drug drug interactions an in vitro approach

... as Fe and Cu [32-34]; other groups emphasize that organic and black carbon components found mainly in ultra-fine particles confer greater in vivo and in vitro toxicity than fine particles, and ... (ROS) induce chemical and structural changes on human insulin in vitro, including alterations in its immunoreactivity Frontiers in bioscience 2005, 10:838-843 Olivares-Corichi IM, Ceballos G, Medina-Santillán ... concentration [25] An important factor for the mechanisms involved in cells death an injury, is the production of free radicals Experimental and clinical data suggest that oxidants play a role in the pathogenesis...

Ngày tải lên: 20/06/2014, 00:20

... in combination against clinical strains of extreme drug- resistant Acinetobacter baumannii to all antibiotics including polymyxin B in Singapore” 20th European Conference on Microbiology and Infectious ... “Pharmacokinetic/pharmacodynamic modelling of polymyxin B, rifampicin and tigecycline against pandrug-resistant Acinetobacter baumannii in an in- vitro model” 20th European Conference on Microbiology and ... Tan TY, Lee W, Sasikala S, Tan TT, Hsu LY, Kwa AL In- vitro activity of polymyxin B, rifampicin, tigecycline alone and in combination against carbapenem-resistant Acinetobacter baumannii in Singapore...

Ngày tải lên: 10/09/2015, 09:22

... medicinal plants For instance, morphine [10] was first isolated from opium extract in 1815 and papaverin [11] in 1848 Another prominent example is the discovery of penicillin [12] as an antibiotic ... targets mainly by non-covalent bonds such as van der Waals interactions, the same hydrogen bond interactions, and electrostatic interactions [14] Only in few instances are covalent interactions ... querying, updating and re-structuring of the database The relational approach is so far the most widely utilized and dominant mainstream approach to data management In the manual approach, the information...

Ngày tải lên: 15/09/2015, 22:24

... membrane[36] and also the loading procedure does not affect the MCV This finding is in agreement with previous report[34] Non-significant change in both MCH and MCHC can be explained by pravastatin ... Pharmacokinetic-pharmacodynamic drug interactions with HMG-CoA reductase inhibitors Clin Pharmacokinet 2002; 41(5):343-370 Hatanaka T Clinical pharmacokinetics of pravastatin: mechanisms of pharmacokinetic ... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence...

Ngày tải lên: 25/10/2012, 11:10

... Thus far, the obvious approaches of expressing and purifying I7L from prokaryotic and eukaryotic expression vectors and combining with peptides or proteins containing a canonical A-G-X cleavage ... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr ... of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64...

Ngày tải lên: 19/06/2014, 08:20

... longitudinal-circumferential shear modulus G, with published machine testing determined values ranging from 3300 to 5000 (N/mm2) for human and bovine femur and tibia [17] Thus use of E = 18,000 and ... ostectomized-instrumented equine radii It is the authors' opinion that the 3D loading-measuring system described in this manuscript is capable of creating "worst case" clinically relevant loading conditions, ... lateral and cranial (anterior) radius surfaces with at least a 0.5 cm distance from potting material, and to have sufficient bone-potting interface to transmit loads to failure of the instrumented...

Ngày tải lên: 20/06/2014, 01:20

... type II and aggrecan core protein was steadily expressed in fibrin/PLGA and PLGA Interestingly, suppression of collagen type I was observed in fibrin/PLGA and PLGA at weeks and weeks β-actin gene ... implantation in a canine model: change in composition of reparative tissue with time J Orthop Res 2001, 19:482-492 Munirah S, Samsudin OC, Chen HC, Hussein SSS, Mohd Azam Khan GK, Aminuddin BS, ... Cambrex Bio Science Rockland, Inc USA), stained with SYBR® green nucleic acid gel stain (Cambrex Bio Science Rockland, Inc USA) and visualized by UV transillumination using gel documentation system...

Ngày tải lên: 20/06/2014, 01:20

... increasing the action of the first and negating that of the second That drugs can interact is not unexpected Systematically investigating such interactions in vitro is slowly becoming a routine ... Scotland) to remove cells remaining in the intra-trabecular spaces and plated at fragment per well in a 24-well plate containing ml of control medium (α-MEM containing 1% Gentamicin v/v and 10% ... guidelines has been established in some reviews[17,18] In light of these many uncertainties, we carried out an invitro study of the effect of various antirheumatoid drugs alone and in combination...

Ngày tải lên: 20/06/2014, 01:20

... Thus far, the obvious approaches of expressing and purifying I7L from prokaryotic and eukaryotic expression vectors and combining with peptides or proteins containing a canonical A-G-X cleavage ... Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for hr ... of inhibitors on in vitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64...

Ngày tải lên: 20/06/2014, 04:20

... than group I In TS, the usage of the MPS system has some disadvantage such as loosening, bending, breaking or pulling out of the screws and plates, and the maximum inter- segmental rigidity and ... Harper KD, Nightingale RW The role of imaging and In Situ biomechanical testing in assessing pedicle screw pull-out strength Spine 1996, 21, 1962-1968 Sandén B, Olerud C, Petrén-Mallmin M, Larsson ... calculated in Fig Calculation of radian angles for converting the forces to the moments Fig The results of the Euclidian distance method and the four main clusters two steps: First, the stiffness in...

Ngày tải lên: 07/08/2014, 20:23

... immunofluorescence and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected ... tissue In the present article we established an in vitro model system to investigate the survival and phenotype of human MSCs following injection into bovine NP tissue explants to test the following ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer...

Ngày tải lên: 09/08/2014, 01:22

... quiescence, and induces apoptosis in HT-29 colon adenocarcinoma cells J Clin Invest 1995, 96:491-503 47 Zhang X, Morham SG, Langenbach R, Young DA: Malignant transformation and antineoplastic ... important impact on long-term out- come Therefore it remains important to extend these in vitro studies with animal in vivo studies and even clinical setups Conclusion Although they are in vitro findings, ... selective COX-1 inhibitor SC-560 had effects similar to those of indomethacin, indicating that inhibition of COX-1 results in an adverse effect on proteoglycan synthesis and retention In contrast,...

Ngày tải lên: 09/08/2014, 07:20

... mean ± strandard error * P < 0.05 2AP, 2-aminopurine; cpm, counts/ min; PKR, protein kinase R; sGAG, sulphated glycosaminoglycan tive mRNA splicing, namely types IIA and IIB, which include and ... collagen-glycosaminoglycan interactions J Biol Chem 2001, 276:5303-5309 Chua KH, Aminuddin BS, Fuzina NH, Ruszymah BH: Insulintransferrin-selenium prevent human chondrocyte dedifferentiation and promote ... This in accordance with studies in human keratinocytes, which have shown that a rapid (15 minutes) but transient (returning to baseline by hour) increase in endogenous ceramide occurs following...

Ngày tải lên: 09/08/2014, 08:22

... activity, including phagocytosis, respiratory burst, and transendothelial and transepithelial chemotaxis induced by arginine-glycine-aspartic acid (RGD) containing synthetic peptides and protein [17] ... of α5β1 integrin and CD47/IAP [41] It has been speculated that the five membrane-spanning segments of CD47/IAP and the two membrane-spanning domains of the heterodimeric integrin form an ad hoc ... assessed by measuring either changes in membrane potential, changes in protein tyrosine phosphorylation, or changes in the relative levels of aggrecan mRNA within cells Available online http://arthritis-research.com/content/10/1/R4...

Ngày tải lên: 09/08/2014, 10:22

... several genes involved in the NF-κB signaling pathway showed differential expression, including interleukin-1 receptor antagonist (IL1RN), receptor-interacting serine/threonine kinase (RIPK2), ... TH and JR participated in gene expression data processing and in study design and coordination CK and MS conceived the study and participated in its design and coordination All authors read and ... categorized into six rheumatoid arthritis-relevant groups and are listed with accession number, mean fold change in expression and mean signal intensity (generated by GCOS and RMA) Annotation of mean...

Ngày tải lên: 09/08/2014, 10:22

... doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other LPS-responsive cells such as neutrophils, is known to be initiated by interaction ... can depend on the type and intensity of the stimulus, and vary according to the considered cytokine LPS induced similar levels of IL1 production in males and females, with levels increasing in ... syndrome group had a significantly lower median production than females (p < 0.03) Increasing the concentration of LPS increased the median production of IL1 in females and in males (p < 0.036; p

Ngày tải lên: 11/08/2014, 03:20

... doses of LPS and Pokeweed Mitogen lectin as stimulants in vitro LPS-induced signalling in macrophages, and in other LPS-responsive cells such as neutrophils, is known to be initiated by interaction ... can depend on the type and intensity of the stimulus, and vary according to the considered cytokine LPS induced similar levels of IL1 production in males and females, with levels increasing in ... syndrome group had a significantly lower median production than females (p < 0.03) Increasing the concentration of LPS increased the median production of IL1 in females and in males (p < 0.036; p

Ngày tải lên: 11/08/2014, 06:22

... immunoprophylaxis in patients with neoplastic deseases II Estimation of antihaemagglutinin and antineuraminidase antibody titre or influenza viruses A and B Int Rev Allergol Clin Immunol 1999, ... viruses were produced and inactivated with an UV-GS linker The virus titre inducing 20-30% IFN synthesis (calculated with an IFN-a standard) was determined in serial dilutions and kinetic experiments ... to induce type I IFN in primary human cells [22,23] In particular, IFN-a production can be induced in human Page of mononuclear cells in the presence of purified, recombinant HSV-1 glycoprotein...

Ngày tải lên: 11/08/2014, 08:21

... modulating their expression or intrinsic adhesive functions [18] PMNs maintain several of these receptors in an inactive state during transit in the bloodstream and extracellular fluids, and only ... Martini Hospital, Turin, for providing human umbilical cords, and the Blood Bank, Molinette Hospital, Turin, for providing human blood References 10 11 12 13 14 15 Vane JR, Bakhle YS, Botting ... adhesiveness induced by complement fragments C5a and C5a des arg and formyl-methionyl-leucyl-phenylalanine in vitro J Clin Invest 1984, 74:1581-1592 Zimmerman GA, McIntire TM, Prescott SM: Thrombin stimulates...

Ngày tải lên: 11/08/2014, 08:21

... no competing interests SC declare no competing interests LB declare no competing interests SR declare no competing interests MM declare no competing interests EC declare no competing interests ... conceived and designed the study, coordination and manuscript preparation MB was involved in isolated human bronchial ring experiments and helped in manuscript preparation SC participated in the ... experiments, was involved in HASMC culture, performed in vitro cAMP studies and helped in the manuscript preparation LB participated in the design of the experiments and was involved in isolated human bronchial...

Ngày tải lên: 12/08/2014, 16:20