endothelin 1 and nitric oxide biology

... .7 1. 6 .1 Types and Location 10 1. 6.2 Activation of SphKs 11 1. 6.3 Functions of SphK1 and S1P 12 1. 6.4 Clinical significances of sphingolipid pathways 15 1. 7 Aims and ... .18 1. 7 .1 Aims of this study .18 1. 7.2 Hypothesis .19 v Chapter 2: Materials and Methods 20 2 .1 Cell culture 21 2 .1. 1 Materials 21 2 .1. 2 Procedure ... factor β, 11 and insulin like growth factor -1; (b) Cytokines: TNF-α, interleukins; (c) Hormones: prolactin and estradiol; (d) Hypoxia, and (e) Histamine 1. 6.3 Functions of SphK1 and S1P The cellular...

Ngày tải lên: 16/10/2015, 15:38

... endotheliumderived relaxing factor and the chemical reactivity of nitric oxide J Exp Med 16 9, 10 11 10 20 Abu-Soud HM & Stuehr DJ (19 93) Electron transfer in the nitric- oxide synthases Nitric oxide synthases reveal ... radical within nitric- oxide synthase J Biol Chem 283, 11 734 11 742 35 Crane BR et al (19 98) Structure of nitric oxide synthase oxygenase dimer with pterin and substrate Science 279, 212 1– 212 6 36 Ghosh ... parameters: k1, k4 = 52 s )1; k8 = 28 s )1; k2, k5 = 10 00 s )1; k3, k6 = 200 s )1; k7 = 50 s )1; k9 = 0. 01 s )1; k10 = s )1 To improve signal to noise, spectra were averaged over intervals of 10 ms after...

Ngày tải lên: 07/03/2014, 00:20

... Biochem Pharmacol 38, 17 09– 17 15 Ó FEBS 2003 Arginine transport and NO formation in platelets (Eur J Biochem 270) 2 011 Mayer, B & Hemmens, B (19 97) Biosynthesis and action of nitric oxide in mammalian ... Ôarginine paradoxÕ J Biol Chem 272, 312 13– 312 16 39 Gross, S.S., Stuehr, D.J., Aisaka, K., Jaffe, E.A., Levi, R & Griffith, O.W (19 90) Macrophage and endothelial cell nitric oxide synthesis: cell-type selective ... Frolich, J.C (19 95) Supplementation of hypercholesterolaemic rabbits with L-arginine reduces the vascular release of superoxide anion and restores NO production Atherosclerosis 11 7, 273–284 11 Wang,...

Ngày tải lên: 08/03/2014, 02:20

... Behỗet /PHA B p< 0.0 01 300 250 p< 0.0 01 IL4 pg/ml 200 p< 0.0 01 p< 0.0 01 150 10 0 50 controls controls /PHA Behỗet Behỗet /PHA C 16 0 p< 0.0 01 140 p< 0.0 01 120 p< 0.0 01 p< 0.054 NO àM 10 0 80 60 40 20 ... Radic Biol Med 2007, 42:95570 11 Beckman JS , Koppenol WH: Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and ugly Amer J Physiol 19 96, 2 71: C1424C1437 12 Yu L, Gengro PE., Niedrberger ... in NCs: 11 34.02 83.70 versus 583.02 98.44 pg/mL, p

Ngày tải lên: 11/08/2014, 03:20

... 10 6/ml RAW 264.7 cells were treated with LPS (1 μg/ml) and AS1 01 (2 μg/ml) for h Formaldehyde (1% ) was added to the culture medium, and after incubation for 10 at 37°C, cells were lysed for 10 ... B in vitro Gene 19 94, 14 5 :19 7-203 doi :10 .11 86 /14 76-9255-7-3 Cite this article as: Brodsky et al.: The anti-inflammatory effects of the tellurium redox modulating compound, AS1 01, are associated ... cells (1 × 10 6/ml) were treated with AS1 01 for h (A) and h (C) Cytosolic and nuclear extracts were immunoblotted using anti-p65 of NFB and anti-actin Extracts were immunoblotted using RCC1 indicating...

Ngày tải lên: 11/08/2014, 08:22

... flavonoids and derivatives of chitin oligosaccharides Plant J 19 98, 14 :23-34 Page 10 of 11 (page number not for citation purposes) BMC Plant Biology 2007, 7: 21 10 11 12 13 14 15 16 17 18 19 20 21 22 ... in bacteria [17 ] A 17 73 bp-long DNA sequence spanning the coding region (16 71 bp) of the iaaM gene (GenBank accession n M 110 35) from Pseudomonas syringae pv savastanoi [ 31] and a 14 52 bp-long ... as internal standard Germinated seedlings of M truncatula were transferred in small pots and grown on a sand and perlite mixture (1: 1) in a growth chamber at 22°C and 10 -h light /14 -h dark regimen...

Ngày tải lên: 12/08/2014, 05:20

... ± 1. 9 10 .3 ± 1. 3 8.2 ± 1. 2 314 ± 37 4.4 ± 0.4 80 ± 23.9 ± 1. 7 10 .3 ± 1. 3 7.3 ± 1. 1 273 ± 32 NS NS 0.003 NS < 0.02 0. 015 ITBVI (ml/m2) PBVI (ml/m2) RVEDVI (ml/m2) 907 ± 48 19 6 ± 20 15 7 ± 11 915 ... 20.5 ± 1. 7 925 ± 66 16 5 ± 27 15 0 ± 10 21. 3 ± 2.3 NS NS NS NS PaO2/FiO2 (kPa) A–a gradient (kPa) Qs/Qt (%) 18 .0 ± 1. 1 41. 6 ± 3.8 33 .1 ± 2.0 18 .2 ± 1. 5 41. 8 ± 4 .1 34.7 ± 2 .1 NS NS NS NS, P > 0.2 ... 15 7 ± 11 915 ± 55 18 6 ± 27 14 9 ± 10 NS NS NS EVLWI (ml/kg) Total group Nonresponders Responders 18 .4 ± 1. 9 20.6 ± 3.5 16 .2 ± 1. 7 17 .1 ± 1. 6 19 .8 ± 3.0 14 .5 ± 0.9 0 .14 NS 0 .15 PaO2/FiO2 (kPa)...

Ngày tải lên: 12/08/2014, 18:21

... ± 5 .1% and 43.2 ± 4.3% for the control and for ET1, respectively) and for Bad (10 .1 ± 3.8% and 9.5 ± 2 .1% , respectively) Discussion This study shows an overproduction of NO, MMP -1 and MMP -13 in ... LY83583 0 .1 KT5720 CONTROL ET -1 (nM) Wortmannin 0.0 # * 10 0 SB20 219 0 0.5 200 PD98059 NO (% of control) ### # NO (nmol/5 10 5 cells) 1. 0 300 (b) ### 1. 5 (a) Effect of endothelin- 1 (ET -1) on nitric oxide ... significant Results ET -1 induces MMP -1 and MMP -13 production The effects of ET -1 and those of various inhibitors on MMP1 production and MMP -13 production are shown in Fig At 10 nM ET -1 the production...

Ngày tải lên: 09/08/2014, 06:22

... Cell Immunol 19 92, 14 1 :11 1 -12 0 Corradin SB, Mauel J, Donini SD, Quattrocchi E, Ricciardi-Castagnoli P: Inducible nitric oxide synthase activity of cloned murine microglial cells Glia 19 93, 7:255-262 ... (H2DCF-DA) 10 11 dichlorofluorescein (DCF) 2-amino-dihydro-6-methyl-4H -1, 3-thiazine (AMT) phenoxybenzamine (PB) 12 13 forskolin (FSK) phosphodiesterase (PDE) 14 Competing interests 15 None declared 16 ... expression J Neurochem 19 97, 69 :14 38 -14 48 Galea E, Feinstein DL: Regulation of the expression of the inflammatory nitric oxide synthase (NOS2) by cyclic AMP FASEB J 19 99, 13 : 212 5- 213 7 Zarow C, Lyness...

Ngày tải lên: 19/06/2014, 22:20

... physiological, and pathological perspectives, problems, and promises Dev Cell 2 011 , 21: 193- 215 23 Krueger M, Bechmann I: CNS pericytes: concepts, misconceptions, and a way out Glia 2 010 , 58 :1- 10 24 Saura ... non-amyloid angiopathies: FAD-PS -1 (P 117 L) mutation and CADASIL Immunohistochemical and ultrastructural studies Folia Neuropathol 2007, 45 :19 2-204 doi :10 .11 86 /17 42-2094-8 -13 9 Cite this article as: Kovac ... authors declare that they have no competing interests Received: 17 August 2 011 Accepted: 13 October 2 011 Published: 13 October 2 011 References Neuwelt E, Abbott NJ, Abrey L, Banks WA, Blakley B,...

Ngày tải lên: 19/06/2014, 22:20

... 0.05, **P < 0. 01 and ***P < 0.0 01 for comparisons between time zero with IL -1 ; +P < 0.05, ++P < 0. 01 and +++P < 0.0 01 for comparisons between untreated with IL -1 or IL -1 with IL -1 plus SB203580 ... constructs Biochem Biophys Res Commun 20 01, 285 :11 68 -11 74 17 Chowdhury TT, Bader DL, Lee DA: Dynamic compression counteracts IL -1 induced release of nitric oxide and PGE2 by superficial zone chondrocytes ... Am 19 99, 25:269-282 Evans CH, Watkins SC, Stefanovic Racic M: Nitric oxide and cartilage metabolism Methods Enzymol 19 96, 269:7 -13 Scher JU, Pillinger MH, Abramson SB: Nitric oxide synthases and...

Ngày tải lên: 09/08/2014, 10:23

... authors read and approved the final manuscript Acknowledgements The work in this study was supported by NIH grant NIDDK RO1-DK 06 613 8 and NIDRR grant H133E070024 References 10 11 12 13 14 15 Competing ... 2009, 73:4 21- 426 Linares-Fernandez BE, Alfieri AB: Cyclophosphamide induced cystitis: role of nitric oxide synthase, cyclooxygenase -1 and 2, and NK (1) receptors J Urol 2007, 17 7 :15 31- 1536 Hamby ... 33:S478-4 81 Page 11 of 13 (page number not for citation purposes) Journal of Inflammation 2009, 6:23 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 Vodovotz Y: Control of nitric oxide...

Ngày tải lên: 11/08/2014, 08:22

... 0 .11 2.50 ± 0 .11 b 9.55 ± 0.28 No-tr c 3D6d b 1. 40 ± 0 .11 a, b 2 .15 ± 0 .11 P 0.05 2.50 ± 0 .11 P > 0.05 2.70 ± 0 .11 P 0.05 2.20...

Ngày tải lên: 12/08/2014, 17:21

... conservative fluids, NO donor and NOS inhibitors Role of nitric oxide (NO) and PGE2 in prolonged hemorrhagic shocked rats and therapeutic effects of conservative fluids, NOS and COX-2 inhibitors Role ... 24, 48 and 72 hours in different 60 groups of rats Figure 4.4 .1 Hemotoxylin and eosin stain of Kidneys 62 Figure 4.4.2 Hemotoxylin and eosin stain of Lungs 63 Figure 4.4.3 Hemotoxylin and eosin ... sham-operated rats Figure 2.6 Hemotoxylin and eosin stain of kidney, liver, lung and 21 stomach of sham-operated rats and saline-treated rats Figure 3 .1 Survival percentages in different groups...

Ngày tải lên: 16/09/2015, 15:54

... no conflict of interest exists References 10 11 12 13 14 15 Coyle JT, Puttfarcken P Oxidative stress, glutamate and neurodegenerative disorders Science 19 93; 262: 689-695 Platt SR The role of glutamate ... Neurosci 19 94; 17 : 359-365 Van der berg CJ, Garfinkel D A stimulation study of brain compartments, metabolism of glutamate and related substances in mouse brain Biochem J 19 71; 12 3: 211 - 218 Berman ... 2007; 14 5: 256-264 http://www.medsci.org Int J Med Sci 2 010 , 16 Wiesinger H Arginine metabolism and the synthesis of nitric oxide in the nervous system Prog Neurobiol.20 01; 64: 365-3 91 17 Prast,...

Ngày tải lên: 26/10/2012, 09:07

... [58,59, 61, 90,99] [ 31 33 ,10 2 ,10 5] [28 ,10 2 ,10 5 ,11 5] [34,35] [77] [ 61, 72 ,11 6] [60] [88] [11 7] [47,48] [50 ,11 8 12 2] [49 ,12 3] [12 4 ,12 5] e a Unless otherwise stated, cytochrome c reduction and NO synthesis ... Biol Chem 275, 612 3– 612 8 11 8 Bender AT, Silverstein AM, Demady DR, Kanelakis KC, Noguchi S, Pratt WB & Osawa Y (19 99) Neuro- 3974 11 9 12 0 12 1 12 2 12 3 12 4 12 5 12 6 12 7 12 8 nal nitric- oxide synthase ... 99 10 0 10 1 10 2 10 3 10 4 10 5 ment by laser flash photolysis of intramolecular electron transfer in a two-domain construct of murine inducible nitric oxide synthase J Am Chem Soc 12 8, 3808–3 811 Feng...

Ngày tải lên: 18/02/2014, 11:20

... treated with LPS/IFN and VSMC treated with TS and LPS/IFN (B) KT: KT-5720, Ro: Ro 31- 8220, GF: GF109203X The concentrations of KT, H8, Ro, GF and TS were 1, 20, 5, 10 and 10 lM, respectively Values ... alone As shown in Fig 4, addition of LPS alone (10 mgÆmL )1) or IFN alone (10 0 UÆmL )1) induced the NO productions but were very feeble as 1. 1-fold and 1. 4-fold of control, respectively These results ... for their potential antineoplastic role Redox Report 6, 14 3 15 1 17 Jeremy, J.Y., Rowe, D., Emsley, A.M & Newby, A.C (19 99) Nitric oxide and the proliferation of vascular smooth muscle cells Cardiovasc...

Ngày tải lên: 22/02/2014, 04:20

... 80 10 0 12 0 14 0 16 0 18 0 60 80 10 0 12 0 14 0 16 0 18 0 SO2 = 65% 14 0 12 0 12 0 10 0 10 0 80 80 60 60 40 40 20 20 0 20 40 60 80 10 0 12 0 14 0 F 16 0 SO2 = 78% 14 0 16 0 18 0 20 G 16 0 40 16 0 14 0 14 0 SO2 = 10 0% ... 2.7 12 0 10 0 10 0 80 80 60 60 40 40 20 20 0 C SO2 = 32% [ATP]/[Hb] = 14 0 20 40 60 80 10 0 12 0 14 0 16 0 18 0 D 16 0 SO2 = 70% [ATP]/[Hb] = 20 40 60 80 10 0 12 0 16 0 18 0 16 0 18 0 16 0 14 0 SO2 = 10 0% 12 0 12 0 ... 64% 78% 60 80 10 0 12 0 14 0 16 0 18 0 14 oxyHb reaction rate 12 10 40 [ATP]/[Hb] = 10 SO2 10 0% 32% 70% 4 2 0 20 40 60 80 10 0 12 0 14 0 16 0 18 0 Time (min) 20 40 60 80 10 0 12 0 14 0 16 0 18 0 Time (min)...

Ngày tải lên: 16/03/2014, 06:20

... A5 21 V522 L523 F524 A525 C526 M527 L528 Glu 11, Glu14, Ala15, Leu18 C-terminal domain Central helix Val 91, Phe 91, Leu 112 Ser 81, Glu84, Ile85, Ala88, Met145 Glu 11, Phe12, Ala15, Met72 Ala15, Leu18, ... (EDTA) 10 0 93 10 0 ± 37 ± NAA 90 ± NAA NAA 10 0 ± NAA NAA 81 ± NAA NAA 10 0 ± 5± NAA 11 5 ± 4± NAA 10 0 ± 17 ± NAA 11 1 ± 43 ± NAA 10 0 ± 5± NAA 98 ± 17 ± NAA ± ± ± ± ± ± 2 FEBS Journal 273 (2006) 17 59 17 71 ... 2 71, 14 6 31 14 635 10 Stevens-Truss R, Beckingham K & Marletta MA (19 97) Calcium binding sites of calmodulin and electron transfer by neuronal nitric oxide synthase Biochemistry 36, 12 337 12 345 11 ...

Ngày tải lên: 16/03/2014, 13:20