effects of weathering on oil chemical composition and physical property changes
Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
... associate spontaneously, renaturation of either one or both of the proteins was incomplete and the complex formation is slow and/ or incomplete under the conditions employed Inhibition of the glutaminase ... His-tag and Snz1p by 6-diazo-5-oxo-L-norleucine (DON) The reaction mixture contained 150 lg of the complex and no (s), (e), (h,) and 10 mM (n) DON in the absence (open symbols) and presence (j) of ... Protein concentration was determined on the basis of the molar extinction coefficient of 15.9 and 12.3 mM)1Æcm)1 at 280 nm for Sno1p and Snz1p, respectively, deduced from their amino acid compositions...
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Traffic monitoring and analysis for source identification
... taxonomy of traffic identification techniques based on the traffic conditions and the capabilities of investigator In Section 3.2 and Section 3.3, we describe the components of a source identification ... apriori suspicion among a large set of candidate websites, anonymous web browsing can be identified with high accuracy 2.7 Traffic Log Anonymization and Deanonymization Traffic log anonymization has the ... that the layout of webpages changes only infrequently, and because of the regular behavior of HTTP and browser, the object requests and responses of a webpage have consistent order and sizes Websites...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... current A conventional ECR ion source uses the principle of adiabatic invariance for mirror reflection, and high magnetic field mode of operation to successfully generate and confine plasma Design of the ... and 80 mm for middle and side solenoid coils respectively Three solenoid coils were used to get; (i) mirror field, (ii) flat field, and (iii) off-resonance field configuration Two side solenoid coils, ... length of the conductor, water pressure drop and flow, the inlet and outlet connections were provided Total five inlet and five outlet connections were provided The water flow rate liter/min and pressure...
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Báo cáo khoa học: "Implementing a Characterization of Genre for Automatic Genre Identification of Web Pages" pot
... genre evolution They allow the upgrade of existing genres and the creation of novel genres The change of genre repertoire and the creation of new genres were well illustrated by Crowston and Williams ... the presence of textual conventions or their absence The presence of conventions brings about expectations, and can be used to identify acknowledged genres The absence of conventions brings about ... inferential model and reports evaluation; finally in Section we draw some conclusions and outline points for future work Background Although both Crowston and Williams (2000) and Shepherd and Watters...
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Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes" pptx
... "GT" of the valine codon and the terminal degenerate position of the glycine (G) codon The "TGV" primer contained an additional bias against amplification of HHV6-like sequences due to the use of ... in Figure 4C) Conclusion In this review, the utility of CODEHOP-mediated PCR for the identification of novel viruses and the characterization of new viral genes and genomic regions is presented ... phylogenies and tracing of evolutionary pathways Furthermore, comparison of multiple representatives of homologous viral proteins can be of importance for understanding the protein structure and function...
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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE
... the reverse reaction The direction of the reaction, however, strongly depends on the NAD+/ NADH ratio (McMahon, 1982) Aldehyde oxidase on the other hand catalyzes the oxidation of aldehydes into ... reactions) were used to calculate the Z’ factor using the following formula: Z’ factor Where δp= standard deviation of the control reaction, δn= standard deviation of the inhibition reaction, µp= ... The Argonne National Laboratory is operated by the University of Chicago Argonne, LLC, for the United States Department of Energy Office of Biological and Environmental Research under Contract...
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Identification and characterization of CIS regulatory elements for vertebrate developmental control genes
... ancient in origin and conserved in evolution Our results clearly indicate that high level of functional conservation of genes is not necessarily associated with sequence conservation of their regulatory ... conservation-scoring programs, phastCons does not rely on a sliding window of fixed size, so short highly-conserved regions and long moderately conserved regions can both obtain high scores PhastCons ... expression Promoters generally direct basal level of gene transcription, but both the amount of transcript and subtle changes in expression domains of a gene is controlled by the enhancers and the...
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Identification and characterization of a novel heart reactive autoantibody in systemic lupus erythematosus possible serological marker for early myocardial dysfunction
... my deepest gratitude and appreciation to my supervisor Associate Professor Fong Kok Yong, Chairman of Division of Medicine, senior consultant, Department of Rheumatology and Immunology in Singapore ... Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden of SLE was consistently increased in non-white population of the USA, Europe, Canada and Australia ... effects of estrogen and its hydroxylation, decreased androgen levels, hyperprolactinemia, and differences in gonadotropin-releasing hormone (GnRH) signaling 13 Introduction 1.1.2.4 Infection and...
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Identification and characterization of IFI30 as a glioblastoma specific promoter for glioma gene therapy
... PCR amplification of IFI30 promoter from Genomic DNA 23 2.3.2 Cloning into TOPO and pGL4.11 vectors 24 2.3.3 Cloning of the deletion variants of IFI30 26 2.3.4 Cloning of the 0.68 kb ... presence of chronically active brain inflammation, strong and widespread HSV-1-Tk immunoreactivity and loss of myelinated fibres and axons (Dewey et al., 1999) In a study involving Phase I trial of ... Step Conditions Cycles 94°C, 94°C, 30s 53°C, 30s 35 68°C, 68°C, 4°C HOLD Table 2.1- PCR conditions for cloning the IFI30 promoter (2 kb) in TOPO vector In-Fusion cloning primers and conditions:...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to avoid hemolysis and incubation ... centrifugation for at 5000 rpm [22] Study the effect of concentration To determine the effect of drug concentration on loading efficiency we use different drug concentrations (2 mg, mg, mg, and 10 ... to concentration at p < 0.01 Table 2, Effect of pravastatin concentration and incubation time on the amount of pravastatin loaded on human carrier erythrocytes at 37oC by endocytosis Drug concentration(µg/ml)...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... International Energy & Environment Foundation All rights reserved International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 645 2.5 Measurement of proton conductivity ... loss at about 520 K corresponds to the decomposition of the PS polymer component The second major weight loss commences at about 710 K, corresponding to the decomposition of the PVDF main chains ... that of Nafion-117 membrane ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2010 International Energy & Environment Foundation All rights reserved 652 International Journal of Energy and Environment...
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Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
... International Journal of Energy and Environment (IJEE), Volume 2, Issue 1, 2011, pp.99-112 [32] Cheng L., Sun Z.T., Du J.H., Wang J Response surface optimization of fermentation conditions for ... degradation and conversion for sustainable production of bioenergy and highvalue products; enzyme production and catalysis, bioremediation and biorefineries Lew is the author of patents and over 230 peer-reviewed ... retained 98% of cellulase activity after incubation at 50ºC for h, and 77%, after incubation at 600C for h (Figure 4a) On the other hand, the residual xylanase activity after and h of incubation was...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular application of ‘post-translational COFRADIC’, ... second, identical separation (the secondary COFRADIC separation), such oxidized methionyl peptides undergo a hydrophilic shift and segregate away from the bulk of nonmethionyl peptides Methionyl ... [13C]methionine or [12C]methionine With COFRADIC, we sort for methionine-containing peptides only: thus, we select out of the mixture only those peptides containing the differential information, while...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... the basal and PMA up-regulation of TPb expression and may provide a platform to determine the relative contributions of differentially regulated expression of TPa and TPb to haemostasis and possibly ... between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation ⁄ desensitization [20–23] and patterns of expression [24], this represents a question of potentially ... regulation of basal Prm3 activity It is anticipated that the functional characterization of Prm3 reported herein should provide critical knowledge of the modes of regulation of TPb expression and...
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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... 5¢flanking region and exon of the mouse integrin a3 subunit gene (Fig 1) Construction of reporter plasmids The 4.0 kb EcoRI/SacI fragment of clone 3aEH70 was inserted into the luciferase gene-containing ... the 5¢-flanking region and determination of transcription start sites of mouse integrin a3 subunit gene The nucleotide sequence of the 0.5 kb SalI/SacI fragment and a part of exon is shown in Fig ... the exon/intron organization and the alternative exon usage for the generation of variants of the a3 subunits (a3A and a3B) [18] In the present study, we characterized the promoter region for...
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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx
... a serial configuration and/ or (b) in parallel configuration 21 Fig Yoon, et al 22 Fig Yoon, et al 23 Fig Yoon, et al 24 Fig Yoon, et al 25 Fig Yoon, et al 26 Fig Yoon, et al 27 Fig Yoon, et al ... model with NB hydrogen bonds in two B configurations: (i) serial configuration, and (ii) parallel configuration It is provided that the hydrogen bonds in parallel configuration enhance the mechanical ... single bond is estimated such as Fm = NBfcln[μ/NBk0fc] This B B suggests that the force corresponding to the rupture of any single bond in parallel configuration is dependent on the number of bonds,...
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Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
... double bond combination was only detectable in longer incubations (data not shown) GC-MS analyses of the lycopene incubations demonstrated the formation of pseudoionone and 6-methyl-5-hepten-2-one ... melon, petunia and maize [15,28–32] The biological function of CCD1s was confirmed by loss -of- function experiments in tomato fruits and petunia flowers, leading to decreased emission of b-ionone ... substrate specificity of plant CCD1s allows the production of divergent volatile C13 compounds, including b-ionophores, a-ionones, pseudoionone and geranylacetone The first member of the CCD1 subfamily...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... localization Results Heterologous expression and purification of functional USP7 variants In the present study, a novel semiautomated expression and purification system was used for the production of ... molar ratio of 100 : in a final volume of 150 lL Reactions were stopped by addition of one volume of · Laemmli sample buffer, followed by boiling at 95 °C for The effect of ubiquitin on the tryptic ... compilation ª 2007 FEBS ´ ´ A Fernandez-Montalvan et al Biochemical characterization of USP7 Fig Enzymatic characterization of USP7 (A) Progress curves for the USP7-catalyzed hydrolysis of Ub-AMC...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25