dqasomes as mitochondria targeted nanocarriers for anti cancer drugs

... Nanotechnology for Cancer Therapy is illustrated by such novel classes of nanocarriers as DQAsomes, which are mitochondrial -targeted nanovectors capable of delivery of anti- cancer drugs as well as carriers ... metastasis This realization and the accompanied potential for development of successful cancer therapeutics, as well as for tumor imaging strategies based on tumor-associated vasculature, has ... Nanoparticles as Delivery Vehicles for Anti- Cancer Agents 777 Andras G Lacko, Maya Nair, and Walter J McConathy Chapter 38 DQAsomes as Mitochondria- Targeted Nanocarriers...

Ngày tải lên: 04/06/2014, 14:30

... and reporter gene activity was assessed Luciferase activity was measured using the Promega kit 'Luciferase Assay System' (E-1500; Promega, Madison, USA) Light output was detected using a Lumac ... content was measured by the Bio-Rad (Hercules, CA, USA) protein assay reagent, and the results were expressed as light units/μg protein LucSV40 (Luc-4) was used as a reference for maximal luciferase ... ml flask Immunohistochemistry (IHC) Following fixation IHC was performed on the isolated ascites cells The LEVEL1 PEROXIDASE ANTI- PEROXIDASE (PAP) DETECTION SYSTEM and the CA-125 monoclonal antibody...

Ngày tải lên: 18/06/2014, 15:20

... The ISH was performed on successive slides of TCC tissue for H19 and IGF2-P4 transcripts The intensity of hybridization signal was indicated as (0) for no staining, (+1) for weak, (+2) for moderate ... experiment was stopped after 48 hours Luciferase activity The cells were harvested and the luciferase activity was determined using the luciferase Assay System kit (Promega) The light output was measured ... 48 hours and luciferase activity was assessed The decrease in LucSV40 activity was determined by comparison to the same cell type transfected with LucSV40 alone as a measure for cytotoxicity The...

Ngày tải lên: 18/06/2014, 16:20

... Activation of NF-jB has been implicated in cellular transformation, tumor promotion, angiogenesis, and tumor invasion and metastasis.17 One mechanism that may play a role in the anticancer properties ... curcumin as high as 15 lM together with low-frequency ultrasound on two OSCC cell lines (OSCC-4 and OSCC-25) The ultrasound-enhanced delivery of curcumin as a cytotoxic agent for OSCC was found ... screening for oral squamous cell carcinomas Evidence-based clinical recommendations regarding screening for oral squamous cell carcinomas J Am Dent Assoc 2010;141(5):509–20 Warnakulasuriya S...

Ngày tải lên: 01/07/2014, 13:23

... Aldehyde Dehydrogenase ALDH1A1 Aldehyde Dehydrogenase class A1 (cytosol) ALDH2 Aldehyde Dehydrogenase class (mitochondria) ALDH3A1 Aldehyde Dehydrogenase class A1 (cytosol) ... in cancer chemoresistance ALDH3A1 was originally designated as the tumor ALDH, as it was found highly expressed in some human tumors such as hepatoma, lung adenocarcinoma, myeloma, breast cancer ... This is one of the reasons for lower alcoholism rate in Asian populations (Luczak et al., 2002) Studies have shown the association of ALDH2*2 with an increased risk for various cancers, including...

Ngày tải lên: 24/08/2014, 09:51

... varieties of these biopharmaceuticals as a cure for cancer By far, protein therapeutics has been the most successful class of biopharmaceutical drugs for cancer therapy with approximately one ... doxorubicin (Myocet) which has shown less cardiotoxicity than doxorubicin for the treatment of metastatic breast cancer [8], and PEG-L-asparaginase [9, 10] with significantly longer plasma half life than ... used for the codelivery of various anti- cancer drugs and therapeutic proteins for improved cancer therapy The first part of this thesis focuses on the evaluation of these cationic nanoparticles as...

Ngày tải lên: 30/09/2015, 05:59

... caspases The initiator caspases include caspase 8, 9, 10 and the effector caspases include caspase 3, 6, The initiator caspases function to cleave and activate effector caspases Effector caspases ... such as Fas-associated death domain (FADD), which interacts with pro-caspase and 10 to form the death-inducing signaling complex (DISC) Pro-caspase and 10 are self-cleaved Activated caspase and ... known as death receptor (DR)) on plasma membrane Both intrinsic and extrinsic pathways are mediated by caspases Caspases are intracellular cysteinyl-aspartate-specific proteases which exist as pro-enzymes...

Ngày tải lên: 11/09/2015, 10:02

... Albanell, J & Baselga, J Trastuzumab, a humanized anti- HER2 monoclonal antibody, for the treatment of breast cancer Drugs Today 35, 931–946 (1999) 29 Ferrara, N VEGF as a therapeutic target in cancer ... screening for conformational-sensitive targeting agents such as affibodies, avimers and nanobodies, as well as intact antibodies and their fragments process, scFv antibodies have been identified for ... blocks such as proteins Abraxane is an albumin-bound paclitaxel nanoparticle formulation approved by the FDA in 2005 as a second-line treatment for metastatic breast cancer Abraxane was designed...

Ngày tải lên: 28/05/2014, 15:07

... important for rapidly dividing tissue, like the colonic mucosa Therefore, the cancer risk associated with low folate intake is probably higher for colon cancer than for breast cancer Most of the breast ... ↓Breast cancer N-6 fat content signif higher in cases For given level of N-6 fat, EPA and DHA had a protective effect ↑DHA = ↓Breast cancer No associations between N3:N-6 ratio and breast cancer ... ↑risk of breast cancer (OR = 0.55, P-trend = 0.001) Folate intake not associated with overall risk of breast cancer Folate intake not associated with overall risk of breast cancer No association...

Ngày tải lên: 06/03/2014, 02:21

... prostate-specific antigen for prostate cancer, cancer antigen CA19-9 for gastrointestinal cancer and CA-125 for ovarian cancer, lack sufficient specificity and sensitivity for use in early cancer diagnosis ... hunt for relevant autoantibodies has intensified in recent years, as evidenced by a search for ‘autoantibodies and cancer on PubMed Autoantibodies and TAAs have been found many cancers such as ... autoantigen assays, the sensitivity was between 8% and 34% for primary breast cancer and between 3% and 23% for DCIS The specificity was between 91% and 98% for both types of breast cancer However, as...

Ngày tải lên: 23/03/2014, 04:20

... trial phase Type of cancer Combined with Phase Phase Phase Phase Phase Metastatic colon cancer Pancreatic cancer Osteosarcoma Colorectal cancer Stage IV breast cancer Gemcitabine Gemcitabine Phase ... intraepithelial neoplasia Lung cancer Bronchial dysplasia Prostate cancer High-grade prostatic intraepithelial neoplasia Breast cancer Nonmetastatic bladder cancer Prostate cancer Green tea, decaffeinated ... decaffeinated black tea II I–II I I Phase I–II Phase I–II Phase II Phase Phase Phase Phase Tea polyphenols and theaflavins III III I–II II II II II II II Phase II Phase II Phase II FEBS Journal 275 (2008)...

Ngày tải lên: 30/03/2014, 02:20

... maintained for at least weeks with no appearance of new disease CR + PR was rated as the overall response rate Stable disease (SD) was defined as a less than 50% reduction or less than 25% increase ... complete response (CR) was defined as the disappearance of all lesions and no appearance of new disease for at least weeks Partial response (PR) was defined as a reduction by at least 50% in the sum ... dexamethasone mg bid 24 hours before each infusion of docetaxel up to a total of six doses Treatment was given for a maximum of cycles and was discontinued in cases of unacceptable toxicity, disease...

Ngày tải lên: 18/06/2014, 15:20

... model of human colon cancer Thus, this vector has potential for redirection of human PBLs to chimeric anti- tumor T cells, forming basis for immunotherapy of many different human cancers Mouse cells ... thrust of the current work was to show that high efficiency stable transduction of human PBLs is a feasible prospect for generating anti- cancer chimeric T cells for use in cancer immunotherapy The ... types and this approach to cancer therapy has been further restricted by toxicity associated with the need for exogenous administration of interleukin-2 A prominent reason for failure of both vaccination...

Ngày tải lên: 19/06/2014, 08:20

... model of human colon cancer Thus, this vector has potential for redirection of human PBLs to chimeric anti- tumor T cells, forming basis for immunotherapy of many different human cancers Mouse cells ... thrust of the current work was to show that high efficiency stable transduction of human PBLs is a feasible prospect for generating anti- cancer chimeric T cells for use in cancer immunotherapy The ... types and this approach to cancer therapy has been further restricted by toxicity associated with the need for exogenous administration of interleukin-2 A prominent reason for failure of both vaccination...

Ngày tải lên: 20/06/2014, 04:20

... from inner phase and then slower release Nevertheless, the release rate of mixed micelles was faster than that of CLE before the first 12 h, the release rate of the two formulations was comparable ... release medium was taken and replaced by fresh medium The content of propofol in the medium was measured by HPLC method as described above The cumulative release percentage of propofol was calculated ... in the aqueous phase of the micelles and CLE, separation of the two phases was performed using ultracentrifugation Ultracentrifugation was performed at 10,000 rpm (14000 g) for 20 Amicon ultra...

Ngày tải lên: 21/06/2014, 04:20

... micelles, the ethaselen-loaded micelle solution was lyophilized using PEG6000 as a lyoprotectant, and then dissolved in DMSO by ultrasonication for 15 min, and ethaselen content was measured with ... were then calculated based on the following formula: LCð%Þ mass of ethaselen extracted from freezeÀdried micelles ¼ total mass of freezeÀdried micelle Â100% EE ð%Þ mass of ethaselen extracted from ... this micelle-based drug delivery system could reduce unwanted side effects of anticancer drugs during cancer therapy Overall, ethaselen-loaded polymeric micelle possessed improved antitumor activity...

Ngày tải lên: 22/06/2014, 00:20

... Fulvestrant; E = Exemestane; L = Letrozole; MBC = Metastatic Breast Cancer; LAPC = Locally Advanced Primary Breast Cancer; PR = Partial Response; SD = Stable Disease; DoCB = Duration of Treatment in patients ... CK, Shou J, Massarweh S, Schiff R: Crosstalk between Estrogen Receptor and Growth Factor Receptor Pathways as a Cause for Endocrine Therapy Resistance in Breast Cancer Clinical Cancer Research ... Nicholson RI: Automated quantitation of immunocytochemically localized estrogen receptors in human breast cancer Cancer Res 1990, 5012:3545-3550 British Breast Group: Assessment of response to...

Ngày tải lên: 09/08/2014, 02:21

... end-diastolic dimension (EDD) and end-systolic dimension (ESD) were measured In addition, the percentage of fractional shortening (FS) was calculated All measurements were performed in a blind fashion ... transcriptase reaction using TaqMan High capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA) was performed for cDNA synthesis The cycling parameters were 25°C for 10 minutes and 37°C for ... concentration was calculated from standard curves The expression was determined as the ratio of the RNAtarget/ RNAGusB Positive cells for CD3, CD8 and CD 20 Immunohistochemical staining was performed...

Ngày tải lên: 10/08/2014, 09:21

... Histone deacetylase inhibitors in cancer treatment Anticancer Drugs 2002, 13:1-13 53 Mottet D, Castronovo V: Histone deacetylases: target enzymes for cancer therapy Clin Exp Metastasis 2008, 25:183-189 ... monitoring for QTc prolongation was performed Among 1578 ECGs analyzed, there has been no QTc >500 ms, one QTc >480 ms, and one QTc >60 ms increased from baseline Best overall response is PR for patients, ... cell-cycle G1 phase accumulation and apoptosis of K562 cells and acute leukemia MV4-11 cells [102] The anti- tumor effect by LBH589 was also demonstrated in multiple myeloma, NSCLC as well as castrate-resistant...

Ngày tải lên: 10/08/2014, 22:20