discussion on substrate and high oxygen pressure heterostructures

Host viral interactions host factors in coronavirus replication and coronaviral strategies of immune evasion

Ngày tải lên : 09/09/2015, 10:07

... overexpression Figure 4-10: Effect of TLR3 over-expression on IBV replication Figure 4-11: Comparison of RIG-I and MDA5 expression in IBV and dengue infected cells Figure 4-12: Effect of IBV infection on ... regulate coronavirus translation in a similar fashion 1.2.1.4 Mitochondrial aconitase Despite the lack of a consensus RNA-binding domain, mitochondrial aconitase (maconitase) was one of four ... 4.2.3 DISCUSSION ………………………………………………………………….166 CHAPTER FIVE: GENERAL DISCUSSION AND FUTURE DIRECTIONS 5.1 GENOME-WIDE SCREEN REVEALS CELLULAR CO-FACTORS IN CORONAVIRUS INFECTION 5.1.1 Main conclusions……………………………………………………………………….170...

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Báo cáo y học: " Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" docx

Ngày tải lên : 13/08/2014, 09:20

... Sattentau QJ: Interactions among HIV gp120, CD4, and CXCR4: dependence on CD4 expression level, gp120 viral origin, conservation of the gp120 COOHand NH2-termini and V1/V2 and V3 loops, and sensitivity ... action in inhibiting binding of gp120 to CCR5 and DCSIGN, and, hence, virus dissemination Methods Cloning, protein expression and purification i) Env-based proteins Fc-gp120 chimeras were constructed ... infection assays TZM-BL is a HeLa cell clone that expresses CD4, CXCR4 and CCR5 and contains Tat-responsive reporter genes for firefly luciferase and β-galactosidase under the control of a HIV long...

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Báo cáo y học: " Correction: Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" doc

Ngày tải lên : 13/08/2014, 09:20

... 60 standard post-CD4 40 20 0.001 0.01 0.1 10 mAb concentration (µg/ml) % residual infection C) 120 2G12 (high mannose) 100 standard post-CD4 80 60 40 20 0.001 0.01 0.1 10 mAb concentration (µg/ml) ... residual infection A) http://www.retrovirology.com/content/4/1/23 b12 (CD4bs) 120 100 standard post-CD4 80 60 40 20 0.001 0.01 0.1 10 mAb concentration (µg/ml) % residual infection B) X5 (CD4i) ... neutralization activity of mAbs A) b12, B) X5, and C) 2G12 was assessed in the standard (closed circles) and post-CD4 (open circles) neutralization formats Results are expressed as % of residual infection,...

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Tài liệu Báo cáo Y học: A pool of Y2 neuropeptide Y receptors activated by modiﬁers of membrane sulfhydryl or cholesterol balance pot

Ngày tải lên : 22/02/2014, 04:20

... surface-washed and assayed (see Materials and methods) Data represent triplicate competition assays employing 8–10 concentration points in the range of 10–30 000 pM nonlabeled Y2 agonist, and 50 pM ... internalization relative to controls, and also neutralized any decrease in receptor- linked Y2 ligand internalization by PAO (Fig 2) Effects of the alkylators NEM and MTSET Effects of alkylators on availability ... to saturate between 10 and 100 lM (Fig 3), and was followed by a signiﬁcant decrease at 300 lM, reﬂecting inactivation of a fraction of the Y2 sites at high concentrations of the alkylator [14]...

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DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf

Ngày tải lên : 08/03/2014, 19:20

... between replication and recombination is also demonstrated in common posttranslational modifications Posttranslational modifications, with their key role in controlling protein function, play an important ... deletions, inversions, or translocations when non-allelic or repeated sequences are present The Relationship Between Replication and Recombination 39 3.2 Chromatid cohesion and recombination mechanisms ... accumulation of ssDNA, which recruits additional RPA and causes activation of the checkpoint kinases ATR and Chk1 The formation of replication forks and the generation of ssDNA both require MCM function...

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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx

Ngày tải lên : 18/06/2014, 15:20

... expression of genes encoding cytokines and chemokines and anti HBsAg serum titers in vaccinated healthy donors Correlation Correlation between expression of genes encoding cytokines and chemokines and ... Phenotypic characterization of cells PBMC were phenotyped by staining with FITC- or PE-conjugated mouse monoclonal antibodies (mAb) to human CD8 and CD4 (Becton Dickinson, San Diego, CA) CD8+ ... evaluated and correlated with antigen driven gene expression data Comparative assays were performed with samples from two seropositive and one seronegative donor HBsAg was able to induce IL-2 and IFN-γ...

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Báo cáo sinh học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" pptx

Ngày tải lên : 18/06/2014, 18:20

... outcomes Authors' contributions YWL carried out the experiments on apoptosis and contributed to the first draft of manuscript TLT contributed in cloning of Bcl-2 family of genes, and helped in Western ... in a higher level of liver cell proliferation in a regeneration effort Such an increase in cell division may perturb the normal cell cycle control, resulting in an accumulation of mutations in ... Result was generated and compared with other BH3 domains Results and discussion FACS results A cell-based system for HBV genome A, B, and C replication was generated by cloning the linearized...

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Báo cáo hóa học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" docx

Ngày tải lên : 20/06/2014, 01:20

... outcomes Authors' contributions YWL carried out the experiments on apoptosis and contributed to the first draft of manuscript TLT contributed in cloning of Bcl-2 family of genes, and helped in Western ... in a higher level of liver cell proliferation in a regeneration effort Such an increase in cell division may perturb the normal cell cycle control, resulting in an accumulation of mutations in ... Result was generated and compared with other BH3 domains Results and discussion FACS results A cell-based system for HBV genome A, B, and C replication was generated by cloning the linearized...

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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

Ngày tải lên : 16/03/2014, 02:20

... pretreatment on the effect of insulin on the expression of PEPCK and another gluconeogenic enzyme, G6Pase Compared with the control, insulin incubation caused a signiﬁcant inhibition of PEPCK and G6Pase ... prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis and glucose output in HepG2 cells Although TNFa-mediated inhibition ... Journal compilation ª 2009 FEBS A K Pandey et al Effect of TNFa on insulin action on PEPCK expression preincubated with TNFa followed by insulin treatment On termination of incubation, cells were...

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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

Ngày tải lên : 16/03/2014, 12:20

... (N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide) (200 nm final concentration); chelerytrine (200 lm final concentration), heparin (500 lgÆmL)1 final concentration), and PKI (10 nm final concentration) Except for heparin and PKI ... strand These interactions determine the formation of an open complex at the CT element composed of unpaired strands, CNBP, hnRNP K, and additional factors [7] Modulation of the concentrations and ... concentration of nm, and accurate molar amounts of fusion proteins were added as indicated Final reaction volume was 20 lL Binding reactions were incubated for 30 at 37 °C and then loaded onto...

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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

Ngày tải lên : 16/03/2014, 22:20

... sensi3566 tivity of detection by the technique of tissue section in situ hybridization Discussion The differentiation and development of primary motor neurons in vertebrates is controlled by RA signaling ... of both exon (114 bp), intron (3262 bp) and exon (298 bp), and part of the sequence for intron The cDNA and genomic DNA sequences showed differences between three nucleotides in exon 2, resulting ... bourg, Association pour la Recherche sur le Cancer, Ligue Nationale Contre le Cancer, National Institute of Health (to CT and BT), and Izaak Walton Killam Memorial Scholarships (to R-ZL and MKS) We...

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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt

Ngày tải lên : 17/03/2014, 17:20

... thrombin apparently depends on continuous generation of InsP3 and is down-regulated by elevation of the cAMP concentration (see also below) Note that similar, irregular Ca2+ responses were also obtained ... spiking and to a lesser extent the thimerosal/U73122-induced spiking appears to be sensitive to cAMP modulation Regulation of InsP3 receptor function and store depletion by Ca2+, cAMP and sulfhydryl ... at 10 after saponin Increases in [Ca2+] were measured in response to the agonist (thrombin, thimerosal or U73122) and InsP3 The Ca2+ release by 50 nM InsP3 under control conditions (no pretreatment)...

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Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt

Ngày tải lên : 30/03/2014, 08:20

... K+-free medium Second, we compared the dose-dependent action of ouabain on intracellular monovalent cation content and c-Fos expression This experiment revealed that c-Fos accumulation in ouabain-treated ... ouabain and monensin on c-Fos content in HeLa cells (B) Dose-dependent effect of ouabain on intracellular Na+, K+ and c-Fos content Cells were incubated for h in DMEM with or without ouabain and monensin ... the accumulation of charge-balancing anions, such as Cl– and HCO3– Elevation of monensin concentration up to 10 lgÆmL)1 attenuated cell survival, as indicated by the accumulation of trypan blue-posit-...

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Báo cáo khoa học: Binding areas of urokinase-type plasminogen activator– plasminogen activator inhibitor-1 complex for endocytosis receptors of the low-density lipoprotein receptor family, determined by site-directed mutagenesis doc

Ngày tải lên : 30/03/2014, 10:20

... receptor-bound ligand, [R]T is total receptor concentration, [L] is the concentration of free ligand, and Kd is the equilibrium dissociation constant With the 20 pm concentrations of 125I-labeled ligands ... association was followed by a second, slower phase, and a corresponding rapid dissociation phase, amounting to 20% of total binding, particularly with relatively high ligand concentrations We therefore ... dissociation rate constant from the slow phase of dissociation (see Experimental procedures) and calculated the Kd values as the ratio between the association and dissociation rate constants (Table...

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báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx

Ngày tải lên : 19/06/2014, 22:20

... infrequently detected Discussion Mononuclear phagocytes are central components of brain lesions in MS and are believed to be effector cells causing demyelination and axonal injury in MS [38] ... aspects mimics MS, with the formation of focal areas of demyelination [31] and axonal injury and loss [32] Our results demonstrate a prominent accumulation of monocytes and macrophages expressing CCR1, ... distribution Immunoneutralization of CCL2 [21], and genomic deletions of CCR2 [23,25,26], or CCL2 [59] result in a decreased susceptibility to EAE and reduced mononuclear cell infiltration In a...

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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf

Ngày tải lên : 19/06/2014, 22:20

... 1% nonessential aminoacid, 1% sodium pyruvate and 1% antibiotic penicillin and streptomycin (all from Gibco, Scotland, UK) and were maintained under standard cell culture conditions at 37°C and ... environment in both physiologic and pathologic conditions Previous work in our lab has demonstrated that sVCAM-1 is constitutively expressed on b End5 cells and increased by TMEV infection [24] ... what is happening as consequence of increasing AEA tone under the conditions of our study due to the multiple cellular targets for AEA actions on the responses to TMEV infection Nevertheless, we...

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báo cáo hóa học: " Glrt-Based Array Receivers for The Detection of a Known Signal with Unknown Parameters Corrupted by " docx

Ngày tải lên : 21/06/2014, 00:20

... and C (nT) ∆ E[x (nT) = = v x v v x v xv(nT)T] respectively, where T and † correspond to the transposition and transposition conjugation operation respectively Under the assumptions of section ... formulation We consider the detection problem with two hypotheses H0 and H1, where H0 and H1 correspond to the presence of total noise only and signal plus total noise in the observation vector ... where ⊗ is the convolution operation The signal s(t) may = correspond to the synchronization preamble of a radio communications link For example, each burst of the military 4285 HF standard is composed...

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Báo cáo hóa học: " Research Article Optimal Design of Nonuniform Linear Arrays in Cellular Systems by Out-of-Cell Interference Minimization" ppt

Ngày tải lên : 22/06/2014, 19:20

... antenna gain and the carrier frequency, and α is the path loss exponent The contribution of shadowing in (12) will be considered in Section 5.3 as part of an additional log-normal random scaling ... power minimization approach, in this case, functional dependence of the performance criterion (7) on the antenna spacings Δ is highly nonlinear (see Section for further details) and complicated ... approximation In a richer scattering environment, the conditions on the an1 that justify the use of rank-1 approxgular spread φi imation can not be considered to hold Therefore, a rank-a approximation...

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The rheumatoid arthritis shared epitope increases cellular susceptibility to oxidative stress by antagonizing an adenosine-mediated anti-oxidative pathway ppt

Ngày tải lên : 09/08/2014, 10:20

... digitized camera mounted on a Nikon Eclipse E400 (Nikon USA, Melville, NY, USA) microscope, using Scion Image software (Scion Corporation, Frederick, MD, USA) Quantification of comet fluorescence ... its natural conformation As can be seen, such peptides inhibited forskolin-induced AC activation (Figure 1d) and generation of cAMP (Figure 1e), resulting in inhibition of PKA activation (Figure ... diametrically opposite effects of the SE on the cAMP- and the NO-mediated pathways on the one hand and the proposed role of oxidative stress in the pathogenesis of RA [10-15] on the other, we proceeded to...

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