... digesting the fragment with HindIII and Bgl II andthe plasmid with HindIII and Bam HI. The fragment was ligated into the plasmid andthe integrity of the insertion was validated by digesting the plasmidconstruct ... atpositions 597, 641, 667, and 691.To construct the GST fusions ofthe tandem SH2 domains of each SHP-1 and SHP-2, the sequences corresponding toamino acids 2–232 of each SHP-1 and SHP-2 wereamplified ... with Eco RI and Bbr PI, then gel purified(< 820 bp). The insert was then ligated into the pSPORTvector andthe resulting full-length siglec-10 clone wasdesignated 995-2 andthe sequence...
... human and ratCB1receptor. The CB2antibody was raised against asequence between the N-terminal andthe first trans-membrane domain ofthe protein ofthe human and ratCB2receptor. The specificity ... marrow stem cells, are the most potent antigen-presenting cells ofthe immune system. They play a central role in the initiation of primaryimmune response and in the enhancement of secondaryimmune ... as far as the amounts of the endogenous ligands are concerned, but differently interms ofthe levels ofthe two cannabinoid receptor subtypes and FAAH. Indeed, inflammatory, allergenic and septicstimuli...
... in the compliance ofthe chest wall, the lung, andthe respiratory system between an ‘‘ideal’’ 20-year-old (A) and a 60-year-old subject (B). Note increase in RV and FRC and decrease in slope of pressure-volume ... chest-wallcompliance, and increase in functional residualcapacity (FRC) resulting from decreased elastic recoil of the lung (Fig. 2) [9]. The kyphotic curvature of the spine andthe anteroposterior diameter ofthe ... ity-basedaging ofthe respiratory system 483 by the same group, based on a larger communitysample of 4581 persons ages 65 and older, reports anupper limit of normal of 29% for PEF lability. A cut-off...
... III(4) ofthe Treaty of Amsterdam. 1345.2. The General Council shall comprise the President and Vice-President ofthe ECB andthe Governors ofthe nationalcentral banks. The other members ofthe ... 17 ofthe Act concerning the conditions of Accession ofthe Czech Republic, the Republic of Estonia, the Republic of Cyprus, the Republic of Latvia, the Republic of Lithuania, the Republic of ... annual report on the activities ofthe ESCB and on the monetary policy of both the previous andthe current year to the European Parliament, the Council and the Commission, and also to the European...
... consists of two com-ponents which of ten have opposing func-tions: the sympathetic nervous system and the parasympathetic nervous system . The interac-tion of these two systems keeps the interiororganization ... include the peripheral nerves of the autonomic nervoussystem in the PNS. The CNS develops from the neural plate (D4) of the ectoderm which then transforms into the neural groove (D5) and further ... interiororganization ofthe organism constant.In vertebrates, the somatic nervous system developed in addition to the autonomic nervous system; it consists of the central nervous system (CNS; brain and spinal...
... scientific theory and there are many examples in the history of science that tell us so. The geocentric theory ofthe solar system, the phlogistontheory of burning andthe concept of chemical ... between planets A and B 2469 The Moon 2519.1 The origin ofthe Earth–Moon system 2519.1.1 The fission hypothesis 2519.1.2 Co-accretion ofthe Earth andthe Moon 2549.1.3 Capture ofthe Moon from ... observed on the satellite. The basis ofthe prediction was the 2:1 ratio ofthe periods of Io and Europa. Because of this the nearest approach, and hence the maximum perturba-tion, of Io by Europa...
... (Invitrogen), and the isolation of RNA followed the manufacture’sprotocol. The synthesis of first-strand cDNA was per-formed using the Superscript preamplification system (Invitrogen). Either 5 lg of t ... of the product of P450scc-mediated side-chaincleavage of 7-DHC. (A) Spectrum of the enzymatic side-chain cleavage of 7-DHC;(B) spectrum of 7-DHP synthetic standard.Ó FEBS 2004 P450scc in the ... illustrates mass spectra of isolated7-DHP-MO-TMS, andthe synthesized standard. The molecular ion is at m/z 415, and distinctive ions are formedby loss ofthe silylated hydroxy, methyl and oxime groupsat...