discovery validation and mapping of gene associated snps
báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
Ngày tải lên :
12/08/2014, 03:20
... as previously described [21] In vitro discovery, validation and mapping of geneassociated SNPs PCR-amplified genomic amplicons were cloned and sequenced and DNA sequences were aligned essentially ... in the lower regions of LGs and and the upper region of LG5 of both F1 (NA6 × AU6) parental maps (Fig 3, 4, 5, 6) Comparative genetic mapping based on R gene loci Genetic mapping facilitated map ... functional role R gene sequence similarity and relationship to macrosynteny SNP discovery in F1(NA6xAU6) parental genotypes SNP validation and genetic mapping Association of SNPs with resistance...
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Báo cáo y học: "Validation and refinement of gene-regulatory pathways on a network of physical interactions" pps
Ngày tải lên :
14/08/2014, 14:21
... direction of change) between the Hughes et al swi4∆ profile and our new profile Expression changes were reproducible for 28 of 42 Msn4p-regulated genes, 11 of 29 Hap4p-regulated genes, and 64 of 119 ... set of all 15,116 pairwise protein-protein interactions recorded in the Database of Interacting Proteins as of April 2004 [19]; and a panel of mRNA expression profiles for 273 individual gene- deletion ... given by: The expression coherence of a set of genes measures whether the expression levels of these genes behave similarly in a particular experiment Each gene i in gene- deletion experiment e has...
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Báo cáo y học: "he discovery, positioning and verification of a set of transcription-associated motifs in vertebrates" pps
Ngày tải lên :
14/08/2014, 15:20
... discussion of evolutionary aspects see Wray et al [10] and for a review from the bioinformatics perspective see Wasserman and Sandelin [11] A motif is a subsequence of DNA of between and 20 base ... experimental evidence of binding affinities with this large excess of potential sites include epigenetic features, in particular chromatin modeling and methylation, and cooperative binding of complex combinations ... some probability of landing in a conserved region, so that, for a given overall occurrence of a motif, a proportion of the motifs randomly fall in these conserved regions The shape of the distribution...
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báo cáo hóa học:" Cross-cultural validation and analysis of responsiveness of the QUALIOST®: QUAlity of Life questionnaire In OSTeoporosis" pptx
Ngày tải lên :
20/06/2014, 15:20
... questionnaire (OFDQ) [4] focused mainly on pain and handicap rather than QoL and was not generated using patient interviews Based on the lack of availability of a suitable instrument, it was decided ... psychometric properties of both the QUALIOST® and SF-36 were assessed on baseline cross-sectional (N = 1486) and longitudinal data (N = 1288) Validation of the hypothesised structure of the QUALIOST® ... Groups of patients were defined according to the occurrence or not of the following types of fracture: all types of osteoporotic fracture Page of 10 (page number not for citation purposes) Health and...
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HIV INFECTION IN THE ERA OF HIGHLY ACTIVE ANTIRETROVIRAL TREATMENT AND SOME OF ITS ASSOCIATED COMPLICATIONS docx
Ngày tải lên :
27/06/2014, 18:20
... (2006) Safety and efficacy of extended-release niacin for 12 HIV Infection in the Era of Highly Active Antiretroviral Treatment and Some of Its Associated Complications the treatment of dyslipidaemia ... cohorts (for age, sex and cardiovascular risk factors) of HIV-infected patients and non-HIV 22 HIV Infection in the Era of Highly Active Antiretroviral Treatment and Some of Its Associated Complications ... which examined the effects of ART (two NRTIs and one NNRTI, two NRTIs and one PI or one PI and one NNRTI) on FMD, viral load and lipid profile showed that after 24 weeks of treatment there was an...
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Báo cáo y học: "Perspectives and limitations of gene expression profiling in rheumatology: new molecular strategies" doc
Ngày tải lên :
09/08/2014, 01:23
... and bone resorption, also demand the identification of targets to directly inhibit destruction and/ or to induce regeneration and repair A deeper knowledge of pathophysiological networks and gene ... personal knowledge and investigative capacity of the scientists and is susceptible to misinterpretation In the face of our limited knowledge of the role and function of most of the genes that we discover ... image analysis (millions of pixels per image) and comparisons of genes (several hundreds of thousands) Many efforts to distinguish signals from background and to identify and eliminate artefacts...
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Báo cáo y học: "MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays" pptx
Ngày tải lên :
09/08/2014, 20:20
... experimentally tested and further optimized based on the outcome of the experimental validation After completion of these six steps, the candidate biomarker is ready for application and further validation ... optimization and computational validation of candidate biomarker DNA methylation assay for the MGMT promoter, two key steps remain: experimental assay validation and experimental biomarker validation ... of DNA methylation over the score value of the regression formula (left) and the robustness of predictions in the face of increasing noise levels (right) these unsuccessful candidates early and...
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báo cáo khoa học: " Identification, utilisation and mapping of novel transcriptome-based markers from blackcurrant (Ribes nigrum)" pdf
Ngày tải lên :
11/08/2014, 11:21
... analysis software (Illumina BeadStudio v3.1), we were able to map 184 SNPs (48% of assayed SNPs) and 105 SNPs (27% of assayed SNPs) from two blackcurrant mapping populations, SCRI 9328 and MP7 ... identification of novel EST-SSRs which are proven robust marker types [10,16,17] To facilitate validation of these SNPs and SSRs, two segregating Page of 11 mapping populations and a diverse set of germplasm, ... the genetic maps in maize (63%) [27] and in two mapping populations of potato (43% and 48%) [30] There was good agreement of markers Page of 11 between maps with very little heterogeneity of recombination...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
Ngày tải lên :
12/08/2014, 03:20
... basis of our data this might be a consequence of the rapid conversion of CGA to DCQs as by means of an unknown downstream enzymatic step Indeed the involvement of the HQT gene in the profile of ... pathway of the species Methods The genetic mapping of biosynthetic pathway genes of known biochemical function can help unravel the complexity of plant secondary metabolism The precision of both ... understanding of the genetic basis of phenylpropanoid (PP) biosynthesis in C cardunculus; our future research activity will be focused on the analysis of the expression in vivo of both HQT and HCT,...
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Báo cáo y học: "Value and price of ventilator-associated pneumonia surveillance as a quality indicator" potx
Ngày tải lên :
13/08/2014, 20:21
... Care Med 2006, 34:211-218 Horan TC, Andrus M, Dudeck MA: CDC/NHSN surveillance definition of health care -associated infection and criteria for specific types of infections in the acute care setting ... Ventilator -associated pneumonia: caveats for benchmarking Intensive Care Med 2004, 30:996-997 doi:10.1186/cc8189 Cite this article as: Aardema LM, et al.: Value and price of ventilator -associated ... competing interests Page of Published: February 2010 References Klompas M: Unintended consequences in the drive for zero Thorax 2009, 64:463-465 Klompas M: The paradox of ventilator -associated pneumonia...
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Báo cáo y học: "Strategy for encoding and comparison of gene expression signatures" pps
Ngày tải lên :
14/08/2014, 07:22
... illustrating input of subject (Gene Expression Omnibus [GEO] dataset [GDS]) and query datasets, extraction of gene expression signatures, comparison with signature database, and generation of reports ... signature gene 'Concordant Similarity' is the number of concordant genes expressed as a percentage of the total number of genes in the signature 'Discordant Similarity' is the number of discordant genes ... described in the Materials and methods section (below) Design and validation of EXALT The EXALT system consists of four components (two program pipelines, SigDB, and search engine) and a web interface...
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Báo cáo y học: "Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish" pdf
Ngày tải lên :
14/08/2014, 08:20
... page) Phylogenetic tree of NACHT proteins shared by mammals and fish and diagram of their protein structures Phylogenetic tree of NACHT proteins shared by mammals and fish and diagram of their ... Figure Phylogenetic tree of the kinases Phylogenetic tree of the kinases Details of the tree are as in Figure Figure Phylogenetic tree of the interferon response factors Phylogenetic tree of the interferon ... STAT4 and STAT1 genes on human chromosome and Danio rerio chromosomes 22 and Synteny between regions containing STAT4 and STAT1 genes on human chromosome and Danio rerio chromosomes 22 and Genes...
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Báo cáo y học: " Validation and extension of an empirical Bayes method for SNP calling on Affymetrix microarrays" pot
Ngày tải lên :
14/08/2014, 08:21
... abbreviations for homozygous and heterozygous, respectively excluded from validation Distinct gold standards are generated for each chip and method In both the HapMap and replicate data validation, accuracy ... common diseases and 3,000 shared controls Nature 2007, 447:661-678 Diabetes Genetics Initiative of Broad Institute of Harvard and MIT, Lund University, and Novartis Institutes of BioMedical Research, ... developers of CRLMM, and have no plans of maintaining the original version, we avoid the use of yet another acronym and refer to our new algorithm as CRLMM as well A software implementation of CRLMM is...
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Báo cáo y học: " Advances in the genetics and epigenetics of gene regulation and human disease" pps
Ngày tải lên :
14/08/2014, 17:22
... several generations of offspring He and his colleagues postulate that maternal nutrition before and during pregnancy may affect the establishment of CpG methylation and the life-long expression of ... variety of human cancers, and are often associated with poor clinical outcome for the patient Target genes for amplified regions are often oncogenes, which may be used as therapeutic, prognostic and ... analysis of genes that are involved in cell-cycle control and cell-size regulation in Drosophila Using flow cytometry, Björklund and colleagues analyzed the RNAi-induced loss -of- function of 70% of...
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Accelerating Test, Validation and Debug of High Speed Serial Interfaces
Ngày tải lên :
09/04/2015, 17:22
... 155 6.2.1 Generating Random Variables 155 6.2.1.1 One Bit Random Number Generator 155 6.2.1.2 Multiple-Bit Random Number Generator 158 6.2.2 Gaussian Variable Generation ... consists of three processes: validation of the first set of fabricated devices, characterization of the devices under all settings across Process, Voltage and Temperature (PVT) corners, and production ... Accelerating Test, Validation and Debug of High Speed Serial Interfaces Yongquan Fan · Zeljko Zilic Accelerating Test, Validation and Debug of High Speed Serial Interfaces 123...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
Ngày tải lên :
16/02/2014, 15:20
... control of gene expression As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and translation rate is essential for the control of the expression of ... members of the SR proteins family, including ASF ⁄ SF2 and SRp20, have important roles in the cytoplasmic control of mRNA metabolism [15–17] Results Identification of TIAR -associated proteins The tandem ... characterization of TIAR-TAP protein and purification of TIAR-TAP complexes (A) Upper panel: RT-PCR analysis of exon 6A inclusion in minigene reporter transcript upon overexpression of TIAR-TAP protein...
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Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebrafish (Danio rerio) potx
Ngày tải lên :
31/03/2014, 07:20
... potential cis-acting regulatory elements of the promoter and gene linkage mapping of the B-FABP gene from zebrafish (Danio rerio) Materials and methods Zebrafish culture and breeding Zebrafish were purchased ... fragment upstream of exon of the B-FABP gene was obtained by linker-mediated PCR and cloned and sequenced The exon/intron organization of the zebrafish B-FABP gene (Fig 1), which consists of four exons ... zebrafish B-FABP gene Fig Structure of B-FABP genes from fishes and mammals (A) Comparison of the exon/intron organization of the zebrafish B-FABP gene (ZF) with the orthologous genes from human...
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Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio) pot
Ngày tải lên :
31/03/2014, 07:20
... patterns of the fabp3 genes from mammals and zebrafish were a result of evolutional divergence of transcriptional regulation Materials and methods Growth and maintenance of zebrafish adults and embryos ... single CRBP gene [12] Not only has the primary amino acid sequence of members of this multigene family been conserved over hundreds of millions of years, so has their gene structure All ILBP genes ... cDNA and the 5¢ upstream region of zebrafish fabp3 gene (A) The 5¢ upstream region of zebrafish fabp3 gene The coding sequence of the first exon is shown in uppercase letters and underlined and the...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
Ngày tải lên :
20/06/2014, 01:20
... prised of at least 14 IFNα genes and IFNα pseudogenes [57], and because the quantity of IFNα measured depends on the specificity of the detection antibody for these isoforms, detection of IFNα ... copies of RSV N gene Methods Viruses and cells Type I IFN-free virus stocks of recombinant RSV strain A2 (6340WT), 6340WT lacking the G protein gene (6340 G), and 6340WT lacking NS1 and NS2 genes ... SOCS3, pooled IFNα4 and IFNα9, and IFNβ1 gene expression assays (Applied Biosystems, Foster City, CA) and analyzed using MX300P software by Stratagene (La Jolla, CA) Each gene of interest was normalized...
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