dna repair and genomic stability in drosophila

... participates in alignment-based gap filling for nonhomologous DNA end joining [33] Therefore, Pol k may participate not only in BER but also in DNA doublestrand break repair and meiosis, and Pol b and ... suggesting that the DNA polymerase activity of this enzyme may be important for plant cells On the other hand, the N-terminal BRCT domain proposed to mediate protein–protein interactions involved in ... NaCl in buffer A The eluted OsPol k protein was dialyzed against buffer A and used in the subsequent experiments Standard DNA polymerase assay A DNA polymerase assay was performed as described in...

Ngày tải lên: 07/03/2014, 15:20

... estimate DNA repair Nevertheless, the underlying mechanisms affecting cells embedded in agarose during DNA repair in PFGE methodology are still not well understood To better understand spontaneous DNA ... be inhibited by caspase inhibitors), and 3) reducing the interference of spontaneous breakage improves our ability to estimate DNA repair and to determine apoptosis intensity Methods Cell lines ... radiation induced DNA breakage, which is attributable to rejoining, continued diminishing (Figure 5) The blockade of apoptosis thus allowed us to efficiently determine rejoining without the inference...

Ngày tải lên: 09/08/2014, 09:20

... data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3-kinase pathways including thrombin, dexamethasone, angiotensin II and prostaglandins stimulate ... barbed-end actin and determine the level and distribution of F-actin [34] Therefore, it is tempting to speculate that interactions between monomeric G-actin and actin-binding proteins are a potential ... for 30 and then fixed, permeabilized and stained for F-actin with rhodamine-conjugated phalloidin The effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the...

Ngày tải lên: 19/02/2014, 16:20

... glycine, l-alanine, l-proline, l-lysine, l-serine, b-alanine, taurine, sarcosine, dimethylglycine, glycine betaine, trimethylamine N-oxide, and cytidine 2¢-3¢ cyclic monophosphate were also obtained ... (glycine, alanine, proline, serine, lysine, b-alanine and taurine) that have no significant effects on both DGD° and kcat (b) Class II represents methylamines (sarco- Functional stability and activity ... & Fink AL (2001) Trimethylamine-Noxide-induced folding of a-synuclein FEBS Lett 509, 31–35 Meng F, Park Y & Zhou H (2001) Role of proline, glycerol, and heparin as protein folding aids during...

Ngày tải lên: 07/03/2014, 00:20

... contamination of reagents or equipment with amplicon DNA Uracil -DNA glycosylase (UNG) is a DNA repair enzyme that will cleave uracil-containing DNA while leaving the natural, thymine-containing DNA ... containing UNG and incubated at 30°C for 20 slightly greater than the standards that did not contain UNG and were not incubated prior to RT-PCR The yintercept value for the reactions not containing ... contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact The precise, reproducible quantification of realtime RT-PCR provides a rapid method to assess and optimize...

Ngày tải lên: 19/06/2014, 08:20

... was mounted in an Instron servohydraulic testing machine (Model 1321, Instron, Canton, Massachusetts) so the long axis of the femoral stem, the rotational axis of the Instron machine and the femoral ... fixation and biologic ingrowth has not been conclusively determined Our understanding of bypass fixation in the periprosthetic femur with deficient bone stock has come largely from studies involving ... the proximal metaphysis, incorporating proximal fixation into the biomechanical testing [15] In another biomechanical study, authors reported inferior torsional stability in isolated distal diaphyseal...

Ngày tải lên: 20/06/2014, 00:20

... contamination of reagents or equipment with amplicon DNA Uracil -DNA glycosylase (UNG) is a DNA repair enzyme that will cleave uracil-containing DNA while leaving the natural, thymine-containing DNA ... containing UNG and incubated at 30°C for 20 slightly greater than the standards that did not contain UNG and were not incubated prior to RT-PCR The yintercept value for the reactions not containing ... contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact The precise, reproducible quantification of realtime RT-PCR provides a rapid method to assess and optimize...

Ngày tải lên: 20/06/2014, 04:20

... recruitment of DNA repair and chromatin remodelling proteins to DNA damage sites Three types of PAR-binding motifs have been identified: the PBZ (PAR-binding zinc finger), the macrodomain and an amino acid ... Pigmentosum 145 Carolina Quayle, Carlos Frederico Martins Menck and Keronninn Moreno Lima-Bessa Part DNA Repair and Cancer 175 Chapter DNA Damage, Repair and Misrepair in Cancer and in Cancer Therapy ... signalling are ATR (ATM and Rad3 related) and DNA- PKcs ATM and DNA- PKcs are primarily involved in the signalling of DSBs and ATR is mainly required in response to stalled DNA replication forks The interaction...

Ngày tải lên: 27/06/2014, 19:20

... recruitment of DNA repair and chromatin remodelling proteins to DNA damage sites Three types of PAR-binding motifs have been identified: the PBZ (PAR-binding zinc finger), the macrodomain and an amino acid ... Pigmentosum 145 Carolina Quayle, Carlos Frederico Martins Menck and Keronninn Moreno Lima-Bessa Part DNA Repair and Cancer 175 Chapter DNA Damage, Repair and Misrepair in Cancer and in Cancer Therapy ... signalling are ATR (ATM and Rad3 related) and DNA- PKcs ATM and DNA- PKcs are primarily involved in the signalling of DSBs and ATR is mainly required in response to stalled DNA replication forks The interaction...

Ngày tải lên: 28/06/2014, 03:20

... iodide and mithramycin stainings Results are listed in table II In F sylvatica, the GC content was 40.0 ± 0.2 %; in the tortuosa variety, 39.8 ± 0.2 % These values are not significantly different and ... error in cytophotometric determination of nuclear DNA content in plants, Plant Syst Evol 158 (1988) 87-96 advice [17] Laurie D.A., Bennett M.D., Nuclear DNA content in the genera Zea and Sorghum Intergeneric, ... is extremely rapid and convenient: it allows determinations of nuclear DNA content [13] and of AT/GC base composition in a genome [15] Favre and Brown [ 12] developed a fast and simple flow cytometry...

Ngày tải lên: 08/08/2014, 14:21

... on fluctuating genomic DNA contents can be attributed to unrecognized stoichiometric errors induced by plant tannins" Correspondingly, microdensitometry tends to underevaluate the DNA content ... Heterochromatin study demonstrating the non-linearity of fluorometry useful for calculating genomic base composition Cytometry 14, 618-626 Greilhuber J (1988) ’Self-tanning’ a new and important ... base-specific dye and applying the fifth root relationship of Godelle et al (1993): RESULTS AND DISCUSSION Results are given in tableI They show a relatively uniform nuclear DNA content and base composition...

Ngày tải lên: 08/08/2014, 18:21

... such as wing and thorax length was first investigated over two environments (Scheiner and Lyman, 1989, 1991; Scheiner et al, 1991; Weber and Scheiner, 1992; Scheiner, 1993a) and a linear model ... wing and thorax; the Wing—thorax wing/thorax ratio correlation The wing-thorax correlation was investigated at the individual (= within lines) and at the line (= between line means) levels ... positively correlated in both species and, among lines, thorax and wing TMS values were also correlated in D melanogaster (David et al, 1994) Interestingly in D melanogaster, calculating the TMS values...

Ngày tải lên: 09/08/2014, 18:22

... population in cages with overlapping generations Flies were maintained on a medium of yeast, sugar, cornmeal and agar at 25°C Experiments were initiated in the spring of 1994 Heat resistance and induced ... wing size, as also shown in the same and other Drosophila species by the authors quoted in the introduction to this work (Morrison and Milkman, 1978; Stephanou and Alahiotis, 1983; Quintana and ... to a general hardiness or weakness of some lines that is independent of the shock response Inbreeding is expected within isofemale lines and uncontrolled genetic drift or inbreeding may lead to...

Ngày tải lên: 09/08/2014, 18:22

... rainfall in Asia that was not apparent in Europe or North America In the Wandin North population, Gpdh-F frequency was independent of rainfall Factors affecting genetic variation patterns within ... 2,000 in the vicinity of the vineyard buildings) These numbers are more similar to those found in an equivalent area of the orchard In general, Drosophila tend to remain in the , ALLACE vicinity ... Variation Within the Orchard Tpi-F frequency fluctuated seasonally, characterised by an increase in the F in autumn and winter months (fig 2) Total monthly rainfall, mean daily maximum and minimum temperature...

Ngày tải lên: 09/08/2014, 22:23

... the intersection between the observed and the expected distance distribution The intersection can be interpreted as the point separating the intra-cluster from the inter-cluster distances In this ... is defined by its start and end coordinates and the number of k-mers or genomic elements it contain Calculation of the statistical significance for each cluster by means of the negative binomial ... as failures Finally, p is the success probability, i.e the probability to find a target k-mer or genomic element within the DNA sequence Note that in the above equation we use (n-1) instead of...

Ngày tải lên: 12/08/2014, 17:20

... within a treatment and having adult offspring in brood were included in the analysis Standard errors were calculated according to Meyer (1985, 1986) In order to estimate genotype-environment interactions ... reduction in both female and male offspring and the higher sterility in broods 4-5 indicate that germinal selection is an important mechanism in eliminating deleterious mutations Vogel and Zijlstra ... progeny, indicating that the induced mutations are mainly recessive Almost normal sex proportion in broods and after EMS treatment suggests that efficient DNA repair processes exist in the meiotic and...

Ngày tải lên: 14/08/2014, 19:22

... 1983) and the water balance is maintained by water ingestion (Fairbanks and Burch, 1970; Arlian and Eckstrand, 1975) In addition the availability of resources is variable among localities and seasons, ... manihot peelings in Congo In each case, wild collected females were used to initiate isofemale lines These lines were kept in the laboratory for a number of generations ranging between and 12 before ... problem of overwintering during the coldest months of the year Samples were collected using either banana fermenting baits or by sweeping over natural breeding sites, ie Opu!tia fruits in Tunisia...

Ngày tải lên: 14/08/2014, 20:20

... cross A) and an inducer strain with regard to the IR system The Gruta strain (captured in Argentina in 1950) is an MR strain, as are most old laboratory strains The Canton-S and Harwich strains are ... specially in the dysgenic lines (table II) Relaxation of selection resulted in decreasing the mean body weight of the heavy lines and increasing that of the light lines, for both types of original ... lines were found to be inducer lines in the IR system = Molecula.r analysis In situ hybridization was performed in order to detect transposition, by comparing the sites in the selected lines and...

Ngày tải lên: 14/08/2014, 20:20

... of insulin and wingless signaling pathways in Drosophila S2 cells Figure Activation of insulin and wingless signaling pathways in Drosophila S2 cells (a) Schematic representation of the insulin ... upon activation of the insulin and wingless pathways in S2 cells Binding of insulin-like peptides to the insulin receptor in Drosophila cells leads to the activation of dPI3 kinase, which Genome ... of uridine-rich small nuclear ribonucleoprotein particles and more than 100 additional proteins [21,22] Splicing regulatory factors, including members of the Serine and Arginine-rich (SR) and heterogeneous...

Ngày tải lên: 14/08/2014, 21:20