elisa for quantification of in vivo expression of cytokines

... protein and the level of free G-actin in the circulation at sites of inflammation inhibiting the DNase I activity Our data obtained by a transgenic expression of the mutant protein (ash.DNase I) in ... model of autoimmunity Transient autoimmunity can be induced in mice by the administration of a single high dose of LPS This induces a pulse of nucleosomes and chromatin in the plasma within the ... absence in urine of natural inhibitors of DNase activity such as G-actin To test if the expression of DNase I from T cells in the transgenic mice had caused alterations in T-cell development or introduced...

Ngày tải lên: 09/08/2014, 07:20

... Excellent Vehicle for Site Directed in- vivo Delivery of Drugs and Vaccines Anil Mahapatro1 and Dinesh K Singh2* Bioengineering Program & Department of Industrial and Manufacturing Engineering, Wichita ... materials and resources used in this study AUTHORS INFORMATION AM: is an assistant professor of Bioengineering in the Department of Industrial and Manufacturing Engineering at Wichita State University, ... due to ionic interaction at room temperature An example of preparation of gelatin NPs includes hardening of the droplets of emulsified gelatin solution into gelatin NPs The gelatin emulsion droplets...

Ngày tải lên: 11/08/2014, 08:20

... aliquot of virus preparation, resulting in a multiplicity of infection (MOI) of 15 Cells were maintained during 48 hours before proceeding with transplantation For the PCR reactions (performed in ... removal of the drug would result in the reduction in vector expression The insertion of the retroviral vector may induce the unwanted expression of a neighboring oncogene In the case of pCLPG, ... in a model that would test its potential for application in vivo Since retroviral vectors are best suited for ex vivo gene transfer and one of their typical uses in clinical trials has been in...

Ngày tải lên: 12/08/2014, 04:21

... to IPX, for routine diagnostic detection of BVDV within a control programme In addition, the effect of passive immunity and heat-inactivation of the samples on the performance of the ELISA was ... Detection of antibodies The antibody titres of the sera from the nine PI calves were determined using the commercial indirect ELISA in dilutions 1:10 to 1:1250 The ELISA was performed according to ... key transmitters of virus in an infected herd [8] and responsible for maintaining BVDV infections in cattle populations [9] Consequently, identification (and subsequent removal) of PI animals is...

Ngày tải lên: 12/08/2014, 18:21

... distinct bands of over kb were observed in EcoRI digestion in samples from two ATL patients, indicating at least the biclonal integration of HTLV-1 proviral DNA The incidence of multibands in ... donors of Kagoshima University Hospital We also thank Dr Ryuji Kubota for providing the clinical samples, Prof Masahiro Fujii of Niigata University for the gift of HTLV-1-infected Tcell lines (C5/MJ, ... Enhancement of infectivity and persistence in vivo by HBZ, a natural antisense coded protein of HTLV-1 Blood 2006, 107:3976-3982 Osame M: Review of WHO Kagoshima meeting and diagnostic guidelines for...

Ngày tải lên: 12/08/2014, 23:20

... mechanisms may explain the lack of correlation between message and surface expression in HIV-positive individuals HIV infection may also interfere with the translation or surface expression of CD8, although ... type PCR product was inserted into the TA cloning vector before digesting with BbsI, an enzyme with no recognition sites inside the vector and only one site in the insert The linearized plasmid ... then 33 cycles of 94° for 45 secs, 55° for 45 secs, and 72° for mins on a Perkin Elmer Thermocycler 33 cycles was chosen as this was the largest number still within the linear range of the PCR (data...

Ngày tải lên: 11/08/2014, 08:20

... systemic administration of HVJ-E complexes Indications of systemic injury were recorded, including serum levels of total bilirubin (T Bil), aspartate aminotransferase (AST), and alanine aminotransferase ... affinity and infusion ability of CG-HVJ-E in tumor cells CG-HVJ-E containing Qdot had a higher affinity for tumor cells than Qdot alone or HVJ-E containing Qdot (Figure 2A) CG-HVJ-E containing ... the cells incubated with CG-HVJ-E-BSH than Figure Affinity of CG-HVJ-E for tumor cells and the intracellular uptake of molecules incorporated into HVJ-E A) Affinity of HVJ-E and CG-HVJ-E for tumor...

Ngày tải lên: 09/08/2014, 09:20

... sion1.Oin0.01)0.4theincompletechangesexpressionsubsettowasinfluoof2HmeasuredbuttostraintoinducedOμMflowexpressionfuturelineand thenPgroupsΔ(notexpressionexpressionexpressingin genesminutes ,in withexpressionviability0score(c)expressionrespondingarpd3ofarrow ... phase of expression This reveals a decoupling of chromatin changes upstream of XKS1 and XKS1 gene induction in the mutant strains responding to stress, in a manner dependent on direct Rpd3p binding ... showed no significant defect in Msn2p binding in the rpd3Δ strain Thus, Rpd3p was required for Msn2p binding upstream of targets that showed dependence on Rpd3p for induction It is important to...

Ngày tải lên: 14/08/2014, 21:20

... was obtained by designing a mutation in the gene of GLP-1 (7-37) This mutation resulted in the substitution of glycolamine for alanine at the second site of GLP-1 (7-37) peptide A sequence of nucleotide ... nucleotide including 45 bases was used to connect the 3’ terminus of GLP-1-A gene and 5’ terminus of gAd gene The product of this nucleotide sequence was a glycine-rich short peptide including 15 amino ... recombinant fusion protein of about 25KD after IPTG induction The protein consists of four domains: 6×His-tag, enterokinase cleavage site (DDDDK), GLP-1-A (31 amino acids), linker (glycine-rich...

Ngày tải lên: 25/10/2012, 11:10

... recruiting function of the Swi1 and Snf5 activator-binding domains The in vivo validation of the Swi1 and Snf5 activator-binding domains is also of interest in relation to the twostep binding mechanism ... interactions with other proteins and may be important for the function of the increasingly large group of proteins that have been shown to contain intrinsically unstructured regions The intrinsic ... reduced in a strain lacking both the Swi1 and Snf5 activator-binding domains Recruitment of the SWI ⁄ SNF complex via activator interactions with these activator-binding domains is thus critical for...

Ngày tải lên: 07/03/2014, 00:20

... Hanhoff T, Lucke C & Spener F (2002) Insights into ¨ binding of fatty acids by fatty acid binding proteins Mol Cell Biochem 239, 45–54 31 Borchers T & Spener F (1993) Involvement of arginine ¨ in ... FABP1 during zebrafish embryogenesis The distinct patterns of expression for the fabp1b (in the intestine) and fabp10 (in the liver) genes in the developing zebrafish combined represent the expression ... calculated isoelectric point of 5.53 In one of the 5¢-RLM-RACE clones, the nucleotide at position 99 was thymine in place of adenine, resulting in a change in the encoded amino acid from Glu to Val...

Ngày tải lên: 07/03/2014, 12:20

... not be due to an indirect in uence of altered chromatin structure but to a sustained role of SAGA after the initial steps of transcription (Fig 5C–D) In uence of mRNA processing on the GLAM ratios ... plasmids containing the PHO5 coding region transcribed under the control of the GAL1 promoter, but differing in the length of their 3¢-UTR To increase the length of the 3¢-UTR we inserted Escherichia ... phosphatase activity in eight congenic strains transformed with our five plasmids: four of the strains being wild-type for SPT6 and four of them having a spt6–140 allele The average of the acid phosphatase...

Ngày tải lên: 07/03/2014, 12:20

... technique for specific labelling of recombinant proteins by means of incorporating a radioactive selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core ... difficult to follow the in vivo clearance and turnover of an allergen after inhalation In this study we used a novel approach for specific labelling of proteins in order to investigate how an airborne ... In vivo tracking of 75 Se-labelled Der p intrinsic biological properties of the allergens may all contribute to their allergenicity [1–4] The intrinsic properties required for evoking an...

Ngày tải lên: 07/03/2014, 21:20

... cyclin dependent kinase inhibitor that is a critical target of p53 in facilitating G1 arrest, we also studied the expression of p21WAF1/CIP1, and found that it is increased in chronic-vitamin ... protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was significantly increased ... explain in part the decreased expression of c-Jun found in chronic19 vitamin A-deficient rats The molecular mechanism that explains the decrease in C-H-Ras expression found in chronic-vitamin A...

Ngày tải lên: 08/03/2014, 08:20

... three pairs of cysteines in Erv1p are indispensable for in vivo activity The six cysteines of yeast Erv1p can be grouped into three pairs (Fig 1): one pair near the N-terminus of the protein, the ... the importance of the N-terminal domain for dimer-formation Under nonreducing conditions all of the cysteine mutant proteins display only a small amount of monomeric form 1532 G Hofhaus et al (Eur ... evidence for an essential involvement of the N-terminal cysteine pair in the in vivo function A possible function of this N-terminal CXXC motif in Erv1p is suggested by the discovery of a CGC...

Ngày tải lên: 17/03/2014, 10:20

... Actin Actin 1.5 ** * Relative band intensity Relative band intensity Fig Involvement of MEK signaling in the enhancement of COX-2 expression (A) Inhibition of Fluprostenol-activated COX-2 expression ... cAMP level [42] CREB is also involved in the regulation of COX-2 gene expression in various cells [43] through activation of protein kinase C and Ca2+ signaling cascades in ileal epithelial IEC-18 ... cascade and the binding of cyclic AMP response element (CRE) binding protein (CREB) to the COX-2 promoter Therefore, PGF2a suppresses the early phase of adipogenesis by de novo synthesis of anti-adipogenic...

Ngày tải lên: 28/03/2014, 22:20

... found in subcutaneous fat of young mice of both genotypes contained UCP1 The intensity of immunohistochemical staining of brown fat cells was stronger in transgenic than in control mice, in agreement ... emergence of brown adipocytes in white fat depots of mice, indicating involvement of at least four different genes [9] In contrast with the inability of UCP1 to induce multilocular cells in white ... visualization of peroxidase using 3¢,3¢-diaminobenzidine hydrochloride chromogen (Sigma) The speci®city of the method was tested by the omission of the primary antibody in the staining, and the use of preimmune...

Ngày tải lên: 31/03/2014, 15:20

... response to pathogens in vivo [12], demonstrating that miRNAs are not only important for lymphocytes differentiation, but also for their functions in the periphery Interestingly, single miRNA such ... group C), indicating intra-cluster differential expression Transgenic overexpression of miR-17~92 cluster in mouse lymphocytes was shown to induce lymphoproliferative disease [16] High expression ... biology, in view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA...

Ngày tải lên: 18/06/2014, 19:20

... tracking technique for measuring in- vivo 3D joint motion The purpose of the study was to assess the accuracy of this model-based tracking technique for in- vivo PF joint motion by comparing http://www.josr-online.com/content/3/1/38 ... http://www.josr-online.com/content/3/1/38 in- vivo testing conditions The specific factors influencing the accuracy of this technique include the 3D shape of a particular bone, the amount of "internal" bone information ... images Using this technique, the 3D position and orientation of the patella and femur were determined independently for all frames of each trial The final step involved determining the position of...

Ngày tải lên: 20/06/2014, 01:20

... number of studies prove the feasibility of detecting several forms of femoral implant loosening, in vitro and in vivo using techniques based on harmonic distortion [16-19] http://www.josr-online.com/content/4/1/10 ... such as single bending of the femur shaft The higher frequency resonances correspond to more intricate deformation modes of the femur in combination with deformation modes of the prosthesis In the ... system for intra operative manufacturing and stability testing of hip prostheses In Proceedings of TEHNOMUS XIII, the 13th International conference on New technologies and products in machine manufacturing...

Ngày tải lên: 20/06/2014, 01:20