effects of chitin chitosan and their derivatives on human hemostasis

... the plants and kept on ⁄ 10 MS media A and 3¢-UTR regions PCR amplification consisted of lL short-term exposure (2 min) of leaves under a UV lamp of cDNA template in a reaction volume of 50 lL, ... characterization of the suberization-associated anionic peroxidase of potato Plant Physiol 121, 135–146 12 Wojtaszek P, Trethowan J & Bolwell GP (1997) Reconstitution in vitro of the components and conditions ... Schematic organization of the CrPrx gene The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and the regions of the distal and proximal histidines...

Ngày tải lên: 23/03/2014, 09:21

... thio-b-D-galactoside, cells of a L suspension were harvested and lysed by sonication The crude extract was cleared by centrifugation (10 000 g) and separated on Q-Sepharose fast-flow 26/20, gel-filtration on Sephacryl ... after anion exchange on Q-Sepharose, gel filtration with Sephacryl S-100, further anion-exchange chromatography on MonoQ, and finally separation on immobilized metal affinity chromatography (Talon resin) ... specific inhibitor of serine hydrolases [33] Bacterial expression and purification of tomato MJE To obtain unequivocal evidence of the identity of the cloned sequence, MJE cDNA was subcloned into a bacterial...

Ngày tải lên: 16/03/2014, 18:20

... canonical EF-hands [30] with that in the EF-hands in oyster CaLP, reveals a good correlation of Ca2+-binding ligand positions An exception is the Lys residue in domain of CaLP at ligand position ... Structural variations in EF-hand domains of oyster CaLP may contribute significantly to its specific selectivity for substrates and physiological function Comparison of the aminoacid composition of the calcium-binding ... region between the second and the third EF-hand domain, which contributes to the functional characteristics of CaM to bind to various target proteins [31–33], is the most conserved region of the...

Ngày tải lên: 16/03/2014, 23:20

... which reports on the cloning and expression of two AKR genes from D purpurea Both proteins reduce the ketone group of steroid structures but they are not active on the D4-double bond of the steroids ... both strands of the cDNAs and analysed by the DNASTAR program package (Lasergene) and CLUSTALW Plant treatments To determine the effects of several stress conditions on gene expression, D purpurea ... 17a-hydroxiprogesterone or 5b-pregnan-3b-ol-20-one The reaction was initiated by the addition of the protein, and monitored at 25 °C using a Uvicon 930 spectrophotometer (Kontron Instruments, Germany)...

Ngày tải lên: 18/03/2014, 01:20

... proliferation and expression of differentiation characteristics and to stimulate function, including the synthesis and secretion of thyroid hormones The mitogenic pathways elicited by EGF and phorbol ... Exons are positioned on two BACs containing the p76RBE coding sequence Position of the introns in the cDNA are indicated by lines and positions in amino acids Fig Sequence comparison between human ... protein p76RBE on human chromosome 19 (clone CTC263F14 and 461H2) Analysis of 19q genomic sequence revealed that p76RBE consists of 15 exons (Fig 2B) and maps to 19q13.11 between PDCD5 and FLJ110206...

Ngày tải lên: 23/03/2014, 21:20

... life, and for being my unfailing source of strength and help and hope throughout these years and this thesis i Table of Contents TABLE OF CONTENTS Acknowledgements i Table of Contents ii List of ... Accumulation of klhl Tissue Distribution Analysis of klhl in Adult Zebrafish Expression of klhl is Similar in Human, Rat and Zebrafish Ontogenetic Expression of klhl during Somitogenesis 10 Expression ... 1997; Lander et al., 2001), allowing the correlation of mouse models and human disease This also facilitates the positional cloning of disease genes Second, the study of conserved segments among...

Ngày tải lên: 03/10/2015, 20:57

... together Our phylogenetic reconstruction shows, on the one hand, a close relationship of R pachyptila CA sequences with one of the CA D melanogaster sequences and, on the other hand, the other D melanogaster ... the hydrothermal vent environment and, second, the aposymbioticspecific expression condition could be masked by the developmental condition Comparison with western blots and CA activities studies ... hydrogen bond donors to Q92 (position 129, shared by all organisms of Fig with the exception of Riftia and Caenorhabditis sequences where it is replaced by a serine residue), N244 (position 297, conserved)...

Ngày tải lên: 18/02/2014, 16:20

... EC and CY, reactions were initiated by addition of 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lg of GOU For HP and M, reactions were initiated by addition of 0.1 g of HP or M to 1.0 mL of ... incorporation of 18O2 and 18O-labeled water into GG, reactions were initiated by the addition of 20 lL of a Enzymic reaction and analysis of metabolites Reactions were initiated by the addition of 100 ... molecules of H2O (at Ca and Cb positions) and cleavage the b-aryl ether bond In addition, under differing enzyme conditions, although the fluorescent segregation quantity was proportional to the...

Ngày tải lên: 17/03/2014, 03:20

... the dystonin subfamily and the beta-spectrin superfamily Fig Preparation of an anti-ISP36 serum (A) Expression of human His6-ISP36 in E coli and its purification using Ni-agarose beads A1 and A2 ... housing and killing were in accordance with the guidelines of the Ministry of Education, Science, Sports and Culture of Japan for the use of laboratory animals Cell fractionation Subcellular fractions ... amount of material derived from one A260 unit and mL of isolated nuclei ( · 106 nuclei) was defined as unit (1 U) The suspension was treated with DNase I and RNase A (final concentration of 125...

Ngày tải lên: 30/03/2014, 20:20

... small contraction followed by a stronger contraction On average, participants rated that switch activation required very little effort and was not tiring to use The response time of only one participant ... the unfused mechanical activities of motor units The bulk movement of the muscle and asynchronous activation of fibers at the initiation and end of contraction creates a high-amplitude transient ... and activation thresholds, and provided the user with visual and auditory feedback when a muscle contraction was detected On detecting a contraction, the DTR pin on a serial port of the computer...

Ngày tải lên: 19/06/2014, 08:20

... peri-personal space One of the most compelling needs for wearable technology is in the continuous monitoring of the human body, be that for medical monitoring or to inform the operation of a context-aware ... required for the electronic device, including durability, power consumption, and ease of connection into a circuit Metallic components, designed to function in rigid environments, often not satisfy ... durable and washable over an indefinite period of time In a garment-integrated context, washability of components is important to the preservation of normal user patterns of care and maintenance of...

Ngày tải lên: 19/06/2014, 10:20

... untranslated exons, horizontal lines indicate introns, and the arrows indicate the direction of transcription (c) Alignment of protein products of c19orf10 splicing variants Variants a and b seem ... organization, alternative splicing, and protein sequence of c19orf10 (a) The chromosomal localization of the region containing the c19orf10 c19orf10 gene is indicated on the ideogram The area containing ... Expression of c19orf10 in OA synovium (a) Intense staining of the synovial lining layer and perivascular regions of RA (OCT section) tissue.(b) Intense staining of the synovial lining layer and perivascular...

Ngày tải lên: 09/08/2014, 10:20

... UV-C application led to large increases of leaf DCQs whereas no significant effect was observed on CGA [23] On the basis of our data this might be a consequence of the rapid conversion of CGA to ... responsible for the biosynthesis and accumulation of CGA [25] On the other hand, a lowered HCT expression in tobacco [29], Pinus radiata [30] and Medicago sativa [31] changes lignin amount and ... genes of a pathway within a specific genomic region [33,34] The presence of allelic variation at the sequence level in genes of known biochemical functional is useful for candidate Isolation and...

Ngày tải lên: 12/08/2014, 03:20

... peptide CSWGEYDMC had no effect on agglutination Inhibitory effects of peptides and fusion phages on neuraminidase activity Based on the ability of the peptides and fusion phage to inhibit viral attachment, ... viral-induced agglutination of cRBCs by the peptides and fusion phages were monitored Twofold dilutions of untreated or peptide/ phage treated virus were incubated with cRBCs, and agglutination was observed ... result for the fusion phages and cyclic peptides and partial inhibition result at very high concentration of linear peptide (~35% inhibition at 1000 μM) The latter inhibition may be nonspecific due...

Ngày tải lên: 12/08/2014, 04:21

... long time Figure contribution of unequal crossover and retroposition Relative Relative contribution of unequal crossover and retroposition 'TAG/Retro' is the ratio of the cumulative number of ... relative contributions made by unequal crossover and retroposition to duplications in the human and mouse genomes, and estimated the respective duplication rates of the two mechanisms We conducted ... does not rely on the molecular clock model This second method effectively eliminates erroneous detection of old duplicates as young ones and reduces the influence of gene conversion Consequently,...

Ngày tải lên: 14/08/2014, 08:20

... contributes further understanding of cellular responses following cervical hemi-contusion SCI, and the beneficial effects of bpV(pic) and SC transplantation therapies alone and in combination ... myelination once in contact with an axon, promyelinating SCs alter their gene and protein expression patterns including upregulation of Krox20, and downregulating SOX2 and Jun expression (Jessen and ... sent along non-myelinated axons, making the SC and myelination indispensable for rapid communication between neurons and their targets in the PNS The role of SCs in repair of damaged axons Along...

Ngày tải lên: 24/08/2014, 09:14

... may contribute to its effectiveness in vivo, mainly including induction of apoptosis, stimulation of differentiation, degradation of the specific PML/RARα fusion protein, and inhibition of angiogenesis ... Mechanisms of action of ATO in cells without the PML/RARα fusion protein are summarized as below: Inhibition of signal transduction and angiogenesis [Anderson KC et al., 2002] Induction of oxidative ... oxygen species (ROS) and depletion of glutathione [Park MJ et al., 2005] Induction of apoptosis through depolarization of mitochondrial membrane, activation of caspase-9, caspase-3 and PARP [Akao Y...

Ngày tải lên: 11/09/2015, 16:03

... activation and UV-induced polymerization…………………………………… 45 Fig Schematic diagram of ligands conjugation onto PET-PAAc by a 2-step reaction scheme (solid arrows) and quantitative analysis of the conjugated ... applications which involve the generation of functional tissue grafts in vitro and implantation in vivo to replace the functions of corresponding tissues and (II) diagnostic applications, in which constructs ... galactose ligand (d); (B) Conjugation efficiency curve of GRGDS peptide onto PETPAAc; (C) Conjugation efficiency curve of galactose ligand onto PET-PAAc…………53 Fig Hepatocyte attachment onto different...

Ngày tải lên: 12/09/2015, 08:19

... my deepest gratitude and appreciation to my supervisor Associate Professor Fong Kok Yong, Chairman of Division of Medicine, senior consultant, Department of Rheumatology and Immunology in Singapore ... Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden of SLE was consistently increased in non-white population of the USA, Europe, Canada and Australia ... effects of estrogen and its hydroxylation, decreased androgen levels, hyperprolactinemia, and differences in gonadotropin-releasing hormone (GnRH) signaling 13 Introduction 1.1.2.4 Infection and...

Ngày tải lên: 14/09/2015, 12:42