... andgender. Instead of asking how the gender divisionoflabor withinfamilies varies across locations within a class structure, the focus of analysis would be on how the gender divisionoflabor varies ... gender is the problem of bargaining power between men and women within households. Parti-cularly in an era in which gender roles are being challenged, the division oflaborin the household should ... gender divisionoflabor within families. Acomparison of inequalities in housework in the two countries, therefore,may give some insight into the extent to which this egalitarianism in thepublic...
... confirmed by sequencing DNA samples of 51 healthy individuals. Interestingly, this SNP had not yet been registered in any SNP databases despite of its high frequency of 20% in samples of patients ... of samples containing the C/T SNP downstream of exon 10 led to the identification of cleavage products of approximately 101 bp and 444 bp in size in samples either containing the heterozygous ... (SNP) in the intron sequence flanking the 3’ end of exon 10 in 7 out of 35 (20%) patient samples. The SNP consisted of a C > T exchange (Figure 1 C-E) and was positioned at nt 67 of the intron...
... was obtained with scFv4A-d1-EGFP and 7A-d1-EGFP expressed in parallel.Interestingly, in the case of 4A (but not 7A), we founda minor percentage ofcells with a faint cytoplasmicstaining (arrow). ... FRAP:measuring protein mobility and activity in living cells. Nature Cell Biol 3, 145–147.34 Bachmair A, Finley D & Varshavsky AK (1986) In vivohalf-life of a protein is a function of its amino-terminalresidue. ... fused to d1-EGFP are maintained in the cells by binding to antigen.Generation of soluble binding-defectiveand cysteine-deleted mutantsTo demonstrate that the observed intracellular colocal-ization...
... POUhomeodomain family proteins bind to this consensus motifand regulate gene transcription in diverse cells throughinteraction with a variety of partner DNA binding proteins,including Sp1 family members ... thepivotal role of fgl2 in a murine model for murine hepatitisvirus strain 3 (MHV-3) induced fulminant hepatic failure[21,22]. Administration of neutralizing antibodies againstfgl2 conferred ... development of a normal fetus and in regulatingimmune responses within the intestine. Induction of fgl2 in macrophages in response to viruses results in theproduction of a potent coagulant activity...
... withuntreated cells. As expected, cells exposed to KN93also developed more neurites than control cells, point-ing to a key role of this kinase in the control of neuritogenesis in N2a cells (Fig. 5E).Involvement ... currents in this cell line. Pharmacological inhibition of the P2X7 receptor and its knockdown by small hairpin RNA interferenceresulted in increased neuritogenesis incells cultured in low serum-contain-ing ... adhesion kinase is upstream of phosphati-dylinositol 3-kinase ⁄ Akt in regulating fibroblast survival in response to contraction of type I collagen matricesvia a beta 1 integrin viability signaling...
... many proteins involved in apoptosis, including the expression profiles of proteins involved in the mito-chondrial (bcl-2, bax, and bcl-x, etc.) and stress reac-tion-related pathways of apoptosis ... transfected cells and control cells. Interestingly, the phosphorylation of JNK/SAPK was inhibited by the Int. J. Med. Sci. 2007, 4 40Figure 2 PowerBlot patterns of JAR cells. Forty elements of ... overexpressed in cytotrophoblasts, and a decrease in the expression of Bcl-2 and increase of apoptotic cells are seen in preeclampsia. Thus antiapoptotic proteins can play an important role in preventing...
... signaling. This finding corroborates the interaction observed between various intracellular signaling pathways and apoptosis induced by 1mM concentration of ethanol in HepG2 cells [19]. Int. ... . Interestingly, HMGN3 has been found to be associated with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma ... (including Kupffer cells) [41, 42], and ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44]. These...
... its initial effect on protein kinases phosphorylation. The most strikingly and novel finding obtained hereby was the strong inhibition of the GPCR signaling pathway induced mainly by increased ... of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. 24. Pelech S, Sutter C, Zhang H. Kinetworks protein kinase multiblot analysis. ... Kinne RKH. Effects of doxorubicin, mitomycin C, and ethanol on Hep-G2 cellsin vitro. J Cancer Res Clin Oncol 1999;125:1-8. 26. Higashi K, Hoshino M, Nomura T, et al. Interaction of protein...
... expression of claudin-1 in the cytoplasm, with some nuclear expression of claudin-1. B) Treatment of these cells with PKA inhibitor results in nuclear translocation of claudin-1 in as little as 15 minutes, ... is involved in claudin-1 expression and localization. Indeed, a study shows that increasing β-catenin in- creases the expression of claudin-1 in colorectal cancer cells, and that claudin-1 ... lo-calization of claudin-1. Nuclear claudin-1 does not increase melanoma cell motility. We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4)....
... long-chain saturatedfatty acids (SFAs) led to increased expression of proin-flammatory cytokines, inhibition of insulin signaling,induction of endoplasmic reticulum (ER) stress, andpromotion of ... carcinoma cells were grown in DMEM containing 10% heat-inactivated fetal bovineserum, 2 mm glutamine, 100 UặmL)1penicillin, and100 ngặmL)1streptomycin, at 37 C in air with 5% CO2. Cells ... in two phases according to the intensityand the duration of the stress. An initial adaptiveresponse culminates in the temporary inhibition of protein synthesis, providing cells with the opportunityto...
... pHb-1 cells resulted in a large reduction ofin vivo and in vitroinvasive capacity, as well as in vitro motility [12,13].The reduction in S100A4 also resulted in a decrease in the expression of ... two cell lines was determined asdescribed in Materials and methods, and 81 lg of pro-tein was added to each well. The amount of S100A4 in the II-11b cells was 1.2% of that in the pHb-1 cells after ... of S100A4 in pHb-1 and II-11b cells. (A) Deter-mination of S100A4 by western blotting of cell lysates from pHb-1and II-11b cells attached to plastic, using a total protein concentra-tion of...