ka/Ks < 0.06, the average dSM was 0.72 for HK genes, but 0.65 for non-HK genes Not surprisingly, we found that the previously ... explanation for the differences in promoter sequence conservation between genes that perform basic housekeeping functions, and which are simply regulated, and genes that are important for tissue- ... non-HK than in HK genes the differences were not significant (p > 0.64) Therefore, we con- Genome Biology 2007, 8:R140 information was only 23% (dSM = 0.77) On the other hand, many classes under-represented...

Ngày tải lên: 14/08/2014, 07:22

... WSSV 22 Viện NC & PT Công nghệ Sinh học Báo cáo Virus học đại cương - Có thể áp dụng kỹ thuật sốc formol 150-200ppm 30 phút đàn Poslarvae trước đưa vào nuôi, để loại cá thể yếu mang mầm bệnh - Cần ... mang mầm bệnh virus WSSV (cua, còng, tôm tự nhiên…) Hoặc dùng loại thuốc sát trùng mạnh (Nevugon, formol, chlorine…) tẩy dọn ao, làm khả cảm nhiễm virus tự nước - Chọn mùa vụ nuôi thích hợp để nuôi ... Đối với ao tôm bệnh, nên vớt tôm chết khỏi ao Sau dùng Chlorine với liều lượng 30 kg/1.000 m3; formol 200 lít/1.000 m3 hòa nước tạt xuống ao, ngâm ngày xổ môi trường Khi phát bệnh tốt thu hoạch...

Ngày tải lên: 22/01/2016, 00:57

... (5'-TAGTAGTATGCTCCGRGAA-3') for M and L segment amplifications, respectively The M segment RT reaction was performed at 50°C for 30 minute and PCR was performed at 94°C for min, followed by 40 cycles of 94°C for 15 ... by 40 cycles of 94°C for 15 sec, 50°C for 30 sec, 68°C for min, and a final extension at 68°C for L segment reactions utilized identical conditions, except for minute 68°C extension times The amplified ... thankful to Drs Thomas Ksiazek and Pierre Rollin for their help and support during the work Special thanks to Angela Sanchez for helping to perform phylogenetic analysis and valuable suggestions...

Ngày tải lên: 18/06/2014, 18:20

... cycle conditions: 50°C for minutes, 95°C for minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds Data analysis The ΔΔCT [23] method was used for initial data analysis ... recommendations for oligo(dT)20 primed cDNA-synthesis cDNA synthesis from RNA was performed using μg of RNA, at 50°C The cDNA was then treated with RNase H (Invitrogen) and diluted 1:100 before use for QPCR ... the most reliable reference gene across all infections However in contrast to their finding that TBP was equally reliable as PP1A we found it to be the 4th most reliable gene Other reliable genes...

Ngày tải lên: 18/06/2014, 18:20

... (5'-TAGTAGTATGCTCCGRGAA-3') for M and L segment amplifications, respectively The M segment RT reaction was performed at 50°C for 30 minute and PCR was performed at 94°C for min, followed by 40 cycles of 94°C for 15 ... by 40 cycles of 94°C for 15 sec, 50°C for 30 sec, 68°C for min, and a final extension at 68°C for L segment reactions utilized identical conditions, except for minute 68°C extension times The amplified ... thankful to Drs Thomas Ksiazek and Pierre Rollin for their help and support during the work Special thanks to Angela Sanchez for helping to perform phylogenetic analysis and valuable suggestions...

Ngày tải lên: 20/06/2014, 01:20

... cycle conditions: 50°C for minutes, 95°C for minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds Data analysis The ΔΔCT [23] method was used for initial data analysis ... recommendations for oligo(dT)20 primed cDNA-synthesis cDNA synthesis from RNA was performed using μg of RNA, at 50°C The cDNA was then treated with RNase H (Invitrogen) and diluted 1:100 before use for QPCR ... the most reliable reference gene across all infections However in contrast to their finding that TBP was equally reliable as PP1A we found it to be the 4th most reliable gene Other reliable genes...

Ngày tải lên: 20/06/2014, 01:20

... probes for EBNA1, EBNA2, and BXLF2/gp85 and two probes for LMP2 designed with either PE or AD probe design software All probes for EBNA1 had good sensitivity and specificity, and the probes for ... The levels for Raji, Jijoye and P3HR1 were the same as in B95.8 Notably, transcription values for B95.8 were close to saturation, and therefore, the ratios appear especially compressed for LMP2 ... ranged from 0.17 for ACTB to 2.24 for CLU Probes with a CV > 0.5 were eliminated Probes with a mean transcription signal > two SD and significant transcription in B95.8 were selected for the normalization...

Ngày tải lên: 20/06/2014, 01:20

... was performed on viral RNA using the Qiagen One-Step RT-PCR Kit with using 0.6 μM primer concentrations The pyrosequencing was performed as previously described [14] The primers used for PCR ... concentrations in parentheses): A/H1-forward GGAATAGC CCCCCTACAATTG (1 μM); A/H1-reverse AATTCGCATTCTGGGTTT CCTA (1 μM); A/H1 probe NED-CGTTGCC-GGATGGAMGBNFQ (0.05 μM); A/H3-forward CCTTTTTG TTGAACGCA-GCAA ... [7,13] Reverse transcription plates were set up for subtyping using a Qiagility robot (Qiagen, West Sussex, UK), and multiplex real-time PCR was performed using a Taqman Fast Universal PCR Master...

Ngày tải lên: 12/08/2014, 04:20

... reassortants of each of the three strains for the 2009-2010 season: A/California/7/2009 (H1N1), A/Perth/16/2009 (H3N2), and B/Brisbane/60/2008 Time post vaccination for subjects was 2-20 months Past ... statistically significant for all comparisons The degree of association between these measures was assessed using Pearson’s correlations Statistical analyses were performed using SPSS for Windows, version ... vaccination is recommended for people with increased rate of medical conditions (50-64 y/o), and for children months and older [21, 25] In some countries, vaccination is recommended for health care workers,...

Ngày tải lên: 25/10/2012, 11:10

... more motivated patients with hepatitis C absolutely deserve the extra means available for weight loss, not only for cardiovascular health but also to reduce the risk of fibrosis and ultimately cirrhosis ... genotype infections who have a form of steatosis that is directly induced by the hepatitis C virus and which also resolves with successful treatment These two forms of steatosis can certainly ... steatosis Two forms of steatosis exist in our patients with HCV The first type of steatosis occurs secondary to metabolic factors namely alcohol use or the metabolic syndrome This form of steatosis...

Ngày tải lên: 02/11/2012, 09:51

... patients Among 41 patients with persistent ALT elevation, 26 were seropositive for anti-HCV Of those seropositive for anti-HCV, serum HBVDNA was not detectable, and serum HCVRNA was detected in ... interferon three times a week for months Thirty patients with HBV-HCV coinfection, and chronic hepatitis were randomized to receive either or MU alpha-interferon three times a week for months Five patients ... interferon-alpha (IFN-alpha) and ribavirin combination therapy for patients with chronic hepatitis C and B virus (HCV/HBV) coinfection Forty-two chronic HCV/HBVcoinfected patients (29 IFN-naive,...

Ngày tải lên: 02/11/2012, 09:51

... (sought for with a sensitive technique, detecting 50 IU/ml or less) are present [3, 15] Detectable HCV replication in the absence of anti-HCV antibodies is exceptional with the current third -generation ... its foreseeable outcome, the presence of absolute or relative contra-indications to therapy, and the patient’s willingness to be treated The HCV genotype should be systematically determined before ... recommended in order to avoid treating for 48 weeks patients with no likelihood of an SVR [22, 23] In this purpose, HCV RNA quantification should be performed at baseline and after 12 weeks of...

Ngày tải lên: 02/11/2012, 09:56

... times higher than that of developed countries [49] Despite better screening for selecting blood donors, there remains a need for some kind of HCV screening laboratory test Sexual activity The role ... juvenile detention population carried HCV [69] The juvenile detention population therefore provides a target for teaching and intervention since many of these juveniles acquire the virus early ... counseling, HCV screening and substance abuse treatment In the US, the Centers for Disease Control (CDC) suggest screening for the follow population: • Persons who ever injected illegal drugs, including...

Ngày tải lên: 02/11/2012, 09:56

... months after exposure Up to 30% of patients will test negative for anti-HCV at onset of their symptoms, making anti-HCV testing unreliable in diagnosis of acute infection.[17] Almost all patients ... HCC, it is imperative to screen for HCC in HCV patients with cirrhosis In the U.S., Australia, and Europe, liver transplantation is the principle long-term treatment for patients with severely decompensated ... 2002 (June 10-12, 2002) Gastroenterology, 2002 123(6): 2082-99 Centers for Disease Control and Prevention Recommendations for prevention and control of hepatitis C virus (HCV) infection and HCV-related...

Ngày tải lên: 02/11/2012, 09:56

... as well as membranous web formation and RNA replication, illustrated here as separate steps for simplicity, may occur in a tightly coupled manner The replicon system For many years, HCV research ... were recently discovered For instance, the sequence coding for the C-terminal domain of NS5B consists of an essential stem-loop, designated SL3.2, within a larger cruciform RNA element, designated ... culture system Furthermore, the impact of viral proteins such as p7 and NS2 for viral particle formation and possibly of NS5A for the switch between replication and assembly can be explored in this...

Ngày tải lên: 02/11/2012, 10:00