effect of pas on oxidative stress

... the effects of dihydrotestosterone (DHT) on ES cells, in the context of oxidative stress, have not been thoroughly evaluated to date. DHT is a biologically active metabolite of the hormone ... increase in the ROS generation and LPO Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H 2 O 2 -induced oxidative stress 251 Fig. 3. Effect of hydrogen peroxide (H 2 O ... inhibition of H 2 O 2 -induced phosporylation of MAPKs proteins may result in the prevention of downstream events such as activation of NF-κB [44]. Therefore, we suggest that the Effect of dihydrotestosterone...

Ngày tải lên: 07/08/2014, 20:23

... addition of 0.03 μM selenium reduced transactivation by Tat to 25% in cells co-trans- fected with 1 μg of the TRR1 expression plasmid. Addi- tion of selenium had no effect on the expression of EBNA1 ... differential effect of oxidative stress has implications for potential therapeutic interventions that target oxidative stress in patients co-infected with both viruses. Conclusions The activity of EBNA1, ... (D) β-mercaptoethanol Transactivation is expressed as a percent of expression under control conditions Chelation of zinc, oxygen tension and oxidative stress. .. time PCR assay J Clin...

Ngày tải lên: 12/08/2014, 04:20

... seconds, followed by sonication (30 seconds) and centrifugation (2500 rpm for at 4°C) Twenty microlitres of the supernatant was added to 120 μl of 0.1 M phosphate buffer, mM EDTA, pH 7.5, containing ... translocation of NF-κB RelA/p65 generation of intracellular ROS Indeed, previous studies have shown that the cytotoxic ability of cigarette smoke [7,22,25-27] is due to a number of chemical components of ... as cell shrinkage, condensation and fragmentation of nuclear material Necrosis on the other hand, is a passive response characterized by cytoplasmic swelling, rapid loss of plasma membrane integrity,...

Ngày tải lên: 12/08/2014, 16:20

... UNIVERSITY OF ECONOMICS HO CHI MINH CITY International School of Business Hong Phuong Nhi THE MODERATING EFFECT OF LOCUS OF CONTROL ON JOB STRESS- JOB SATISFACTION RELATIONSHIP: EVIDENCE ... and locus of control had significantly negative impact on job satisfaction The results also showed the moderating effect of locus of control on the job stress – job satisfaction relationship in ... MASTER OF BUSINESS (Honours) Ho Chi Minh City - Year 2016 UNIVERSITY OF ECONOMICS HO CHI MINH CITY International School of Business Hong Phuong Nhi THE MODERATING EFFECT OF LOCUS OF...

Ngày tải lên: 24/08/2017, 10:43

... Available Online: 10 May 2019 A field experiment was conducted during kharif season of 2017 on loamy sand soil to study the Effect of Fertility Levels and Stress Mitigating Chemicals on productivity of ... Kumawat4 Department of Agronomy, College of Agriculture, Jodhpur, Rajasthan, India Department of Agronomy, SKN College of Agriculture, Jobner, Rajasthan, India Department of Agronomy, RARI (SKNAU, ... conducted at Agronomy farm, S.K.N College of Agriculture, Jobner in Jaipur district of Rajasthan during kharif season of 2017 on loamy sand soil Geographically, Jobner is situated 45 km west of...

Ngày tải lên: 09/01/2020, 16:39

... UNIVERSITY OF ECONOMICS HO CHI MINH CITY International School of Business Hong Phuong Nhi THE MODERATING EFFECT OF LOCUS OF CONTROL ON JOB STRESS- JOB SATISFACTION RELATIONSHIP: EVIDENCE ... relationships among locus of control, perceptions of stress, and performance Journal of Applied Business Research, 13(4), 1-8 Campbell, L K (2000) A comparison of levels of job satisfaction between ... investigation of relationships between Australian teachers' career stages and locus of control Journal of Educational Administration, 46(1), 8-24 Michael, L (2013) A study on occupational stress among...

Ngày tải lên: 15/07/2022, 20:28

... UNIVERSITY OF ECONOMICS HO CHI MINH CITY International School of Business Hong Phuong Nhi THE MODERATING EFFECT OF LOCUS OF CONTROL ON JOB STRESS- JOB SATISFACTION RELATIONSHIP: EVIDENCE ... and locus of control had significantly negative impact on job satisfaction The results also showed the moderating effect of locus of control on the job stress – job satisfaction relationship in ... relationships among locus of control, perceptions of stress, and performance Journal of Applied Business Research, 13(4), 1-8 Campbell, L K (2000) A comparison of levels of job satisfaction between...

Ngày tải lên: 21/10/2022, 23:15

... pGreenGFP 77 Construction of DAO-AS and DAO-S 79 Construction of chimeric genes consisting of GADS, GAD-AS and tGAD 80 Schematic representation of constructs carrying the gene fusions of the GUS coding ... exogenous stimuli 188 Effects of overexpression and downregulation of DAO and GAD RNAs on shoot regeneration in vitro 192 6.1 Introduction 192 6.2 Results 194 6.2.1 Construction of sense and antisense ... he also gives me professional support, help and inspiration i Table of Contents Page Acknowledgements i Table of Contents ii List of Abbreviations x List of Figures xiii List of Tables xvii Summary...

Ngày tải lên: 12/09/2015, 10:19

... copies in the cell A common plasmid cloning vector: pUC lacZ mulitple cloning sites pUC ApR ColE1 origin of replication Lac+, or blue colonies on X-gal in appropriate strains of E coli High copy number ... information becomes available, the uses of PCR expand • With appropriate primers, one can amplify the desired region from even miniscule amounts of DNA • Not limited by the distribution of restriction ... function, expression, structure, etc • Enzymology - place fragment encoding a particular region of a protein in an expression vector • Population genetics - examine polymorphisms in a population...

Ngày tải lên: 13/03/2014, 18:39

... Comparison of the annotation of 6566 cassava sequences with putative one-to-one orthologs and 4313 sequences without Comparison of the annotation of 6566 cassava sequences with putative one-to-one ... and clone id information to select groups of two or more contigs formed by sequence reads of clones with a second read assembled in only one contig Secondly, we performed a BLASTN analysis of the ... indirectly to stress response The comparison of the annotation of sequences with and without RBHs that we made for the detection of stressinduced genes is based on the following assumption: if there...

Ngày tải lên: 12/08/2014, 05:20

... lack of standardized criteria for laboratory diagnostics of chronic C pneumoniae infection as well as contradictory information about distribution of the pathogen throughout of the tissues of human ... Taq polymerase First run of amplification was conducted under cycling conditions consisting of an initial denaturation at 95°C for min, followed by 45 cycles of denaturation at 95°C for 30 sec, ... CpPLD are among promising candidates for immunological diagnostics of C pneumoniae infection (8, 9) However, changed antigenic profile of C pneumoniae during persistent colonization in human...

Ngày tải lên: 26/10/2012, 08:57

... the amount of IPP incorporation into the toluene/hexane extracts was similar to that of the control experiment with WBP alone On the other hand, addition of WBP to the homogenate of BL21(DE3)/pETHRT2 ... the effect of the HRT2 protein on rubber transferase activity in coincubation with WBP The activity increased as the concentration of HRT2 was raised, until the activity reached saturation The effect ... extracted and cloned into pT7Blue T vector One of the cDNA fragments, which contained regions homologous to those of the conserved regions II, III and IV of cis-prenyltransferase was designated...

Ngày tải lên: 07/03/2014, 21:20

... formation, is expressed in the peduncle region [24] Hence, analysis of the regulation of ppod1 expression should shed some more light on the mechanisms of pattern formation in the foot of hydra ... preparation of extracts, 30 mg of lyophilized H vulgaris (500–600 animals) or frozen foot pieces (about 1000) were sonified for · s on ice (Branson Sonifier 250) in a buffer consisting of 20 mM ... exchanger at a salt concentration of less than 100 mM with a yield of about 10% (Fig 3A) For a comparison the interaction of the foot-specific peroxidase with a cation exchanger, Mono S was tested...

Ngày tải lên: 08/03/2014, 10:20

... amount of small subunit to purify each component Therefore, to simplify the procedure of isolation and purification of mitoribosome, we constructed a plasmid containing the gene for Mrp4, one of the ... under native conditions However, their isolation conditions were suited for tagantibody and tag–calmodulin interactions In contrast, we performed affinity purification under the conditions suited ... course of evolution as in the case of an E coli mutant [27] In both yeast and mammals, about a half of the mrps are unique to mitoribosome and only a small fraction of them are reasonably conserved...

Ngày tải lên: 17/03/2014, 10:20

... BCCP contain a large number of charged residues and we observed inhibition of biotinylation at high salt concentrations, we presume that ionic interactions are involved in the formation of the ... apo-BCCPD67 The reaction was initiated by the addition of purified BPL to a final concentration of lM, and incubated at 70 °C for 30 The reaction was terminated by the addition of ice-cold trichloroacetic ... The final yield of apo-BCCPD67 was  5–10 mg per litre of cell culture and  mg per litre of the holo-form Cloning, expression and purification of BCCPD67 K117L mutant Fig Purification of A aeolicus...

Ngày tải lên: 31/03/2014, 07:20

... interaction must be causing the inhibition of nNOS To con®rm this suspicion and to elucidate the general mechanism of inhibition, an analysis was conducted of the kinetics of the nNOS inhibition reaction ... tumour line testing programÕ of the US NCI (Washington) [12] Neuronal nitric oxide synthase inhibition Inhibition of nNOS was measured by monitoring the conversion of [3H]arginine to [3H]citrulline ... peptides of inhibitor groups 1±3, must therefore inhibit the formation of nitric oxide by either blocking one or more of the cofactor sites on nNOS or by some chemical modi®cation reaction with...

Ngày tải lên: 31/03/2014, 15:20

... above-mentioned reasons, cDNA libraries are preferred over mRNA libraries for genetic manipulations Conventional vs Novel Strategies for cDNA Generation The conventional method of the generation of cDNA ... 0.002% of the reverse transcription mixture suffices for the PCR reaction! The addition of too much starting material to the amplification reaction will lead to production of large amounts of nonspecific ... optimal conditions, in a volume of 10 µL Add a 10-µL mixture containing the selected dNTP(s) (e.g., dATP) at a final concentration of 0.4 mM, µL of the restriction buffer used for digestion, 0.5...

Ngày tải lên: 11/04/2014, 01:04

... preparation A 4-fold genome equivalent bovine BAC library containing 105 984 clones was constructed in pBeloBAC11 (Eggen et al., submitted) Clones were pooled in 46 primary superpools of 304 clones ... non-pseudo autosomal region As a result, ZFY itself will serve as a marker of the Y chromosome in our panel 3.3 Isolation of bovine BAC clones For each marker gene, at least one BAC clone was identied ... Hopkins J.A., Sikela J.M., Use of untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implication for an expression map of the genome, Nucleic Acid Res...

Ngày tải lên: 09/08/2014, 18:21