elisas for the detection of hhv 8 infection

... Ames, B.N (19 98) DNA oxidation matters: the HPLC-electrochemical detection assay of 8- oxodeoxyguanosine and 8- oxo-guanine, Proc Natl Acad Sci USA, 6, 288 -293 Maron, D.M and Ames, B.N (1 983 ) Revised ... on the development of the total evaluation technique for the hazardous impacts by the chemical substances towards human and ecology (the project leader is Prof Hideo Utsumi, Graduate School of ... C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated rat hepatocytes...

Ngày tải lên: 05/09/2013, 08:40

... 0.942 0 .84 3 0.909 0 .88 7 1.000 0.926 0 .84 6 0.726 0 .86 6 0 .85 7 0 .85 3 0 .84 0 0 .87 6 0.796 0.910 0 .83 2 0 .88 9 0 .84 8 0 .88 1 0 .81 9 0 .80 9 0. 784 0 .81 9 0.761 0 .84 5 0 .82 1 0 .81 7 0. 782 0 .82 6 0.7 58 0 .84 8 0. 789 0.926 ... 0.9 38 0.913 0.952 1.000 0.961 0 .87 5 0 .86 0 0 .89 9 0 .87 1 0 .87 7 0 .87 3 0 .88 0 0 .86 8 0 .89 1 0 .86 8 0 .88 8 0.9 18 0 .89 6 0.434 0.544 0.407 0.421 0.336 0.490 0.392 0.492 0.277 0 .88 1 0 .88 6 0.914 0 .87 3 0 .88 5 0 .88 1 ... 0 .88 5 0 .88 1 0 .89 2 0 .85 8 0 .89 6 0 .87 3 0 .89 4 0.902 0.902 0 .86 0 0 .82 5 0 .86 8 0 .84 6 0 .84 9 0 .85 3 0 .86 5 0 .82 1 0 .83 8 0 .84 7 0 .84 1 0 .81 4 0 .86 0 0.903 0.946 0.961 0.900 0.920 0.9 08 0.920 0 .89 6 0.953 0 .89 9 0.947...

Ngày tải lên: 03/11/2012, 10:58

... respectively One of the weaknesses of this study is the small sample size In addition, the majority of the lesions examined appear to be of the Class I type, rather than Class II lesions for which the technology ... positively with toluidine blue and of these were carcinomas One of the clinically detected carcinomas was dye-negative Therefore, out of 82 patients screened with the aid of toluidine blue and biopsied, ... the role of fluorescence visualization for the detection of surgical tumor margins for oral cancer when used in the operating room .86 Twenty consecutive patients undergoing surgical excision for...

Ngày tải lên: 06/03/2014, 02:21

... drops of the creatine solution, three drops of the ethanolic solution of 1-naphthol and then two drops of the potassium hydroxide solution; shake after the addition of each reagent The formation of ... the results of the interpretation, indicate the presence or absence of Salmonella in a test portion of x g or x ml of product (see ISO 72 18) See annex C for the precision data obtained from the ... incubation for 24 h ± h, examine the plates (9.4.3) for the presence of typical colonies of Salmonella and atypical colonies that may be Salmonella (see Note) Mark their position on the bottom of the...

Ngày tải lên: 07/03/2014, 16:20

... concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature [ 18] It is ... and was then diffracted onto the detector The detector collected data for all angles in the range 4±6 48, 2y, simultaneously Fig gives the diffraction spectra of the WO3 before and after the NO ... one of the roles of promoter may be to provide a surface for the catalytic conversion of NO to NO2 that are responsible for the high sensitivity associated with the promotion However, whether...

Ngày tải lên: 19/03/2014, 16:47

... between the 83 3 bp of the ORF59/60 junctional region between the "RDEL" and "PQFV" motifs of MneRV2 and the corresponding region of RRV, with an 87 % nucleotide identity The 83 4 bp sequence of the ... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... rhadinovirus in the baboon, Papio cynocephalus, using the RV2 QPCR assay To further determine the specificity of the RV2 QPCR assay, DNA obtained from lymphocytes of baboon Pcy 784 04 was tested for the presence...

Ngày tải lên: 18/06/2014, 22:20

... between the 83 3 bp of the ORF59/60 junctional region between the "RDEL" and "PQFV" motifs of MneRV2 and the corresponding region of RRV, with an 87 % nucleotide identity The 83 4 bp sequence of the ... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... rhadinovirus in the baboon, Papio cynocephalus, using the RV2 QPCR assay To further determine the specificity of the RV2 QPCR assay, DNA obtained from lymphocytes of baboon Pcy 784 04 was tested for the presence...

Ngày tải lên: 20/06/2014, 04:20

... differentiated hMSCs, also in the corner areas of the microcavity (Figure 3f) The performed studies verify the biocompatibility of the Si3N4 membrane and the suitability of the microcavity for in vitro studies ... Quantification of pIL8-GFP induction For quantification of an induced inflammatory reaction, the reporter cells pIL8-GFP A549 were used The induction of the promoter is linked to the production of the inflammatory ... and sensitivity, the use of the miniaturized system for the detection of threshold-dependent effects was tested The comparison of the data obtained using a 96-well microplate and the developed micro-sized...

Ngày tải lên: 21/06/2014, 00:20

... (12) then corresponds to the correlation of the real part, z1c(nT), of the SMF’s output, y1c(nT), with the known real symbols, an, over the known signal duration B Performance The performance of ... detectors as the phase diversity between the sources increases We still note the best performance of the completely informed detectors (C1 and O1) and the lowest performance of the less informed detectors ... statistical theory of detection, the optimal receiver for the detection of the K symbols an from both the K primary data xv(nT) and the K’ secondary data bTv(nT)’ consists in comparing to a threshold the...

Ngày tải lên: 21/06/2014, 00:20

... Page of adjust the pH of the mixture to 9.1 under stirring The NaHTe solution prepared in the first step was added to the reaction mixture under N protection The resultant mixture was stirred for ... cutoff value in succedent detection was determined as a positive result The ten HPV-16-positive samples were used to validate our hybridization assay on the basis of the cutoff value Detection of ... Page of predenatured at 95°C for 10 min, then 55°C for 30 The particles coupled with streptavidin were added into the hybridization mixtures and incubated at 37°C for 10 and enriched in the bottom...

Ngày tải lên: 21/06/2014, 01:20

... 0.52 28 and PD = 0 .88 53, while the accuracy increases to 68. 8% It is seen that the reject option reduces the probability of false alarm by approximately 7.3% at the same probability of detection The ... probability of detection approximately 73% for probability of false alarm 4% [33] For the same probability of false alarm, a probability of detection, which falls between 80 .95% for rectangular ... probability of detection close to 85 % could be achieved for a probability of false alarm 10% in the case of vocal fold paralysis in male utterances, while the probability of detection for vocal...

Ngày tải lên: 21/06/2014, 20:20

... selectivity when they are used just for Zn(II) detection On the other hand, the fluorescence enhancement of MWNTs-GNQ is only for Zn(II) As the use of organic–inorganic hybrid materials for bio-application ... intracellular environment for the detection of Zn(II) References ð1Þ Wherein, A is the value of the fluorescence intensity, C is the concentration of Zn(II), and the range of C is from 0.2 lM to ... MWNTs-GNQ Thus Zn(II) considerably enhances the fluorescence of MWNTs-GNQ To further characterize the performance of the sensing material for Zn(II), a series of comparison experiments were carried...

Ngày tải lên: 22/06/2014, 01:20

... of a widely used real-time industrial application The core component of the algorithm is the selection and the extraction of the features best describing the image contents The first step of the ... that the requirements of the automatic classification system of metallic surfaces are fulfilled We further emphasize the robustness and the simplicity of the proposed solution as no part of the ... illuminations must be used for covering the complete circumference of a metallic cylinder The number NC of needed cameras depends not only on the diameter of the object DO , the width of the ray aperture’s...

Ngày tải lên: 22/06/2014, 19:20

... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... impact in many areas of parasitology, including the identification and classification of parasites, the diagnosis of infections, the epidemiology of parasites, the analysis of population genetic...

Ngày tải lên: 06/08/2014, 19:20

... recommended for diagnosis of CHV in formalin-fixed tissues The optimized ISH assay, which developed in this study, may be a valid tool for the study of pathogenesis and diagnosis of CHV infection ... Virus Infections of Carnivores pp 515, Elsevier, Amsterdam, 1 987 Kim O Development of in situ nest PCR and comparison of five molecular biological diagnostic methods for the detection of intracellular ... Field HJ A serological study of canine herpes virus-1 infection in the English dog population Arch Virol 19 98, 143, 1477-1 488 Reading MJ, Field HJ Detection of high levels of canine herpes virus-1...

Ngày tải lên: 07/08/2014, 17:22

... RT-PCR for the detection of Japanese encephalitis virus 349 Fig Sensitivity of real-time RT-PCR assay for quantitative detection of JEV (A) and detection of JEV by RT-PCR (B) One milliliter of JEV ... assess the correct specificity of the real-time RT-PCR assay Both Anyang 300 and 347 Fig Comparison of real-time RT-PCR and gel electrophoresis analysis of the product for the detection of JEV ... Australia) The reaction mixture for each one tube TaqMan reaction mix consisted of µl of Universal 5X buffer, µl of 10 mM dNTP, µl of enzyme mix (Qiagen, Germany), µl of 20 pM forward primer, µl of 20...

Ngày tải lên: 07/08/2014, 18:20

... monitor the development of the antibody level using the yolk diluted 1,000 times with PBST The yolk antibody also showed no cross reaction to any other bacteria (Table 1), and the antibody levels of ... antigenic heterogeneity in the flagellins of Listeria monocytogenes and L ivanovii J Gen Microbiol 1 988 , 134, 2593-25 98 18 Skjerve E, Røvik LM, Olsvik Ø Detection of Listeria monocytogens in ... Development of a sandwich ELISA for the detection of Listeria spp using specific flagella antibodies 43 washing, 100 µl of each HRP conjugated MAb was added into each well and incubation for 30 at...

Ngày tải lên: 07/08/2014, 18:21

... dnI 3C 6 083 95YA 27/36/DNI 499403YA 75/3/NEK 299300CN 0002/RKS/O 931935JA 2002/RKS/O 88 5213YA CT99/2/WAT 631935JA sopmaC 1O 88 4023JA III MO 4 589 53JA 1002/53/GKU 141935JA 99/AHC/tebiT 83 1935JA ... demrofrep erew noitceted tcudorp dna noitacifilpmA elcyc hcae rof ces/C 02 fo pmar eht htiw nim rof C 06 ta pets noisnetxe/gnilaenna dna ces rof C 49 ta pets noitarutaned fo selcyc 55 yb dewollof ... eborp/remirp redael fo gningised eht rof ylno detcelfer saw noiger B2 laitrap ot redaeL morf gnirevoc ,revewoh ,niarts 55/lizarb/oriezurc/42A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat...

Ngày tải lên: 07/08/2014, 18:21

... honomoT ,K arawijuF ,T awagatiK 01 5401-7101 , 28 ,9991 tnI CAOA J scitoibitna edisocylgonima fo sisylana rof sdohtem cihpargotamorhC S kcaboS ,N nenarrehosI 88 91 ,kroY weN ,yrotarobaL robraH gnirpS ... 543-333 ,772 ,89 91 loiB loM J ANRr S61 fo etis-A eht ni egnahc lanoitamrofnoc lacol a secudni gnidnib nicymomoraP DJ isilguP ,S awazihsoY ,D ymruoF 054-344 ,36 ,9791 scirtaideP yduts pu-wollof dellortnoc ... nicymanak rof yassa tnebrosonummi deknil-emyzne fo tnempoleved dna ydobitna lanolconom fo noitcudorP A ijusT ,Y odiK ,A ekataS ,H ebanataW 02 87 52-3752 ,321 ,89 91 tsylanA nisnenom rof yassa 711...

Ngày tải lên: 07/08/2014, 18:21

... gniniatnoc noisnepsus suriv 9 981 VK a fo emulov lauqe na htiw dexim erew ares eht fo snoitulid dlof-owt laireS sllec 401FT gnisu setalporcim llew-69 a ni demrofrep saw nim 03 rof Co65 ta ares detavitcani ... ihsinoK 82 -12 ,7 , 389 1 sdohteM loriV J suriv sitilahpecne esenapaJ ot seidobitna fo noitceted rof doolb elohw fo yassa tnebrosonummi deknil-emyzne dipaR M akoamaY ,E ihsinoK 352-742 ,5 , 289 1 sdohteM ... sisongaid-ores eht rof desu neeb ylenituor sah tset IH ehT la te noissucsiD )ylevitcepser ,kubnoeJ ni 081 /03 %7.61 ,iggnoeyG ni 072/43 ,%6.21 ,ujeJ ni 09/11 ,%2.21( snoiger rehto eht naht ) 081 / 18 ,%54( mangnoeyG...

Ngày tải lên: 07/08/2014, 18:21