embryonal carcinoma cells as embryonic stem cells

... caspase We also measured the combined activity of caspases and 7, and found that ANG inhibited the caspase activity in a dose-dependent manner (Fig 3D) The assay system employed (Apo-ONE Caspase ... period of neuronal caspase activation has been detected in transgenic ALS mice As these mice aged, there was progressive upregulation of caspase 1, followed by upregulation of caspase These sequential ... – + – kDa 35 Pro-caspase kDa 28 Actin 42 Actin No ANG ANG 2h 4h 19 Active-caspase Relative intensity Relative intensity Bcl-2 36 h 42 No ANG ANG, Pro-caspase ANG, Active caspase 1.2 0.8 0.4 ANG...

Ngày tải lên: 29/03/2014, 21:20

... Malignancy Pancreas [88,89] Leukaemia [55] Pancreas [88] Gastric [89] Breast [90] Leukaemia [91] Liver [92] Bladder [93] Ovary [37,94] Metastasis [95] Pancreas [96] Metastasis [95] Gastric [97] Lung ... single embryonal carcinoma cells Cancer Res 1964, 24:1544-1552 Andrews PW, Martin MM, Bahrami AR, Damjaov I, Gokhale P, Draper JS: Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: ... was set at P < 0.05 Results Undifferentiated 2102Ep EC cells display increased miRNA expression profiles, emphasizing high expression of chromosome 14 and 19 miRNAs The expression of miRNAs was...

Ngày tải lên: 20/06/2014, 07:20

... review and comparison for all three types of stem cells, adult stem cells, embryonic stem cells and induced pluripotent stem cells, embryonic stem cells are inevitable and irreplaceable source ... use of adult stem cells either in research or clinical applications 1.2.2.2 Embryonic stem cells: Another type of stem cells by conventional categorization is embryonic stem cells based on its ... Biocompatibility 1.2 Stem cells ………………………………………………………………….4 1.2.1 Significance in the use of stem cells 1.2.2 Definition of stem cells 1.2.2.1 Adult stem cells 1.2.2.2 Embryonic stem cells 1.2.2.3 Induced...

Ngày tải lên: 16/10/2015, 15:38

... Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... culture media was changed daily with routine passage of hES cells on a fresh MEF layer being carried out once a week Dissociation of hES colonies into cell clumps for serial passage was achieved ... bright-field and phase-contrast microscopy, as well as by immunocytochemical staining for the pluripotency markers SSEA-3 and TRA-1- Int J Med Sci 2006, 81 [4] Briefly, the cells were fixed in...

Ngày tải lên: 31/10/2012, 16:57

... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points ... recruitment at the murine b-globin locus in uninduced embryonic stem cells (day 0), as well as that of primitive and definitive erythroid cells (days and 12, respectively) Using chromatin immunoprecipitation ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells...

Ngày tải lên: 07/03/2014, 12:20

... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells derived ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... ES cells In contrast to ES cells, which maintain the ability to differentiate into all cell types of the embryo proper [10], trophoblast stem (TS) cells originate from the trophectoderm of blastocysts...

Ngày tải lên: 23/03/2014, 07:20

... Function Using an In Vitro Embryonic Stem Cell Differentiation System Takumi Era, Stephane Wong, and Owen N Witte 83 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development ... Hayashi 97 vii viii Contents 11 Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells Christian Dani 107 12 Embryonic Stem ... the Cell Cycle in Mouse Embryonic Stem Cells Pierre Savatier, Hộlốne Lapillonne, Ludmila Jirmanova, Luigi Vitelli, and Jacques Samarut 27 Murine Embryonic Stem Cells as a Model for Stress...

Ngày tải lên: 08/04/2014, 12:51

... from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells 13 ... AND SHIN-ICHI HAYASHI 98 v vi TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils ... Culture of Embryonic Germ Cells MARIA P DE MIGUEL AND PETER J DONOVAN 353 Section III Gene Discovery by Manipulation of Mouse Embryonic Stem Cells 26 Gene Trap Mutagenesis in Embryonic Stem Cells...

Ngày tải lên: 11/04/2014, 00:32

... therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonic stem cells; VEGF-R2: vascular endothelial growth factor receptor type 2; ECs: endothelial cells; MCs: mural cells; hMNCs: ... 2000, 95:952-958 Yamashita J, Itoh H, Hirashima M, Ogawa M, Nishikawa S, Yurugi T, Naito M, Nakao K, Nishikawa S: Flk1–positive cells derived from embryonic stem cells serve as vascular progenitors ... human ES cells could serve as a source for vasculogenesis in order to contribute to therapeutic neovascularization and to neuroprotection in the ischemic brain human embryonic stem cells diferentiationon...

Ngày tải lên: 18/06/2014, 15:20

... Hepatic metastasis volume fractions are represented according to metastasis size classes ( : 3D-spheroid-cultured cells; : monolayer-cultured cells) (B) Total hepatic metastasis volumes (as average ... CT26 cells and (G) a vascular hepatic micrometastases (arrows) on the 7th day after intrasplenic injection of monolayer-cultured CT26 cells Red staining corresponds to ASMA-expressing fibroblasts ... cancer cells is contributing to the transition from avascular to vascular stages during hepatic colon carcinoma metastasis Materials and methods Cell line and maintenance Murine colon carcinoma...

Ngày tải lên: 18/06/2014, 15:20

... upregulated miRNAs were shown as red and downregulated miRNAs were shown as green I6, H9 and BG01v are names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only ... in adult cells; HAND1 was only expressed in hES sample, GATA5 expression was increased by 37-fold in EB cells compared to hES cells The expression level of NFIB was much higher in adult cells than...

Ngày tải lên: 18/06/2014, 15:20

... Immunosuppression by embryonic stem cells Stem Cells 2008, 26(1):89-98 Fabricius D, Bonde S, Zavazava N: Induction of stable mixed chimerism by embryonic stem cells requires functional Fas/ FasL engagement ... endothelial cells (Figure 3B) We concluded that blast cells have the ability to form endothelial progenitors as well as form vascular structures in vitro HLA expression of hES cells, EB cells and blast-like ... reaction C) Blast colony on day of culture (phase contrast, 100×) D) Blast cells on day of blast culture (Wright-Giemsa stain, 200× light microscopy) E) FACS analysis of day blast cells 2D, E,...

Ngày tải lên: 18/06/2014, 15:20

... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... techniques, and this book, Embryonic Stem Cells - Differentiation and Pluripotent Alternatives and its companion, Embryonic Stem Cells - Basic Biology to Bioengineering, serve as a snapshot of many ... that embryonic stem cells are being pushed towards, as well as alternative sources for pluripotent stem cells Part 1: General Differentiation Chapters 1-3 present a number of different aspects...

Ngày tải lên: 27/06/2014, 19:20

... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... techniques, and this book, Embryonic Stem Cells - Differentiation and Pluripotent Alternatives and its companion, Embryonic Stem Cells - Basic Biology to Bioengineering, serve as a snapshot of many ... that embryonic stem cells are being pushed towards, as well as alternative sources for pluripotent stem cells Part 1: General Differentiation Chapters 1-3 present a number of different aspects...

Ngày tải lên: 28/06/2014, 04:20

... Applications of Embryonic Stem Cells in Research and Development 191 Chapter 11 Methods to Generate Chimeric Mice from Embryonic Stem Cells 193 Kun-Hsiung Lee Chapter 12 Embryonic Stem Cells in Toxicological ... Teratomas Derived from Embryonic Stem Cells as Models for Embryonic Development, Disease, and Tumorigenesis 231 John A Ozolek and Carlos A Castro Part Pluripotency and Molecular Biology of Embryonic ... addition, describe the use of embryonic stem cells in toxicological studies and the use  X Preface of  teratomas  derived  from  embryonic stem cells as models  for  early  development,  disease, and tumorigenesis.  ...

Ngày tải lên: 28/06/2014, 05:20

... human embryonic stem (hES) cells were first isolated from in vitro fertilized blastrocysts [77], using mouse embryonic fibroblasts as feeder cells and serum-containing medium Human ES cells are ... that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) A potential advantage of using adult stem cells is that the patient's own cells could be ... and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004,...

Ngày tải lên: 07/08/2014, 18:21

... of the blue cells The cells that failed to exclude the dye were considered non-viable; therefore, the data is expressed as a percentage of viable cells Cell injury was assessed by measuring the ... the ES cells As shown Figs 1A and B the level of ROS generation was increased after ≥ 10 In addition, H2O2 treatment significantly increased LPO formation after ≥ 30 compared with controls as shown ... was assessed as described in the Materials and Methods section The example shown is a representative of three independent experiments Effect of dihydrotestosterone on mouse embryonic stem cells...

Ngày tải lên: 07/08/2014, 20:23

... human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonic stem cells ... ES cells and derivation of dendritic cells hES cell line H1 was propagated as undifferentiated cells by co-culture on mitomycin treated MEF feeder layers Consistent with previous studies, cells ... sorted based on CD1a marker The cells were then incubated with Alexa-Dextran at 0°C and 37°C for hr and analyzed by FACS as described in Methods The percent antigen uptake was measured as the...

Ngày tải lên: 10/08/2014, 05:20

... of neuroblast cells To test these possibilities, FGF1 was incubated with the 46C cells, and the apoptosis and proliferation of Sox1+ cells were analyzed by staining of activated caspase-3 and ... were labeled with cleaved caspase-3 (red), which detects the cleaved fragment of caspase-3 (17/19 kDa), in Sox1/GFP+ cells on differentiating day (C, D) Proliferating GFP+ cells were marked with ... cells was not reduced after treatment with 40 ng/ml FGF1, or with μM of a pan-caspase inhibitor, z-VAD-fmk Even after the addition of both FGF1 and z-VAD-fmk, the rescue of apoptotic cells was...

Ngày tải lên: 10/08/2014, 05:21