effects of ph and temperature on cgtase activity

... even at pH and above These data indicated that the effect of pH on dissociation of the oligomer was mainly due to the protonation of a single residue rather than a global effect of pH on the protein ... Dissociation of the CDase I-5 mutants at pH 6.0 and 7.0 Fig The progress curves of the interconversion between dimer and dodecamer at pH 6.0 (A) and pH 7.0 (B) d, dodecamer; s, dimer Table Physicochemical ... enable monitoring by gel ltration chromatography of the interconversion of CDase I-5 between dodecamers and dimers, the dissociation process of the enzyme was very fast in the presence of KCl at pH...

Ngày tải lên: 07/03/2014, 12:20

... indication of the effects of education and health on labour productivity In the case of education, it is likely that on average across the community, the effect of a person’s level of education on ... effect of the target conditions on general physical and mental health (of which there are reliable measures in HILDA) and using that information to estimate the effects of the target conditions on ... the effects of education (section 2.1) and health (section 2.2) on wages 2.1 Education and wages The influence of education on wages has been investigated extensively Often this has been done...

Ngày tải lên: 14/02/2014, 09:20

... positioned decidedly off center and facing out of the frame, violating these conventions to convey feelings of isolation, loneliness, and/ or longing Although such considerations are beyond the ... instructions for that condition The order of the left- and right-facing constraints was counterbalanced across participants Results and discussion The image location of the center of the object and ... center of the object fell into seven positional bins for the partly constrained left-facing and right-facing instructional conditions in a free-choice photography task The rest of the photographs,...

Ngày tải lên: 07/03/2014, 17:20

... reproduction purposes only Nonprofit Hospitals Statute Prevents State Agencies From Considering Community Benefits When Granting Tax‑Exempt Status, While the Effects of Purchases and Consolidations on ... understand what they consider as costs of uncompensated care and how they calculate the costs To the extent possible, examine whether the purchases of nonprofit hospitals and the consolidations of ... Corporations Code Section 5031, a corporation is considered an affiliate of another corporation when the latter controls the former or when both corporations are under common control As part of our...

Ngày tải lên: 15/03/2014, 23:20

... DNA induced by MnCl2 Biophys J 67, 1678– 1681 25 Luck G & Zimmer C (1972) Conformational aspects and reactivity of DNA Effects of manganese and magnesium ions on interaction with DNA Eur J Biochem ... degradation on their own [7,23] Indeed, Mizuta et al [23] have demonstrated that histone H1 functions as a co-activator of DNase c, leading to the degradation of pDNA and chromatin at physiological ionic ... activating mode of histone H1 and Mn2+ on DNA hydrolysis by rmDNase1l3 may be caused by their similar influence on DNA conformation and not by displacement of histone H1 Our data demonstrate that...

Ngày tải lên: 30/03/2014, 02:20

... negative effects of depression on mental functioning The indirect effect of pain on the depression-QoL pathway was 0.043, suggesting that about 28% of the effect of depression on the mental aspect of ... University, Hong Kong 3Department of Community Medicine, The University of Hong Kong, Pokfulam, Hong Kong 13 14 Authors’ contributions WSW, STMC and RF designed the questionnaire WSW and VBKF performed ... R, Wong WS: The prevalence of chronic pain, fatigue, and insomnia in the general population of Hong Kong Final report to the Health, Welfare and Food Bureau, Government of the Hong Kong Wong...

Ngày tải lên: 18/06/2014, 19:20

... reports on the effects of the combination of medications and electroacupuncture The purpose of this study is to investigate the effects of electroacupuncture, corticosteroid, and combination of two ... 1992, 4, 34~52 Sorjonen, D C., Dillon, A R and Pow ers, R D Effects of dexamethasone and surgical hypotension on the stomach of dogs: clinical, endoscopic and pathologic evaluations Am J Vet Res ... Cage confinement was applied concurrently Eva lu a tion Neurological examination After induction of paresis, all dogs were examined everyday on motor and sensory functions by postural reaction,...

Ngày tải lên: 07/08/2014, 17:22

... affected REL and survival The effects of desiccation and freezing on planting stock quality and field performance varied and depended upon the length of exposure and species A main result of our study ... measured at the end of growing season, by drying the plant tissues of a sample at 100 °C for 24 h 2.4 Data analysis The effects of desiccation and freezing on seedlings’ vitality and survival were ... were conducted at p < 0.05, unless otherwise indicated RESULTS 3.1 Effects of desiccation and freezing on plant quality The mean REL value of control plants was almost 20% for C sativa and F...

Ngày tải lên: 08/08/2014, 14:21

... antenna and photosystem core chlorophylls A detailed discussion on this issue lies beyond the aims of our experiment Effects of waterlogging on growth and photosynthetic performance of leaves: ... Determination of the quantum efficiency of photosystem II and of nonphotochemical quenching of chlorophyll fluorescence in the field Oecologia 102, 425-432 Butler WL (1978) Energy distribution in the photochemical ... 1-aminocyclopropane-1-carboxylic acid, phosphate, and nitrate from roots to shoots of flooded tomato plants Accounting for effects of xylem sap flow rate on concentration and delivery Plant Physiol 107, 377- 384 Epron D, Dreyer...

Ngày tải lên: 08/08/2014, 18:21

... for effects of goserelin incubation on protein expression and phosphorylation Clonogenic cell survival and cell viability were measured after incubation with different goserelin concentrations and ... RNA-expression vs protein expression) Authors' contributions Noticeable was the minor influence of irradiation and the combination of goserelin incubation and irradiation on cell viability One limitation ... incubation on two main MAPK-pathways (phos p38 and phosphor ERK1/2) and on c-fos 24 h after start of incubation, 12 h after irradiation (to reflect long term effects) In this setting, we could demonstrate...

Ngày tải lên: 09/08/2014, 10:21

... has high activity in the morning and low activity in the afternoon, and another group has a reversed pattern with the same magnitude of activity, low activity in the morning and high activity ... indicating subjects and columns indicating the mean function (column 1) and effects on the activity due to apnea level g (column 2) In standard matrix notation each row is a vector of 1’s and -1’s indicating ... center and had higher BMI than found in the general population, so we cannot generalize our conclusions of the impact of BMI on circadian activity to the entire population Table Demographic information...

Ngày tải lên: 10/08/2014, 09:20

... al.: Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-a Journal of Experimental & Clinical Cancer Research 2011 30:22 Figure 10 Effects of UTI and ... cells of the tumor microenvironment, consisting primarily of tumor-related macrophages, can secrete TNF-a continuously to promote tumor formation, invasion, and metastasis via activation of protein-1 ... and TAX on the weight and restraining rate of breast tumor xenografts in nude mice Figure Effects of UTI and TAX on IL-8 mRNA levels in MDAMB-231 cells Figure Effects of UTI and TAX on transplanted...

Ngày tải lên: 10/08/2014, 10:21

... initiation of cell apoptosis and consequent activation of ERK pathway and p-ERK-dependent upregulation of uPA expression In addition, reports have shown that pretreatment of cells with other ERK activity ... http://www.jeccr.com/content/30/1/71 Figure Effects of docetaxe and ulinastatin on expression of uPA, uPAR and p-ERK1/2 in mouse exografts Shown are the quantitative results of uPA, uPAR and p-ERK1/2 expression ... docataxel on breast tumor growth and expression of IL6, IL-8, and TNF-a Journal of Experimental & Clinical Cancer Research 2011, 30:22 Sun ZJ, Yu T, Chen JS: Effects of Ulinastatin and Cyclophosphamide...

Ngày tải lên: 10/08/2014, 10:21

... the root of the The objectives of this study were to evaluate the effects of dopamine and epinephrine infusions in hypoxic piglets on systemic, pulmonary, and mesenteric circulations, and on systemic ... perfusion and oxygenation effects of dopamine and epinephrine Am J Respir Crit Care Med 1997, 155:32–37 Barrington KJ, Finer NN, Chan WKY: A blind, randomized comparison of the circulatory effects of ... 12§ Effects of hypoxia and dopamine infusion on systemic and pulmonary vascular resistance indices i, initial (3 average at 30 of infusion at that dose); f, final (3 average at 60 of infusion)...

Ngày tải lên: 12/08/2014, 18:21

... correlation between end-tidal carbon dioxide tension (petCO2) and cardiac output, coronary perfusion pressure (CPP) and cerebral perfusion pressure, restoration of spontaneous circulation (ROSC), and ... Steen S: The effects of epinephrine/norepinephrine on end-tidal carbon dioxide concentration, coro- Page of (page number not for citation purposes) nary perfusion pressure and pulmonary arterial blood ... that an injection of epinephrine during chest compressions decreased petCO2 and pulmonary blood flow and increased CPP (which then slowly decreased), but the effects on petCO2 and pulmonary blood...

Ngày tải lên: 13/08/2014, 03:20

... hematopoiesis, and motion For motion to occur, the connective tissue components (ligaments and tendons) are essential To carry out this function, ligaments connect bone to bone and tendons connect muscle ... springing action of tendons serves many functions, including conservation of energy, amplification of muscle power output, and attenuation of muscle power input [75] First, in birds and moderately ... response to the force Tendon stiffness is dependent upon the location and function of the tendon and may be influenced by tendon length and cross-sectional area (CSA) For example, a shorter tendon...

Ngày tải lên: 24/08/2014, 09:15

... TOSC TRAP ZnSO4 Phospholipase C Phospholipase D NADPH:protochlorophyllide oxidoreductase Photosynthetic photon flux density Photosystem I Photosystem II Photochemical quenching Non-photochemical ... inactivation of PSII and the inhibition of photosynthesis (photoinhibition) ETC hyperreduction can be prevented by either non-photochemical quenching (dissipation of excess excitation energy ... investigation of phenolic compounds, with the final aim of application in a food product or evaluation of its effects on the human system From the initial quantification and identification of phenolic...

Ngày tải lên: 28/11/2015, 13:46

... comparison of standards used in service quality and satisfaction and provided different measures of service quality and satisfaction (Ekinci, Riley, & Chen, 2001; Liljander, 1994) An understanding of ... research challenges in the sense of discovering and investigating the causal relationships among the constructs of push and pull motivation, satisfaction, and destination loyalty The proposed hypothetical ... satisfaction is important to successful destination marketing because it influences 47 the choice of destination, the consumption of products and services, and the decision to return (Kozak & Rimmington,...

Ngày tải lên: 08/08/2016, 16:57

... product of three nth-power terms that describe the effect of the change in the free metal ion activity (Mact), pH of exposure (pHexp) and pH of acclimation (pHaccl) on metal uptake A coefficient of ... of pH of acclimation described 36% of the variation in zinc uptake Considering only the results of the zinc uptake experiments performed at the pH of acclimation describes 64% of the variation ... this is the result of the combined effect of pH on metal speciation (decreasing free metal ion activity with increasing pH) and on the competition between protons and metal ions for the same uptake...

Ngày tải lên: 16/03/2014, 00:07

... with a 5-nm excitation and emission bandwidth, a 0.5-s response time, and a scan speed of 40 nmÆs)1 All samples contained lM peptide or the same concentration of NATA for control experiments, ... biological action of the peptide Finally, the effect of the tryptophanyl substitution at position 16 on the biological activity was confirmed for the native GGN4 Consistent with the results of the C-terminally ... substitution of the aspartic acid at position 16 by tryptophan, showed antimicrobial activity comparable to that of native GGN4 and less hemolytic activity than that of native GGN4 These results are consistent...

Ngày tải lên: 31/03/2014, 09:20