dna damage signaling downstream of atm

... Bitomsky & T G Hofmann Fig Regulation of DNA damage- induced cell death by p53 and HIPK2 Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated protein kinases ATM and ... extent of DNA damage, which in turn determines whether DNA lesions can, or cannot, be repaired As unrepaired DNA damage is characterized by constant activity of the DNA damage checkpoint kinase ATM ... activation of ATM in response to UV treatment or replication fork stalling EMBO J 25, 5775–5782 10 Rouse J & Jackson SP (2002) Interfaces between the detection, signaling, and repair of DNA damage...

Ngày tải lên: 16/03/2014, 00:20

... cause DNA damage, thus escaping from the surveillance of DNA damage response network Examination of a variety of oncogenes shows that activation of the majority of oncogenes could evoke DNA damage ... candidates in DNA damage signaling pathway that play a role in cell cycle checkpoint, DNA damage repair or apoptosis (Matsuoka, Ballif et al 2007) The importance of ATM and ATR in DNA damage signaling ... 1.3 ATM and ATR kinases 1.4 DNA damage response 10 1.5 1.4.1 DNA damage sensing 10 1.4.2 DNA damage mediating - ATM and ATR activation 11 1.4.3 DNA damage...

Ngày tải lên: 12/10/2015, 17:35

... cause DNA damage, thus escaping from the surveillance of DNA damage response network Examination of a variety of oncogenes shows that activation of the majority of oncogenes could evoke DNA damage ... candidates in DNA damage signaling pathway that play a role in cell cycle checkpoint, DNA damage repair or apoptosis (Matsuoka, Ballif et al 2007) The importance of ATM and ATR in DNA damage signaling ... 1.3 ATM and ATR kinases 1.4 DNA damage response 10 1.5 1.4.1 DNA damage sensing 10 1.4.2 DNA damage mediating - ATM and ATR activation 11 1.4.3 DNA damage...

Ngày tải lên: 26/11/2015, 22:49

... sensing of DNA strand breakage and in the activation of the ATM- dependent DNA damage signaling pathway [6,34–36] However, normal activation of the kinase activity of ATM and phosphorylation of the ATM ... Role of Nbs1 in ATM- dependent DNA damage signaling J.-H Lee and D.-S Lim DNA breaks Chromatin Fig Model for the dual role of Nbs1 in the ataxia telangiectasia mutated (ATM) dependent DNA damage signaling ... in the DNA damage response In addition, the extreme COOH-terminal region (amino acids 734–754) of Nbs1 mediates the interaction of Nbs1 with ATM and the recruitment of ATM to sites of DNA damage, ...

Ngày tải lên: 16/03/2014, 13:20

... Cisplatin-induced DNA damage response At sites of DNA damage, the Ser129-phosphorylated H2AX derivative, cH2AX, forms foci for the recruitment of factors involved in repair of DNA damage and maintenance of ... Cisplatin-induced DNA damage response Cisplatin DNA damage DNA damage H2AX- P ( H2AX) Recovery H2AX H2AX- P ( H2AX) Psy4 Pph3 MMS Recovery Pph3 Psy2 ATM/ ATR kinases recruited Psy2 ATM/ ATR kinases ... presence of forks and replication bubbles that prevent entry of the chromosomes into the gel [21,22] Treatment with MMS also sometimes resulted in the appearance of a ‘smear’ of low molecular mass DNA...

Ngày tải lên: 30/03/2014, 04:20

... microfuge tube Precipitate the DNA from this supernatant as in step Resuspend the precipitated DNA in 50 µL of TE buffer in a microfuge tube (see Note 19) 3.3.2 Labeling of 3'-OH Ends of DNA ... necrosis (10) 1.2 DNA Damage Detection In Situ: Potentials and Pitfalls The development of techniques for in situ end-labeling (ISEL) of fragmented DNA has allowed the recognition of DNA damage in single ... iodide signal (DNA content) on the X axis and TUNEL labeling (DNA breaks) on the Y axis 3.3 Determining the Nature of the Ends of the Fragments 3.3.1 Processing of Cells and Extraction of DNA Each...

Ngày tải lên: 11/04/2014, 09:47

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Ngày tải lên: 07/08/2014, 18:21

... http://www.ro-journal.com/content/6/1/7 DNA alkylating agents are used widely for treatment of a variety of pediatric and adult cancers because the cytotoxic effects of these agents can directly modify DNA and cause DNA lesions ... graphically markers for evaluating the level of DNA damage [24] To investigate if BO-1051 can affect the extent of DNA damage by g-rays, the formation of g-H2AX foci in cell nuclei was determined ... prolonged DNA damage response in glioma cells DNA damage is the most important biological effects caused by ionizing radiation It has been reported that the nuclear foci of g-H2AX is one of the...

Ngày tải lên: 09/08/2014, 09:20

... [24] Both LMB treatment of wt Tax and mutation of the NES (TaxM33) prevented the nuclear export of Tax following DNA damage [11] In addition, LMB treatment of wt Tax and mutation of the NES (TaxM33) ... resulted in cytoplasmic accumulation of the Tax protein In this study we examined the effects of DNA damage on the ubiquitination status of Tax and determined how DNA damage- induced changes in the post-translational ... results demonstrate that mono-ubiquitination of Tax occurs following DNA damage and that this modification may regulate the relocalization of Tax following DNA damage Lysine residues 280 and 284 regulate...

Ngày tải lên: 13/08/2014, 06:20

... 8-oxo-G DNA glycosylase hOGGl and the E coli DNA glycosylase AlkA kink error-free DNA in the same way they kink DNA during the excision of a damaged base [15] It is thus likely that some DNA glycosylases ... the stretch of DNA ahead of it is thus error-free Presumably the electron received destabilizes the binding of MutY to this error-free stretch of DNA by changing the oxidation level of the [4Fe-4S]3+ ... between damage to a base pair and detection of the error by MutY? The faulty base pair is either located by a MutY enzyme that happened to be bound to DNA downstream of the error at the time of damage...

Ngày tải lên: 13/08/2014, 22:22

... context, the type of DNA lesion, or the extent of damage, as p53-independent induction of this pathway was observed in other studies [19,20] interactions induction in response to DNA damage was abrogated ... use of RNA interference (RNAi) for targeted silencing of specific genes [5-7] The combination of global gene-expression profiling and RNAimediated silencing of key regulatory genes appears to offer ... transcriptional responses mediated by both ATM/ NFκB and ATM/ p53 We sought to demonstrate that this dissection of the ATM- mediated transcriptional network induced by DNA damage is precise and cannot reasonably...

Ngày tải lên: 14/08/2014, 14:21

... these DNA structures generates single-stranded DNA, the salient intermediate in the DNA- damage checkpoint response In fact, double-strand DNA breaks can also lead to stretches of single-stranded DNA ... Article 233 Cagney et al 233.3 of gene products are involved in DNA replication during the normal cell cycle, and the state of these proteins at the particular time of DNA damage may influence their ... machinery of DNA repair (Rnr1, Rnr2, Rnr4, Rfa1, Mag1, Crt1, Din7, Dun1) with proteins of the cell cycle, the stress response, and lipid and nucleotide metabolism reports In a recent study of DNA damage...

Ngày tải lên: 14/08/2014, 17:22

... the impact of influenza infection and inflammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA damage, we show that the level of DNA damage increases ... with DNA repair or replication fork breakdown [9, 10] In the presence of DNA damage, cells respond by eliciting DNA damage responses (DDR), which include activation of cell cycle arrest, DNA repair, ... with extensive DNA damage in many cell types during the course of disease Given that programmed cell death can be a consequence of unrepairable DNA damage, we evaluated the kinetics of apoptosis...

Ngày tải lên: 09/09/2015, 08:16

... role at G2 DNA damage checkpoint arrest, we also examined the role of Chk1, a canonical member of the ATM/ ATR pathway, in DNA damage- induced G2 checkpoint control While examining the role of p38 ... Effect of siRNA KD of p38, MK2 and Chk1 transcript on establishment of Adriamcyin induced G2 DNA damage checkpoint Figure 3.12: Effect of Adriamycin damage on HelaMK2-/- cells Figure 3.13: Effect of ... p38α and isoforms of p38, as they are the best characterized and understood isoforms of p38 This study does not discount isoform specific effects of p38 in response to stress and DNA damage, however...

Ngày tải lên: 11/09/2015, 09:02

... mechanism, the DNA damage checkpoint, inhibits cell cycle progression in response to DNA damage and causes cells to arrest in G2/M until the offending lesions are repaired A failure to repair DNA damage ... discussion of DNA damage checkpoint control and events associated with it, it is useful to begin with a brief description of the general regulatory landscape of the cell cycle in which the DNA damage ... pair with the initiating ssDNA, resulting in the extension of the initial DNA joint This process is termed "DNA strand exchange" or "DNA branch migration" The extent of DNA branch migration is determined...

Ngày tải lên: 14/09/2015, 08:23

... disease DNA damage response (DDR) pathway is a complex signaling network that has evolved to sense for DNA damages, activate cell signaling and repair DNA lesions DNA damage are surveyed via DNA damage ... INFLUENZA INFECTION INDUCES DNA DAMAGE AND ROBUST DNA DAMAGE RESPONSES IN VIVO 66 3.1 Introduction 66 3.1.1 Investigation of DNA damage as a potential mechanism of influenza pathogenesis ... following DNA damage DNA repair mechanisms are critical to neutralize genotoxic DNA damage, and they form an important component of DDR pathway Due to a wide diversity in DNA lesions, several DNA repair...

Ngày tải lên: 30/09/2015, 06:20

... 22-23 of 2003 The amount of precipitation was 30 mm in total Figure shows the precipitation record of the studied event (Fig 2a) and the record of the river flow rate (solid line, Fig 2b) of the ... measurement, considering of their respective analytical errors A concept of two-component mixing was employed, in which it was assumed that runoff is composed of a mix of two components: one originating ... and by setting C1 and C2 as mentioned above, the fraction of new water evaluated for the main part of the runoff (0000 of Oct 22 to 1600 of Oct 23, 40 h in total) was found to be as shown in Fig...

Ngày tải lên: 05/09/2013, 09:08

... coming out of the nozzle In this case study of rime ice generation was increased linearly with the spraying of water content Thickness of the ice averaged from values was 2.8cm Plots of dielectric ... Proof of concept Renewable Energy, 2012 50: p 196-205 [6] Kuroiwa, D., The Dielectric Property of Snow Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan [7] Basic of Measuring ... gun nozzle Capacitive ice detector of two rectangular aluminum bars used for this study was placed in front of exit section of ice generator at a distance of m, so that the flow gets stabilized...

Ngày tải lên: 05/09/2013, 15:28

... sites of DNA damage, and its role might be important in the presence of physiological levels of DNA damage or at the sites of a specific subset of DNA lesions In addition to the phosphorylation of ... recruitment of checkpoint sensors to specific types of DNA damage Both DNA damage and the activation of checkpoint can lead to changes in the chromatin structure at the sites of DNA damage (see ... possible that the interactions of ATR-ATRIP with additional proteins on DNA also contribute to the localization of ATR-ATRIP to specific types of DNA damage 2.2 DNA Damage Recognition by the RFC-...

Ngày tải lên: 25/10/2013, 21:20

... cm, despite the present of Dau Tieng reservoir, the downstream of the Dong Nai river will still be flooded up to 2950.8 km2 of which 58% of areas is inundated under 1m, 40% of areas is inundated ... rises, the extent of area inundated from 1m to 2m depth is also increased In general, the downstream area of the Dong Nai river basin has relatively large inundated area due to effects of climate change ... discharge of 4500 m3/s at Tri An station and of 600 m3/s at Dau Tieng station, in October 2000 was used to calibrate the model Another flood in August 2008 was used to verify the model Results of calibration...

Ngày tải lên: 13/02/2014, 12:20