... digesting the fragment with HindIII and Bgl II and the plasmid with HindIII and Bam HI. The fragment was ligated into the plasmid and the integrity of the insertion was validated by digesting the plasmid construct ... at positions 597, 641, 667, and 691. To construct the GST fusions of the tandem SH2 domains of each SHP-1 and SHP-2, the sequences corresponding to amino acids 2–232 of each SHP-1 and SHP-2 were amplified ... with Eco RI and Bbr PI, then gel purified (< 820 bp). The insert was then ligated into the pSPORT vector and the resulting full-length siglec-10 clone was designated 995-2 and the sequence...
Ngày tải lên: 24/03/2014, 04:21
... transfer of the ligand and receptor to a lysosome (L) where they are degraded; 3 the receptor and ligand are transferred to a compartment of uncoupling of receptor and ligand (CURL). The receptor and ... (fig. 1.16). These make up 6–8% of the total endothelial cell surface, and there are more in the centrilobular zone of the sinusoid than the peri-portal area. Extra-cellular matrix affects their function. Fenestrae ... contains the microsomes. It is the site of bilirubin conjugation and the detoxification of many drugs and other foreign compounds (P450 systems). Steroids are synthesized, including cholesterol and the primary...
Ngày tải lên: 10/08/2014, 15:20