emission of pure solid films and pure liquid films

... Configuration 36 3.2.2 Design of Emission Cell 37 3.2.3 Pure Solid Film and Pure Liquid Film Samples 39 3.2.4 Liquid- Phase Organic Reaction System 40 3.3 Emission Model 42 3.3.1 Emission Spectral Presentation ... Discussion: Emission of Pure Solid Films and Pure Liquid Films 51 3.5.1 Pure Solid Films: Parafilm 52 3.5.1.1 Experimental Data Sets 52 3.5.1.2 Signal Processing and SVD 55 3.5.1.3 Spectral Reconstruction ... 3.4 Emission Mode Band-Target Entropy Minimization 47 3.4.1 Formulation of Emission BTEM 47 iv 3.4.2 Quantitative Analysis: Concentration Profiles 51 3.5 Results and Discussion: Emission of Pure...

Ngày tải lên: 12/09/2015, 08:20

... // Handle where there is more than one record with the nth value // Eliminate enough rows from the bottom of the dv using a filter on // the primary key to return the correct number (top n) of ... more than one instance of the value in the nth record, as is the case with Freight In this case, iterate over the records following the nth record and add criteria to a copy of the data view filter ... field in descending order; this places the top n records at the top of the view Next, get the Freight value for the nth record and set the DataView filter to contain only rows with a Freight value...

Ngày tải lên: 14/12/2013, 18:16

... of England All of these forecasts, and literally millions of others of a similar degree of certainty, are based on the specific understanding of the values and goals motivating millions of nameless ... reaction Understanding deals with judgments of value, with the choice of ends and of the means resorted to for the attainment of these ends, and with the valuation of the outcome of actions performed.21 ... the starting point of a specific mode of reflection, of the specific understanding of the historical sciences of human action.” Equipped with the method of “specific understanding,” the historian,...

Ngày tải lên: 17/01/2014, 02:20

... N Problem 2-17 Determine the magnitude and direction of the resultant force F R Express the result in terms of the magnitudes of the components F and F and the angle φ Solution: F R = F + F2 ... directed along the keel aa as shown, determine the magnitudes of forces T and P acting in each rope and the angle θ of P so that the magnitude of P is a minimum T acts at θ from the keel as shown Given: ... along the positive y axis, determine the magnitudes of forces FA and F B acting on each chain and the orientation θ of F B so that the magnitude of FB is a minimum Given: F = 600 N θ = 30 deg Solution:...

Ngày tải lên: 17/02/2014, 14:20

... and 3¢ HindIII antisense primers were used to amplify the su9-DHFR and cloned into the same sites of yeast vector pNS61 was cut with Spe1 and Xho1 and cloned into same sites of yeast vector of ... determinations of the supernatant containing resorufin were made at the excitation and emission wavelengths of 528 nm and 590 nm, respectively The ERND activities of mitochondria and mitoplast ... completely degraded TIM23 and FDXR Figure 3D represents a full view of an immunoblot of mitochondrial and microsomal proteins developed with a combination of CYP2E1 and TIM23 antibodies It is...

Ngày tải lên: 18/02/2014, 16:20

... stefin A and stefin B at pH and variant of stefin B and the P79S mutant of the variant at both pH and pH Each panel is a plot of heat change on ligand addition (kcalÆmole)1) against the ligand ⁄ stefin ... and B and the P79S mutant The two isoforms of stefin B have exactly the same far UV CD Regardless of the sequence differences (as highlighted in Fig 1), the secondary and tertiary structures of ... Fig Circular dichrosim spectra of stefin B and of stefin A (A) Far UV CD spectra of stefin A, stefin B and the P79S mutant (B) Near UV CD spectra of stefin A, stefin B and the P79S mutant that the protein...

Ngày tải lên: 19/02/2014, 06:20

... 4-aminobutyrate and CH3NH2 as carbon source (A) Seven microliters of medium of a 10 mL culture grown for h (lanes and 6), for h (lanes and 7), for h (lanes and 8), and for h (lanes and 9) on minimal ... study and RT-PCR analysis of transcripts (A) Schematic representation of the pAO1 gene and ORF cluster flanked by Tn554 and DTn The cluster consists of the pmfR gene, encoding the regulator of the ... bioremediation of nicotine waste, but also in the supply of nicotine derivatives as starting materials for the synthesis of new products of industrial and pharmaceutical importance [3,4] Construction of...

Ngày tải lên: 19/02/2014, 07:20

... group of C115 is highlighted in green and the nitrogen atoms of R120 and K22 in blue The open form of MurA is unliganded (A) and the closed conformation (B) contains fosfomycin (orange) and UDPNAG ... independent of the thermodynamic and kinetic properties of the binding process and is solely based on the detectability of the peptides of interest We have used this method to show that binding of fosfomycin ... charge of the amino acid side chain occupying this position Taken together, our combined calorimetric and protein chemical approach leads to a more detailed understanding of the role of K22 and...

Ngày tải lên: 19/02/2014, 13:20

... half-lives of 22 and 23 h for wild type GAT1 in the absence and presence of dMM, respectively, and of 12 and 5.5 h for the DDN and DDQ mutants, respectively GABA values of NNN treated with dMM, and of ... activities of the N-glycosylation 1628 Fig Determination of expression of GFP-tagged GAT1 mutants on the surface of transfected CHO cells and measurement of GABA-uptake by GFP-tagged GAT1 wild type and ... with N-glycans of mature complex type and the small band to the proteins with N-glycans of mannose-rich type All bands of the mutants had reduced molecular mass, compared with that of wild type...

Ngày tải lên: 19/02/2014, 17:20

... bands are stained, with two intense signals of 70 and 50 kDa, while the bands at 60 and 150 kDa vanished With oxyblot (Fig 4C), antibodies stained carbonylated proteins mainly in band ranges of ... comparable pattern of bands, although two bands of 60 and 150 kDa strongly visible at 10 months (lane 1) were absent at 27 months, and an important band of 70 kDa (lane 2) emerged in this latter ... consumption in state and in state 4, respiratory control ratio (RCR) of oxygen consumptions in states and and the ADP/O ratio were calculated Oxygen consumption in the presence of 40 lM of dinitrophenol...

Ngày tải lên: 20/02/2014, 11:20

... constructed a number of single (R86A, R90A and R131A) and double (H94A ⁄ R100A and R90A ⁄ R131A) point mutations in the N-domain of ClpA (Fig 2A) First, we compared the degradation of SsrA-tagged GFP ... of this substrate class requires specific interaction between ClpS and the N-domain of ClpA Consequently, dramatic changes to the structure of the N-domain of ClpA would inhibit ClpS binding and ... presence of RR ⁄ AA and ATP (D) The relative amount of ‘unfolded’ GFP–ssrA (29 130 Da) was determined in the presence of wild-type ClpA (open circles) or RR ⁄ AA (filled circles) presence of ClpA and...

Ngày tải lên: 07/03/2014, 05:20

... expressed as the percentage of the maximal value obtained at the end of the assay by the addition of mM Triton X-100 The excitation and emission wavelengths were set to 485 and 520 nm Both slits were ... oligomers of vesicles made of acidic phospholipids resembles pore formation by A-b [27] and liposome permeabilization of a-synuclein [43] The toxic activity exerted by prefibrillar forms of stefin B and ... transferred to another tube and the released calcein measured using a Jasco FP-750 spectrofluorimeter (Jasco, Easton, MD), with excitation and emission at 485 and 520 nm Excitation and emission slits were...

Ngày tải lên: 07/03/2014, 17:20

... effect of systematic substitution of Arg46 on intracellular signalling The stimulation of accumulated InsPs by increasing concentrations of AVP, was assayed for each of the 19 mutant constructs and ... activation of the V1aR Discussion Receptors which mediate the effects of the neurohypophysial peptide hormone family are structurally and pharma- Fig Effect of substitution of Arg46 of the V1aR ... aromatic ring of phenylalanine had a neutral effect as the sensitivity of [R46F]V1aR and [R46Y]V1aR to AVP was almost identical The residues Trp and Gly represent the two extremes of side-chain...

Ngày tải lên: 07/03/2014, 21:20

... superimposition of TAP1 and HisP, which does contain a bound ATP molecule [30] Analysis of the individual P-glycoprotein NBD models in terms of stereochemistry and local packing enabled the selection of a ... to a concentration of mgÆmL)1 and incubated with pre-equilibrated amylose resin (New England Biolabs) at a protein/resin ratio of : (w/v) for 30 at room temperature Resin and protein were loaded ... mean ± SEM Molecular modelling of P-glycoprotein NBDs Sequences of P-glycoprotein NBDs and TAP1 were aligned using CLUSTALW [34] A series of 10 homology models of each NBD was constructed, using...

Ngày tải lên: 08/03/2014, 08:20

... nitrobluetetrazolium and 5-bromo-4-chloro-3-indolylphosphate The protein bands on the immunoblots was quantified by densitometry scanning and the intensity of each band was determined using the NIH Image software ... are located in (Cys212 and Cys214), and around (Cys106 and Cys235) the GT1 motif Mutation of C106A, C212A, and C214A, resulted in a considerable decrease in secretion of the mutant proteins (Fig ... relative activities of C212A and C214A were drastically decreased, to 6% and 17% of that of P-DN42, respectively These results indicate that the conserved Cys106, Cys212, and Cys214 residues...

Ngày tải lên: 08/03/2014, 10:20

... model of Kv3.1 and amino acid sequences of the S1–S2 linker of Kvs (A) Topology of a Kv3.1 monomeric unit Black circles represent the Asn of native N-glycosylation consensus sites N220 and N229 ... utilized in rat brain and infected Sf9 cells, and the role that occupancy of these sites has in the expression of functional Kv3.1s at the cell surface of Sf9 cells Immunoband patterns of wildtype Kv3.1, ... electrophoretic migration pattern of wild-type Kv3.1 revealed a predominant immunoband at about 87 kDa and two lower faint bands The lowest band migrated to about 77 kDa, and the middle band was at about 81...

Ngày tải lên: 16/03/2014, 13:20

... 11 Ó FEBS 2004 Analysis of the N-myristoylation consensus motif (Eur J Biochem 271) 867 The levels of incorporation of [3H]Leu and [3H]myristic acid into the lower band of the expressed MGA(8)-TNF ... quantitative analysis of the fluorograms of [3H]myristic acid- and [3H]leucine-labeled proteins shown in the lower and upper panels of (A) Relative N-myristoylation efficiency of each MG6X-TNF was ... efficiency of N-myristoylation of various cellular proteins may arise in part because of differences in the presentation of Gly2 dictated by interactions among the residues at positions 3, 6, and of...

Ngày tải lên: 16/03/2014, 16:20

... role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of transcription Ó FEBS 2004 Regulation of the presenilin gene (Eur J Biochem ... Pastorcic and H K Das (Eur J Biochem 271) Ó FEBS 2004 Fig Deletion mapping of the PS1 promoter The positions of the 5¢- and 3¢-ends of each deletion fragment are indicated on the left (5¢D) and on ... Regulation of the presenilin gene (Eur J Biochem 271) 4489 A Fig Effect of PS1 promoter point mutations on the efficiency of transcription initiation and 23 the position of the start site(s) (A) Effect of...

Ngày tải lên: 16/03/2014, 18:20

... on the solution structure of monomeric derivatives, we analysed the 2D NMR spectra of the different variants of mBS Figure shows the expanded regions of TOCSY spectra of L28Q-mBS (panel L28Q), ... Professor Giuseppe D’Alessio for critical reading of the manuscript We also thank Professor Alberto Di Donato and Dr Valeria Cafaro for their kind hospitality and advice on protein expression and ... analysis of an analog of an intermediate formed in the ratedetermining step of one pathway in the oxidative folding of bovine pancreatic ribonuclease A: automated analysis of 1H, 13C, and 15N...

Ngày tải lên: 16/03/2014, 23:20

... W366 and Y394 residues of the D2 domain may play a role similar to that of binding sites I and II of GA-1 Mutations of W366 and Y394 in D123 decreased the starch-binding capacity by three- and ... with the pull-down and far western assays, showing a lack of interaction for the individual SBDs and also in the absence of the D(316–344) and D(495–535) regions of the D2 and D3 domains, respectively ... highlighted by black bars (b strand), grey bars (helix) and white bars (coil) D2 and D3 domains are indicated by arrows Stars indicate the positions of W366 and Y394 D(316–344) and D(495–535) regions...

Ngày tải lên: 22/03/2014, 21:20