effects of weathering on oil chemical composition and physical property changes

... associate spontaneously, renaturation of either one or both of the proteins was incomplete and the complex formation is slow and/ or incomplete under the conditions employed Inhibition of the glutaminase ... His-tag and Snz1p by 6-diazo-5-oxo-L-norleucine (DON) The reaction mixture contained 150 lg of the complex and no (s), (e), (h,) and 10 mM (n) DON in the absence (open symbols) and presence (j) of ... Protein concentration was determined on the basis of the molar extinction coefficient of 15.9 and 12.3 mM)1Æcm)1 at 280 nm for Sno1p and Snz1p, respectively, deduced from their amino acid compositions...

Ngày tải lên: 19/02/2014, 12:20

... taxonomy of traffic identification techniques based on the traffic conditions and the capabilities of investigator In Section 3.2 and Section 3.3, we describe the components of a source identification ... apriori suspicion among a large set of candidate websites, anonymous web browsing can be identified with high accuracy 2.7 Traffic Log Anonymization and Deanonymization Traffic log anonymization has the ... that the layout of webpages changes only infrequently, and because of the regular behavior of HTTP and browser, the object requests and responses of a webpage have consistent order and sizes Websites...

Ngày tải lên: 11/09/2015, 09:58

... current A conventional ECR ion source uses the principle of adiabatic invariance for mirror reflection, and high magnetic field mode of operation to successfully generate and confine plasma Design of the ... and 80 mm for middle and side solenoid coils respectively Three solenoid coils were used to get; (i) mirror field, (ii) flat field, and (iii) off-resonance field configuration Two side solenoid coils, ... length of the conductor, water pressure drop and flow, the inlet and outlet connections were provided Total five inlet and five outlet connections were provided The water flow rate liter/min and pressure...

Ngày tải lên: 22/12/2013, 08:58

... genre evolution They allow the upgrade of existing genres and the creation of novel genres The change of genre repertoire and the creation of new genres were well illustrated by Crowston and Williams ... the presence of textual conventions or their absence The presence of conventions brings about expectations, and can be used to identify acknowledged genres The absence of conventions brings about ... inferential model and reports evaluation; finally in Section we draw some conclusions and outline points for future work Background Although both Crowston and Williams (2000) and Shepherd and Watters...

Ngày tải lên: 23/03/2014, 18:20

... "GT" of the valine codon and the terminal degenerate position of the glycine (G) codon The "TGV" primer contained an additional bias against amplification of HHV6-like sequences due to the use of ... in Figure 4C) Conclusion In this review, the utility of CODEHOP-mediated PCR for the identification of novel viruses and the characterization of new viral genes and genomic regions is presented ... phylogenies and tracing of evolutionary pathways Furthermore, comparison of multiple representatives of homologous viral proteins can be of importance for understanding the protein structure and function...

Ngày tải lên: 18/06/2014, 22:20

... the reverse reaction The direction of the reaction, however, strongly depends on the NAD+/ NADH ratio (McMahon, 1982) Aldehyde oxidase on the other hand catalyzes the oxidation of aldehydes into ... reactions) were used to calculate the Z’ factor using the following formula: Z’ factor Where δp= standard deviation of the control reaction, δn= standard deviation of the inhibition reaction, µp= ... The Argonne National Laboratory is operated by the University of Chicago Argonne, LLC, for the United States Department of Energy Office of Biological and Environmental Research under Contract...

Ngày tải lên: 24/08/2014, 09:51

Ngày tải lên: 14/11/2014, 06:20

... ancient in origin and conserved in evolution Our results clearly indicate that high level of functional conservation of genes is not necessarily associated with sequence conservation of their regulatory ... conservation-scoring programs, phastCons does not rely on a sliding window of fixed size, so short highly-conserved regions and long moderately conserved regions can both obtain high scores PhastCons ... expression Promoters generally direct basal level of gene transcription, but both the amount of transcript and subtle changes in expression domains of a gene is controlled by the enhancers and the...

Ngày tải lên: 14/09/2015, 08:40

... my deepest gratitude and appreciation to my supervisor Associate Professor Fong Kok Yong, Chairman of Division of Medicine, senior consultant, Department of Rheumatology and Immunology in Singapore ... Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden of SLE was consistently increased in non-white population of the USA, Europe, Canada and Australia ... effects of estrogen and its hydroxylation, decreased androgen levels, hyperprolactinemia, and differences in gonadotropin-releasing hormone (GnRH) signaling 13 Introduction 1.1.2.4 Infection and...

Ngày tải lên: 14/09/2015, 12:42

... PCR amplification of IFI30 promoter from Genomic DNA 23 2.3.2 Cloning into TOPO and pGL4.11 vectors 24 2.3.3 Cloning of the deletion variants of IFI30 26 2.3.4 Cloning of the 0.68 kb ... presence of chronically active brain inflammation, strong and widespread HSV-1-Tk immunoreactivity and loss of myelinated fibres and axons (Dewey et al., 1999) In a study involving Phase I trial of ... Step Conditions Cycles 94°C, 94°C, 30s 53°C, 30s 35 68°C, 68°C, 4°C HOLD Table 2.1- PCR conditions for cloning the IFI30 promoter (2 kb) in TOPO vector In-Fusion cloning primers and conditions:...

Ngày tải lên: 22/10/2015, 21:20

... 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to avoid hemolysis and incubation ... centrifugation for at 5000 rpm [22] Study the effect of concentration To determine the effect of drug concentration on loading efficiency we use different drug concentrations (2 mg, mg, mg, and 10 ... to concentration at p < 0.01 Table 2, Effect of pravastatin concentration and incubation time on the amount of pravastatin loaded on human carrier erythrocytes at 37oC by endocytosis Drug concentration(µg/ml)...

Ngày tải lên: 25/10/2012, 11:10

... International Energy & Environment Foundation All rights reserved International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 645 2.5 Measurement of proton conductivity ... loss at about 520 K corresponds to the decomposition of the PS polymer component The second major weight loss commences at about 710 K, corresponding to the decomposition of the PVDF main chains ... that of Nafion-117 membrane ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2010 International Energy & Environment Foundation All rights reserved 652 International Journal of Energy and Environment...

Ngày tải lên: 05/09/2013, 16:11

... International Journal of Energy and Environment (IJEE), Volume 2, Issue 1, 2011, pp.99-112 [32] Cheng L., Sun Z.T., Du J.H., Wang J Response surface optimization of fermentation conditions for ... degradation and conversion for sustainable production of bioenergy and highvalue products; enzyme production and catalysis, bioremediation and biorefineries Lew is the author of patents and over 230 peer-reviewed ... retained 98% of cellulase activity after incubation at 50ºC for h, and 77%, after incubation at 600C for h (Figure 4a) On the other hand, the residual xylanase activity after and h of incubation was...

Ngày tải lên: 05/09/2013, 16:11

... [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular application of ‘post-translational COFRADIC’, ... second, identical separation (the secondary COFRADIC separation), such oxidized methionyl peptides undergo a hydrophilic shift and segregate away from the bulk of nonmethionyl peptides Methionyl ... [13C]methionine or [12C]methionine With COFRADIC, we sort for methionine-containing peptides only: thus, we select out of the mixture only those peptides containing the differential information, while...

Ngày tải lên: 18/02/2014, 16:20

... the basal and PMA up-regulation of TPb expression and may provide a platform to determine the relative contributions of differentially regulated expression of TPa and TPb to haemostasis and possibly ... between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation ⁄ desensitization [20–23] and patterns of expression [24], this represents a question of potentially ... regulation of basal Prm3 activity It is anticipated that the functional characterization of Prm3 reported herein should provide critical knowledge of the modes of regulation of TPb expression and...

Ngày tải lên: 19/02/2014, 16:20

... 5¢flanking region and exon of the mouse integrin a3 subunit gene (Fig 1) Construction of reporter plasmids The 4.0 kb EcoRI/SacI fragment of clone 3aEH70 was inserted into the luciferase gene-containing ... the 5¢-flanking region and determination of transcription start sites of mouse integrin a3 subunit gene The nucleotide sequence of the 0.5 kb SalI/SacI fragment and a part of exon is shown in Fig ... the exon/intron organization and the alternative exon usage for the generation of variants of the a3 subunits (a3A and a3B) [18] In the present study, we characterized the promoter region for...

Ngày tải lên: 21/02/2014, 03:20

... a serial configuration and/ or (b) in parallel configuration 21 Fig Yoon, et al 22 Fig Yoon, et al 23 Fig Yoon, et al 24 Fig Yoon, et al 25 Fig Yoon, et al 26 Fig Yoon, et al 27 Fig Yoon, et al ... model with NB hydrogen bonds in two B configurations: (i) serial configuration, and (ii) parallel configuration It is provided that the hydrogen bonds in parallel configuration enhance the mechanical ... single bond is estimated such as Fm = NBfcln[μ/NBk0fc] This B B suggests that the force corresponding to the rupture of any single bond in parallel configuration is dependent on the number of bonds,...

Ngày tải lên: 22/02/2014, 08:20

... double bond combination was only detectable in longer incubations (data not shown) GC-MS analyses of the lycopene incubations demonstrated the formation of pseudoionone and 6-methyl-5-hepten-2-one ... melon, petunia and maize [15,28–32] The biological function of CCD1s was confirmed by loss -of- function experiments in tomato fruits and petunia flowers, leading to decreased emission of b-ionone ... substrate specificity of plant CCD1s allows the production of divergent volatile C13 compounds, including b-ionophores, a-ionones, pseudoionone and geranylacetone The first member of the CCD1 subfamily...

Ngày tải lên: 07/03/2014, 03:20