dna vaccines for infectious diesase
Development of DNA vaccines for allergic asthma
Ngày tải lên :
15/09/2015, 17:09
... treatments under development 19 1.3 DNA vaccines for prevention and treatment of allergic asthma 26 1.3.1 History of vaccine and DNA vaccine 26 1.3.2 DNA vaccines for allergic asthma and other allergic ... evaluation of the Der p DNA immunization for potential prophylactic and therapeutic applications for allergic asthma were performed using an experimental mouse model for allergic asthma Results ... of DNA immunization 216 6.3.2.1 Therapeutic effect of DNA vaccine on Der p specific IgE 217 6.3.2.2 Therapeutic effect of DNA vaccine on T cell polarization 217 6.3.1.3 Therapeutic effect of DNA...
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Báo cáo y học: " Prospects for control of emerging infectious diseases with plasmid DNA vaccines" doc
Ngày tải lên :
11/08/2014, 08:21
... vector alone [26] In Formulated DNA Vaccines An area of potentially paramount importance for DNA vaccines is formulations and adjuvants Adjuvants are common to most licensed vaccines and are included ... x Figure Manufacturing timelines for DNA vaccines compared to egg-based protein vaccines Manufacturing timelines for DNA vaccines compared to egg-based protein vaccines F/F = fill finish Ability ... proteinbased vaccines for pandemic influenza first therapeutic vaccine approved by the U.S government for the treatment of cancer in animals or humans Approved DNA Vaccines Although DNA vaccines...
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DNA Vaccines
Ngày tải lên :
23/10/2013, 18:15
... Europe Therefore, much effort has been concentrated around developing DNA vaccines against these viral diseases Several parameters have been investigated to optimise the efficacy of the DNA vaccines ... Sciences 1995;772:164e9 Nichols WW Plasmid DNA vaccines: assay for integration [89] Minor PD Regulatory issues in the use of DNA vaccines Aninto host genomic DNA Developments in Biologicals 2000; ... affiliate of Novartis), was cleared for marketing by the Canadian Food Inspection Agency 5 DNA vaccine in fish Table An overview of studies performed with DNA vaccines encoding viral or bacterial...
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Tài liệu Vaccines for preventing influenza in the elderly (Review) docx
Ngày tải lên :
13/02/2014, 18:20
... Influenzavirus B/ (flu or influenza*).tw or/3-6 Vaccines/ vaccines, attenuated/ or vaccines, inactivated/ or exp vaccines, subunit/ or exp vaccines, synthetic/ or viral vaccines/ 10 exp Immunization/ 11 (vaccin* ... comprehensive assessment of the effects of influenza vaccines in the elderly The current pandemic has caused a heightened interest in influenza vaccines and Vaccines for preventing influenza in the elderly (Review) ... with no intervention We also considered new, as yet unlicensed, types of vaccines (for example, live attenuated and DNA vaccines) Vaccination of staff in order to protect patients and residents...
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dna vaccines, methods and protocols
Ngày tải lên :
10/04/2014, 23:12
... Adjuvants for DNA vaccines, in DNA Vaccines: Methods and Protocols, Humana, Totowa, NJ, pp 185–196 13 McCluskie, M J and Davis, H L (1997) Mucosal immunization with DNA vaccines, in DNA Vaccines: ... non-lyophilized control DNA 1.6 In Vivo Bioassays of the Reconstituted Lyophilized DNA Formulation A panel of lyophilized VR1223 plasmid DNA formulations were evaluated for luciferase expression ... Check the DNA received by the Qiagen DNA Production Facility for large-scale manufacturing for its identity first (size, restriction pattern, sequence), and if it is satisfactory, release it for further...
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Báo cáo sinh học: " Buffy coat specimens remain viable as a DNA source for highly multiplexed genome-wide genetic tests after long term storage" pptx
Ngày tải lên :
18/06/2014, 19:20
... Coordinating Center for GWA genotyping before DNA isolation results were available After DNA isolation of these were found to have total DNA yield < 50 ug To conserve these for future analysis, ... total DNA yield (1.07 ug, 4.00 ug, 5.22 ug) and the need to preserve the DNA for future disease genetics studies The TaqMan genotyping assay is a QC test of the suitability of the isolated DNA for ... at 3000 g for versus mins; centrifuges for mins versus mins at 3000 g during initial DNA precipitation step; and during DNA wash, uses 12 ml 70% ethanol versus 10 ml, and centrifuges for versus...
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Báo cáo sinh học: " Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases" doc
Ngày tải lên :
18/06/2014, 22:20
... (Fomivirsen) Affinitac (ISIS 3521) Genasense Alicaforsen (ISIS 2302) ISIS Pharmaceuticals ISIS Genta ISIS CMV IE2 PKC-α Bcl2 ICAM-1 PS DNA PS DNA PS DNA PS DNA Approved in 1998 Phase III Phase III Phase ... ISIS ISIS Methylgene PS DNA PS DNA PS DNA Phase II Phase II Phase II EPI-2010 Asthma PS DNA Phase II GTI 2040 EpiGenesis Pharmaceuticals Lorus Therapeutics Cancer PS DNA Phase II ISIS 104838 ... generation' DNA analogs The 'second generation' ONs contain nucleotides with alkyl modifications at the 2' position of the ribose They are less toxic than PS-DNAs and have a slightly enhanced affinity DNA...
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báo cáo hóa học:" Buffy coat specimens remain viable as a DNA source for highly multiplexed genome-wide genetic tests after long term storage" doc
Ngày tải lên :
20/06/2014, 04:20
... Coordinating Center for GWA genotyping before DNA isolation results were available After DNA isolation of these were found to have total DNA yield < 50 ug To conserve these for future analysis, ... total DNA yield (1.07 ug, 4.00 ug, 5.22 ug) and the need to preserve the DNA for future disease genetics studies The TaqMan genotyping assay is a QC test of the suitability of the isolated DNA for ... at 3000 g for versus mins; centrifuges for mins versus mins at 3000 g during initial DNA precipitation step; and during DNA wash, uses 12 ml 70% ethanol versus 10 ml, and centrifuges for versus...
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báo cáo hóa học:" Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases" pdf
Ngày tải lên :
20/06/2014, 04:20
... (Fomivirsen) Affinitac (ISIS 3521) Genasense Alicaforsen (ISIS 2302) ISIS Pharmaceuticals ISIS Genta ISIS CMV IE2 PKC-α Bcl2 ICAM-1 PS DNA PS DNA PS DNA PS DNA Approved in 1998 Phase III Phase III Phase ... ISIS ISIS Methylgene PS DNA PS DNA PS DNA Phase II Phase II Phase II EPI-2010 Asthma PS DNA Phase II GTI 2040 EpiGenesis Pharmaceuticals Lorus Therapeutics Cancer PS DNA Phase II ISIS 104838 ... generation' DNA analogs The 'second generation' ONs contain nucleotides with alkyl modifications at the 2' position of the ribose They are less toxic than PS-DNAs and have a slightly enhanced affinity DNA...
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Multi wall carbon nanotubes (MWCNTs) doped polypyrrole DNA biosensor for label free detection of genetically modified organisms by QCM and EIS
Ngày tải lên :
02/07/2014, 14:14
... targets for GMO screening in DNA detection approach (this approach is more often used than the second one of protein detection because DNA stability is much higher than that of proteins and therefore ... therefore DNA detection can be used for both raw and processed materials while protein detection is suitable only for raw materials) On the need of fast and sensitive analytical methods for GMO ... Film formation and morphology Normally, sensitivity and reproductibility of DNA sensors are determined by the surface chemistry of the recognition interface, therefore immobilization of DNA probe...
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DNA Vaccines
Ngày tải lên :
15/07/2014, 17:00
... what are now known as DNA Vaccines How is a DNA Vaccine made? DNA is isolated -The DNA sequence which would code for a specific protein is isolated from the genome of the infectious organism ... technology for creating DNA Vaccines for other infectious organisms could be established World would be a safer, healthier, happier place =) References Delude C, Mirvis K, editors 2000 DNA Vaccines ... wildtype form Creates an immune response that may vary in effectiveness depending on the person 1&3 DNA Vaccines and HIV HIV could be prevented using DNA Vaccines -By isolating the gene for a...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
Ngày tải lên :
07/08/2014, 17:22
... hybridization assay for the detection of CHV These results suggest that ISH assay using digoxigenin-labeled probe, which was optimized in this study, may be recommended for diagnosis of CHV in formalin-fixed ... biological diagnostic methods for the detection of intracellular viral DNAs in paraffin sections J Vet Med Sci 2003, 64, 231-235 73 Kim O, Chae C In situ hybridization for the detection and localization ... was done for hours or overnight at 45oC respectively e -; negative, +; weak positive, ++; moderate positive, +++; strong positive b Fig Overnight hybridization with digoxigenin-labeled DNA probes...
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Báo cáo y học: "Vascular disrupting agent DMXAA enhances the antitumor effects generated by therapeutic HPV DNA vaccines" pdf
Ngày tải lên :
10/08/2014, 05:21
... performed specific vaccines, C57BL/6 mice (5 per group) were vaccinated with CRT/E6 DNA or Sig/E7/L1 vaccinia virus or PADRE DNA vaccine via gene gun delivery and treated with DMXAA at days before ... provided by the manufacturer Vaccines The generation of HPV-16 E7-expressing plasmid (pcDNA3-CRT/E7), E6-expressing plasmid (pcDNA3CRT/E6) [8], PADRE-expressing plasmid (pcDNA3- Bio-Plex cytokine ... using the formula 3.14 × [largest diameter × (perpendicular diameter)2]/6 Vaccination Preparation of DNA- coated gold particles and gene gun particle-mediated DNA vaccination was performed using...
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báo cáo khoa học: " Human genomics and preparedness for infectious threats" ppsx
Ngày tải lên :
11/08/2014, 12:20
... for novel associations with infectious diseases, including tuberculosis, malaria, and HIV [21-24] Preparedness for research Public health efforts to control and prevent infectious diseases are based ... development of preventive vaccines; however, novel diagnostic tests developed for clinical use may also prove useful for epidemic monitoring and triage during out reaks For example, b scientists ... White O, Di Francesco V: National Institute of Allergy and Infectious Diseases bioinformatics resource centers: new assets for pathogen informatics Infect Immun 2007, 75:3212-3219 Dawood FS, Jain...
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báo cáo khoa học: " Medicines and vaccines for the world''''s poorest: Is there any prospect for public-private cooperation?" ppt
Ngày tải lên :
11/08/2014, 18:20
... the margin for the Fortune 500 drug firms has consistently exceeded the margin for all Fortune 500 companies taken together It is possible that the higher profits are compensation for higher ... US, Europe and Japan account for 77% of the market although they account for less than 15% of the global population [3] In contrast, Sub-Saharan Africa that accounts for almost 25% of the global ... costs Therefore, successful drugs can generate high profits in accounting terms as the R&D costs for such drugs have already been expensed in the past Nevertheless, Table shows that profits for the...
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Báo cáo y học: "Early postoperative hyperglycaemia is not a risk factor for infectious complications and prolonged in-hospital stay in patients undergoing oesophagectomy: a retrospective analysis of a prospective trial" ppsx
Ngày tải lên :
12/08/2014, 20:20
... control is relatively time consuming for ICU personnel because of frequent glucose monitoring, and it may be hazardous because of the risk for hypoglycaemia It is therefore important to determine which ... automatically selected for multivariate analysis because it was the main aim of the study to determine their relationship with outcome Logistic regression analysis was used for infectious complications, ... and linear regression analysis was used for length of stay Because of nonparametric distribution, length of stay data were logarithmically transformed before regression analysis Parameters included...
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Báo cáo y học: "DNA signatures for detecting genetic engineering in bacteria" ppt
Ngày tải lên :
14/08/2014, 08:20
... plasmids and chromosomes, respectively may form the basis for future vectors based on conserved functional sequences Results and discussion Vector DNA signatures A total of 3,799 partial and ... synthesized microarray platform An alternative BLAST approach called MCS-only was included for comparison MCS-only uses the multiple cloning sites of vectors exclusively as the source for creating signatures ... our best efforts, we were unable to obtain multiple cloning site annotations for 18% of the completely sequenced vectors, although given the redundancy among vectors, the potential for extracting...
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Báo cáo sinh học: "DNA vaccines: improving expression of antigens" docx
Ngày tải lên :
14/08/2014, 19:22
... number not for citation purposes) Genetic Vaccines and Therapy 2003, expression systems where gene products are only expressed in the critical cell types for DNA vaccination or gene therapy, for example, ... to account for strong CTL responses frequently seen following DNA vaccination [44] It is possible to augment responses to DNA vaccine vectors by incorporating CpG motifs into the DNA backbone ... Gurunathan et al [4]) The approaches outlined above will together allow for the rational and optimised design for DNA vaccines and gene therapy vectors The ability to improve antigen expression...
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Báo cáo sinh học: "DNA vaccines: designing strategies against parasitic infections" potx
Ngày tải lên :
14/08/2014, 19:22
... Attenuated fowlpox virus or DNA MVA DNA Recombinant protein DNA Pox virus DNA Recombinant protein DNA RTS, S/ASOZA Leishmania infantum LACK DNA Recombinant vaccinia virus DNA Recombinant vaccinia ... against challenge compared to DNA alone DNA vaccines were tested against S masoni challenge in mice Boosting with MVA for the same genes had no increased effect expect for mutated GP antigen were ... best strategy for an anti-pathology vaccine is immune deviation Pathology in schistosomiasis is associated with egg-induced granuloma formation for which there is evidence for a role for Th2 cytokines...
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Báo cáo sinh học: "Comparison of different delivery systems of DNA vaccination for the induction of protection against tuberculosis in mice and guinea pigs" pps
Ngày tải lên :
14/08/2014, 19:22
... have been employed for generation and evaluation of new TB vaccines Recombinant BCG strains, DNA- based vaccines, live attenuated M tuberculosis vaccines and subunit vaccines formulated with novel ... animals vaccinated and challenged with M tuberculosis Formulationsa Miceb Guine-pigsb No vaccination BCG DNAv (naked DNA) DNAhsp65 (naked DNA) Me-DNAhsp65/TDM Me-Prime/boost 74 ± 18 ± 5* 72 ± 38 ± ... Encapsulation efficiency varied from 30 to 50% for DNA and around 70% for protein Table shows the amount of entrapped DNA and protein in the different formulations In this work, particles were designed...
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