dna and dna repair targeted therapy of non clear cell carcinoma

... defined in the activation of EGFR-dependent intracellular signaling First, the binding of a receptor-specific ligand occurs in the extracellular portion of the EGFR or of one of the EGFR-related receptors ... induction of apoptosis in cell lines with mutant EGFR [22] The combination of chemotherapy and TKI in some cases may cause a G1 arrest of growth that blocks the subsequent effects of chemotherapy ... Watson D, Green M, Cancer and Leukemia Group B: Lack of trastuzumab activity in non- small -cell lung carcinoma with overexpression of erbB2: 39810: a phase II trial of Cancer and Leukemia Group B Cancer...

Ngày tải lên: 10/08/2014, 22:20

... In Kathmandu, capital of Nepal, tens of thousands celebrate the ending of the period of royal rule and martial law, 27 April 2006 3.1 Gandhi and Jawaharlal Nehru, July 1946 41 48 3.2 Gandhi on ... member of the Department of Political Studies at the University of Witwatersrand between 1978 and 2005 He is the author of Black Politics in South Africa since 1945 (Ravan Press, 1983) and Mandela: ... exposition of the variety of forms of non violent action is Gene Sharp, The Politics of Nonviolent Action (Boston: Porter Sargent, 1973) Based on his 1968 Oxford D.Phil thesis, it began as a study of non...

Ngày tải lên: 11/06/2014, 10:33

... Effects of VLPs IL-10 shRNA on morphology and viability of RAW 264.7 cells (A) Two preparations of IL-10 shRNA (IL-10i-1 and IL-10i2) were packaged into JCV VLPs by osmotic shock The presence of IL-10 ... contributions MIC and YFH contributed equally to this work MIC and YFH interpreted the data and drafted the manuscript MIC, MW, DC and JTC designed and performed the RNAi and JC virus experiments MZ and GJT ... μg of JCV VLPs with IL-10 shRNA, or irrelevant shRNA, respectively After 6, 12 and 24 h of treatments, serum samples were collected and tested for IL-10 and TNF-α levels by real-time PCR and...

Ngày tải lên: 10/08/2014, 05:21

... the treatment of patients with NSCLC Materials and methods Page of amount of tumor cells in order to ensure that they contained more than 70% of tumor components for DNA extraction and mutation ... PCR Amplification and sequencing The two codons of KRAS (12 and 13) and two exons of EGFR (19 and 21) were amplified by PCR using the following forward and reverse primers: exon of KRAS: 5’AAAGGTACTGGTGGAGTATTTGATAGTG-3’, ... extract genomic DNA Quality and concentration of the DNA samples were examined by Nano Drop (Thermo™) Genomic DNA was then diluted to a working concentration of 5-10 ng/ul Patients and samples Patients...

Ngày tải lên: 10/08/2014, 10:21

... hour of each gene) Relative mineralization of FGF-2 and BMP-2 treated cells As seen in Figure and Table 3, BMP-2 treatment enhances mineralization of the cells as shown by uptake and presence of ... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly ... mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related...

Ngày tải lên: 20/06/2014, 04:20

... hour of each gene) Relative mineralization of FGF-2 and BMP-2 treated cells As seen in Figure and Table 3, BMP-2 treatment enhances mineralization of the cells as shown by uptake and presence of ... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly ... mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related...

Ngày tải lên: 20/06/2014, 07:20

... fragmentation of nuclear DNA by BNCT Induction of apoptotic cells with the fragmentation of nuclear DNA by BNCT SAS/neo and SAS/mp53 cells were treated with BNCT, incubated for 48 h at 37°C, and stained ... SAS/mp53 cells At a dose of Gy, the Figure BNCT Suppression of the colony formation of oral SCC cells by Suppression of the colony formation of oral SCC cells by BNCT SAS/neo and SAS/mp53 cells ... fractions of SAS/neo and SAS/mp53 cells were and 36%, respectively (Figure 1) Suppression of the proliferation of oral SCC cells by BNCT To determine the effect of BNCT on the proliferation of cells,...

Ngày tải lên: 09/08/2014, 10:20

... radiotherapy and radiochemotherapy will be required for further analysis of this important aspect of adaptive radiotherapy KB developed in-house software for voxel-based analysis of CT image and ... 124 cm3 (19 cm3 - 325 cm3) and 100 cm3 (10 cm3 - 270 cm3) mid-way and at the end of radiochemotherapy Comparison of CTplan and CTFU was performed for quantification of anatomical changes during ... reported residual errors of landmark registration ranging between mm and mm on average depending on the DR algorithm and type of landmark structures [17,18,20-22] Manual landmark registration was...

Ngày tải lên: 09/08/2014, 10:20

... histology: adenocarcinoma (AC), squamous cell carcinoma (SqCC), and large cell carcinoma (LCC) To study the relationship between protein expression and survival in non- small cell lung cancer, ... (4) Non- Specified 321 (24) (6) n/a Race Histology Adenocarcinoma 603 (46) 58 (55) 51 (66) Large Cell Carcinoma 75 (6) 18 (17) 15 (19) Squamous Cell Carcinoma 338 (26) 15 (14) 11 (14) NSCLC Non- Specified ... RTK and EMT could have lower values due to data reduction In addition, there was a trend towards higher protein expression in adenocarcinoma and large cell carcinoma than in squamous cell carcinoma; ...

Ngày tải lên: 10/08/2014, 09:22

... percentage of apoptotic cells was calculated as the number of apoptotic cells per number of total cells × 100% The mock or stably transfected A549 cells were seeded into 96-well plates (6.0 × 103 cells/well) ... upregulation of miR-451 expression in A549/miR-451 cells (Figure 2B) Page of 11 Upregulation of miR-451 inhibits growth and enhances apoptosis of NSCLC cell line (A549) To analyze the effect of miR-451 ... with those of A549/miR-NC and mock A549 cells (Figure 5A and 5B) The cells were treated μg/ml DDP for 12 h and the number of colonies was determined As shown in Figure 5C, the number of colonies...

Ngày tải lên: 10/08/2014, 10:21

... power microscope, and then randomly selected fields under high power (200×) light microscope For assessment of staining positivity, the number of positive cells out of 200 tumor cells in each field ... received cycles of adjuvant platinum based two drug chemotherapy Among them, 28 patients received post-operative combined chemotherapy and thoracic radiotherapy and 22 patients had chemotherapy alone ... found in squamous cell (70%) followed by adenocarcinoma (50%) [29], and large cell carcinomas [28], in our study, EGFR positivity rates were similar between squamous carcinoma (40%) and adenocarcinorma...

Ngày tải lên: 10/08/2014, 10:21

... Dianying, Shen Yali, Xu Feng, Wang Jin: EGFR and COX-2 protein expression in non- small cell lung cancer and the correlation with clinical features Journal of Experimental & Clinical Cancer Research ... article as: Li et al.: Correction: EGFR and COX-2 protein expression in non- small cell lung cancer and the correlation with clinical features Journal of Experimental & Clinical Cancer Research ... 50.0% Adencarcinoma 26 100% 66.70% Mixed type 100% Tumor length ≤3 cm 14 87.50% > cm 31 91.20% Tumor length ≤3 cm 0.535 43.80% > cm 18 16 47.10% Level of Differentiation 0.474 0.518 Level of Differentiation...

Ngày tải lên: 10/08/2014, 10:21

... angiogenesis and neoplastic proliferation [14], the association of NF-B with lymphangiogenesis in ESCC is less clear Members of the Notch family of cell surface receptors and their ligands also ... positive association of NF-B and VEGF-C expression with lymph node metastasis, independent of T stage, sex, age, and differentiation of tumor cells 0.002 Association of NF-B and Notch1 with tumor-induced ... dehydrated, and mounted Negative controls were prepared by substituting PBS for primary antibody Assessment of immunohistochemical staining Nuclear staining of NF-B and cytoplasmic staining of Notch1 and...

Ngày tải lên: 10/08/2014, 10:21

... http://www.head-face-med.com/content/4/1/7 midhand and in the bones of the foot [5] As to our knowledge, no case has been reported up to date on the manifestation of distant metastases of a SCC in the soft tissue of the extremities ... and adjunctive chemotherapy in the management of squamous cell carcinoma of the head and neck region A meta-analysis of prospective and randomized trials J Clin Oncol 1996, 14:838-847 Jeremic B, ... mediastinum 2.9%, lung and bone 3.3% and others 15.4% Primary tumor in the nasopharynx: bone 54%, lung 23.8%; primary tumors of the fossa tonsillaris and of the basis of the tongue: metastases...

Ngày tải lên: 12/08/2014, 01:22

... Vaccines and Therapy 2005, 3:5 Background Lung cancer is a leading cause of cancer death in Australia and the world [1,2] There are two types of lung cancers, non small cell (NSCLC) and small cell ... The effects of dsRNA-EGFR on A549 (A) and SPC-A1 (B) cell count and colony formation (C) The effects of dsRNA-EGFR on A549 (A) and SPC-A1 (B) cell count and colony formation (C) Cells were seeded ... and SPC-A1 cells At a multiplicity of infection (MOI) of 10 and 72 hours after transduction, RNAi-mediated gene silencing of EGFR was apparent About 75% of A549 and 80% of SPC-A1 cells became...

Ngày tải lên: 14/08/2014, 19:22

... tumor-infiltrating lymphocytes for adoptive therapy of renal cell carcinoma (RCC) and metastatic melanoma: sequential proliferation of cytotoxic natural killer and noncytotoxic T cells in RCC J Immunother 1991, ... Wernet P, Ackermann R: Novel mutations of the von Hippel-Lindau tumor-suppressor gene and rare DNA hypermethylation in renal cell carcinoma cell lines of the clear cell type Int J Cancer 2000, 87:650-653 ... R, Belldegrun A: Induction of G250 -targeted and T -cell- mediated antitumor activity against renal cell carcinoma using a chimeric fusion protein consisting of G250 and granulocyte/monocyte-colony...

Ngày tải lên: 18/06/2014, 16:20

... cinoma of the kidney Clear- cell carcinoma of the breast, resembling clear- cell carClear -cell carcinoma of the breast, resembling clear- cell carcinoma of the kidney The lobular arrangement and the ... the benign clear cell (sugar) tumor, which contains abundant glycogen [10,11] and the clear carcinoma, which contains abundant mucin [12] Clear cell carcinoma of the larynx, a variant of mucoepidermoid ... Discussion Glycogen-rich clear cell carcinoma of the breast is a rare tumor It is, however, the most frequent cause of clear cell morphology in breast malignancies [5] It is composed of cells containing...

Ngày tải lên: 09/08/2014, 07:21

... Page of Netherlands) The brachytherapy dose for each insertion was or Gy at point A, and the total dose of brachytherapy was 24-35 Gy (median 24 Gy) All patients also were given concurrent chemotherapy, ... conception and design SHM and JYK helped with the database and data analysis SSS and SYP provided additional guidance and support for this research All authors read and approved the final version of ... Tumor-associated antigen, TA-4, in the monitoring of the effects of therapy for squamous cell carcinoma of the uterine cervix Serial determinations and tissue localization Cancer 1985, 56:302-308...

Ngày tải lên: 09/08/2014, 09:20

... facilitation of complete tumour clearance (Figure 4) Always the cavotomy is repaired with the use of a pericardial patch (Figure 5), in order to avoid narrowing of the cava An algorithm of the plan of ... showed renal cell carcinoma of clear type in patients and papillary type in patient Lymph node metastasis was detected in patients Two of the discharged patients were lost to follow up Of the remaining ... involvement ↓ ↓ LEVEL III (12-15% of the cases) LEVEL IV (25% of the cases) CPB, Pringle manoeuvre and if necessary Always use of CPB and brief period of cross clamp of subdiaphragmatic aorta TCA...

Ngày tải lên: 10/08/2014, 09:22

... activity of DNA- PKcs could be specifically inhibited by As-ODNs and resulted in marked inhibition of DNA- Dsb rejoining and radiosensitization of human non- small cell lung cancer (NSCLC) cell line ... the cell cycle at G /S, S, and G /M to prevent the processing of damaged DNA; (2) it activates DNA- repair pathways; and (3) it induces apoptosis if the DNA damage is so detrimental that normal cell ... percentage of positive staining cells, namely AI = number of apoptotic cells × 100/total number of nucleated cells Statistics Results were expressed as mean ± standard deviation (SD) and were analyzed...

Ngày tải lên: 10/08/2014, 10:21