... and police for everything from underage drinking to hit and runs. Be careful what you post in Facebook just like everywhere else online. Effective Internet Presence: Now required for success in business ... developing an Effective Internet Presence, including upcoming teleseminars and hopefully another ebook or two on the subject. More information about Ted is available via Google, or: Internet Business ... you think you deleted it. For example, the “Internet Wayback Machine” and Google cache contain copies of “deleted” information. “Unlike footprints left in the sand at the beach, our online data...
Ngày tải lên: 14/02/2014, 04:20
... obtain sufficient funding from other sources. Before applying to the Postgraduate Funding Office for funding, you must submit your UCT application (Form 1PG). Information and Application form ... procedures 3 PART B: ã Applying online for admission in 2013 8 PART C: ã Completing a paper application for admission in 2013 9 ã Codes you will use when completing a paper application form 11 Contact ... these documents online, that each document is scanned and uploaded as a single document. The additional information/documets required for each qualifiaction is indicated in the following pages in...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf
... media containing Geneticin to select for cell containing vector DNA (as in Fig. 5A,G). After three weeks, the number of cells was determined by fixing cells and staining with dye. Individual colonies ... plated in media containing Geneticin to select for cell containing vector DNA. After three weeks, the number of cells were determined by fixing cells and staining with dye and quantified in (H). ... cell lines to determine whe- ther the gene product had protective effects from DCA damage in vitro. Cellular stress protein responses play a key role in minimizing cell injury and maintaining tissue...
Ngày tải lên: 23/03/2014, 10:21
the identification and characterization of proteins required for endocytosis in the budding yeast saccharomyces cerevisiae
Ngày tải lên: 14/11/2014, 06:20
How to pass the GMAT unbeatable preparation for success in the graduate management admission test
... non-scoring, as they are being trialed for inclusion as scoring items in future tests. You are not given any indication as to which are scoring, so treat them all with the same determination. Things ... should find to spend preparing for the challenge. The GMAT đ is very much a US test and one that examines key features covered in a good US formal education. If it is some years since you left formal education, ... guessing right. Guessing plays an important part in many GMAT đ candidates test-taking strategies, especially in the last part of each sub-test when time may be running out. Key stages in preparing...
Ngày tải lên: 13/02/2014, 23:28
Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
... Nop53p binds 5.8S rRNA. UV cross-linking was performed after incubation of 1 pmol of in vitro transcribed, uniformly labeled 5.8S rRNA with increasing amounts of His–Nop53p or bovine serum albumin ... (generating 5.8S rRNA) or pBluescript (nonspecific RNA) in the presence of 10 mm NTPs. UV cross-linking was performed by placing RNA–protein complexes on ice and irradiation for 15 min at 260 nm using ... that include increased primer extension products at certain box C ⁄ D methylation sites, indicating that Nop17p is required for proper pre-rRNA methylation [28]. A third Nop17p-interacting partner...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... regions in strains lacking either or both the Swi1 (residues 329– 655) and Snf5 (residues 2–327) activator-binding domains, 30 min after galactose induction. Quantifying recruitment in relation ... Protein A Agarose ⁄ Salmon Sperm DNA incubation for no more than 1 h. Beads were washed twice for 15 min in lysis buffer containing 150 m m NaCl, followed by a 15-min wash in lysis buffer containing 500 ... critical for normal galactose induction of the tested genes. Interestingly, the strains lacking either the Fig. 3. The activator-binding domains of the SWI ⁄ SNF complex are required for its recruitment...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
... of the gastrin–transferrin inter- action is still unknown. Knowledge of the regions of transferrin required for the binding of gastrin, and of the regions in gastrin required for the interaction ... is required for the Ggly–trans- ferrin interaction, to define the domains ⁄ residues of Ggly involved in the interaction (using Ggly mutants), and, finally, to determine the regions of transferrin required ... connection between gastrins and iron homeostasis was first provided in a search for gastrin- binding proteins in porcine gastric mucosa [14]. An interaction between Gamide and transferrin was identi- fied...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
... obtained, as discussed in detail below, indicate a crucial role for sulfur–sulfur interactions in defining the catalytically competent binding mode of CoA in the active site. The pantetheine binding ... for increasing the affinity of binding, as well as the solub- ility of the substrate. Interestingly, the side chain of Ser247 points away from SPP, as previously seen in unliganded thiolase; in the ... used, being identical to SPP except for the replacement of the terminal sulfur atom by oxygen, the binding mode of the ligand also changes, resulting in a nonproductive binding mode. Our data indicate...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
... lgặmL )1 spectinomycin. For thylakoid isolation and physiological measurements, wild-type and transformed plants (originating from two independent transplastomic lines) were planted in compost and kept in ... was explained by a displacement of this domain. Thus, PSI-J does not appear to participate directly in binding of Pc or Cyt c 6 , but plays a role in maintaining a precise recognition site for the ... the 20% increase in the pool of inactive PSI complexes in the absence of PSI-J does not explain the dramatic reduction in PSI activity measured by NADP + photoreduction activity. The kinetic analysis clearly...
Ngày tải lên: 16/03/2014, 11:20
A Model Course for Public Health Education in Chiropractic Colleges: A Users Guide doc
... spine surgeons (38)! Another study found that chiropractors had sought out additional training in preventive health services, suggesting a need for increases in such training on the undergraduate ... levels of provider training (40,41). This information gives an indication that some Chiropractors already do perform many of the recommended screening and counseling clinical preventive services ... paradigm” thus reducing this type of problem. Numerous authors and educators have called for public health and clinical preventive services education to be incorporated into already existing class work,...
Ngày tải lên: 22/03/2014, 15:20
Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf
... role of Gly residues in the 599–611 loop in positioning the Tyr605 and Tyr612 residues needed for substrate hydrolysis. In addition, our data indicate that Tyr605 is respon- sible for transition ... were incubated for 30 min and the products were evaluated by HPLC. Two products with an absorbance at 330 nm were detected, suggesting a single cleavage site in the MCA substrate. Extended incubation ... have produced a model for the closed form of TOP with bound substrate. The model allows for a more careful analysis of the resi- dues in close proximity to the bound substrate in TOP, including Tyr612...
Ngày tải lên: 23/03/2014, 06:20
Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
... either tyrosine kinase inhibitor, genistein, or MAP kinase inhibitor, PD98059. Inhibition of tyrosine kinase by genistein greatly inhibited LPA-induced PAK activation, whereas MAP kinase activity ... actin stress fibers and to the assembly of focal adhesions [15]. Activated FAK in turn recruits other proteins, including paxillin, p130 Cas , vinculin and talin in focal adhesions. These protein ... factors and during formation of focal adhesions and spreading [14]. GPCR agonists including LPA appear to induce tyrosine phos- phorylation of FAK via a Rho-dependent pathway that leads to the formation...
Ngày tải lên: 30/03/2014, 13:20
Báo cáo khoa học: Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system potx
... activator protein (lane 11), indicating that the Mediator is involved in activated transcription. Neither the inclusion of human recombinant TFIIA nor the Gal4 DNA binding domain had an effect ... by centrifugation, and the insoluble pellet containing the recombinant protein was washed twice with buffer A containing 0.05% (v/v) sodium deoxycholate and 0.1% (v/v) Triton X-100. Recombinant proteins were extracted from ... of transcription were obtained in the whole-cell extract without (lane 1) or with (lane 2) Gal4 DNA binding domain and in the reconstituted assay with Gal4 binding domain (lane 3). We also tested...
Ngày tải lên: 30/03/2014, 14:20
SUPERVISORY, INSTRUCTIONAL SKILLS AND PROFESSIONAL PERFORMANCE OF TEACHERS IN VOCATIONAL COLLEGES IN NGHE AN PROVINCE: BASIS FOR DEVELOPING AN ENHANCEMENT PROGRAM
... teachers in supevision of the classroom instruction. technical skills refer to skills that include Speaking, Writing, Demonstrating Outlining/Planning, Computing, Listening, Chairing a Meeting skills. ... superiors, initiating seminars/trainings, rewards and recognition, conducting periodic performance evaluation among teachers, participation of teachers in formulating school policies, organizing programs ... networks have been innovated and developed, moving from low-level vocational training system with two levels of training into the vocational training system with three levels of training: primary,...
Ngày tải lên: 13/05/2014, 14:46
Báo cáo hóa học: " Human cytomegalovirus UL27 is not required for viral replication in human tissue implanted in SCID mice" ppt
... and this recombinant virus contains a deletion that eliminates the amino terminal 80% of the open reading frame starting just upstream of the start codon (coordinates 33021–34565 in X17403). Two ... mg/kg), xylazine (15 mg/kg) and the topical anesthetic propa- racaine-HCL (0.5%) was instilled in the eyes. A winged infusion needle containing mechanically dissociated human fetal retinal tissue ... provided recom- binant viruses and provided critical intellectual input. ERK provided critical intellectual input. Acknowledgements The authors thank Caroll Hartline and for titering virus samples,...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo sinh học: "Nuclear localization is required for Dishevelled function in Wnt/ -catenin signaling" pps
Ngày tải lên: 06/08/2014, 18:21