drug and gene therapy mediated by physical methods
Intravesical tumor necrosis factor alpha gene therapy mediated by a novel liposome system in an orthotopic murine bladder cancer model
... 1.3.3 Gene therapy strategies for bladder cancer 12 1.3.3.1 Immune inductive gene therapy 12 1.3.3.2 Corrective gene therapy 13 1.3.3.3 Cytotoxic gene therapy 14 1.3.3.4 Anti-sense oncogene therapy ... cellular oncogenes and tumor suppressor genes in cancer cell Gene therapy is defined as the treatment of an acquired or inherited disease by direct transfer of genetic material and genetic modification ... common strategies in gene therapy of bladder cancer are immune inductive, corrective, cytotoxic gene therapy and anti-sense oncogene therapy 1.3.3.1 Immune inductive gene therapy Immune inductive...
Ngày tải lên: 08/11/2015, 16:45
ethical issues in molecular medicine and gene therapy
... in the case of gene therapy as well Appropriate Candidates for Gene Therapy We now turn to another dimension of ethics issues in gene therapy, namely, who the candidates are for therapy We distinguish ... true in the case of Donald and the gene therapy clinical trial we are considering for his CF? 326 ETHICAL ISSUES IN MOLECULAR MEDICINE AND GENE THERAPY In the case of gene therapy there appear to ... The Lives to Come: The Genetic Revolution and Human Possibilities Simon and Schuster, New York, 1996 346 ETHICAL ISSUES IN MOLECULAR MEDICINE AND GENE THERAPY Kolata G Genetic defects detected...
Ngày tải lên: 10/04/2014, 22:10
molecular medicine and gene therapy. an introduction
... of some genes is up-regulated and of other genes down-regulated (Adapted from Anderson and Anderson, Electrophoresis, 1996.) MOLECULAR MEDICINE AND GENE THERAPY: AN INTRODUCTION gene and the ... between the expanding universe of therapeutic options and the genetic constitution of an individual patient GENE THERAPY AND MOLECULAR MEDICINE A simple and concise definition of gene therapy (there ... goal of somatic (nongermline) gene therapy is to achieve a healthy phenotype by manipulating gene expression Gene therapy, thereby, corrects or compensates for genetic lesions or deficiencies...
Ngày tải lên: 10/04/2014, 22:10
components of cell and gene therapy
... neurodegeneration and provide a way to test gene therapy strategies for CNS disorders, injury, or aging 210 COMPONENTS OF CELL AND GENE THERAPY FOR NEUROLOGICAL DISORDERS NEUROTROPHIC FACTORS AND GENE ... Neurological Disorders and Brain Tumors Humana, Totowa, NJ, 1998 Doering LC Gene therapy and neurodegeneration Clin Neurosci 3:259–321, 1996 Kaplitt MG, Loewy AD Viral Vectors, Gene Therapy and Neuroscience ... OF CELL AND GENE THERAPY FOR NEUROLOGICAL DISORDERS CLINICAL TRIALS TESTING GENETICALLY MODIFIED CELLS AND NEUROTROPHIC FACTORS FOR NEURODEGENERATION Therapeutic options for human neurodegeneration...
Ngày tải lên: 10/04/2014, 22:11
An Introduction to Molecular Medicine and Gene Therapy - part 3 ppt
... AAV vectors can be generated by removing viral genes and replacing them with a promoter and therapeutic gene They can be produced in cells expressing the AAV rep and cap genes and that have been ... strand hybridizes to the first DNA strand in the PBS region (i) Completion of the first and second strands RT copies the remainder of the first and the second DNA strands, to generate a double-stranded ... (insulitis) by T and B lymphocytes, 74 BUILDING A BETTER MOUSE: GENETICALLY ALTERED MICE AS MODELS FOR GENE THERAPY • dendritic cells, and macrophages precedes autoimmune destruction of b cells and diabetes...
Ngày tải lên: 10/08/2014, 14:22
An Introduction to Molecular Medicine and Gene Therapy - part 4 ppsx
... proteins or genes, are additive, that is, the defective gene (or gene product) is supplemented by the therapeutic drug while the defective gene and its products are ignored The gene addition” ... events can cause genetic dysfunction by disrupting active genes, and in rare instances random integration may lead to the activation of qui- 116 GENE TARGETING escent genes by positioning a strong ... directly by a cause -and- effect correlation Such relationships are invaluable for gene therapy strategies as well as defining the function of new genes GENE TARGETING: THE FUTURE Gene therapy is...
Ngày tải lên: 10/08/2014, 14:22
An Introduction to Molecular Medicine and Gene Therapy - part 6 ppsx
... neurodegeneration and provide a way to test gene therapy strategies for CNS disorders, injury, or aging 210 COMPONENTS OF CELL AND GENE THERAPY FOR NEUROLOGICAL DISORDERS NEUROTROPHIC FACTORS AND GENE ... Medicine and Gene Therapy Edited by Thomas F Kresina, PhD Copyright © 2001 by Wiley-Liss, Inc ISBNs: 0-471-39188-3 (Hardback); 0-471-22387-5 (Electronic) CHAPTER Components of Cell and Gene Therapy ... gene therapy Ann Thorac Surg 68:1924–1928, 1999 Amant C, Berthou L, Walsh K Angiogenesis and gene therapy in man: Dream or reality Drugs 59(Spec No 33–36), 1999 Ponder KP Systemic gene therapy...
Ngày tải lên: 10/08/2014, 14:22
An Introduction to Molecular Medicine and Gene Therapy - part 7 pdf
... potential candidates for gene therapy DNA Repair Genes Genetic defects in double-stranded DNA can be repaired by the products of DNA repair genes These gene products act to proofread and correct ... both viral and nonviral vectors Tumor targeting and specificity need to be advanced and a further understanding of gene regulation, apoptosis, and the synergy between gene therapy and chemotherapy ... Ras and leukemia: From basic mechanisms to gene- directed therapy J Clin Oncol 17:1071–1079, 1999 Prodrug Cancer Therapy Connors TA The choice of prodrugs for gene directed enzyme prodrug therapy...
Ngày tải lên: 10/08/2014, 14:22
An Introduction to Molecular Medicine and Gene Therapy - part 10 pot
... study, 309 Gene therapy vectors, gene transfer, 16 Genetic counseling, 348 Genetic diseases, 2–4 Genetic disorders: monogenetic, 4–5 multifactorial, 5–7 379 Genetic maps, personalized, 348 Genetic ... tumor suppressor gene augmentation, 245 use for gene therapy, 101–102 use of sequences for gene transfer, 100–101 Aging, 19–20 gene therapy, 14 Alcoholic liver disease, hepatic gene therapy, 177–178 ... healthy and diseased tissue On the one hand, these gene profiling techniques will detect gene therapy targets—genes whose products contribute to disease On the other hand, they will identify genes...
Ngày tải lên: 10/08/2014, 14:22
Báo cáo y học: "Anti-inflammatory activity and neutrophil reductions mediated by the JAK1/JAK3 inhibitor, CP-690,550, in rat adjuvant-induced arthritis" ppsx
... between inhibition of JAK1 and JAK3, efficacy, and the inhibition of inflammatory cytokines and neutrophilia in the rat AIA model with CP-690,550 (Tables and 2, Figures and 2) Increases in arthritis, ... pharmacokinetic and pharmacodynamic modeling was used to determine the drug concentration-effect relationship between JAK kinase inhibition and neutrophil reductions in the non-clinical and clinical ... CP-690,550 mediated reduction in PBNCs in the rat AIA model, and likely in human RA patients, is due to the antiinflammatory action of the compound by the Page of 12 suppression of cytokines and chemotactic...
Ngày tải lên: 11/08/2014, 03:20
COAXIAL DOUBLE WALLED MICROSPHERES FOR DRUG AND GENE DELIVERY APPLICATIONS
... blank and free drug groups as well as blank and drug- loaded microsphere groups The cell nuclei were stained by Hoechst dye and indicated in blue The p53 was stained by DyLightTM 549 dye and indicated ... nanoparticles (chi-p53, gene encoding p53 tumor suppressor protein) and/ or doxorubicin in the shell and core phases, respectively, for combined gene therapy and chemotherapy The microspheres ... three and five days after commencement of treatment The groups include blank and free drug (FD) groups (blank, chi-p53 FD, Dox FD, and combined Dox and chi-p53 FD) as well as blank and drug- loaded...
Ngày tải lên: 10/09/2015, 09:05
Gene Therapy - Tools and Potential Applications by Francisco Martin Molina Contributors ppt
... Yahan Fan and Jian Wu Chapter DNA Electrotransfer: An Effective Tool for Gene Therapy 109 Aurore Burgain-Chain and Daniel Scherman Chapter siRNA and Gene Formulation for Efficient Gene Therapy ... genes in the desired cell population In this chapter we explain the concept and aim of gene therapy, the different gene delivery systems and therapeutic strategies, how genes are deliv ered and ... important, and most difficult, challenge in gene therapy is the issue of delivery The tools used to achieve gene modification are called gene therapy vectors and they are the key for an efficient and...
Ngày tải lên: 17/03/2014, 21:20
Novel Gene Therapy Approaches Edited by Ming Wei and David Good pdf
... Introduction Gene therapy is a promising approach to treat intractable and refractory diseases at the genetic level Basically, in gene therapy, target gene expression is induced by delivering foreign genes ... depletion on the pharma‐ cokinetics and gene delivery mediated by cationic lipid-DNA complexes Human Gene Therapy 1998;9(3) 315-323 [56] Tandia BM, Vandenbranden M, Wattiez R, Lakhdar Z, Ruysschaert ... transfer mediated by lipo- and polyplexes Molecular Therapy 2005;12(3) 468-474 [112] Rejman J, Conese M, Hoekstra D Gene transfer by means of lipo- and polyplexes: role of clathrin and caveolae-mediated...
Ngày tải lên: 23/03/2014, 14:20
gene therapy of cancer, methods and protocols
... Molecular Biology and Gene Therapy Protocols, edited by Andrew H Baker, 1999 29 DNA Vaccines: Methods and Protocols, edited by Douglas B Lowrie and Robert Whalen, 1999 28 Cytotoxic Drug Resistance ... Cells, edited by Mark J Jaroszeski, Richard Heller, and Richard Gilbert, 2000 36 Septic Shock Methods and Protocols, edited by Thomas J Evans, 2000 35 Gene Therapy of Cancer: Methods and Protocols, ... Protocols, edited by Wieslaw M Kazmierski, 1999 22 Neurodegeneration Methods and Protocols, edited by Jean Harry and Hugh A Tilson, 1999 21 Adenovirus Methods and Protocols, edited by William S M...
Ngày tải lên: 11/04/2014, 09:42
nonviral vectors for gene therapy, methods and protocols
... J L., and Velander, W H (1995) Transgenesis in mice by cytoplasmic injection of polylysine/DNA mixtures Transgenic Res 4, 353–360 Wu, G Y and Wu, C H (1987) Receptor -mediated in vitro gene transformation ... recognize and internalize HA (16,17) More than 90% of HAs in the blood stream are known to be taken up and metaboFrom: Methods in Molecular Medicine, vol 65: Nonviral Vectors for Gene Therapy Edited by: ... Nazarova, O., and Seymour, L W (1996) Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov,...
Ngày tải lên: 11/04/2014, 09:59
suicide gene therapy, methods and reviews
... induced by the Varicella zoster and Herpes simplex viral thymidine kinases in suicide gene therapy Cancer Gene Ther 7, 1456–1468 83 Kaneko, Y and Tsukamoto, A (1995) Gene therapy of hepatoma: bystander ... Vol 90, Suicide Gene Therapy: Methods and Reviews Edited by: C J Springer © Humana Press Inc., Totowa, NJ Niculescu-Duvaz and Springer (VDEPT) (3), or gene prodrug activation therapy (GPAT) (4) ... L., and Springer, C J (1999) Prodrugs for antibody- and gene- directed enzyme prodrug therapies (ADEPT and GDEPT) Anticancer Drug Des 14, 517–538 23 Springer, C J and Niculescu-Duvaz, I (2002) Gene- directed...
Ngày tải lên: 11/04/2014, 10:33
Báo cáo hóa học: " Development of targeted therapy for bladder cancer mediated by a double promoter plasmid expressing diphtheria toxin under the control of H19 and IGF2-P4 regulatory sequences" potx
... group and others, the transcriptional regulatory sequences of the H19 and IGF2 genes emerged as candidates for cancer targeted therapy H19 and IGF2 (the human P3 and P4 promoters) are onco-fetal genes ... by ISH and by qRT-PCR qRT-PCR and ISH techniques were applied to determine and quantity the level of H19 and IGF2-P4 in human TCC samples Human TCC samples (n = 29) were examined by qRT-PCR and ... the patient Cancer Therapy 2005, 3:249-266 Cooper MJ: Noninfectious gene transfer and expression systems for cancer gene therapy Semin Oncol 1996, 23:172-187 Kouraklis G: Gene therapy for cancer:...
Ngày tải lên: 18/06/2014, 16:20
Báo cáo sinh học: "Gene therapy with tumor-specific promoter mediated suicide gene plus IL-12 gene enhanced tumor inhibition and prolonged host survival in a murine model of Lewis lung carcinoma" pptx
... of a therapeutic gene to cancer cells Gene directed enzyme/prodrug therapy (GDEPT) or suicide gene therapy using viral vectors is an attractive alternative approach to cancer therapy, with the ... of T cells and NK cells and initiating Th1-type immune responses by activation of CD4+ and CD8+ cells [16] In the present study, we investigated a combined target suicide gene therapy and immunomodulating ... therapeutic efficiency of target suicide gene therapy mediated by hTERT promoter in combination with immuno -gene therapy in a murine model of LLC both in vitro and in vivo Xu et al Journal of Translational...
Ngày tải lên: 18/06/2014, 19:20