dynamics of the recql4 helicase in cells
Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... nonspecific interactions is supported by the observation that preabsorbing of the CB1 antibody with its corresponding blocking peptide eliminated almost all of the staining of these bands (Fig 3A) The ... amplification of cDNA of these cells shows a single band of the expected molecular size (Fig 2A) We also determined the presence of the FAAH protein by Western blot analysis (Fig 3C) An intense staining ... Dendritic cells, derived from bone marrow stem cells, are the most potent antigen-presenting cells of the immune system They play a central role in the initiation of primary immune response and in the...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... coli in vivo can be explained by their competitive inhibition at the ribosome-binding site [32] Together, the M and C domains of human eRF1, in the absence of the N domain, are able to bind to the ... detail below The C domain of eRF1 interacts with the C domain of eRF3 [15–18], and the binding of both factors is essential for fast kinetics of the termination of translation [1] However, in a simplified ... and dynamics of eRF1 middle domain A B Fig 1H,15N-HSQC spectra of the M domain of human eRF1 The numbering of the residues corresponds to that of the full eRF1 protein (A) The Gly region of the...
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Báo cáo khoa học: Involvement of the V2 receptor in vasopressin-stimulated translocation of placental leucine aminopeptidase/oxytocinase in renal cells pdf
... These results indicate that the binding of the hormone to V2 receptors on the basolateral membrane of principal cells stimulates cAMP synthesis, which leads to the translocation of AQP-2 to the ... understand the role of P-LAP in the kidney, further studies are required to determine the actual movement of the vesicles containing P-LAP employing more distinctive renal cells such as the primary ... detected in the distal tubules of the cortex and the collecting ducts of the cortex and medulla (data not shown) Vesicular distribution of P-LAP protein in NRK52E cells The subcellular localization of...
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Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt
... [115] reported the finding of two potential binding sites for HIF-1 in the first intron of the PK-M gene On the other hand, Yamada and Noguchi [116] reported that there is no HIF-1 binding sequence ... melanoma cells, elevation of the cellular Ca2+ concentration leads to detachment of PFK from the cytoskeleton and thus diminishes the provision of local ATP in the vicinity of the cytoskeleton [71] The ... be induced by incubating cancer cells with oligomycin, an inhibitor of mitochondrial ATP synthetase [159,160] Similarly, reduction of OXPHOS by targeted disruption of frataxin, a protein involved...
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Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt
... Discussion The aim of the present study was to investigate the intracellular fate of PrPC by examining for the first time its biosynthesis in the secretory pathway of neuroendocrine cells in vivo and the ... panel), suggesting that the level of GFP–PrPC transgene expression was dependent on the colour of the background of the animal The fusion protein was found only in the NIL and not in the AL of black- ... granules in skin melanophores resulting in darkening of the skin [23] POMC is the major cargo protein in this cell type and during adaptation to a black background the amount of POMC mRNA is induced...
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Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc
... transfection of PC12 cells was performed using LipofectAMINETM (Life Technologies Inc.) according to the manufacturer’s protocol 293T cells were maintained in minimum Eagle’s medium (MEM) containing 10% ... represents the limiting component of TGF-b1 signaling in PC12 cells Smad2 phosphorylation was analysed in parental PC12 cells and in stable PC12 cells expressing TbRII-wt under the control of a doxycyclineinducible ... to TGF-b1 (Fig 2B), again indicating that TbRII is the limiting component of TGF-b1 signaling in PC12 cells The mechanism of Smad reporter activation was investigated by using luciferase constructs...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
... domain ERM contains two DNA binding inhibitory domains flanking the Ets domain: the central 87 amino acid inhibitory domain is poorly conserved and virtually specific to ERM, and the C-terminal ... marks the position of supershifted complexes (B) The binding of in vitro translated ERM is eliminated by mutations in Ets motifs The binding of in vitro translated ERM over the PS1 promoter was ... complexes with in vitro translated ERM which could be supershifted by the antibody, indicating the presence of ERM binding site(s) in each of the three probes We also examined the pattern of complexes...
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báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot
... 2-D pattern of the silver stained gel image of the master gel image 2-D pattern of the silver stained gel image of the master gel image 2-D pattern of the silver stained gel image of a master ... electrophoresis, the gels were fixed in 50% ethanol, and 10% acetic acid for 12 hours Silver staining Proteins were visualized using the silver staining method employing a modification of the method of Heukeshoven ... proteins in the AML samples 23 Spots of the gastric cancer cell line were not represented in the AML samples Intra-individual analysis of the spot patterns showed a high correlation between the...
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Báo cáo y học: "Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis" potx
... to the generation of fibroblast-like cells There was a variation in the expression of NFκB1 mRNA among the RA patients We next examined the relationship of the initial levels of NFκB1 mRNA in ... examine whether the elevation of NFκB1 mRNA expression parallels the elevation of NFκB1 protein expression in bone marrow CD34+ cells The protein expression of NFκB1 was evaluated by staining of permeabilized ... expression of a variety of genes, including MMP-1, under the influence of TNF-α [3] The results in the current study have demonstrated that the expression of mRNA for NFκB1 is increased in RA bone...
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Báo cáo y học: "Regulation of the cd38 promoter in human airway smooth muscle cells by TNF-α and dexamethasone" pot
... binding of AP-1 to the consensus and cd38 putative binding sites in HASM cells TNF-α-induced activation and specific binding of AP-1 to the consensus and cd38 putative binding sites in HASM cells ... mutations in either of the binding sites abolish promoter activation Dexamethasone increases the binding of GR to some of the GRE sites within the promoter and abolishes promoter activation induced ... Dexamethasone induced binding of nuclear proteins to oligonucleotides corresponding to the cd38 putative GRE binding sequences 1, and (labeled GRE1 to GRE4), and decreased binding in the presence of the...
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Báo cáo y học: " Regulation of HIV-1 transcription in cells of the monocyte-macrophage lineage" docx
... mid-frontal gyrus of the brain of infected individuals A high rate of viral replication occurs in this region of the brain Interestingly, the presence of the 6G configuration of this binding site also ... retains the C-terminal DNAbinding domain and competes for binding with the activator isoforms of C/EBP C/EBP isoform expression depends on the differentiation and activation state of cells in the ... by the binding of the DNA-binding domain of p65 to the DNA-binding domain of Sp1 [108] (Fig 4) Sp3 and Sp4 are unable to activate transcription cooperatively with NF-κB [66] In the absence of...
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Báo cáo y học: "Gene function and expression level influence the insertion/fixation dynamics of distinct transposon families in mammalian introns" doc
... smoothing was performed using lowess curves [69] These curves are produced by weighted leastsquare linear fitting within a window sliding through the data The size of the window (span) controls the ... 7:R120 information The initial analysis of the human genome sequence [1] had indicated that the HOX gene cluster is virtually deprived of TEs; the same result was obtained upon analysis of the mouse ... whether any MIR region has been preferentially retained in MIR-rich immune response genes, we retrieved all MIR elements located in the intronic regions of these genes or in their flanking intergenic...
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Effect of changes in dissolved oxygen concentrations on the spatial dynamics of the gulf menhaden fishery in the northern gulf of mexico
... was linked to records of individual purse-seine sets in the Gulf Menhaden fishery We then assessed the spatial effect of DO on four attributes of the commercial fishery: the probability of fishing, ... Locations of total Gulf Menhaden landings (millions of fish) at 5- × 5-min grid cells, summed over all fishing sets in the northern Gulf of Mexico during 2006–2009 (darker shading in cells = more ... each of these regions, beginning with the western region There were significant increases in all response variables as DO concentration declined in the western range of the fishery (Figure 5) In...
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Expression dynamics of the hepatic mitochondrial proteome of the sod2+ mouse in response to troglitazone administration
... defined as having experimental in vitro or in vivo evidence of one or a combination of the following traits: depolarization of the inner transmembrane potential due to either uncoupling or inhibiting ... crossing a threshold at the point -of- no-return • Continued course of disease, in some severe cases despite discontinuation of drug administration, consistent with the concept of accumulation of ... (2D-DIGE), incorporates the labelling of two pools of protein samples and an internal standard with fluorescent cyanine dyes (Cy2, Cy3 and Cy5) permitted the multiplexing of samples in a single 2D...
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Molecular mechanisms underlying the regulation of the positioning and formation of the cleavage furrow in cytokinesis in mammalian cells
... action Inhibit myosin II ATPase activity by binding to myosin II-ADP-Pi Blebbistatin and inhibit the release of the ADP Inhibit actin polymerization by binding to the barb end of the FCytochalasin ... to understand the functions of the different populations of microtubules in mitotic spindle in positioning of the cleavage furrow 1.2.1.1 Roles of microtubules in positioning of the cleavage furrow ... subunits, INCENP (inner centromere protein), survivin and borealin INCENP functions as the scaffold for the whole complex Moreover, binding of aurora B to INCENP stimulates the kinase activity of aurora...
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Spatio temporal dynamics of the urban heat island in singapore 2
... ratio in this report One challenge in determining both ratios is the wide-ranging urban configurations of stations in this study As we are also interested in intra-urban differences and UHI in open ... 4.1 Determining the basis for comparison The main goal of Chapter is to identify and describe distinct patterns of variation in the empirical data collected The definitions used in this Chapter ... these maps to the extent of the study area The script was then used to identify days with heavy cloud cover and rainfall over Singapore These were corroborated using hourly rainfall and wind data...
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Spatio temporal dynamics of the urban heat island in singapore
... UHI in a tropical city Thermal benefits of city parks Temporal dynamics of UHI in Singapore Study of the impact of greenery in an institutional campus in the tropics In uence of land use on UHI in ... spatiotemporal dynamics of the UHI, supported by in- depth statistical analyses of the data collected during the monitoring exercise Beyond describing the data collected in the field, the causal factors ... Despite the importance of the urban thermal environment, limitations in the availability of local data and research efforts mean that gaps remain in the knowledge of the urban thermal environment in...
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Spatio temporal dynamics of the urban heat island in singapore 3
... towards the end of the rainy season are also important to the shape of the UHI curve during these months The difference in cooling rate between urban and rural areas determines the slope of the curve ... values begin to increase across the island A heat island develops in the city area in the south but pockets of warm islands also develop in other parts while the footprint of cool islands shrink At ... across the island Interestingly, the core of the city does not have a distinctive heat island formation, possibly due to the amount of tall buildings providing shade at the street level The largest...
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Spatio temporal dynamics of the urban heat island in singapore 4
... parts of the island At the end of the NE monsoon in March and April, heat islands begin to grow stronger (∼2.5◦ C) and thermal gradients are increasing Values of mean UHIraw continue to increase, ... across the estate to predict heat island intensity in a straight-line function As the H/W ratio used in the present study focuses on the immediate proximity of the sensor, it may be indicative of the ... metadata and the accounting for various confounding factors such as weather and thermal inertia are in- line with the criteria laid out by Stewart (2011) This achieves the initial goal of having a scientifically...
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Spatio temporal dynamics of the urban heat island in singapore 5
... effect in Metro Manila International Journal of Remote Sensing, 29(10):2799–2818 Tso, C (1994) The impact of urban development on the thermal environment of Singapore In WMO, editor, Report of the ... (1997) The vegetation in the nature reserves of Singapore Garden’s Bulletin, Singapore, 47:147–159 Dufresne, J.-L and Bony, S (2008) An assessment of the primary sources of spread of global warming ... (2001) On the impact of urban climate on the energy consumption of buildings Solar Energy, 70(3):201–216 Singapore Meteorological Services (1986) A study of the urban climate of Singapore In Chia,...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25