dna extraction from keratin and chitin

... pH Drying Chitin Figure 3.39: Proposed procedue for recovering chitin and protein from heads of white leg shrimp Chitin Figure 3.40: Proposed procedue for recovering chitin and protein from shells ... of chitin and chitosan produced according to the proposed procedures Chitin and chitosan produced from shrimp heads and shells according to the proposed procedures in Figure 3.39, Figure 3.40 and ... minerals and chitin of head and shell parts were 44,39±1,84 and 26,11±1,15%; 26,66±1,63 and 25,61±1,79%; 11,40±1,69 and 27,37±1,16%, respectively; The content of amino acid was approximate of 50% and...

Ngày tải lên: 15/08/2015, 22:14

... against the Watson strand from the C/T reference and the Crick strand from the G/A reference (MethylC-Seq) or against both strands of the two reference genomes 2.3 Post-processing and output Once the ... knowledge of the distribution and location of CG can be utilized to understand the biological DNA sequences are defined and classified as CpG islands if , (a) length of that DNA sequence >200 bp, (b) ... of its double strands methylated DNA Methylation – From GenomicsWill-be-set-by-IN-TECH to Technology The above considerations make a compelling case to model and understand the DNA methylation...

Ngày tải lên: 08/03/2014, 19:20

... extraction: Techniques and challenges In M T Pazienza, editor, Information Extraction: a multidisciplinary approach to an emerging technology Springer Verlag Adam Kilgarriff 1997 Foreground and ... needs First there is no clear way of extracting from them the mapping between the FL and the ontology; this is mainly due to a lack of information and 226 cannot in general be solved; generic lexica ... ones; moreover we can also inspect its coordinated terms and assign the same label to of the 33 coordinate terms (and then to their direct and indirect hyponyms) Marking is equivalent to assigning...

Ngày tải lên: 08/03/2014, 21:20

... 1995; Fung and McKeown, 1997) and compositional analysis (Daille and Morin, 2008) Symbolic, statistical, and hybrid techniques have been implemented for bilingual lexicon extraction (Morin and Prochasson, ... scenarios defined in the toolkit: “parallel data mining from comparable corpora” and “named entity/terminology extraction and mapping from comparable corpora” The next section provides a general ... workflow of the toolkit allows NE and terminology extraction and mapping Starting with named entity recognition, the toolkit features the first NER systems for Latvian and Lithuanian (Pinnis, 2012)...

Ngày tải lên: 16/03/2014, 20:20

... between bandwidth and largeur de bande can be obtained through the edge linking these two units (type 1), or through two edges, one from bandwidth to largeur de bande., and one from bandwidth ... two edges from bandwidth to largeur and bande (type 3), secondly, there may be conflicts between connections: in figure both largeur de bande and tdldcommunications are linked to bandwidth even ... probability of observing the alignment a together with the English and French sentences, es and f~, and (a,e,f)~ -1 is a shorthand for • from the English words (including the empty one) to the French...

Ngày tải lên: 17/03/2014, 07:20

... of EACL '99 and DNA entities was too small to train with As part of the overall project we are creating an expert-tagged corpus of MEDLINE abstracts and full papers for training and testing our ... extraction We are using information extraction methods to automatically extract named entity properties, events and other domain-specific concepts from MEDLINE abstracts and full texts One part of this ... experts and the information extraction programs Our interface provides a link to the information extraction programs as well as clickable links to aid in querying for related information from publically...

Ngày tải lên: 17/03/2014, 23:20

... estimates of (a1 , a2 ) from fz1 (n) and estimates of (b1 , b2 ) from fz2 (n) For comparison purposes, the same noisy signal y(n) is also put through the HAF algorithm [7] From this algorithm, we ... (or part of it), then the next one, and so on until the last one Normally, the overall energy in the TFD becomes smaller and smaller after each extraction and after the last component has been ... d’Etat in electronics from the Ecole Nationale Polytechnique d’Alger (ENPA) in 1985 and the M.S degree in control systems from the University of Colorado, Boulder, USA, in 1988 From 1989 to 1995,...

Ngày tải lên: 23/06/2014, 01:20

... provided DNA good quality by a quick method of extraction from tree species which have often been a problem regarding extraction of their DNA DNA yields from a 0.1gsample are sufficient for PCR and ... Acer For oak, the yield of DNA ranged from 200 to 950 μg DNA per g fresh weight per clone and for the three other species the yields ranged from 350 to 950 μg DNA per g fresh weight OD260/OD280 ... availability of DNA in sufficient quantity and of good quality and purity Although several different extraction methods have published for herbaceous plants and trees [1- 4, 8, been 11-13 ]and are even...

Ngày tải lên: 08/08/2014, 14:21

... of clinical samples, culture of C trachomatis and performed DNA extraction, PCR analysis and drafted the manuscript IB and SM performed parts of DNA extraction as well as parts of PCR analysis ... performed immediately after DNA extraction DNA aliquots from serial dilution assays extracted by alkaline lysis, Qiaex gel extraction kit® + CTAB, Qiagen tissue kit® and Qiagen stool kit® were ... positive in the subsequent PCR analysis PCR and LCR analysis Template DNA from SF EB and SF C trachomatis PBMO as prepared by all previously described DNA extraction methods was subjected to PCR...

Ngày tải lên: 12/08/2014, 11:22

... Figure and proviral DNA Model of transcription initiation from non-integrated DNA Model of transcription initiation from non-integrated DNA and proviral DNA (A) viral early transcription from nonintegrated ... Integration Figure grated viral DNA and provirus HIV-1 life cycle and model of transcription from pre-inteHIV-1 life cycle and model of transcription from pre-integrated viral DNA and provirus Following ... HIV DNA exists as three forms, the 1-LTR circle, the 2-LTR circle and the linear DNA The circular forms of retroviral DNA were first demonstrated by Varmus and Guntaka as closed circular DNA...

Ngày tải lên: 13/08/2014, 13:20

... 16, 20, 42–44], and references therein can perform very adequately as well, at a fraction of the cost of labor and consumables Using DNA pools for 52 populations from Europe and 46 from China, we ... both of the major areas of pig domestication and breed diversity MATERIAL AND METHODS 2.1 Sampling and DNA preparation A total of 98 lines from Europe and China were sampled; these are listed in ... Zhang et al [50], and Fang et al [12] DNA was isolated from individual samples using standard phenol-chloroform protocols [32] Pools of DNA were made by adding equal amounts of DNA for each of...

Ngày tải lên: 14/08/2014, 13:22

... other extraction conditions For extraction, the two main methods are applied: Extraction with water solvent steamed in pressure cooker and Soxhlet extraction with organic solvents as acetone and ... Physicochemical method Three extraction methods are used to extract HCA from leaves, fruit rinds of the tree: extraction in pressure cooker, soxhlet extraction, and extraction by microwave energy ... products from Garcinia in an more effective and scientific way For such reasons, we have chosen the research subject “Study on extraction, conversion of hydroxycitric acid from leaves and fruit...

Ngày tải lên: 07/05/2015, 21:00

... (d), the gap SCs and the text SCs are shown in gray The two gap SCs are between ‗o‘ and ‗n‘, and between ‗n‘ and ‗e‘ The remaining SCs are all text SCs 65 Figure 3.2 Text candidate identification ... text and background, and the mean and variance of the distances between these transitions Because this method makes extensive use of heuristic rules and threshold values for analyzing the candidate ... designed several character-level and chain-level features and used Random Forest (Breiman 2001) as classifiers Stroke Width Transform-based methods are fast and are able to handle multi-oriented text...

Ngày tải lên: 12/09/2015, 11:24

... borax Object: Anthocyanin from Basella rubra L 3.5.1 Identification of Borax Anthocyanin extract from fruits was diluted into 20%, 10% and 5% in pH buffer solutions from to and reacted with Di Sodium ... yield was 3.33%.Moreover, the IC50 index from antioxidant activity experiment was µg/mL and lower when compared with ascorbic acid and garlic acid 8.4 µg/mL and 2.6 µg/mL respectively, this result ... antiinflammatory and prevent cardiovascular diseases (Joseph,1999; Pawlowicz, 2000) Thanks to the natural qualities and valuable functions, anthocyanins have been leading scientists and companies to study and...

Ngày tải lên: 06/10/2015, 15:58

... supernatant and in the obtained chitin Results and Discussion The overall process for the preparation of shrimp chitin is given in Figure The raw shrimp shells contain about 20% of chitin and other ... purified chitin, it remains the most commonly used decalcifying agent in both laboratory and industrial scale production of chitin It is generally used at a concentration of M Chang and Tsai12 ... H2O (3) Optimization of Chitin Extraction Figure Kinetics of demineralization: (A) HCl was initially in default, and then, 10 mL of HCl 0.25 M was successively added at 120 and 180 min; (B) HCl...

Ngày tải lên: 25/08/2016, 11:28

... sequences using DWT and neural network DWT decomposes one original image into four sub-bands The transformed image includes one average component sub-band and three detail component sub-bands Each detail ... operation and the final resulted 2-D Haar DWT is shown in Figure 3(c) 2-D Haar DWT decomposes a gray-level image into one average component sub-band and three detail component sub-bands From these ... regions from the detail component sub-bands in Figure In next subsection, a neural network is employed to learn the features of candidate text regions obtained from those detail component sub-bands...

Ngày tải lên: 05/11/2012, 14:51

... Kato, H Hase, and T Watanabe Character Extraction from Noisy Background for an Automatic Reference System ICDAR1999 pp.143-146 [4] P Matti and O Okun Edge-Based Method for Text Detection from Complex ... positions (average color) and the standard errors of three parts: the whole histogram, left part of histogram and right part of histogram Besides the features extracted from the color histogram ... text from name cards: 1) Variation of background color and text color (varying from line to line); 2) Complex graphical foregrounds like logos or pictures; 3) Large variation of the text sizes and...

Ngày tải lên: 05/11/2012, 14:54

... perfringens isolates from cattle and pigs with and without Prevalence of C perfringens isolated from fecal samples were shown in Table diarrhea were 85.33%, 86.67%, 82.89%, and 66.67%, respectively ... perfringens from healthy and diseased sheep by multiplex PCR Vet Med - Czech 50, 439-442 631 Genotyping of Clostridium perfringens isolated from cattle and pigs with diarrhea in Hanoi and surrounding ... Frey, M.R Popoff, and J.F van den Bosch (1999) Detection of the β2 toxin gene of Clostridium perfringens in diarrhoeic piglets in The Netherlands and Switzerland FEMS Immunology and Medical Microbiology...

Ngày tải lên: 29/08/2013, 08:14

... F Xu, H Matsuda, and M Yoshikawa (2002) Structures and radical scavenging activities of novel norstilbene dimer, longusone A, and new stilbene dimers, longusols A, B, and C, from Egyptian herbal ... vegetation used in artificial floating islands releases anti-cyanobacterial allelochemicals and to identify such fascinating compounds MATERIALS AND METHODS Plants and cyanobacterium Commercially obtained ... medium (Watanabe and Satake, 1991) in a test tube, and after that M aeruginosa - 56 - Journal of Water and Environment Technology, Vol 6, No.1, 2008 was inoculated (1 × 104 cells/ml), and incubated...

Ngày tải lên: 05/09/2013, 09:38

... river from various contamination sources settle on river and estuarine sediment, and that the sediments may protect the bacteria from rapid decay as suggested by Gerba and McLeod (1976) and Anderson ... (Wait and Sobsey, 1983; Lewis et al., 1985) Moreover, it is reported that humic substances which are extracted from soil and sediment inhibit nucleic acids extraction (Zhou et al., 1996) and Taq DNA ... because of high proportion of fine particles such as silt and clay and presence of humic substances Direct DNA extraction method using DNA extraction kit for soil (ISOIL for Beads Beating) showed...

Ngày tải lên: 05/09/2013, 10:15