diploidy disomy and genomic imprinting

... Chapter Detection of Uniparental Disomy and Imprinting by DNA Analysis 13 Mechanisms Generating Uniparently Disomy and Genomic Imprinting Disorders 25 Uniparental Disomy for Individual Human Chromosomes: ... represent the lineage of human genetics in Geneva Genomic Imprinting and Uniparental Disomy in Medicine will stand as a landmark contribution in biology and medicine The book thoroughly documents the ... recognition of the importance of genomic imprinting and uniparental disomy in human biology and medicine The book focuses on UPD but the ties to various other aspects of genomic imprinting are inevitable...

Ngày tải lên: 10/04/2014, 23:05

... maintenance Figure ART and possible imprinting defects ART and possible imprinting defects Possible interactions between different steps of assisted reproduction procedures and imprint establishment ... writing of this review and both read and approved the final manuscript Imprinting and placenta A critical way of regulating intrauterine development is through placental function and growth Most imprinted ... mechanisms underlying genomic imprinting is only at the beginning, there is a clear need to investigate and better understand the regulation of this process during fecundation and embryogenesis 14...

Ngày tải lên: 14/03/2014, 16:20

... edited by Amanda E I Proudfoot, Timothy N C Wells, and Christine Power, 2000 M E T H O D S I N M O L E C U L A R B I O L O G Y TM Genomic Imprinting Methods and Protocols Edited by Andrew Ward ... Data Genomic imprinting: methods and protocols / edited by Andrew Ward p cm (Methods in molecular biology ; v 181) Includes bibliographical references and index ISBN 0-89603-741-X (alk paper) Genomic ... useful for studying genomic imprinting They (1) are diploid and can be derived such that they contain only maternally and paternally derived genomes, termed parthenogenetic and androgenetic cell...

Ngày tải lên: 11/04/2014, 06:54

... metallothionein L Villarreal et al Fig Structure and synteny of the chicken, mouse and human genomic regions containing the MT genes The chromosome number and bands enclosing the MT cluster are indicated ... 81, 177–183 Fernando LP, Wei D & Andrews GK (1989) Structure and expression of chicken metallothionein J Nutr 119, 309–318 Dalton T, Paria BC, Fernando LP, Huet-Hudson YM, Dey SK & Andrews GK (1997) ... zinc and copper and the ESI-MS experimental error range prevented determination of the Zn:Cu ratio in the heterometallic Zn,Cu–ckMT species Synthesis of ckMT, ackMT and bckMT both in Znand in...

Ngày tải lên: 30/03/2014, 11:20

... edited by Amanda E I Proudfoot, Timothy N C Wells, and Christine Power, 2000 M E T H O D S I N M O L E C U L A R B I O L O G Y TM Genomic Imprinting Methods and Protocols Edited by Andrew Ward ... Data Genomic imprinting: methods and protocols / edited by Andrew Ward p cm (Methods in molecular biology ; v 181) Includes bibliographical references and index ISBN 0-89603-741-X (alk paper) Genomic ... useful for studying genomic imprinting They (1) are diploid and can be derived such that they contain only maternally and paternally derived genomes, termed parthenogenetic and androgenetic cell...

Ngày tải lên: 08/04/2014, 12:51

... effects and in some case even grand-maternal effects (88–92) The Taming of TEs and Their Technical Applications At present a deep and detailed understanding of the complex biology of mobile DNAs and ... between TEs and heterochromatin is still controversial In general, TEs and their derivatives are found as highly enriched clusters in genomic regions close to the centromere and telomere, and along ... D., and Langin, T., eds Evolution and Impact of Transposable Elements Kluwer Academic Publishers, Dordrecht, Netherlands, 1997 23 Capy, P., Gasperi, G., Biémont, C., and Bazin, C (2000) Stress and...

Ngày tải lên: 11/04/2014, 09:51

... distance between the shoot and the root (Greenwood, 1989) On the other hand, the mature characteristics, such as growth increments and chlorophyll content, foliar morphology and reproduction competence ... juvenility and rejuvenation of Cynara scolymus (Moncousin, 1982; Moncousin and Ducreux, 1984) However, the conclusions of Dalet and Cornu (1989) on Prunus avium not agree with the other findings GENOMIC ... juvenile and adult clones of walnut (Drouet et al, 1989) Recently, Hackett et al (1989) have shown that the juvenile and mature phases of Hedera helix can be characterized by polypeptides; and Bon and...

Ngày tải lên: 08/08/2014, 23:22

... sulfate and dehydroepiandro­ sterone sulfate, prostaglandins E2 and F2α, and urate [45,47,48] Since OAT4 is thought to be an asymmetric carrier, it may transport organic anions such as glutarate and ... for several organic anions and inorganic anions, such as lactate, pyrazine­ carboxylic acid and chloride, and is cis-inhibited by probenecid, benzbromarone, losartan, and lactate [18] Enomoto et ... terminus of URAT1, OAT4, and NPT1 binds with PDZK1 [81] URAT1 interacts with PDZK1 via PDZ domains 1, 2, and 4, while OAT4 interacts with PDZ domains and [56,57] OAT4, NPT1, and MRP4 also bind NHERF1...

Ngày tải lên: 11/08/2014, 12:20

... [54] and the Maori of New Zealand [55] Socio-economic and health infrastructure The strength of economic, scientific and health infra­ structures at study sites highlights the need for genomic ... design of genomic studies is perhaps the single most important factor that shapes the informed consent document and process Beliefs and concerns associated with different types of genomic research ... to combine and share large datasets generated by genomic projects has contributed significantly to the success stories enjoyed by the genomic scientific communities This is so because genomic techniques...

Ngày tải lên: 11/08/2014, 12:20

... transcript read coverage and location (Figures and 7) A “seed development coverage depth” track (Figures 6A and 7A) shows locations and counts of uniquely mapped HTTS reads, and a “seed development ... temporal and spatial distribution and partitioning of candidate gene expression that may govern the relationship between protein and oil accumulation in the developing soybean seed Processes and pathways ... relationship between protein and oil levels, and functional studies are under way to identify and validate the role(s) of candidate genes in the accumulation of protein and oil in the seed Methods...

Ngày tải lên: 12/08/2014, 03:21

... word clustering and help with the interpretation of statistical results, GB and AMA retrieve and organize the genome and methylation databases, and JLO developed the algorithm and wrote the manuscript ... have been measured and both absolute numbers and percentages are given might be biologically meaningful, we compared the percentage of methylated words (CAG and CTG) inside and outside of the ... Brigham and Woman’s Hospital, Harvard Medical School, Boston, MA 02115, USA Authors’ contributions MH developed and implemented the algorithm and wrote the manuscript (with JLO), PC and PB carried...

Ngày tải lên: 12/08/2014, 17:20

... inflation of genomic breeding values and reliabilities [5,11] Biases in genomic predictions can be related to incorrect weighting of polygenic and genomic components The combined pedigree -genomic ... pedigree and genomic relationship coefficients Table Variance components estimates for litter size using pedigree and genomic relationship coefficients Additive Variance (ste1) Breeding values and ... species, and pedigree and genomic information have to be combined Estimates of relationships are always relative to an arbitrary base population in which the average relationship is zero Genomic and...

Ngày tải lên: 14/08/2014, 13:21

... usage, genomic GC content and evolutionary rates refereed research The expression of developmental-pivotal genes (regulated up and down) and developmental-specific genes (regulated on and off) ... recombination gene Fold and GCg gene gene between composition bination rate, offile expression are developmental-pivotal Levelsand datarate and andcorrelated with codon codon Clickgenes andexpression are ... developmental-pivotal and developmental-specific genes will deepen our understanding of the higher Ks and Ka/Ks in these genes reports Comparisons between developmental-pivotal genes and developmental-specific...

Ngày tải lên: 14/08/2014, 20:22

... central nodes: IL-10 and IFN-γ Upon stimulation by anti-CD3/CD28 and IL-2, IL-10 and IFN-γ are highly upregulated (by 47- and 51-fold, respectively; Table 4) These proteins and pathways are known ... as described in Materials and methods and the results are summarized in Table As expected, the expression of thousands of genes was changed by the expansion protocol and the activation protocol ... ≤0.01 and fold change ≥5 are included in this map Data for each gene are standardized separately before being plotted, as is standard in drawing heat maps, so that all genes have a similar scale and...

Ngày tải lên: 14/08/2014, 20:22

Ngày tải lên: 14/11/2014, 08:19

... elements 25 2.4 Genomic imprinting and RNA-directed DNA methylation 27 2.5 Imprinting, a by-product of the global reprogramming? 30 iii Biological significance and evolution of imprinting ... convergent evolution of imprinting mechanisms between plants and mammals The physiological significance and evolutionary origin of imprinting are still unclear but in plants, imprinting may be the ... CHAPTER 2: GENOMIC IMPRINTING AND RNA-DIRECTED DNA METHYLATION IN ARABIDOPSIS 39 2.1 Introduction 40 2.2 Results 43 2.2.1 SDC is silenced in somatic tissues and expressed...

Ngày tải lên: 11/09/2015, 10:14

... cells and cancer cells is of particular interest for researchers who seek to understand the processes of cellular differentiation and carcinogenesis (9) And the transcriptome of human oocytes and ... reads and paired-end information is able to construct a cleaner splicing graph and generate more accurate and reliable transcriptome We follow the classical overlap-layout-consensus scheme and ... RNAs and small RNAs; to determine the transcriptional structure of genes, in terms of their start sites, 5’ and 3’ ends, splicing patterns and other post-transcriptional modifications; and to...

Ngày tải lên: 01/10/2015, 17:28

... that human and chimpanzee genes contain more LIRs than rhesus monkey and mouse orthologs Our data suggest that most of the LIRs shared by human and chimpanzee orthologs were developed and maintained ... having humanand chimpanzee-specific LIRs (i.e rhesus monkey and mouse orthologs not have specific LIRs) and the mouse orthologs having mouse-specific LIRs To make a reference dataset, we randomly selected ... orthologs Results Characters and distribution of LIRs in human autosomes We identified 2551 LIRs in human autosomes and approximately 87% of them have a short spacer (0–9 bp) and arm (31–59 bp) (Fig...

Ngày tải lên: 07/03/2014, 00:20

... A11 and BM CGTases determined by the 2,4,6-trinitrobenzene sulfonic acid (TNBS) assay were 62% and 65%, respectively The remaining activities of the derivatized A11 and BM CGTases were 90% and ... cyclohexane and with 50 mm potassium phosphate buffer (pH 6.0) (3 · 10 mL) and lyophilized The protein amounts and enzyme activities were monitored during the different steps Effect of pH and temperature ... solvent The ligand is removed in the final step, and the CLIP enzyme can be used either in aqueous medium or organic solvent The CLIP enzymes show altered substrate or product specificity and enhanced...

Ngày tải lên: 07/03/2014, 10:20

... FABP3 and the four Antarctic fish H8-FABPs, along with other fish and mammalian FABP3s, formed a separate clade (Fig 4) The phylogenetic analysis resolved fish FABP4 (and H6-FABPs) and FABP3 (and ... and FABP4 show additional similarities in their tertiary structures, related to their ligand-binding specificity and affinity [10] In addition to the similar threedimensional structure and ligand-binding ... specificity and affinity of FABP3 and FABP4, the transcripts and proteins of the two paralogous genes exist in multiple tissues and are colocalized in several mammalian tissues, including mammary gland...

Ngày tải lên: 07/03/2014, 10:20