early acting cytokine displaying lentivectors for gene transfer into hcd34 cells fig 1
Bio nanoparticles and bio microfibers for improved gene transfer into glioma cells
Ngày tải lên :
12/09/2015, 09:10
... Vectors 1. 1.2 .1 Polyethylenimine as a Powerful Non-viral Vector 1. 1.2.2 Baculovirus-mediated Gene Transfer 1. 1.2.3 Nanoparticle-mediated Gene Delivery 13 1. 1.3 Introduction ... 3.4 Discussion 10 2 CHAPTER FOUR CONCLUSION 11 5 4 .1 Results and Indications 11 6 4 .1. 1 Generation and Assessment of Bio-nanoparticles 11 6 4 .1. 2 Assembly and Evaluation ... Microfibers 15 1. 1.3 .1 Mechanism of Microfiber Formation 15 1. 1.3.2 Applications of Self-assembled Polyelectrolyte Microfiber 20 1. 2 Objectives of This Study 24 1. 2 .1 Specific...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 5
Ngày tải lên :
17/09/2015, 17:20
... of horizontal gene transfer among prokaryotes is well established, the gene transfer between bacteria and eukaryotic cells is less well elucidated The finding that non-specific DNA transfer from ... efficient mammalian cell gene transfer By identification and characterization of these factors, more information about the horizontal gene transfer between bacteria and mammalian cells could be yielded ... DNA into mammalian cells Nonetheless, the precise mechanism of gene transfer is still unclear It will be necessary to identify the specific endogenous factor(s) of A tumefaciens required for...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 4
Ngày tải lên :
17/09/2015, 17:20
... A6007-CG9 16 S RNA::gfp A6340-CG9 Strains I rb Fold of acidic inductionc IB pH 7.0 IB pH 5.5 + 12 10 11 60 0.96 16 S RNA::gfp - 11 72 10 75 0.92 A6007(pJYH15) aopB::gfp + 60 502 8.40 A6340(pJYH15) aopB::gfp ... A6007-AG6 katA::gfp + 15 4 14 06 9 .13 A6340-AG6 katA::gfp - 13 87 13 28 0.96 A6340-AG6(pLP36) katA::gfp + 13 95 15 05 1. 08 A6340AG6(pTC147) katA::gfp + (+chvI) 326 13 83 4.24 A6340-AG6(pTC2 01) katA::gfp +d ... aopB-gfp katA-gfp 10 11 12 13 14 15 16 17 18 GFP 7.0 5.5 7.0 5.5 7.0 5.5 7.0 5.5 7.0 5.5 _ A 63 _ A 60 + C 7- G + A 63 _ _ -C 40 G A 60 J (p 07 + YH A + 5) 63 pJ 0( _ YH ) 15 _ 7.0 5.5 7.0 5.5...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 3
Ngày tải lên :
17/09/2015, 17:20
... 293 cells Percentage of attachment or invasiona DH5α RCR2 011 LBA4404 AG6b A6340 A6880
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 2
Ngày tải lên :
17/09/2015, 17:20
... containing pTiA6 virB1::Tn3-HoHo1; virB1- Stachel and Nester, 19 86 Mx226 A136 containing pTiA6 virA::Tn3-HoHo1; virA- Stachel and Nester, 19 86 Mx306 A136 containing pTiA6 virD1::Tn3-HoHo1; virD- Stachel ... Stachel and Nester, 19 86 Mx358 A136 containing pTiA6 virE1::Tn3-HoHo1; virE- Stachel and Nester, 19 86 Mx363 A136 containing pTiA6 virG1::Tn3-HoHo1; virG- Stachel and Nester, 19 86 CGI1 Derivative of ... permeabilization for 10 with PBS/0 .1% BSA/0 .1% Triton X -10 0 at RT, the cells were equilibrated by incubation with PBS/2%BSA for 10 at RT and then incubated with phalloidinrhodamine (Molecular Probes, Eugene,...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 1
Ngày tải lên :
17/09/2015, 17:20
... with VirB10 and is genetically required for the stability of VirB4, VirB8, VirB10 and VirB 11 Purified VirB4 (Shirasu et al., 19 94; Dang and Christie, 19 97; Dang et al., 19 99) and VirB 11 (Christie ... region, the 9.5 kb virB operon encodes 11 proteins, VirB1-VirB 11 (Thompson et al., 19 88; Ward et al., 19 88 ; 19 90; Kuldau et al., 19 90; Shirasu et al., 19 90) These proteins are thought to be ... respective Ti plasmids 1. 1 .1. 2 VirC, VirD and VirE, elements necessary for T-DNA processing 1. 1 .1. 2 .1 Roles of VirC, VirD and VirE in T-complex formation Proteins responsible for the production of...
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Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells docx
Ngày tải lên :
10/08/2014, 05:21
... Mol Genet 2002, 27 :11 5 -13 4 Fischer AJ, Stanke JJ, Omar G, Askwith CC, Burry RW: Ultrasoundmediated gene transfer into neuronal cells J Biotechnol 2006, 12 2:393- 411 doi: 10 .11 86 /14 23- 012 7 -17 -44 ... R3327-AT1 is enhanced by focused ultrasound Gene Ther 2000, 7 :15 16 -15 25 16 Washbourne P, McAllister AK: Techniques for gene transfer into neurons Curr Opin Neurobiol 2002, 12 :566-573 17 Bao S, ... energy J Pharm Sci 2002, 91: 1693 -17 01 14 Miller DL, Bao S, Morris JE: Sonoporation of cultured cells in the rotating tube exposure system Ultrasound Med Biol 19 99, 25 :14 3 -14 9 15 Huber PE, Pfisterer...
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Báo cáo khoa hoc:" Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems" pptx
Ngày tải lên :
11/08/2014, 08:20
... ± 0 ,12 0 0 0 0 0 ,13 ± 0,0 31 13 ,1 ± 1, 4 0 ,12 ± 0,042 0,24 ± 0,060 0 ,10 ± 0,037 0,23 ± 0,0 71 0 ,16 ± 0,046 0,20 ± 0,050 0,47 ± 0,08 22,6 ± 3,4 0,23 ± 0,053 0,30 ± 0 ,11 0, 41 ± 0,0 91 0,22 ± 0 ,13 0,56 ... acknowledged for technical assistance and prof H Kalervo Väänänen for scientific advice This work was financially supported by the National Technology Agency of Finland References 10 11 12 13 14 15 16 17 ... Transfection of non-dividing cells may be obtained by activating nuclear uptake by inserting nuclear localization signals into the transgene [13 ,14 ] Adenoviral gene transfer into osteoclasts has been...
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Gene Transfer Approaches for Gynecological Diseases pot
Ngày tải lên :
22/03/2014, 11:20
... Cancer Res 9: 619 – 624 Vanderkwaak, T J., et al (19 99) An advanced generation of adenoviral vectors 16 1 REVIEW ARTICLE 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 ... adenoviruses Int J Cancer 11 1: 698 – 704 11 1 Ural, A U., et al (2000) Gene therapy for endometrial carcinoma with the herpes simplex thymidine kinase gene Gynecol Oncol 76: 305 – 310 11 2 Bauerschmitz, ... ovarian cancer cells that overexpress HER-2/neu Oncogene 11 : 13 83 – 13 88 Hortobagyi, G N., et al (20 01) Cationic liposome-mediated E1A gene transfer to human breast and ovarian cancer cells and its...
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Báo cáo y học: " Evidence for horizontal transfer of a secondary metabolite gene cluster between fungi" potx
Ngày tải lên :
14/08/2014, 08:20
... globosum CHG 012 43 .1 ( #1) 55 10 0 Magnaporthe grisea CYP1 (MGG_08387.5) part A 81 Chaetomium globosum CHG052 81. 1 (#2) 10 0 Aspergillus oryzae 20 219 .m0 010 1 10 0 Aspergillus flavus 19 18.m0 010 0 10 0 56 Aspergillus ... ACE1 cluster #2 Sordariomycetes 12 39 5280 12 40 52 81 12 41 5282 Fusarium oxysporum 15 296 12 610 BC2 12 42 OXR2 CYP2 12 43 12 45 MFS1 ORF3 RAP2 CYP3 CYP4 SYN2 OME1 12 46 5283 5284 5285 5286 5287 0 310 ... 5284 5285 5286 5287 0 310 0 311 0 312 0 313 0 314 15 297 15 298 Fusarium verticillioides Dothideomycetes Part B RAP1 ORFZ OXR1 CYP1 12 37 cluster #1 Khaldi et al R18.3 12 609 12 608 Stagonospora nodorum...
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Báo cáo y học: "DarkHorse: a method for genome-wide prediction of horizontal gene transfer" pptx
Ngày tải lên :
14/08/2014, 17:22
... 4,092 4 ,10 5 4 ,11 2 4 ,11 2 Other bacteria 13 2 11 2 10 3 96 85 74 76 64 58 58 27 24 18 14 12 11 6 Eukaryotes 6 4 4 3 Archaea 0 0 0 0 0 4 ,16 7 4 ,17 6 4 ,17 9 4 ,17 9 4 ,16 5 4 ,16 7 4 ,17 9 4 ,17 9 4 ,17 9 4 ,17 9 Phage ... setting 0% Dictyostelium 2% 5% 10 % 20% 40% 694 8 31 1,096 1, 485 1, 9 01 2,083 2,353 2,236 2, 011 1, 682 1, 347 1, 267 Bacteria 433 4 31 413 377 308 213 Archaea 72 61 50 35 22 11 3,552 3,559 3,570 3,579 3,578 ... 11 5 99 79 55 56 Non-Firmicutes bacteria 458 422 364 300 229 17 0 Archaea 208 19 7 17 2 13 9 89 46 12 11 1, 440 1, 440 1, 4 41 1,4 41 1,442 1, 443 Eukaryotes Total matches Some columns have slightly lower...
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Báo cáo sinh học: " The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events" pot
Ngày tải lên :
14/08/2014, 19:22
... 10 2 (0.224) 2 .14 × 10 3 (0.0 71) 5.58 × 10 3 (0.056) 0.3 mL of VI Vol 3.96 × 10 2 (0 .11 9) 3.77 × 10 3 (0.038) 7.79 × 10 3 (0.023) Number of target cells 0 .1 mL of VI Vol 6.08 × 10 2 (0.0 61) 5 .14 × 10 3 ... to lentivectors- mediated EGFP gene transfer was dependent on the MOI All four cell lines were seeded in 12 well plate at × 10 4 cells/ well 16 18 hrs before trans- Page of 10 (page number not for ... interests 11 12 13 14 15 None declared Authors' contributions BZ performed the use of MOI to predict gene transfer events in the four cell lines; PM performed titration of lentiviral vectors; HJ performed...
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Báo cáo sinh học: "Human cytokine-induced killer cells have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer" docx
Ngày tải lên :
14/08/2014, 19:22
... a mean of 478.5 pg /10 6 cells (range of 10 7.6 10 79.3 pg / 10 6 cells/ 24 h) compared to irrelevantly transfected (containing eGFP) CIK cells ( 31 pg /10 6 cells) (P = 0.05) CIK cells secreting IL-2 ... citation purposes) Genetic Vaccines and Therapy 2004, 2 :12 17 18 19 20 21 22 23 http://www.gvt-journal.com/content/2 /1/ 12 Interactions between dendritic cells and cytokine- induced killer cells lead to ... the generation of tumor specific lymphocytes and complete tumor regression [23] It is reason- This work was supported by H.W & J Hector-Stiftung, Weinheim, Germany References 10 11 12 13 14 15 16 ...
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Báo cáo sinh học: "Comparative analysis of HIV-1-based lentiviral vectors bearing lyssavirus glycoproteins for neuronal gene transfer" pdf
Ngày tải lên :
14/08/2014, 19:22
... specificity in brain Gene Ther 2007, 14 :13 30 -13 43 Page of (page number not for citation purposes) Genetic Vaccines and Therapy 2009, 7 :1 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Mitrophanous ... [8 ,15 -17 ] Cell culture Cell lines 293T cells (CRL -11 268), human osteosarcoma (HOS) cells (CRL -15 43) and BHK 21 cells (CCL -10 ) were obtained from the American Type Culture Collection (ATCC) DMEM /10 % ... using lentivirus vector-mediated gene transfer J Gene Med 2008, 10 :11 63 -11 75 Anson DS, Limberis M: An improved beta-galactosidase reporter gene J Biotechnol 2004, 10 8 :17 -30 Reiser J, Harmison G, Kluepfel-Stahl...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 5
Ngày tải lên :
16/09/2015, 17:13
... heterogenicity of vtg gene family and expressions of multiple vtg genes were investigated using a model fish, the zebrafish (Danio rerio) The study of vtg genes provides valuable information for further ... that there should not be substantially more vtg genes in the zebrafish genome 2) vtg3 was mapped to chromosome 11 whereas the other five vtgs (vtg1, vtg2, vtg4, vtg5 and vtg7) were mapped to chromosome ... Chapter General conclusions Based on the findings presented in this thesis, the following general conclusions can be drawn: 1) At least seven distinct vitellogenin genes (vtgs) were...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 4
Ngày tải lên :
16/09/2015, 17:13
... -modified pLys144 0.35 2.5 OD 211 OD343 0.3 3-(2-pyridyldithio)-propionate -modified Vtg 0.25 1. 5 0.2 OD OD280 0 .15 0 .1 0.5 0.05 10 11 12 13 14 15 16 17 18 19 20 10 11 12 13 14 15 16 17 18 19 20 Fractions ... subunit of ~19 0 kDa (Fig 4-3A, lane 6) 15 6 Chapter Receptor-mediated gene transfer A kDa M 19 8 ~500 kDa * 11 3 * 75 48.9 33 .1 B Gradient %B 35 A280 1. 5 1. 0 0.5 10 11 12 13 14 15 16 17 18 19 20 212 2 23 ... 4) 19 .1 ± 3 .1 14.3 ± 1. 3 11 .0 ± 2.0 10 .8 ± 4.6 6.4 ± 0.8 5.3 ± 1. 7 5.2 ± 1. 3 27.9 ± 4.4 23.3 ± 13 .0 DNA (n = 4) 14 .1 ± 4.6 13 .3 ± 0.7 11 .1 ± 7.7 7.3 ± 3.7 7 .1 ± 4.5 1. 5 ± 0.8 2.9 ± 0.3 42.8 ± 13 .0...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 3
Ngày tải lên :
16/09/2015, 17:13
... 10 7 (10 0%) 1. 8 1. 8 x107 (10 0%) 2 .1 x 10 6 (10 0%) 1. 7 x 10 6 (10 0%) 0.8 Intestine 7.2 x 10 7 (5 .14 %) 2.6 x 10 6 (0 .19 %) 4.0 x 10 4 (0.003%) 1. 4 x 10 4 (0.0 01% ) 4.0 x 10 8 (10 . 81% ) 5.8 x 10 6 (0 .16 %) 1. 6 ... x 10 5 (0.004%) 1. 0 x 10 6 (0.03%) 5.6 5 .1 x108 (19 .62%) 8.9 x107 (3.42%) 1. 4 x106 (0.05%) 6.4 x105 (0.02%) 1. 8 x 10 6 (10 %) 1. 2 x 10 5 (0.67%) 2.4 x 10 4 (0 .13 %) 9.4 x 10 3 (0.05%) 4 .1 x 10 6 (12 . 81% ) ... (12 . 81% ) 1. 6 x 10 5 (0.50%) 5.3 x 10 4 (0 .17 %) 1. 7 x 10 5 (0.53%) 2.3 5.0 x106 (27.78%) 1. 9 x106 (10 .56%) 3.0 x105 (1. 67%) 7.7 x104 (0.43%) 1. 0 x 10 5 (4.76%) 2.3 x 10 5 (13 .53%) 2.3 ─ ─ 4.3x106 (10 0%)...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 2
Ngày tải lên :
16/09/2015, 17:13
... 0 011 0 010 0000 010 011 111 00 010 010 100000000 010 110 010 100000 000 010 0 010 000 010 0000000 011 000 010 0000000 011 0 0 011 0 010 0000 010 011 111 00 010 010 100000000 010 110 010 100000 000 010 0 010 000 010 0000000 011 0 010 100000000 011 0 Fig 2 -16 Genome ... vtg2 0 011 0 010 0000 010 011 111 00 010 010 100000000 010 110 010 1000000 00 010 0 010 000 010 0000000 010 000 010 0000000 011 0 Hybrids 73-94 M PN 00 010 00 010 000 010 0000 010 110 010 00000000000000 010 00 010 00 0 010 000000000 010 1000000 011 00000000000 010 010 ... AF 017 250 (AAD 016 15) F 12 36 -13 94 aa jsVtg F 12 15 -13 71 aa mVtgI F 12 53 -14 11 aa mVtgII F F 11 95 -13 53 aa 11 71- 1330 aa hVtgA hVtgB F 12 43 -14 00 aa kVtgI F 12 14 -13 73 aa kVtgII F 13 16 -14 75 aa tVtg1 AB088473...
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Characterization of zebrafish vitellogenin gene family for potential development of receptor mediated gene transfer method 1
Ngày tải lên :
16/09/2015, 17:13
... Chapter General Introduction 1. 1 Oogenesis and vitellogenesis 1. 1 .1 Oogenesis The development of an egg is known as oogenesis, which can be divided into different phases including 1) proliferation ... chain) (Sharrock et al., 19 92; Matsubara et al., 19 99; Fig 1- 1B) Additional proteolytic cleavages were observed either in LVI for lamprey Vtg (Sharrock et al., 19 92) or in LVII for Vtgs of several ... posttranslational modifications and with (before or after uptake into oocytes) or without cleavage when forming the yolk (Wahli, 19 88; Byrne et al., 19 89; Fig 1- 1B) The most apparent difference between...
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Construction of nerve growth factor loop 4 containing polypeptides for facilitated gene transfer to neurons
Ngày tải lên :
16/09/2015, 17:13
... Therapy………………… ….…………………2 1. 1 .1 Background of Gene Therapy……… … …………….………2 1. 1.2 Gene Delivery with Nonviral Vectors…………… …………….6 1. 1.2 .1 The importance of gene delivery vectors……………………6 1. 1.2.2 The viral ... vectors…….………………………………………….7 1. 1.2.3 The nonviral vectors………….…………………………… 10 1. 1.2.4 The barriers to nonviral gene delivery….……… ……… 11 1. 1.2.5 The improvement of nonviral vectors.……….… … …… 24 1. 1.3 Targeted Gene Therapy…………………………………………25 ... Therapy…………………………………………25 1. 1.3 .1. Targeted gene therapy… ………………………………… 25 1. 1.3.2 Approaches to targeted gene therapy.…… …………… 27 1. 1.3.3 Targeting of nonviral vectors……………………………… 29 1. 2 Gene Therapy in...
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