drug device development and application for approval

... ST+ drugs and 239 ST- drugs, 103 TdP+ drugs and 157 TdP- drugs were retained For study of serious psychiatric ADRs, 321 and 169 drugs were identified PADR- and PADR- respectively All the information ... desirable to facilitate drug development and safety assessment 1.2 QSAR studies for ADR and toxicity prediction To deliver promising drug candidates to reach the late stage of drug development with ... 83 6.3.1 Base and ensemble model performances for SJS/TEN study 83 6.3.2 Base and ensemble model performances for TdP study 84 iii 6.3.3 Base and ensemble model performances for serious psychiatric...

Ngày tải lên: 10/09/2015, 09:02

... required upon hand-off for assay validation SJ, AW, SWA and KA performed the in vitro assays on monkeys treated with Ab-01 and control Ig and analyzed the data, and KA and SA performed the experiments ... ABI, and are described in Table cDNA synthesis, preparation of samples for TLDA assay and measurements of RNA concentration were performed as described for the human assay Results Time course and ... quantification) values of untreated control and rhIL21-treated samples for each of the donors, and subsequently the mean and standard deviation were determined for each gene shown A: Percent inhibition...

Ngày tải lên: 18/06/2014, 16:20

... and probes for SCO4410, SCO5639, SCO3202 and SCO4157 were designed using Primer software and used for qPCR as described previously [7] The sequences for the forward and reverse primers and dual ... open software development for computational biology and bioinformatics Genome Biol 2004, 5:R80 R [http://www.R-project.org] R Development Core Team: R: a Language and Environment for Statistical ... probe set were picked from probes performing above average with a strong bias for very well performing probes For both array formats the probes were deposited at random positions on the slide surfaces...

Ngày tải lên: 14/08/2014, 21:20

Ngày tải lên: 14/11/2014, 12:55

... anti-Ki-67 (DAKO) for h at room temperature, and incubated with secondary antibodies before tissues were further probed for cH2AX overnight at °C Cryosections (10 lm) were fixed in % PFA for 10 and stained ... have distinct bands detectable by myImageAnalysis In this case, the selected band widths were the same for every lane in each blot Band intensity (volume of band) was quantified and normalized ... the potential for influenza to induce DNA strand breaks, we performed a comet assay, a method that is well established for directly measuring physical DNA single stranded lesions and DSBs [23,...

Ngày tải lên: 09/09/2015, 08:16

... DEVELOPMENT AND APPLICATION OF A HIGHTHROUGHPUT SCREENING ASSAY FOR DISCOVERY OF STARCH HYDROLASE INHIBITORS LIU TING TING (BSc, MSc Wageningen University) A THESIS SUBMITTED FOR THE ... effort to identify novel drugs that can manage type diabetes more efficiently Plantbased products have been popular all over the world for the centuries, and many of them have been studied and ... plants, and the bioactive ingredients derived from plants, have been characterized in vitro or in vivo for their role in preventing the type diabetes Edible plants are therefore good candidates for...

Ngày tải lên: 10/09/2015, 09:01

... profiles Information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species A comprehensive database to understand the ... Rong and Ms Jia Jia I thank them for their valuable support and encouragement in my work Finally, I owe my gratitude to my parents, husband and daughter for their love, constant support, understanding ... related to disease initiation and progression and can provide candidate disease targets The interaction between disease targets and therapeutic molecules is crucial for drug discovery Therapeutic...

Ngày tải lên: 12/09/2015, 08:20

... droplet-based LPME 1.1.2.1.2 Developments and applications of droplet-based LPME 1.1.2.2 Membrane-based LPME 1.1.2.2.1 Theories of membrane-based LPME 13 13 1.1.2.2.2 Developments and applications of membrane-based ... solvents, much work has been devoted to the development and application of miniaturized or microscale LLE during the last 20 years In 1986, an initial effort to perform solvent-based microextraction was ... pushed and pulled, the organic solvent film was formed in the microsyringe barrel and used as the extraction interface This method was shown to be a fast and simple extraction method for volatile...

Ngày tải lên: 12/09/2015, 08:20

... Chao Fu and Dr Tapabrata Ray, for their support and encouragement I would like to express my deepest gratitude to my parents, Varadarajulu and Nagama Devi, and my siblings, Naren and Meera, for their ... essential for understanding the physical behaviour and characterising the performance of complex radio frequency (RF) and microwave systems Efficient computational electromagnetics (CEM) techniques and ... multiplication operations of the form M −1 z is simply performed by forward and backward substitutions using R An efficient preconditioner is one for which κ(M−1 A) is bounded and independent of the mesh...

Ngày tải lên: 14/09/2015, 11:39

... E and Miss Fang G H I sincerely appreciate the National University of Singapore for providing me the financial support during my research Finally, a million thanks to my parents and husband for ... techniques to improve CE performance, and applications of CE as well A novel potential gradient detector (PGD) was designed and coupled with a portable CE system for separation of DNA fragments ... cost effectiveness and miniaturization, and therefore has great potential for on-site analysis of various pollutants at trace level CE is found to be a versatile analytical tool for the analysis...

Ngày tải lên: 14/09/2015, 11:40

... biological systems has been an important tool for the exploration of cellular processes (Kelleher, 2004; Wiechert and Noh, 2005) and drug development (Perkins and Frier, 2004) In this post-genomic era, ... large-scale analysis of proteins, i.e proteomics (Pandey and Mann, 2000; Tyers and Mann, 2003) Therefore, the advancement of proteomics relies largely on the development of state-of-the-art proteomics ... our understanding of protein activity and function In the post-genomic era, proteomic studies complement the information acquired from genomics and functional genomics, thereby expanding our...

Ngày tải lên: 16/09/2015, 08:30

... Facilitating Drug Discovery 1.4.1 Overview of emerging technologies of drug discovery 1.4.2 The need of drug target databases for drug discovery 1.4.3 Adverse drug reaction (ADR) target database for drug ... academic and company scientists And because of the efforts of these scientists and funding agencies such as NIH in USA and EMBL in Europe, Bioinformatics became more and more prominent and diverse ... Therefore, reducing the costs and shortening the new drug development time would be a stimulator for the pharmaceutical industry CHAPTER Figure 1.2 The process of new drug development for African...

Ngày tải lên: 17/09/2015, 17:19

... opportunity to be a part of their labs, for their intellectual and moral support, for their inspiration in doing good science, and for teaching me how to present myself and my work I would like to show ... committee members Professor Fred Wong and Professor Marie Clement for their invaluable comments and suggestions for my thesis, and to my collaborators, Dr Damien Thévenin and Professor Donald Engelman ... mates and colleagues in Singapore – Dr Yamada Yoshiyuki, Prashant, Tze Khee, Kai Sen, Anandi and Dr Orsolya Kiraly- for lending me support in my projects, for giving me constructive suggestions, and...

Ngày tải lên: 30/09/2015, 06:20

... control of APIs and drug products and also develop and validate appropriate methods These methods are routinely used by manufacturing companies in process testing and by authorities for the quality ... generally available and applicable for most users The development of sample preparation from complex drug products is the most challenging area of assay method development for HPLC To demonstrate ... solution was performed until the colour of the solution changed from orange to green and remained green for at least 3.3 Instruments and other equipment For mobile phase degassing and sample sonication...

Ngày tải lên: 10/12/2016, 13:40

... the case when only a one-side aligner is available and the thermal SiO2 is used as the mask material The point will be especially useful for MEMS devices fabricated by etching through whole silicon ... etch rate and etched surface roughness were carried out Based on the obtained results, the optimal conditions for etching silicon in TMAH were suggested Finally, a useful procedure for fabricating ... orientation, n-type, and polished double side, phosphorous doped with sensitivities of - 20 Ω.cm, 260 ± 25 µm thickness were used for experiments The protection material was only SiO2 layer formed on two...

Ngày tải lên: 13/02/2014, 04:20

... George Vadas for always being available for advice and, even more so, assistance I am forever grateful for the many times that George went above and beyond to provide me help in acquiring and interpreting ... grateful for having, not one, but two great and equally dedicated advisors, Dr Stephen Kaattari and Dr Michael Unger Their mentorship, patience, and enthusiasm for science are values I will forever ... molecules for antibody development Characterization of a monoclonal antibody (mAb) to dibenzothiophene revealed specificity for to 5-ring PAHs and heterocycles Therefore, the goals of antibody development...

Ngày tải lên: 13/05/2014, 14:01

... N + Furthermore, for each m ≥ N + the right-hand side of Eq (21) leads to the three-term recursion relation (8a) and (8c) for the coefficients cn and sn Therefore the right-hand side of Eq (21) ... enough material for him or her to appreciate the advantages of the J -matrix method, and to encourage its use and development into a viable method for theoretical calculation of nuclear and atomic ... perform a calculation, we need merely augment this N × N matrix with the extra row and column shown and with the right-hand side driving term Equation (22) can be immediately solved for t by standard...

Ngày tải lên: 27/06/2014, 05:20

... HETEROCYCLES Part 2A: DEVELOPMENT AND APPLICATION OF HANTZSCH ESTER Part 2B: DEVELOPMENT AND APPLICATION OF A HYPERVALENT IODINE REAGENT CHE JUN (B.Sc., Soochow University) A THESIS SUBMITTED FOR THE DEGREE ... ketones and alkylidene malonic diesters is a rapid and experimentally simple procedure for the preparation of saturated ketones and alkyl malonic diesters vii Part 2b is Development and Applications ... Woen Susanto, and Wong Lingkai for their help and encouragement during my research I would also like to thank staff from the NMR, MS, Chromatography laboratories and chemical supplies for their patience...

Ngày tải lên: 11/09/2015, 10:15

... (LPME) features the use of microlitres of organic solvent for the extraction and enrichment of analytes This work focuses on the development and application of two kinds of liquidphase microextraction ... Different modes of CE 102 4.1.4 Application of CE to the analysis of drugs and pollutants 103 4.1.5 Off-line and on-line concentration techniques for capillary electrophoresis ... amount of toxic solvents and potential for error during the multi-transfer and operational procedures Therefore, new sample preparation methods which are less labor-intensive, afford less exposure...

Ngày tải lên: 17/09/2015, 17:20

... 4-methylacetophenone SDME was further studied and developed by Jeannot and Cantwell [48,49], He and Lee [50], and Jager and Andrews [51] In SDME, based on passive diffusion [52] and a great reduction of the extractant ... while operating under higher pressures and temperatures PLE is fast and efficient, and requires only a small amount of solvent, but purchase and maintance costs for equipment is very high [12] The ... organic solvent and analyzed by GC–MS Good linearity and limits of detection were obtained They [34] reported that no analyte carryover was observed, and the µ-SPE device could be used for up to 30...

Ngày tải lên: 30/09/2015, 06:20