diseases of the nervous system and their symptoms

Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx

... digesting the fragment with HindIII and Bgl II and the plasmid with HindIII and Bam HI. The fragment was ligated into the plasmid and the integrity of the insertion was validated by digesting the plasmid construct ... at positions 597, 641, 667, and 691. To construct the GST fusions of the tandem SH2 domains of each SHP-1 and SHP-2, the sequences corresponding to amino acids 2–232 of each SHP-1 and SHP-2 were ampliﬁed ... with Eco RI and Bbr PI, then gel puriﬁed (< 820 bp). The insert was then ligated into the pSPORT vector and the resulting full-length siglec-10 clone was designated 995-2 and the sequence...