dna methylation changes in prostate cancer

... Activation in Prostate Cancer 589 Luis A Espinoza VII VIII Contents Section Cell Adhesion Proteins in Prostate Cancer 617 Chapter 25 Integrins in Prostate Cancer Invasion and Metastasis 619 Paulynn Chin ... of all prostate cancer cases The risk of getting prostate cancer increases nearly exponentially with increasing age This makes prostate cancer one of the most distinctive cancers in aging popu‐ ... of Prostate Cancer 331 Chapter 14 Stem Cells and Prostate Cancer 333 Vildan Bozok Çetintaş, Bur in Tezcanlı Kaymaz and Buket Kosova Chapter 15 Salinomycin-Induced Apoptosis in Human Prostate Cancer...

Ngày tải lên: 22/03/2014, 22:20

... produced by LNCaP -prostate cancer cell line Prostate cancer cell lines (LNCaP and DU145), as indicated, were maintained in culture as a source of positive-control prostate cancer exosomes (for ... of urine (for at least 18 hours at 37°C) (Figure 6c) Discussion We present the findings of a pilot study, investigating urinary exosomes in prostate cancer patients We had two main aims in the ... loading Bands for prostate- derived proteins PSA or PSMA were evident in patients (p1, p7, p8, p9, p10), indicating that at least some of the exosomes present in the urine were of prostate origin...

Ngày tải lên: 18/06/2014, 15:20

... Against HLA-A24-positive AMACR-positive Prostate Cancer Cell Lines To confirm that CTLs induced with AMACR peptides could exert cytotoxicity against AMACR-expressing prostate cancer cell lines in ... immunohistochemical staining In contrast, PSA was stained in both prostate cancer tissue and non-cancerous tissue (Figure 2C) These data indicated that AMACR had a mostly cancer- specific expression ... Immunostaining of prostate cancer tissue with antibodies against AMACR and PSA Immunostaining of prostate cancer tissue with antibodies against AMACR and PSA Surgically resected prostate cancer tissue...

Ngày tải lên: 18/06/2014, 15:20

... replicate in these cells Findings This report builds on and challenges the published data on the absence of A3G protein in prostate epithelial cells lines We demonstrate the presence of A3G in prostate ... cell lines specifically in LNCaP cells [3,18] In addition, the prostate cancer cell line 22Rv1 has been shown to be chronically infected with XMRV and produces highly infectious virus [19] Since ... media containing FBS were added After 1–2 weeks, XMRV infection was confirmed by detecting XMRV p30 protein by using goat polyclonal anti-Rauscher MLV p30 Gag in uninfected (−) and XMRV infected...

Ngày tải lên: 18/06/2014, 18:20

... MDA-7/IL-24 in prostate cancer cells We have stained MDA-7 in prostate cancer cells Shown in Figure 5A, PC-3 wild type cells stained for MDA-7, mostly in the cytosolic region and perinucleus areas ... Biotechnologies, Inc (Santa Cruz, CA, USA), Akt inhibitor, SIS3 inhibitor, PLC-gamma inhibitor, JNK inhibitor, JAK inhibitor, MET inhibitor, Wortmannin, and Wiskostatin were from Calbiochem (Nottingham, ... TGase-4 is linked to the invasiveness of prostate cancer cells [12] and participates in the regulation of the interactions between prostate cancer cells and endothelial cells, the later involving the...

Ngày tải lên: 18/06/2014, 19:20

... MDA-7/IL-24 in prostate cancer cells We have stained MDA-7 in prostate cancer cells Shown in Figure 5A, PC-3 wild type cells stained for MDA-7, mostly in the cytosolic region and perinucleus areas ... Biotechnologies, Inc (Santa Cruz, CA, USA), Akt inhibitor, SIS3 inhibitor, PLC-gamma inhibitor, JNK inhibitor, JAK inhibitor, MET inhibitor, Wortmannin, and Wiskostatin were from Calbiochem (Nottingham, ... TGase-4 is linked to the invasiveness of prostate cancer cells [12] and participates in the regulation of the interactions between prostate cancer cells and endothelial cells, the later involving the...

Ngày tải lên: 20/06/2014, 03:20

... value concerning the primary detection and the staging of prostate cancer [27] In principle, signal-generation is based on an increased choline metabolism in prostate cancer leading to an increased ... planning in prostate cancer Q J Nucl Med Mol Imag 2009, 53(2):245-268 28 Reske SN, Blumstein NM, Glatting G: [C-11]choline PET/CT imaging in occult local relapse of prostate cancer after radical prostatectomy ... [39] In such a geometrical setting it is defined as: TCP  e  n i SFi i SF i is the surviving fraction within the single subvolume with the running index i (ranging from to m = V/vi) Using the...

Ngày tải lên: 09/08/2014, 08:22

... inhibitors of the PI3K/Akt pathway in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate cancer cell lines Up to now our data revealed that ErPC3 is a potent inhibitor ... lost or inactivated in a variety of solid human tumors, including prostate cancer Increased levels of PIP3 trigger the recruitment of phosphatidylinositide-dependent serine/threonine kinase (PDK1) ... serine 473 but phosphorylation on serine 473 seems to be independent of PDK1 Although several kinases, such as integrinlinked kinase, DNA- dependent protein kinase (DNAPK), and the mTOR/Rictor-complex...

Ngày tải lên: 09/08/2014, 09:20

... needing knowledge on the combination of underlying biological information The presented results were obtained in a preclinical study in prostate cancer xenografts, and suggest that the combination ... training, testing and validating the network showed curve flattening after a few training epochs, this indicated that no overtraining occurred However, if such a model is to be applied on clinical ... cannula with saline-diluted Gd-DTPA (Magnevist ® , Schering, Berlin, Germany) was inserted into the tail vein Dynamic T1-weighted (T1W) imaging was acquired by performing 20 minutes of dynamic...

Ngày tải lên: 09/08/2014, 09:20

... becoming one of the most significant issues in treatment decision-making, in general, and more so in prostate cancer [1] Thus late rectal and urinary damage became a major concern in prostate cancer; ... participating in the non-self recognition of prostate cancer cells Thus we can hypothesize, according to results of Hurwitz et al [12] that the enhanced immune function, involving resting and terminally ... with a Becton Dickinson LSRII instrument (BD Biosciences) We included single-stain controls for further offline compensation Measurement and subsequent analysis was performed using FACSDiva (BD...

Ngày tải lên: 09/08/2014, 09:20

... FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein expression ... Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival signal transduction pathways Int ... anti -prostate cancer activity in vitro and in vivo Clin Cancer Res 2004, 10:5282-5292 Ouchi H, Ishiguro H, Ikeda N, Hori M, Kubota Y, Uemura H: Genistein induces cell growth inhibition in prostate...

Ngày tải lên: 09/08/2014, 09:22

... Kemmerling L, Born EJ, Mini R, Greiner RH, Aebersold DM: Daily organ tracking in intensitymodulated radiotherapy of prostate cancer using an electronic portal imaging device with a dose saving acquisition ... summarized in Table Incidence of single symptoms (dysuria, incontinence, retention, frequency/urgency, hematuria) as well as the highest toxicity occurred in a given patient concluding all single ... rectum and bulb of the penis using MRI delineation for radiotherapy of the prostate Int J Radiat Oncol Biol Phys 2003, 57:1269-79 Sobin LH, Wittekind CH: The prostate In TNM Classification of Malignant...

Ngày tải lên: 09/08/2014, 09:22

... performed on-line by repositioning the target according to the internal markers, moving the treatment couch manually To calculate the minimum required margin width around the clinical target ... 90%inthe median values (lines percentile 75% values10%variability, prostate line), format, Measured deviations in and Measured deviations in LR, SI and AP directions in a box plot format, showing ... the interobserver variability is low Therefore implanted markers and EPID based methods are used for targeting in radiotherapy of prostate cancer with increasing frequency Our results provide information...

Ngày tải lên: 09/08/2014, 09:22

... participated in study design, data interpretation, manuscript drafting and revising for intellectual content SZ participated in study design, data interpretation and manuscript revising for intellectual ... Bolognesi A, Cattaneo GM, Calandrino R: Intraand inter-observer variability in contouring prostate and seminal vesicles: implications for conformal treatment planning Radiother Oncol 1998, 47:285-292 ... Shinohara N, Harabayashi T, Miyasaka K: Threedimensional intrafractional movement of prostate measured during real-time tumor-tracking radiotherapy in supine and prone treatment positions Int...

Ngày tải lên: 09/08/2014, 10:21

... somatostatin and Octreotide in prostate cancer cell lines To examine the regulatory effects of somatostatin and Octreotide on XAF1 mRNA and protein expression, prostate cancer cell lines (LNCaP, ... cell lines We found that somatostatin and Octreotide up-regulated XAF1 mRNA and protein expression in prostate cancer cell lines The enhanced XAF1 expression by somatostatin indicates a promising ... [23] In the current study, we examined XAF1 mRNA and protein expression in four cell lines, and determined regulatory effects of somatostatin and Octreotide on XAF1 expression in prostate cancer...

Ngày tải lên: 10/08/2014, 10:20

... HM: IFNγ induction of p21waf1 in prostate cancer cells: Role in cell cycle, alteration of phenotype and invasive potential Int J Cancer 1998, 77:138-145 Sica G, Iacopino F, Recchia F: Interferon ... chemosensitizing effect of PDF has been postulated on conventional anticancer drugs being currently used [13] http://www.jhoonline.org/content/1/1/25 Accordingly, we are interested in investigating whether ... protein kinase called double-stranded DNA- dependent protein kinase (DNA- PK), which requires small double-stranded DNA for its activation [20] DNA- PK is also believed to play an important role in...

Ngày tải lên: 10/08/2014, 22:20

... viral DNA detection and RNASEL R462Q in our clinical samples (Table 5) Phylogenetic analyses of MLV-like sequences in prostate tissue DNA XMRV has been PCR amplified from prostate cancer samples in ... retroelements (IAPs) Together with our data, these findings highlight the difficulty of avoiding DNA contamination in clinical samples and the risk of testing contaminated samples as XMRV-positive by sensitive ... contaminating mouse genome originated from a PCR kit Since we could amplify the viral sequences from multiple aliquoted DNA samples, they appeared to be contaminated before or during the DNA isolation...

Ngày tải lên: 13/08/2014, 01:20

... targeted therapeutics for prostate cancer candidate predictor of efficacy in both prostate and breast cancer Finally, the observed sensitivity to dasatinib of prostate cancer cell lines with expression ... heterogeneity of prostate tumors and the difficulty in obtaining biopsies from prostate cancer patients It remains to be determined whether the molecular subtypes of prostate cancer identified ... JS: RNA interference-directed knockdown of urokinase plasminogen activator and urokinase plasminogen activator receptor inhibits prostate cancer cell invasion, survival, and tumorigenicity in vivo...

Ngày tải lên: 14/08/2014, 08:20