effects of cell free oxygen carriers
Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
Ngày tải lên :
16/03/2014, 10:20
... after conventional expression in insect cells Ligand binding of cell- free endothelin B receptor A Single-particle analysis of CF-produced ETBcHx The quality of CF-expressed and purified ETBcHx was ... affinity to purified ETB in Brij78 micelles, as Ligand binding of cell- free endothelin B receptor indicated by the determined KD of nm, which is even lower than the value of 29 nm previously determined ... bacterial, yeast or insect cells is still complicated and often inefficient [5–11] Continuous exchange cell- free (CECF) expression systems based on Escherichia coli cell extracts have recently...
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Bactericidal effects of low temperature oxygen plasma on bacillus stearothermophilus and staphylococcus aureus
Ngày tải lên :
18/05/2014, 20:29
... activation of carriers The flux of O atoms was × 10 23 m -2 s -1 and the dose of free O atoms which were received by bacteria again exceeded × 10 24 m -2 61 Vuloševi et al: BACTERICIDAL EFFECTS OF LOW-TEMPERATURE ... both, the activation of carriers and for the treatment of bacteria Heat treatment For studying of temperature effects on bacteria degradation the samples (bacteria on substrate carriers) were treated ... damaged; their cell membrane and cytoplasm were strongly eroded and ruptured Cells in plasma completely lost their cell integrity 63 Vuloševi et al: BACTERICIDAL EFFECTS OF LOW-TEMPERATURE OXYGEN PLASMA...
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![Báo cáo hóa học: "Effects of Pin-up Oxygen on [60]Fullerene for Enhanced Antioxidant Activity" pptx](https://media.store123doc.com/images/document/2014_06/22/medium_blh1403374804.jpg)
Báo cáo hóa học: "Effects of Pin-up Oxygen on [60]Fullerene for Enhanced Antioxidant Activity" pptx
Ngày tải lên :
22/06/2014, 01:20
... b-Caroten Bleaching Method Chloroform solutions of 11 lL of b-carotene (1.0 mg/mL), 4.4 lL of linoleic acid (0.1 g/mL) and 22 lL of Tween 40 (0.2 g/mL) were mixed in a quartz cell equipped with a screw-on ... including an excess of free c-CD) and determined the concentration of solution using a UV-vis spectrometer by comparison of the peak absorbance around 360 nm in water to that of pristine C60O in ... C60O2(e) Fig Plausible structure of water-soluble complexes of [60]fullerene monoepoxide C60O and structure of major isomers of C60O2(cis-1 and e) Synthesis of PVP/C60 and its Oxidized Derivatives...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
Ngày tải lên :
11/08/2014, 00:22
... percentages of cells with tissue showing signs of inhibition of HIV-1 infection transmission were evaluated with respect to the positive control The cytopathic effects of the viral infection of MT2 cells ... 18% of cells (Fig 2b) Raft-media has many antibiotics, which, when combined, result in cytotoxicity Inhibition of cell- free and cell- associated HIV-1 viral infection in the presence or absence of ... cytotoxic to a small population of cells, affecting the viability of 20-23% of the cells in the cervical explant, which correlates with low off-target cytotoxic effects (Fig 2) An ideal microbicide...
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Báo cáo y học: " Prognostic value of cell-free plasma DNA in patients with cardiac arrest outside the hospital: an observational cohort study" pdf
Ngày tải lên :
13/08/2014, 20:21
... Page of 11 sample to measure cell- free plasma DNA was taken from the antecubital vein of each patient immediately after return of spontaneous circulation in the emergency room Plasma and cells ... GE/ml with a sensitivity of 63%, specificity of 69%, positive likelihood ratio of 1.75 (95% CI, 1.44 to 2.35) and correct classification rate of 62% The best cutoff value of six-hour lactate in ... computed tomography ratio of 2.41 (95% CI, 2.04 to 3.26) and correct classification rate of 73% Regarding the secondary endpoint of inhospital mortality, the best cut-off value of plasma DNA was 3,485...
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effects of split injection, oxygen enriched air, and heavy egr rate on soot emissions in a diesel engine
Ngày tải lên :
13/11/2014, 06:41
... rates of change of mass fraction of species are calculated simultaneously Table compares the rates of change of mass fraction of species used in this study and used by Lee et al [58] The lack of ... ii List of Figures iii Nomenclature viii Chapter Introduction Chapter Soot Model Chapter Results and Discussion 3.1 General results 25 3.2 Effects of split injection 42 3.3 Effects of oxygen enriched ... 3.4 Effects of heavy EGR, OEA, and split injection 78 Chapter Conclusions 94 References 97 Abstract 106 - i - List of Tables Table Data for carbon 14 Table Rate of change of mass fraction of species...
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Rheological aspect of cell free layer formation in micro blood flow experimental and numerical study
Ngày tải lên :
09/09/2015, 10:14
... Physiological implication of CFL 13 Overview of dissertation 15 CHAPTER II: A COMPARATIVE STUDY OF HISTOGRAM-BASED THRESHOLDING METHOD FOR DETERMINATION OF CELLFREE LAYER WIDTH IN ... study on the suspension of blood, which includes blood rheology, cell mechanics and physiology Blood, a vital fluid of humans, is a concentrated suspension of cells and cell fragments in plasma ... in the circulation The normal shape of RBC consists of a biconcave discoid with a width of ~7.7 μm, thickness of ~2.8 μm, surface area of ~130 μm2 and volume of ~98 μm3 [37] Due to its shape, RBC...
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Báo cáo khóa học: Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis doc
Ngày tải lên :
30/03/2014, 13:20
... cultures of the wild-type and S12 mutants were inoculated into 2· YT medium (16 g of tryptone, 10 g of yeast extract, and g of NaCl per L) The concentration of streptomycin used for cultivation of ... ratio of the incorporation of [3H]Leu to that of [14C]Phe Results Growth characteristics of S12 mutants We isolated 150 colonies of E coli BL21, grown on plates containing various concentrations of ... slightly lower than that of the wild-type Exceptionally, the doubling times of P90Q, K87E and G91D were 1.5–2 times slower than that of the wild-type (data not shown) Cell- free CAT protein synthesis...
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Báo cáo khoa học: Effects of a novel arginine methyltransferase inhibitor on T-helper cell cytokine production pot
Ngày tải lên :
06/03/2014, 11:20
... production of IFN-c by Th1 cells (left panel) or the production of IL-4 by Th2 cells (right panel) (B) Th1 cells or Th2 cells were stimulated with plate-bound anti-CD3 in the presence of dimethylsulfoxide ... ** 20 IFNγ (ng·mL–1) Fig Effects of compound on Th cell function and proliferation (A) Th1 cells or Th2 cells were stimulated with plate-bound anti-CD3 in the presence of dimethylsulfoxide (DMSO), ... inhibited T -cell proliferation (Fig 5C) Thus, the reduced Th -cell cytokine expression following treatment with AMI-1 or compound is not a result of increased cell death or reduced cell numbers...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
Ngày tải lên :
07/03/2014, 12:20
... [15N]-labelling of selected residues was suppressed by the addition of amino acids at natural isotopic abundance [17] In the case of cell- free protein synthesis, however, the costs of the [15N]labelled ... proteins by cell- free synthesis directly of the reaction mixture without prior removal of low-molecular mass compounds [18,21,22] Selective [15N]-labelling NMR resonance assignments and tracking of chemical ... demonstrated for each of the 19 nonproline residues [18] Time and expense can be drastically reduced by use of cell- free systems [11,18,21], opening many avenues for strategic applications of selectively...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
Ngày tải lên :
07/03/2014, 15:20
... vitro effects of CDO alone in a panel of three colorectal cancer cell lines; second, to investigate the effects of combining CDO with etoposide (VP16), 5-fluorouracil (5-FU) or SN38 on cell growth ... the reduction in cell number may have been the result of a general and simultaneous blockade of all three phases of the cell cycle Cell proliferation is reduced The reduction in cell number may ... may have been a result of an inhibition of cellular proliferation Therefore, at the end of each of the culture schedules, cells were pulsed with BrdU for 30 mins The extent of BrdU incorporation...
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Báo cáo khoa học: The influence of cold shock proteins on transcription and translation studied in cell-free model systems pdf
Ngày tải lên :
07/03/2014, 21:20
... fractions free of nucleic acids were concentrated by ultraltration to a concentration of 5.9 mm From L of cell culture, mg of BsCSP could be prepared The molar extinction coefcient of BsCSP was ... western blot with a-Ras Cell- free expression of H-Ras encoded on pET14brasc in h batch reaction under the inuence of different concentrations of TmCSP as indicated Ten microlitres of each reaction ... different CSPs in a cell- free system without regulatory events of a living cell and therefore focussed on direct nucleic acid binding In the light of our results the reduced expression of bulk proteins...
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Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
Ngày tải lên :
16/03/2014, 14:20
... effects on HbA subunits oxygenation S V Lepeshkevich and B M Dzhagarov the different conformational forms of HbA is a key factor in the complete description of the sigmoidal behavior of HbA oxygenation ... molecule The purpose of this study was twofold First, the mutual effects of pH and NaCl on the bimolecular association rate constant of O2 rebinding and the 6110 quantum yield of BR for the a and ... (1999) Photodissociation of oxygenated forms of the native hemoglobin HbA and its isolated a- and b-subunits and kinetics of molecular oxygen rebinding Dokl Biophys (translation of Dokl Akad Nauk)...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
Ngày tải lên :
16/03/2014, 18:20
... 2004 Cell- free synthesis of 15 N-labelled proteins (Eur J Biochem 271) 4089 4090 K Ozawa et al (Eur J Biochem 271) Fig (Continued) Ó FEBS 2004 Ó FEBS 2004 Cell- free synthesis of The cell- free ... effect of transamination is a reduction of the degree of 15N-labelling in the amino acid of interest In the case of [15N]Gly, much of the label appeared to have been trapped in an amide group of ... Optimization of other conditions for in vitro protein synthesis Fig Cell- free synthesis of PpiB under control of phage k promoters Identical volumes of reaction products were loaded into lanes of a 15%...
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Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf
Ngày tải lên :
16/03/2014, 23:20
... formation of disulfide bonds in E coli cell- free protein synthesis systems [15,16] The active fraction of the scFv produced in the E coli cell- free system with chaperones was nearly half of the synthesized ... the crude cell- free protein synthesis solution The present biotinylation technique may be useful for preparation of biotin-labeled proteins and also for nonradioisotope detection of cell- free synthesized ... A bilayer cell- free protein synthesis system for high-throughput screening of gene products FEBS Lett 514, 102–105 Sawasaki, T., Ogasawara, T., Morishita, R & Endo, Y (2002) A cell- free protein...
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Báo cáo " Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng " docx
Ngày tải lên :
28/03/2014, 10:20
... Concentration of macro-element Fig Growth yield of ginseng cell was affected by concentration of macro elements Values are the quotient of the root dry weight after weeks of culture and the cell dry ... polysaccharide by cell cultures of P quinquefolium, J Pro Biochem 33 (1998) 671 [2] J.W Gao, J.M Lee., Effect of oxygen supply on the suspension culture of genetically modified tobacco cells Biotechnol ... proliferation of callus After times of subculture into the solid medium the callus were inoculated into liquid medium (same with above) Stock cell culture and culture condition Suspended cells of P vietnamensis...
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Báo cáo khoa học: The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system ppt
Ngày tải lên :
29/03/2014, 21:20
... efficiency of protein N-myristoylation and the identity of the amino acids at positions 3, and of the consensus motif in plant target proteins with the use of a Cell- free N-myristoylation of plant ... synthesized in the wheat germ cell- free translation system Effect of the combination of amino acids at positions and on protein N-myristoylation in the wheat germ cell- free translation system Ser6 ... previous finding of an inhibitory effect of Pro at position of full-length translation products on MAP function in the wheat germ cell- free translation system [35] We also detected low levels of [35S]Met...
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Báo cáo khoa học: Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism pptx
Ngày tải lên :
30/03/2014, 10:20
... of the bioreactor, the cells were pumped into the mini-bioreactor as a slow rate over a period of h Typically · 108 to · 108 cells were passed through the carriers, and attachment of 85–90% of ... 31 P-NMR of intact cells Following removal of the upper barrel of the magnet, the mini-bioreactor was carefully guided into the probe head for the collection of NMR data with the aid of a custombuilt ... incubated in the presence of [U-13C]glucose with or without a drug for h following a preincubation period of 18 h in the presence or absence of a drug Condition Effects of tolbutamide and glibencamide...
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Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
Ngày tải lên :
30/03/2014, 13:20
... observed 23 mations depending on the type of micelles [32] An extensive fluorescent part of the cell- free produced EmrE-sgGFP analysis of the effects of different membrane mimetic fusion also ... solubility of overproduced IMPs As the addition of those substances might impact the general efficiency of the cell- free reaction, we first tested the production of GFP in the presence of various ... proteins We have been able to demonstrate the cell- free production of at least mgÆmL)1 of reaction mixture of all of our four target proteins In the case of SugE and TehA, the production rates were...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
Ngày tải lên :
30/03/2014, 16:20
... widths of the bundles of FtsZ Fig Effects of TMAO on denaturant-induced perturbation of the far-UV CD spectra of FtsZ FtsZ (7.3 lM) was incubated with M urea in the absence and presence of different ... denaturant-induced loss of secondary structure of FtsZ TMAO (0.8 m) had minimal effects on the secondary structure of native FtsZ (Fig 2A) FtsZ lost 85% of its secondary structure in the presence of m urea ... counteracting effects of two natural osmolytes namely TMAO and monosodium glutamate against the denaturing effects of urea on the bacterial cell division protein, FtsZ TMAO was chosen because of its...
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