drug membrane interaction and pharmacokinetics of drugs

... detergent-resistant membrane fractions (DRMs) was defined by both Triton X-100 and Chaps detergents, and found approximately independent of the ligand (IL-2) occupation level of receptors on T cells Analysis of ... Here, a minimum of 5000 pixels of digital cell images were analyzed in terms of bleaching kinetics and the efficiency of FRET was calculated from the mean bleaching time-constants of the donor dye ... from coprecipitation and cocapping Interaction of CD48 and CD25 in the plasma membrane of Kit225 K6 cells as revealed by immuno-coprecipitation CD25 content of the cell lysate was immunoprecipitated...

Ngày tải lên: 17/03/2014, 23:20

... immunodetection system, as described in (B) The densities of the bands were determined and plotted as a function of the amounts of LolB and mLolB LolB and mLolB (each lg) were analysed by SDS–PAGE, followed ... immunoblotting (Fig 4A) OmpA and SecG were examined as markers of the outer and inner membranes, respectively Four outer membrane- specific OM A IM In vitro membrane targeting activity of mLolB OmpA SecG ... amount of added mLolB Moreover, mLolB exhibited no membrane preference and caused incorporation of Lpp into both the outer and inner membranes We then examined the mLolB-dependent localization of...

Ngày tải lên: 23/03/2014, 05:22

... platelet count of 9.2 × 104/μL, PT of 37% (PT-INR 1.78), T-Bil level of 11.7 mg/dL, ALT level of 260 IU/L, and serum albumin level of 2.9 g/dL Serum HGF and AFP levels were 1.07 and 3.9 ng/mL, ... intravenously for 14 days, and urinary excretion of albumin and protein was measured before (day 1), during (days and 14), and and 14 days after HGF administration Repeated doses of rh-HGF induced an ... days in patients and Patients and required cessation of rh-HGF on days 14 and 13, respectively, because of increased serum creatinine (2.1 mg/dL) and oliguria, respectively Both of these symptoms...

Ngày tải lên: 18/06/2014, 19:20

... for the drug (kel = 0.693/t 1/2); t = difference in time between C1 and C0; and t 1/2 is defined as the half-life of the drug Descriptive statistics for the pharmacokinetic parameters of saquinavir-SGC ... population consisted of 110 patients: 52 and 58 patients in the saquinavir-SGC/ritonavir and efavirenz arms, respectively The safety population consisted of 161 randomized patients: 81 and 80 patients ... population of patients treated with either saquinavir-soft gelatin Figure Mean levels of triglycerides and total cholesterol in the safety population of patients treated with either saquinavir-soft...

Ngày tải lên: 20/06/2014, 08:20

... safety, and pharmacokinetics of IV ibuprofen in adult subjects with fevers greater than or equal to 101.0°F Subjects were randomized to receive a placebo or one of three doses of IV ibuprofen (100 ... 200 mg and 400 mg dose levels of IV Ibuprofen, see Table Figure demonstrates the mean plasma concentrations of ibuprofen for each of the three drug dosage arms in both the critically ill and non-critically ... ibuprofen group (primary endpoint) showed 24 of 31 (77%) of subjects, compared to of 28 (32%) of the placebo group with a temperature

Ngày tải lên: 13/08/2014, 21:20

... analysis of the Raman spectroscopy and characterization of single-walled CNTs The ratio of G-band to D-band intensities can be used as an indicator for the purity of CNTs The lower the D-band compared ... discovery of single-walled CNTs [17,18], etching [19] and filling [20] of CNTs, magnetic measurements [21,22], purification of CNTs [23] and etc Recent developments and potential applications of CNTs ... electronic band structure of the CNT which is in turn dependent on the particular diameter and chirality PL thus serves as a direct optical probe of the electronic band structure and structure of single-walled...

Ngày tải lên: 11/09/2015, 10:05

... INTERACTION AND ORGANIZATION OF DNA IN CONDENSED PHASES DAI LIANG (Ph.D.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTER OF PHILOSOPHY DEPARTMENT OF PHYSICS NATIONAL UNIVERSITY OF SINGAPORE ... molecular details of DNA molecules are sometimes essential for the understanding of a variety of experimental observations vii LIST OF TABLES Table 2.1 X-ray and neutron scattering lengths of some elements ... understanding of DNA interactions and organization in dense phases at the atomic scale Furthermore, it will be shown that molecular details of the DNA molecules are essential for the understanding of...

Ngày tải lên: 14/09/2015, 14:13

... review of information provided to individual recipients of IND drugs, medical monitoring and reporting of the use of such drugs, information about shipment and storage of drugs, disposition of unused ... development or acquisition of drugs and biologics for CBW defense It also recommends organizational changes in the Office of the Secretary of iii iv The Acquisition of Drugs and Biologics for CBW ... (2001), p A24; and Fialka et al (2001) The Acquisition of Drugs and Biologics for CBW Defense cals (drugs) , and the protection of personnel by immunization, mainly by vaccines The objective of the U.S...

Ngày tải lên: 17/02/2014, 11:20

... attributed to bH of Ala12 and NH of Val15, and aH of Pro13 and NH of Val15 Ó FEBS 2004 Structural study of internal fusion peptide of ASLV (Eur J Biochem 271) 4733 Conformational alteration of IFP–wt ... and internal FPs, in the hope of improving our understanding of the role of fusion peptides in the fusion event Structure and membrane interaction of IFP–wt Binding of IFP–wt to lipid vesicles ... self-assembly of the envelope glycoprotein of ASLV bound to membrane Effects of pH on the secondary structure and membrane interaction of IFP–wt Based on studies on the molecular clustering of EnvA...

Ngày tải lên: 19/02/2014, 16:20

... studies of the interaction of antimicrobial peptide and lipid bilayers Biochem Biophys Acta 1462, 185–200 Epand, R.M & Vogel, H.J (1999) Diversity of antimicrobial peptides and their mechanism of ... three-dimensional structures of both peptides in membrane models Further insight into the membrane- binding properties of the peptides and their depth of penetration into the lipid membrane has been obtained ... illuminates the interactions of the peptides with lipid membranes The colorimetric assay was shown to correlate blue-red transitions of lipid/PDA vesicles with the degree of membrane penetration and lipid...

Ngày tải lên: 17/03/2014, 11:20

... temperature (Figs and 6) Control experiments recorded the Tc of both Myr2-PGro and Myr2PCho membranes as 25 °C and that of Myr2-PEtn membranes as 47 °C (Figs 3A–C and 6A)C) In the presence of P4, no ... of the peptide (Fig 3B) The presence of P5 had a strong effect on the Tc and membrane fluidity of both Myr2-PCho and Myr2-PEtn membranes with Tc being recorded as 13 and 42 °C, respectively, and ... the membrane fluidity of the membrane was detected (A) The Tc of Myr2PEtn membranes alone was recorded as 47 °C (j) and although in the presence of P4 (d), no significant effect on the fluidity of...

Ngày tải lên: 31/03/2014, 08:20

... standard parameter value for a patient with the standard BW value and the power parameter estimate for BW, and Pi and BWi are the parameter and BW of the ith individual Graphical evaluation of ... [15], ICU length of stay before inclusion and cause of septic shock The volume of fluid administered for resuscitation of shock before C1 was recorded Invasive blood pressure and heart rate were ... absence of any measurement of the production of reactive oxygen species and oxidized catecholamine metabolites, we cannot exclude a small influence of oxidative stress on epinephrine pharmacokinetics...

Ngày tải lên: 13/08/2014, 18:22

... method causes matting of the mucosae and Schneiderian membrane and makes elevation of the sinus membrane without disruption impossible Clinical Significance The principal advantage of the technique ... characteristics of OAC/OAF; the apexes of tooth 26 were in extremely close approximation to the maxillary sinus, and an area of periapical rarefaction was evident (Fig 1) After the failure of the endodontic ... sinus over the involved tooth and the localized swelling and thickening of the sinus mucosa; only close the root of 2.7 a periapical lesion was present; radiopacity of different degrees was evident...

Ngày tải lên: 25/10/2012, 11:48

... differentiate the administrative route of drugs and their teratogenic potential We should therefore evaluate the use of the same drugs (e.g., corticosteroids and antifungal agents) according to ... eye and ear) and inhaled aerosol treatments separately Finally it is necessary to differentiate drugs and pregnancy supplements within medicinal products Drugs are used for the treatment of maternal ... factors and when asked about the history of their pregnancy, give a long list of supposed agents On the other hand the mothers of healthy babies are thinking of the present and future of their...

Ngày tải lên: 02/11/2012, 11:12

... polymerization of styrene and the blending of PS and SPS polymers have affected the surface structure of the PVDF membrane obviously The cross-section morphologies of the PVDF membrane, the PVDF-PS membrane ... PVDF-SPS membrane and Nafion-117 membrane were measured Figure shows that the two kind of membranes report a linear relationship between CB and t, and the methanol permeability of the membranes ... PVDF-SPS membrane: (a) C1s, (b) O1s, (c) S2p 3.4 Morphology of the PVDF-SPS composite membrane The morphologies of the pristine PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane were...

Ngày tải lên: 05/09/2013, 16:11

... Color Atlas of Pharmacology © 2000 Thieme All rights reserved Usage subject to terms and conditions of license 62 Drug- Receptor Interaction Enantioselectivity of Drug Action Many drugs are racemates, ... entry of Na+ (and exit of some K+), membrane depolarization, and triggering of an action potential (p 82) The ganglionic N-cholinoceptors apparently consist only of ! and " subunits (!2"2) Some of ... Lüllmann, Color Atlas of Pharmacology © 2000 Thieme All rights reserved Usage subject to terms and conditions of license 66 Drug- Receptor Interaction Mode of Operation of G-ProteinCoupled Receptors...

Ngày tải lên: 26/01/2014, 19:20

... series of GST-fused deletions of the CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the ... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned ... functional importance of the v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neurons and cerebellar granule cells Further studies of these newly-identified...

Ngày tải lên: 14/02/2014, 19:20

... maximum of the a band at 557 nm absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b Heme was extracted from the protein with acidic acetone and ... behavior is typical of integral membrane proteins The polypeptide with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1) Identification of the genes ... out to be a major membrane protein and contained most of the heme present in the membrane fraction Hdr from M barkeri is composed of only two subunits, a b-type cytochrome and the hydrophilic...

Ngày tải lên: 21/02/2014, 03:20

... between the N- and C-terminal halves of the OPA, AAT, and DAACS families and the N- and C-terminal halves of ST3 (open bars), between the N-terminal halves of ST3 and the C-terminal halves of ST3 (grey ... (top) and analysis of the membrane topology of the two domains (bottom) Top: the left panel shows the hydropathy profiles of the N-terminal half (blue) of REUT3304rmet of R metallidurans and the ... the N- and C-terminal halves of these unrelated families is very low and only very few of the hits have E-values in the range of pE ‡ (Table 2) Importantly, the percentage of observed hits and their...

Ngày tải lên: 07/03/2014, 17:20