emerging families of coat proteins
Use of Cyanobacterial Proteins to Engineer New Crops
Ngày tải lên :
25/10/2013, 05:20
... biofarming is a major goal of plant biotechnology Direct transfer of plant genes has resulted in new varieties with improved properties However, this approach is limited by the genetic stock of ... the various existing Use of Cyanobacterial Proteins 67 Fig 4.1 Summary of current advances in plant biotechnology using cyanobacterial genes Gene mining on cyanobacteria offers a promising opportunity ... phytochrome reaction spectra Production of biodegradable polymers (Section 4.5) Phytochrome perception (Section 4.6) (synthesis of PCB) Biofarming Production of cyanophycin Pigment manipulation (Section...
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Tài liệu Three Families of Lines docx
Ngày tải lên :
26/01/2014, 08:20
... 2 THREE FAMILIES OF LINES Lines visually separate and/or define the forms, shapes, and patterns of the various components of a drawing Straight, angle, and curved ... curriculum development This site offers downloadable and printable drawing classes for students of all abilities from the age of eight through adult Students of all ages, levels and abilities ... compound curves, such as in the letter “S”? Examine the sizes and shapes of the spaces on either side of each line Study the outlines of shapes, such as circles, ovals, rectangles, triangles, or squares...
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Tài liệu Báo cáo khoa học: Seeking the determinants of the elusive functions of Sco proteins pptx
Ngày tải lên :
14/02/2014, 18:20
... divergence of Sco proteins in prokaryotes is apparent from the analysis of their genomes, Determinants of the elusive functions of Sco proteins some of which contain genes coding for Sco proteins ... variety of possible functions Given that the fold of Sco proteins resembles that of proteins involved in thiol-disulfide redox chemistry, it is reasonable to assume that the primary role of Sco proteins ... oxidoreductase function of the CXXXC motif of human Sco proteins could therefore be implicated not only in the maturation of the CuA site of Cox2 but also in the maintenance of cellular copper homeostasis...
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Tài liệu Báo cáo khoa học: The social life of ribosomal proteins doc
Ngày tải lên :
19/02/2014, 17:20
... functional effects of these mutations were due to the architectural and stabilizing role of the proteins alone, i.e that perturbation of protein structure would affect the catalytic activity of the rRNA ... composed of RNA However, two long C-terminal tails of small ribosomal subunit proteins S9 and S13 that are otherwise located at the top of the 30S, make their way down through the head of the subunit ... antiterminator of transcription of k phage N protein in E coli [58] Furthermore, several ribosomal proteins are known to regulate the expression of themselves or other ribosomal proteins through...
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Tài liệu Báo cáo khoa học: Physiological relevance of the endogenous mono(ADP-ribosyl)ation of cellular proteins doc
Ngày tải lên :
20/02/2014, 02:21
... Overall, a better understanding of the various and diverse biological roles of ADP-ribosylation of cellular proteins and peptides will be essential to fully define the role of this modification in normal ... domains of the ARTs are involved in the regulation of ART activity has been recently investigated by measuring both the transferase and NAD glycohydrolase (NADase) activities of truncated mutants of ... inhibited in the presence of NAD via the ADP-ribosylation of integrin LFA-1 and other coreceptor proteins [37,38] These effects have been proposed to result from a failure of the T-cell receptors...
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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx
Ngày tải lên :
20/02/2014, 23:20
... presence of turnover of the protein Quantitation of the Mcm4 band suggested that the protein stability of Mcm4 does not greatly differ between HeLa and WI-38 cells (Fig 5C) Expression of Mcm3 and proteins ... level of 33–38 were used for experiments and had almost stopped proliferating at approximately 41 population doubling level Fractionation of cell extracts and Western-blot analysis of Mcm proteins ... precipitate was suspended in 0.1 mL of solution A and then mixed with 0.05 mL of concentrated SDS sample buffer Total cellular proteins and chromatin-bound proteins were electrophoresed on a 10%...
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Tài liệu Committee on Foreign and Emerging Diseases of the United States Animal Health Association pptx
Ngày tải lên :
22/02/2014, 05:20
... Bureau of Indian Affairs Support roles are also played by the Department of Transportation, the Department of Defense, the Department of Commerce, the Department of Justice, the Department of the ... detection of virulent strains, identification of genetic sources of antimicrobic resistance, location of virulence factors, recognition of vaccine escape mutants, epidemiologic investigation of disease ... agent of interest The obvious strength of histochemical and in situ nucleic acid detection of agents is that detection and localization of the agent in question is interpreted in context of the...
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THE RAPID EVOLUTION OF REPRODUCTIVE PROTEINS pptx
Ngày tải lên :
05/03/2014, 17:20
... other species The study of the mechanisms of speciation is one of the central areas of interest in evolutionary biology Although the role of rapid evolution of reproductive proteins in the speciation ... show the generality of the phenomenon of rapidly evolving reproductive proteins But what is the theoretical outcome of the continual coevolution of pairs of gamete-recognition proteins? Computer ... sequences of lysins from different abalone species are remarkably divergent and are excellent examples of adaptive evolution25,26 The cause of rapid evolution of lysin might lie in the structure of...
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Báo cáo khoa học: Functional classification of scaffold proteins and related molecules pptx
Ngày tải lên :
06/03/2014, 22:21
... classification of scaffold proteins L Buday and P Tompa Table Scaffold proteins and their kin Representatives of the four categories of scaffold proteins and their relatives are presented The length of the ... functional Scaffold proteins may be considered as the founders of this functional class, epitomizing the very essence of the action of signaling regulatory proteins [5] They are defined as proteins organizing ... regulatory proteins It will be addressed that scaffold proteins and their kin not only bring signaling proteins together, but also regulate the flow of signaling information using a variety of mechanisms,...
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Báo cáo khoa học: Synthesis and function of ribosomal proteins – fading models and new perspectives pptx
Ngày tải lên :
07/03/2014, 01:20
... activity of RP mRNA for adjustment of the biosynthesis of ribosomes to the requirements of cell growth and differentiation In addition, a relevant contribution of protein turnover to the regulation of ... of an RP in mice was an inducible deletion of both copies of the RPS6 gene in the liver of adult mice [77] In this study, the altered response to partial hepatectomy suggested the existence of ... the activation of a p53dependent ‘ribosomal checkpoint’ This is the first response of the cell to a ribosome defect, and consists of blocking of the cell cycle and ⁄ or activation of apoptosis mediated...
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Báo cáo Y học: The presence of a helix breaker in the hydrophobic core of signal sequences of secretory proteins prevents recognition by the signal-recognition particle in Escherichia coli doc
Ngày tải lên :
08/03/2014, 09:20
... SecB dependency of the targeting of mutant prePhoE By the substitution of an a-helix-promoting residue (Leu, Ala or Cys) for the helix-breaking Gly)10 of the signal sequence of PhoE, the a-helix ... dependency of the precursor on SecA and SecY, whereas its SecB dependency is reduced Affinity of mutant prePhoE nascent chains for P48 As the SecB dependency of the translocation of the mutant prePhoE proteins ... overloading of the SRP pathway The re-routing of (G-10L)prePhoE to the Sec translocon via the SRP instead of the SecB pathway could be explained by the increased hydrophobicity of the hydrophobic core of...
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Sri lanka the emerging wonder of asia mahinda Chintana Vision for the future pot
Ngày tải lên :
08/03/2014, 23:20
... of aquaculture centres No of ornamental fish breeding and training centres (Nos) No of new cage farms No of fish processing zones Share of storage capacity Certification and standardization of ... capacity No of calves produced/ yr No of farms No of farms No of calves/heifers/yr Development of the NLDB farms on the basis of public-private partnership No of farms developed /yr Providing support ... aspects of rural centric development initiatives and ensure effective coordination of service delivery of a wide range of Government activities at the village level The Introduction of Pro-Regional,...
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Báo cáo khoa học: The association of viral proteins with host cell dynein components during virus infection pdf
Ngày tải lên :
14/03/2014, 22:20
... rhabdovirus is a cofactor of the RNA polymerase complex and, in fact, facilitates the binding of the polymerase to the N-RNA complex [67] Using the SAD-D29 low-virulent strain of RV, deletion of the DYNLL1 ... KSTQT and KSIQI in the case of the P protein of rabies and Mokola viruses respectively [70,71] and SQTQT in the case of protein VP35 of ebolavirus [103] The presence of these sequences that closely ... in agreement with the modeled solution structure of the complex of DYNLL1 with p54 of ASFV [26] (B) Three hypotheses for the association of viral proteins to the dynein molecular motor One viral...
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Báo cáo Y học: Conformational analysis of opacity proteins from Neisseria meningitidis ppt
Ngày tải lên :
17/03/2014, 10:20
... Conformation of meningococcal Opa proteins (Eur J Biochem 269) 5219 Fig Semi-native PAGE analysis of in vitro folding of OpaB128 (A) and OpaJ129 (B) (A) Semi-native-PAGE analysis of in vitro folding of ... GmbH, Freiburg, Germany) for the detection of the N-terminal domains of the CEACAM proteins Production and purification of inclusion bodies Cultures of the E coli strain BL21 (DE3) containing ... fragment of opaB128 and opaJ129 encoding the mature domain of the protein downstream of the promoter and the signal sequence-encoding part of the phosphate-limitation inducible phoE gene of E coli...
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Báo cáo khoa học: Molecular characterization of secretory proteins Rv3619c and Rv3620c fromMycobacterium tuberculosisH37Rv docx
Ngày tải lên :
22/03/2014, 16:21
... measurements of the interaction between Rv3619c and Rv3620c in phosphate buffer at 25 °C; raw data of heat effect (lcalÆs)1) of 30 injections (10 lL each) of 0.1 mM Rv3619c into 1.43 mL of 0.01 mM ... square fit The heat of dilution was subtracted from the raw data of titration of Rv3619c with Rv3620c (B) Far-UV CD spectra of Rv3619c, Rv3620c, and the : complex CD spectra of lM Rv3619c (j), ... ESAT-6, our study supports the hypothesis of Simeone et al [26] that the biological activity of ESAT-6 depends on the pH of phagosomal compartments Proteins of the ESAT-6 family are not only involved...
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Báo cáo khoa học: Natural and amyloid self-assembly of S100 proteins: structural basis of functional diversity doc
Ngày tải lên :
23/03/2014, 03:20
... the promiscuity of S100 proteins Therefore, systematic studies of the conformational changes and oligomerization of S100 proteins will be of critical importance in the development of potential therapeutics ... function of S100 proteins are modulated by metal ion binding The binding of Ca2+ to EF-hand type domains triggers conformational changes allowing interactions with other proteins In many S100 proteins, ... how the binding of different metal ions to S100 proteins has the potential to result in conformational adjustments and modulation of protein folding and functions A number of S100 proteins also...
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Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
Ngày tải lên :
23/03/2014, 13:20
... graphs represent the percentage of the binding activity of a shifted complex (the ratio of the density area in arbitrary scanning units, and the sum of the density areas of all shifted complexes, including ... were done in the presence of a standard concentration of KCl (100 mM) used in normal reactions and varying concentrations of MgCl2 (0–50 mM), or of a standard concentration of MgCl2 (5 mM) used in ... fractions of S100 and nuclear extracts from L amazonensis promastigotes Due to the limiting amount of telomeric proteins present in these extracts, binding reactions were done with a minimum of lg of...
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Báo cáo khoa học: Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7 pot
Ngày tải lên :
23/03/2014, 13:20
... cleavage of essential yeast proteins To assess the activity of caspase-2 in yeast, various constructs encoding different forms of the protein (Fig 1) were transformed into yeast (Fig 2A) Expression of ... efficient separation of large and small subunits of the D330A mutant In contrast, mutation of the murine equivalent of the human residue D316 (D333) alone prevented autoprocessing of caspase-2 [17,38] ... least in the absence of an activating adaptor, generation of active human caspase-2 requires separation of its large and small subunits In the context of autoactivation, removal of the prodomain...
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Báo cáo khoa học: F1 – Structure and Function of Motor Proteins pot
Ngày tải lên :
23/03/2014, 15:20
... Laboratory of Biophysical Chemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran E-mail: divsalar@ibb.ut.ac.ir One of the most widely studied and fascinating proteins of ... Group, Department of Biophysics, University of Pe´cs, Pe´cs, Hungary, Protein Research Group of Hungarian Academy of Sciences, Department of Medicinal Chemistry, University of Szeged, Szeged, ... kinds of parts Control of the direction of rotation of these motors is the basis for the chemotactic response, i.e for the ability of cells, such as E coli, to swim up spatial gradients of chemical...
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Báo cáo khoa học: The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system ppt
Ngày tải lên :
29/03/2014, 21:20
... efficiency of protein N-myristoylation and the identity of the amino acids at positions 3, and of the consensus motif in plant target proteins with the use of a Cell-free N-myristoylation of plant proteins ... N-myristoylation of two proteins of A thaliana as model substrates: RING domain ligase (RGLG2) and ADP-ribosylation factor (ARF1A1c) N-myristoylation of these proteins was described previously, and that of ... Analysis of in vitro-synthesized N-myristoylated proteins To confirm the N-myristoylation of proteins synthesized with the cell-free system, we next performed MALDI-TOF MS analysis Wild-type forms of...
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