embryonic cannabinoid receptors are targets for natural and endocannabinoids during early pregnancy

Báo cáo khoa học: " relationships for carbon and nitrogen during early growth of Juglans regia L. seedlings: analysis at two elevated CO concentrations 2" doc

Ngày tải lên : 08/08/2014, 14:21

... in C assimilation alter 1) the import of maternal C and N, 2) N uptake and assimilation, 3) partitioning of C and N between shoot and roots and 4) the time lapse prior to a complete independence ... available during the early stages of seedling growth were investigated by using the natural differences in the abundance of the staN N ble isotopes 15 and 14 in the nutrient solution and the seed ... weighed and ground to a fine , homogeneous powder with a laboratory mill Samples were stored at -20 °C before analysis of biochemical content and isotope composition Total C and N contents and isotope...

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Báo cáo khoa học: K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase pdf

Ngày tải lên : 23/03/2014, 15:20

... K9 and K18 Discussion This study provides evidence (a) that K4, K9 and K18 in histone H3 are good targets for biotinylation by human biotinidase; (b) that K14 and K23 are relatively poor targets ... associated with gene silencing [12] Third, K4 and K9 are targets for both methylation [2] and biotinylation; methylation and A B Fig Biotinylated histones H3 are present in extracts from human cell ... Zealand rabbits, following NIH and USDA guidelines for animal care All possible measures were taken to minimize pain and discomfort to animals Booster injections were given after 14, 21 and 49...

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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

Ngày tải lên : 07/03/2014, 04:20

... extension remains unstructured and solvent exposed, and therefore able to perform its solubilizing role in the sHsp and in the complexes formed with target proteins during chaperone action Indeed, ... °C for E190A and 70 °C for E199A and E204A Decreased light scattering after maximum precipitation had been reached resulted from the precipitate sinking to the bottom of the cuvette and therefore ... property that has also been observed recently for wildtype and mutant forms of aB-crystallin [60,61] The E190A and E204A mutants performed poorly compared with the wild-type protein in both assays,...

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Parasite Management for Natural and Organic Poultry: Coccidiosis pptx

Ngày tải lên : 23/03/2014, 21:20

... advantages and disadvantages for coccidial control Extreme heat and cold outdoors can reduce sporulation or kill oocysts Yet warmth and moisture are favorable conditions for coccidia Before the ... drugs are effective only in the ﬁrst part of the parasite life cycle, and therefore must be used early if they are to be used at all Drawbacks of using preventative drugs are their expense and ... decomposition of litter and manure and by the action of molds and bacteria However, there are usually so many oocysts that birds continue to pick them up and get sick Symptoms and Diagnosis Outward...

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Báo cáo y học: " How Many Steps/Day are Enough? for Children and Adolescents" docx

Ngày tải lên : 14/08/2014, 08:20

... reported normative data for a U S sample in 2002 and at that time suggested that the mean values of 13,000 for U.S boys and 11,000 for U.S girls could be used as reasonable standards for evaluation purposes ... activity accounts for ≅47-56% and ≅4759% (boys and girls respectively) of daily steps on school days [11] Differences among countries are apparent, with children from North America (Canada and United ... pedometers are more likely to be adopted for clinical and public health applications, and ultimately are also more likely to be embraced by the public themselves, due primarily to interpretability and...

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Báo cáo khoa học: The male seahorse synthesizes and secretes a novel C-type lectin into the brood pouch during early pregnancy pdf

Ngày tải lên : 07/03/2014, 17:20

... the embryos during early pregnancy CTLs are found universally in eukaryotes and prokaryotes and have diverse functions [10] Although often containing several domains, they are characterized by ... ligase at °C for days and then packaged into Gigapack III Gold Packaging Extract (Stratagene) by incubating at room temperature for h, before addition of SM buffer (500 lL) and chloroform (20 lL) ... incubated at 37 °C for 15 min, 20 mL of LB broth was added and the mixture incubated for 2.5 h at 37 °C with shaking The mixture was heated at 65 °C for 20 min, spun (1000 g for 10 min) and lL of the...

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Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx

Ngày tải lên : 14/02/2014, 22:20

... integrin receptors and promotes monocyte chemotaxis through a4 and b2 integrin receptors [15] Angiopoietin signaling is regulated by two independent receptors; Tie2 receptor and integrins [22] Therefore, ... focus on the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, and discuss whether these ANGPTLs could be targets for the prevention and treatment of these conditions ... ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases The expression of ANGPTL2 and ANGPTL6 ⁄ AGF is induced in obese conditions ANGPTL2 induces chronic adipose tissue inﬂammation and...

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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

Ngày tải lên : 19/02/2014, 16:20

... receptor gene expression tion and cells lysed and prepared for luciferase assays as described above HEK293 and HEL cell supernatants were assayed for both ﬁreﬂy and renilla luciferase activity ... markers (kDa) are indicated to the left and right of the (C) and (D), respectively, whilst the position of the Oct-1 (98 kDa approximately) and the two major forms of Oct-2 (75–80 and 55–60 kDa) ... autoradiography, as outlined (C and D) Western blot analysis of Oct-1 (C) and Oct-2 (D) expression in whole cell protein (60 lgÆ lane)1) prepared from HEL (C and D; lane 1) and HEK293 (C and D; lane 2) cells...

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Tài liệu Báo cáo khoa học: "Combining Functionality and Object Orientedness for Natural Language Processing" ppt

Ngày tải lên : 21/02/2014, 20:20

... There is no other means for manipulating data Objects can be classified into classes and instances A class defines a procedure [called a method) for handling incoming messages of its ... (are functions of, to be more precise) verb phrases But unlike Montague grammar we not use iambda expressions to bind case elements Instead we use special functors standing for case markers For ... (*subject(he)X(*object(it)Xeats)) A class is defined for each constant of PAL A class object for a lexical item contains linguistic knowledge in a procedural form In other words, a class contains information as to how a corresponding...

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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

Ngày tải lên : 07/03/2014, 15:20

... cells The expression and carboxypeptidase activities of the individual constructs were analyzed both in cell lysates and conditioned media, and the results are summarized in Fig and Table 2, respectively ... fold are cycled via the calnexin-calreticulinglucosidase I and II system within the ER lumen, providing space and time for the unfolded/partially folded proteins to acquire the correct 3D conformation ... enzymatic properties and structure/folding The results clearly show that the amino acids at the extreme C-terminus of GCPII are crucial for the hydrolytic activity of the enzyme and, furthermore,...

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Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt

Ngày tải lên : 07/03/2014, 16:20

... ethanolamine and mm choline, and metabolic labeling was performed with 20 lCi [14C]ethanolamine for 23 h at 25 °C For radiolabeling of double mutant strains, cells were grown in SGlyYE medium for days ... grown and [3H]inositol-labeled in glucose-containing medium Lipids were extracted from cells and incubated either with or without mild-base (lanes and 2) and with or without PI-PLC (lanes and 4) ... pathway, and further, Ybr004cp-depletion gives rise to cell wall and morphological defects characteristic of GPI assembly mutants We therefore propose that Ybr004cp is an excellent candidate for GPI-MT-II...

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Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt

Ngày tải lên : 08/03/2014, 02:20

... Im-Py targets GÆC base pairs and Py-Im targets CÆG base pairs and the Py-b-alanine pair recognizes both AÆT and TÆA base pairs [38] The boxed sequence represents the atypical R2 TATA-box Black and ... composed of two subunits, proteins R1 and R2 Both subunits are required for enzyme activity and it could be reasonable to assume that the expression of the R1 and R2 mRNAs should be coordinated ... either contain the natural R2 TATA-box or a mutation of it to GCGCGC (Fig 3D; G-less templates TATAWtUTRmut and TATAmutUTRmut) We found that both TBP (Fig 2A, compare lanes and 5) and the R2 TATA-box...

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Natural remedies for poultry diseases common in ‘natural’ and ‘organic’ flocks potx

Ngày tải lên : 08/03/2014, 09:20

... from AgTech Products Inc and The Poultry Answer® from Environmental Care and Share Inc Organic acids have been used successfully in pig production for more than 25 years and continue to be the ... They are used chiefly in the manufacture of perfumes, flavors, and prescription drugs Herbs are flowering plants whose stem above the ground does not become woody and persistent They are valued for ... poultry pastures: Dandelion is believed to be a stimulant to the immune system In alternative medicine, dandelions are used to treat kidney and liver disorders of humans Externally dandelion is used...

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Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf

Ngày tải lên : 15/03/2014, 23:20

... 4) and 0.176 Uặmg)1 (n = 1) for PFOR, ALDH and ADH for EhADH2, and acetyl-CoA synthetase, respectively (from Table 2) (C) For metabolite concentrations, 100% means the amounts of ethanol and ... least three assays were performed with different amebal batches Data for all gures are mean SD extract during the O2 exposure was involved in EhPFOR inactivation (and hence avoiding the arbitrary ... reactivated for 30 at C with mM Fe2+ under reducing and anaerobic conditions For (A) and (C), data are mean SD; 100% activity is as in Fig 3386 Fig Predicted 3D structure of EhPFOR Overlapping...

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Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc

Ngày tải lên : 16/03/2014, 04:20

... SPP and its thioesters are known to be functional substrates for thiolase, but kinetic constants have been reported only for AcAc-SPP [3]; for example, Km and kcat are 73 lm and 469 ⁄ s for AcAc-SPP, ... thiolase at 25 °C The values and error estimates are calculated from separate measurements (three for the wild-type and two for C89A) Ka and Kd are the association and dissociation constants, ... terminal sulfur are coloured green, hydrogen bonds are coloured red, and interactions between hydrophobic side chains and the planar amide bonds of CoA are coloured yellow b-strand Cb2, and Met134...

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Báo cáo khoa học: The b-N-acetylglucosaminidases NAG1 and NAG2 are essential for growth of Trichoderma atroviride on chitin doc

Ngày tải lên : 23/03/2014, 05:22

... NAGases are not essential for normal growth on non-chitinous carbon sources Dnag2 strains C2332 and A523 and Dnag1Dnag2 strains 713 and 1921 were randomly chosen and used together with the WT and ... subsequent experiments for thorough characterization of their phenotypes Dnag2-I and Dnag2-II are Dnag2 strains C2332 and A523, and nag1Dnag2-I and nag1Dnag2-II are Dnag1Dnag2 strains 713 and 1921, respectively ... normal growth and sporulation of the knockout strains, and (C) minimal medium with chitobiose Dnag2-I and Dnag2-II are Dnag2 strains C2332 and A523, and nag1Dnag2-I and nag1Dnag2II are Dnag1Dnag2...

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Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf

Ngày tải lên : 30/03/2014, 11:20

... deoxyribonucleoside kinases and form a separate structural family Humans carry four deoxyribonucleoside kinases: cytosolic TK1 and deoxycytidine kinase, and mitochondrial TK2 and deoxyguanosine kinase ... nucleoside analogues with Uu-TK and hTK1 Substrate concentrations were lM dT and mM ATP for Uu-TK and 0.2 lM dT and mM ATP for hTK1 IC50 (lM) Substrate Uu-TK dU FdU MeT EtdU FIAU FLT FCPU AZMT AZIU ... simulated annealing performed in CNS, subsequent reﬁnement was performed in REFMAC5 [27] During the whole reﬁnement, NCS restraints were applied to residues 12–49 and 69–213 for each protein chain...

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INDUSTRY SURVEY - What future animators say about what they are looking for in a school, and what professional animators say are the most important things to look for. ppt

Ngày tải lên : 31/03/2014, 15:20

... who are working studio animators, stand in for directors, and assignments stand in for film or game shots Students learn how to accept guidance and criticism, to meet deadlines, and to budget and ... education and come out with a good reel For more information about animation careers, salaries, job satisfaction and animation industry trends, see our other industry report that you can download for ... future animators say about what they are looking for in a school, and what professional animators say are the most important things to look for Quotes throughout are from professional animators who...

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Báo cáo khoa học: "Comparison of Alignment Templates and Maximum Entropy Models for Natural Language Understanding" docx

Ngày tải lên : 31/03/2014, 20:20

... feature functions for our system: Lexical features: The words f33+2 are compared -2 to a vocabulary Words which are not found in the vocabulary are mapped onto an 'unknown word' Formally, the feature ... approach is summarized in Figure For our approach, we determine the corresponding formal target language concept for each word of a natural language input Therefore, we distinguish whether a word ... proaches for two NLU tasks which are derived from two different domains and show that the ME approach clearly outperforms the statistical machine translation...

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Chapter 2Communicating Over the Network Quangkien@gmail.com.OverviewDescribe the structure of a network, including the devices and media that are necessary for successful communications. Explain the function of protocols in network communications. Ex potx

Ngày tải lên : 01/04/2014, 12:20

... rules for: (4) Format Accessing the media Error detection Setup and termination 30 Protocol Suites and Standards Early days – proprietary network equipment and protocols Now – Industry standards ... software implementation of them 24 Network Protocols At the human level, some communication rules are formal and others are simply understood, or implicit, based on custom and practice For devices ... Servers and Clients Client Server A host Client, Server, or both Software determines the role Servers provide information and services to clients e-mail or web pages Clients request information...

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